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The map label for this gene is nylA [H]

Identifier: 148652434

GI number: 148652434

Start: 748211

End: 749701

Strand: Direct

Name: nylA [H]

Synonym: PsycPRwf_0622

Alternate gene names: 148652434

Gene position: 748211-749701 (Clockwise)

Preceding gene: 148652433

Following gene: 148652436

Centisome position: 25.12

GC content: 49.9

Gene sequence:

>1491_bases
ATGTTTAAAGAATACCCGCAATATGATGCCATAGGATTGGCAGCGCTTATTAGATCCGGTGAAATCAGTGCCAAACAGGC
TCTAGATGCGGCTATATATCAGGCCAATAGGCTAAATCCTAAGCTTAACGCCATCATTCATCGATTTGATGAGCGGGCAT
TTAACGAAGTGCAGCGAGGTTTGCCTCAAGGCATATTCACTGGGGTGCCTTATTTATTAAAAGATTTAAATTACTTATTT
GCCGATGAGCCGATTAAAATGGGCAGCCGCAGCGTTCATATTGTGCCAGAGCAAGACAACGAGCTGGTAAAACGCATGCG
AGCCACTGGCGTCAATACCTTTGGTAAAACCAATACCCCAGAGTTTGGCTTAATTATTACCACAGAGCCTAAGTCTTATG
GCGCCACCCATAATCCATTTAAAAAAGGCTATAGCAGCGGCGGCTCATCAGGTGGCAGTGCTGCAGCAGTGAGTGCCGGT
ATCGTGCCTATGGCAGGTGGCGGTGATGGGGGTGGCTCTATTCGCTTCCCATCCGCTTGGTGCGGCGTGTTTGGCTTAAA
GCCCAGTCGTGGCCGCAATCCCAGTGGTCCTCTCTATGCTGAAGGCTGGGAGGGAGCTGTGGCCGACCATGTGATTACCC
GCAGTGTGCGCGACAGTGCCGCCATGCTTGATGCCACGGCTGGTAGAGAGGTTGGCTCAGCGTTTGTGACACAGGCCTCT
GATAACGGCCAGTTTTTACAGGCCACACAGACACCGTCTAAACCGTTAAAGATTGCGCTGATGCAAGCGCCTTTGTTTCC
AGGTGCCACAGTAGACAAAGAGGTGCTGGCGGTACTAGAAAAAACAGCACGGCAGCTTGAAGACATGGGGCACACAATAG
ACTATGCCGCCCCAAACATTGATATTGACCGCATGTGGCGAGATTTTCTTACAGTGGTGTGTGCTCATACGGCGCATTTT
GTGGAAGATTTGTCGCTGCGCTATGGGCCGCAGCAAGTTGCTAATCTGGAGCCACAGACTCGAAATATGGTCATGATAGG
ACGCTCGCTAAGTGTGATTGACCTACTTAAAGCCAAGCAAGGCTGGCATGACGTACAGTACCAGACCGGACTGATTCTTG
AGCAGTACGATATGATTTTATGCCCCACGGTGCCGACGCCTGCAGTTAAGCATGGGGTGCTGCCGCCCAGTCGTATTGAT
GAGCTACTGCTTAATGTTAGCGGCTTGATTGATCGAGGCGTTAATCTGGGTAAATGGATTTATAACTCAAGCTTGGTCGA
GAAGCTAAGCGCACCGGTATTAAGCAAAATGGCCTTTACGACTTTGGGCAATATTACAGGTCTACCGGCCATGTCAGTCC
CGCTAGGTATGAGCAATAAGGGCTTACCGATTGGCATGCAATTTATTGGGCGTATGAATGATGAGGCGAGGCTTTTTGCT
TTGGCAGCAGACTTTGAGCGGTCAGGACTATTTACCGATCCGGCCATTTAA

Upstream 100 bases:

>100_bases
TTAAGATAAGCTAGGATAAAGATTTGCCTAACTATTTTGTCAAAATGACCTAAGCTCTCAAAAGAGCCAATTTACCCCCC
AAACACGAGCAGAGGACGCT

Downstream 100 bases:

>100_bases
TACTCTCTACGTTGATTTGTTAAGGTAGCCACTATAAACAGAGCCTATGACGATATGTATTAGGCTCTTTATAAATAGCT
TTAGTCCCCCTAGACCACCT

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MFKEYPQYDAIGLAALIRSGEISAKQALDAAIYQANRLNPKLNAIIHRFDERAFNEVQRGLPQGIFTGVPYLLKDLNYLF
ADEPIKMGSRSVHIVPEQDNELVKRMRATGVNTFGKTNTPEFGLIITTEPKSYGATHNPFKKGYSSGGSSGGSAAAVSAG
IVPMAGGGDGGGSIRFPSAWCGVFGLKPSRGRNPSGPLYAEGWEGAVADHVITRSVRDSAAMLDATAGREVGSAFVTQAS
DNGQFLQATQTPSKPLKIALMQAPLFPGATVDKEVLAVLEKTARQLEDMGHTIDYAAPNIDIDRMWRDFLTVVCAHTAHF
VEDLSLRYGPQQVANLEPQTRNMVMIGRSLSVIDLLKAKQGWHDVQYQTGLILEQYDMILCPTVPTPAVKHGVLPPSRID
ELLLNVSGLIDRGVNLGKWIYNSSLVEKLSAPVLSKMAFTTLGNITGLPAMSVPLGMSNKGLPIGMQFIGRMNDEARLFA
LAADFERSGLFTDPAI

Sequences:

>Translated_496_residues
MFKEYPQYDAIGLAALIRSGEISAKQALDAAIYQANRLNPKLNAIIHRFDERAFNEVQRGLPQGIFTGVPYLLKDLNYLF
ADEPIKMGSRSVHIVPEQDNELVKRMRATGVNTFGKTNTPEFGLIITTEPKSYGATHNPFKKGYSSGGSSGGSAAAVSAG
IVPMAGGGDGGGSIRFPSAWCGVFGLKPSRGRNPSGPLYAEGWEGAVADHVITRSVRDSAAMLDATAGREVGSAFVTQAS
DNGQFLQATQTPSKPLKIALMQAPLFPGATVDKEVLAVLEKTARQLEDMGHTIDYAAPNIDIDRMWRDFLTVVCAHTAHF
VEDLSLRYGPQQVANLEPQTRNMVMIGRSLSVIDLLKAKQGWHDVQYQTGLILEQYDMILCPTVPTPAVKHGVLPPSRID
ELLLNVSGLIDRGVNLGKWIYNSSLVEKLSAPVLSKMAFTTLGNITGLPAMSVPLGMSNKGLPIGMQFIGRMNDEARLFA
LAADFERSGLFTDPAI
>Mature_496_residues
MFKEYPQYDAIGLAALIRSGEISAKQALDAAIYQANRLNPKLNAIIHRFDERAFNEVQRGLPQGIFTGVPYLLKDLNYLF
ADEPIKMGSRSVHIVPEQDNELVKRMRATGVNTFGKTNTPEFGLIITTEPKSYGATHNPFKKGYSSGGSSGGSAAAVSAG
IVPMAGGGDGGGSIRFPSAWCGVFGLKPSRGRNPSGPLYAEGWEGAVADHVITRSVRDSAAMLDATAGREVGSAFVTQAS
DNGQFLQATQTPSKPLKIALMQAPLFPGATVDKEVLAVLEKTARQLEDMGHTIDYAAPNIDIDRMWRDFLTVVCAHTAHF
VEDLSLRYGPQQVANLEPQTRNMVMIGRSLSVIDLLKAKQGWHDVQYQTGLILEQYDMILCPTVPTPAVKHGVLPPSRID
ELLLNVSGLIDRGVNLGKWIYNSSLVEKLSAPVLSKMAFTTLGNITGLPAMSVPLGMSNKGLPIGMQFIGRMNDEARLFA
LAADFERSGLFTDPAI

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=524, Percent_Identity=25.381679389313, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI222831590, Length=533, Percent_Identity=23.827392120075, Blast_Score=85, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556264, Length=227, Percent_Identity=29.0748898678414, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17537465, Length=197, Percent_Identity=29.4416243654822, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=434, Percent_Identity=23.2718894009217, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=445, Percent_Identity=23.5955056179775, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=443, Percent_Identity=23.9277652370203, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17538254, Length=438, Percent_Identity=26.2557077625571, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=474, Percent_Identity=24.2616033755274, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI21356731, Length=509, Percent_Identity=25.3438113948919, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24644968, Length=518, Percent_Identity=24.3243243243243, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24652985, Length=535, Percent_Identity=23.9252336448598, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19922090, Length=535, Percent_Identity=23.9252336448598, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24652981, Length=535, Percent_Identity=23.9252336448598, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24652983, Length=535, Percent_Identity=23.9252336448598, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI161078093, Length=508, Percent_Identity=25.1968503937008, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI45550774, Length=241, Percent_Identity=26.9709543568465, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24648113, Length=516, Percent_Identity=24.8062015503876, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24648435, Length=241, Percent_Identity=26.9709543568465, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24648437, Length=241, Percent_Identity=26.9709543568465, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 53592; Mature: 53592

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKEYPQYDAIGLAALIRSGEISAKQALDAAIYQANRLNPKLNAIIHRFDERAFNEVQRG
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
LPQGIFTGVPYLLKDLNYLFADEPIKMGSRSVHIVPEQDNELVKRMRATGVNTFGKTNTP
CCHHHHHHHHHHHHHHHHHHCCCCHHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCC
EFGLIITTEPKSYGATHNPFKKGYSSGGSSGGSAAAVSAGIVPMAGGGDGGGSIRFPSAW
CEEEEEEECCHHCCCCCCHHHHHHCCCCCCCCCHHHHHCCCEEECCCCCCCCCEECCHHH
CGVFGLKPSRGRNPSGPLYAEGWEGAVADHVITRSVRDSAAMLDATAGREVGSAFVTQAS
HHHCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEECC
DNGQFLQATQTPSKPLKIALMQAPLFPGATVDKEVLAVLEKTARQLEDMGHTIDYAAPNI
CCCCEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCC
DIDRMWRDFLTVVCAHTAHFVEDLSLRYGPQQVANLEPQTRNMVMIGRSLSVIDLLKAKQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCEEEECCCHHHHHHHHHHC
GWHDVQYQTGLILEQYDMILCPTVPTPAVKHGVLPPSRIDELLLNVSGLIDRGVNLGKWI
CCCCCHHHCCCEEECCCEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHH
YNSSLVEKLSAPVLSKMAFTTLGNITGLPAMSVPLGMSNKGLPIGMQFIGRMNDEARLFA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEE
LAADFERSGLFTDPAI
EEECCCCCCCCCCCCC
>Mature Secondary Structure
MFKEYPQYDAIGLAALIRSGEISAKQALDAAIYQANRLNPKLNAIIHRFDERAFNEVQRG
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
LPQGIFTGVPYLLKDLNYLFADEPIKMGSRSVHIVPEQDNELVKRMRATGVNTFGKTNTP
CCHHHHHHHHHHHHHHHHHHCCCCHHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCC
EFGLIITTEPKSYGATHNPFKKGYSSGGSSGGSAAAVSAGIVPMAGGGDGGGSIRFPSAW
CEEEEEEECCHHCCCCCCHHHHHHCCCCCCCCCHHHHHCCCEEECCCCCCCCCEECCHHH
CGVFGLKPSRGRNPSGPLYAEGWEGAVADHVITRSVRDSAAMLDATAGREVGSAFVTQAS
HHHCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEECC
DNGQFLQATQTPSKPLKIALMQAPLFPGATVDKEVLAVLEKTARQLEDMGHTIDYAAPNI
CCCCEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCC
DIDRMWRDFLTVVCAHTAHFVEDLSLRYGPQQVANLEPQTRNMVMIGRSLSVIDLLKAKQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCEEEECCCHHHHHHHHHHC
GWHDVQYQTGLILEQYDMILCPTVPTPAVKHGVLPPSRIDELLLNVSGLIDRGVNLGKWI
CCCCCHHHCCCEEECCCEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHH
YNSSLVEKLSAPVLSKMAFTTLGNITGLPAMSVPLGMSNKGLPIGMQFIGRMNDEARLFA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEE
LAADFERSGLFTDPAI
EEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]