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The map label for this gene is ddl

Identifier: 148652338

GI number: 148652338

Start: 628973

End: 629971

Strand: Direct

Name: ddl

Synonym: PsycPRwf_0526

Alternate gene names: 148652338

Gene position: 628973-629971 (Clockwise)

Preceding gene: 148652337

Following gene: 148652339

Centisome position: 21.11

GC content: 47.15

Gene sequence:

>999_bases
ATGCCCATGACTATGACTCAATCTGCTACCAACCCAACTGCAACCCCTGTATCTGCTAACAAAGCTTCTGCTAACGCCGC
CACGACTCAAGATCCAAGCATCTTTGGTAAGGTCGCCGTGGTCTTTGGGGGTAATAGCAATGAGCGAGCAGTGTCATTAG
ACAGTGGCAATGCGGTATTACAGGCGCTACAGTCACAGGGCATCGATGCCACTCACTTTGACCCTAAAGACCAAGATGTG
ACTGAATTAAAAAATTACGACCGTGTATTTAATGTGCTACATGGCCGCGGCGGTGAAGATGGTCAGTTACAAGGCCTGTT
AGATTGGCTGGGTCTACCCCAGACAGGATCCGGTGTTTTGGCATCAGCGATTGGTATGGACAAAGTACGTACTAAGCAAT
TGTGGCACGGCTGTGGATTATCTACTGCGCCTTTTGCAGTCTTGGGCGCTGACACAGACTGGCAGCAAATCGTTAATACT
TTGGGTCTGCCTTTAATTGTAAAGCCAGTGCATGAAGGCTCAAGTATTGGTATGAGCAAGGTGAATACGTTAGATGAGCT
GCCAAAAGCCTATGAAGTGGCAGCAGGGTGTGGTGATGTGGTGATGGCAGAAAAATGGATTACTGGCCGTGAGTTTACCA
TCGTTATTATCGATGATGAGGCTTATCCAGTTATTCGCCTGCAGCCTGCCGACATCTCTAATTTCTACGACTATGAGGCC
AAATACAATCGTAACGATACTCAGTACCATATTCCTTGTGGCTTAAGCGCAGAAGAAGAAGCACATTTACAGGCACTAAG
CTTGGCGGCATTTAAGGCAGTAGACGCCAAAGGCTGGGGCCGTATTGATGCTATGCAAGACGAAGAAGGTAACTTTTGGC
TATTAGAGATTAATACCGTACCTGGTATGACCAGTCACAGCTTAGTACCTATGGCAGCTAAAGCACGTGGGATGAATTTT
GAACAGCTATGTTGGCATATCTTAGCCCAGACGCTGTAA

Upstream 100 bases:

>100_bases
AATTTTTATCTATACTATTTACGTTGTGACTAAGCTGACTGGTCTTATTGGCCTTGCTGGGTGTCACGGCTTTGATTATT
GACCCTTTTTACCATTTAAG

Downstream 100 bases:

>100_bases
GGGTTAACAGGGCTAGTGTGGTTAAATAATCAGATAAATAAGGCAAGGCTAACCACCACGCGGATCTGATTAAAACACAC
CCATTTAAACCCAAAACCAA

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MPMTMTQSATNPTATPVSANKASANAATTQDPSIFGKVAVVFGGNSNERAVSLDSGNAVLQALQSQGIDATHFDPKDQDV
TELKNYDRVFNVLHGRGGEDGQLQGLLDWLGLPQTGSGVLASAIGMDKVRTKQLWHGCGLSTAPFAVLGADTDWQQIVNT
LGLPLIVKPVHEGSSIGMSKVNTLDELPKAYEVAAGCGDVVMAEKWITGREFTIVIIDDEAYPVIRLQPADISNFYDYEA
KYNRNDTQYHIPCGLSAEEEAHLQALSLAAFKAVDAKGWGRIDAMQDEEGNFWLLEINTVPGMTSHSLVPMAAKARGMNF
EQLCWHILAQTL

Sequences:

>Translated_332_residues
MPMTMTQSATNPTATPVSANKASANAATTQDPSIFGKVAVVFGGNSNERAVSLDSGNAVLQALQSQGIDATHFDPKDQDV
TELKNYDRVFNVLHGRGGEDGQLQGLLDWLGLPQTGSGVLASAIGMDKVRTKQLWHGCGLSTAPFAVLGADTDWQQIVNT
LGLPLIVKPVHEGSSIGMSKVNTLDELPKAYEVAAGCGDVVMAEKWITGREFTIVIIDDEAYPVIRLQPADISNFYDYEA
KYNRNDTQYHIPCGLSAEEEAHLQALSLAAFKAVDAKGWGRIDAMQDEEGNFWLLEINTVPGMTSHSLVPMAAKARGMNF
EQLCWHILAQTL
>Mature_331_residues
PMTMTQSATNPTATPVSANKASANAATTQDPSIFGKVAVVFGGNSNERAVSLDSGNAVLQALQSQGIDATHFDPKDQDVT
ELKNYDRVFNVLHGRGGEDGQLQGLLDWLGLPQTGSGVLASAIGMDKVRTKQLWHGCGLSTAPFAVLGADTDWQQIVNTL
GLPLIVKPVHEGSSIGMSKVNTLDELPKAYEVAAGCGDVVMAEKWITGREFTIVIIDDEAYPVIRLQPADISNFYDYEAK
YNRNDTQYHIPCGLSAEEEAHLQALSLAAFKAVDAKGWGRIDAMQDEEGNFWLLEINTVPGMTSHSLVPMAAKARGMNFE
QLCWHILAQTL

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=301, Percent_Identity=54.1528239202658, Blast_Score=314, Evalue=4e-87,
Organism=Escherichia coli, GI1786579, Length=347, Percent_Identity=29.3948126801153, Blast_Score=145, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_PSYWF (A5WCU0)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001279431.1
- ProteinModelPortal:   A5WCU0
- SMR:   A5WCU0
- STRING:   A5WCU0
- GeneID:   5205497
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_0526
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   VPYTGSD
- ProtClustDB:   PRK01372
- BioCyc:   PSP56811:PSYCPRWF_0526-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 35849; Mature: 35718

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMTMTQSATNPTATPVSANKASANAATTQDPSIFGKVAVVFGGNSNERAVSLDSGNAVL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCHHHH
QALQSQGIDATHFDPKDQDVTELKNYDRVFNVLHGRGGEDGQLQGLLDWLGLPQTGSGVL
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHH
ASAIGMDKVRTKQLWHGCGLSTAPFAVLGADTDWQQIVNTLGLPLIVKPVHEGSSIGMSK
HHHHCHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHCCCCEEEEECCCCCCCCHHH
VNTLDELPKAYEVAAGCGDVVMAEKWITGREFTIVIIDDEAYPVIRLQPADISNFYDYEA
HHHHHHHHHHHHHHCCCCCEEEHHHCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCC
KYNRNDTQYHIPCGLSAEEEAHLQALSLAAFKAVDAKGWGRIDAMQDEEGNFWLLEINTV
EECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCEEEEEEECC
PGMTSHSLVPMAAKARGMNFEQLCWHILAQTL
CCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PMTMTQSATNPTATPVSANKASANAATTQDPSIFGKVAVVFGGNSNERAVSLDSGNAVL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCHHHH
QALQSQGIDATHFDPKDQDVTELKNYDRVFNVLHGRGGEDGQLQGLLDWLGLPQTGSGVL
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHH
ASAIGMDKVRTKQLWHGCGLSTAPFAVLGADTDWQQIVNTLGLPLIVKPVHEGSSIGMSK
HHHHCHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHCCCCEEEEECCCCCCCCHHH
VNTLDELPKAYEVAAGCGDVVMAEKWITGREFTIVIIDDEAYPVIRLQPADISNFYDYEA
HHHHHHHHHHHHHHCCCCCEEEHHHCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCC
KYNRNDTQYHIPCGLSAEEEAHLQALSLAAFKAVDAKGWGRIDAMQDEEGNFWLLEINTV
EECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCEEEEEEECC
PGMTSHSLVPMAAKARGMNFEQLCWHILAQTL
CCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA