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The map label for this gene is citE [H]

Identifier: 148652323

GI number: 148652323

Start: 609840

End: 610721

Strand: Direct

Name: citE [H]

Synonym: PsycPRwf_0511

Alternate gene names: 148652323

Gene position: 609840-610721 (Clockwise)

Preceding gene: 148652319

Following gene: 148652325

Centisome position: 20.47

GC content: 47.96

Gene sequence:

>882_bases
ATGAGCGACTCTTATCGCATAACGCCAATCGCCAGCTCTGGTACAGGCAGCCGGTCAATCGTCAGCCCACGCAGTTGGTT
ATTCGTCCCCGCCACTTATCCAGAGCGATTTAATAAAGCATTAAACAGTGGGGCGGAAGCGGTCATTATTGATTTAGAAG
ATGCTGTGGCCTTGGACCAAAAGCGCCAGGCACGTGAGAATATAGATGAGTTTTTTTTACAATTAGAGTCTGATAATGTG
AGAGCCAGTGTTTGGCTGCGCCTTAATAATGATGAGCAATTGGCTGAGGATTTAGTGCTATGTAAGCAGCTGACCTCAAG
TAAGTTGGTGTCCGGCGTGCTGCTGCCCAAGCTGACCTCAAGTGCGCCAGTCACTCAAGTATGGGAGCAAACGGGGTTAA
CTGTTGTGGGGCAGATTGAGTCAGCTCTAGGATTAAGTCAGTTAAACCAAATCACCCAAAGCAAGGGGCTTATGGCGCTA
AGCTATGGCCGCCTTGATATTAGAAATGAGTTGTCATTACGCGCAGGCAGTCAAGCTGAGCAAGACTTTTTTCGCCAGCT
GCGCGTCACTTTATTATTAGCCAGTAAAGCCAATGGATTACAAGCCCCGATCGAGAGTATTTATGCCGATTTTAAAGATG
AGGCAGGCATGCAAATGGCGGCTGAATACGCTTCTGACTTAGGCTTCTCAGGTATGCTGTGTATCCACCCCAAGCAGGTG
GCCTTAGTAAATACAGCCTTTACCCCCAGCCAAGCTCAGCTTGAGTTTGCCCAACAAGTAATCGACTATTATCAGCGCAC
TGGTGAGAGCATCTTTGCCATAGACGGGGTCATGGTAGATTTACCGGTGATTTTGCAGTGTCAAACACTGTTGTCTGCTT
AG

Upstream 100 bases:

>100_bases
TACTTGGTAGTTTGTCTGTTTTGGCTCTGAGCTGTAATCATTTTATTGTTAATTTTAATAAATATACCTATAAACCATGA
AGGGTAAAAAAGGATAAAAA

Downstream 100 bases:

>100_bases
AGTCAGACACTGCATTATTTAACAACGCTGCATTACTTAGCCACCAAGGTGCTGACTAAGTCACGCAGCGTGTCCCATTC
AAAATACTCTCCTGGATCCG

Product: HpcH/HpaI aldolase

Products: acetate; oxaloacetate

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MSDSYRITPIASSGTGSRSIVSPRSWLFVPATYPERFNKALNSGAEAVIIDLEDAVALDQKRQARENIDEFFLQLESDNV
RASVWLRLNNDEQLAEDLVLCKQLTSSKLVSGVLLPKLTSSAPVTQVWEQTGLTVVGQIESALGLSQLNQITQSKGLMAL
SYGRLDIRNELSLRAGSQAEQDFFRQLRVTLLLASKANGLQAPIESIYADFKDEAGMQMAAEYASDLGFSGMLCIHPKQV
ALVNTAFTPSQAQLEFAQQVIDYYQRTGESIFAIDGVMVDLPVILQCQTLLSA

Sequences:

>Translated_293_residues
MSDSYRITPIASSGTGSRSIVSPRSWLFVPATYPERFNKALNSGAEAVIIDLEDAVALDQKRQARENIDEFFLQLESDNV
RASVWLRLNNDEQLAEDLVLCKQLTSSKLVSGVLLPKLTSSAPVTQVWEQTGLTVVGQIESALGLSQLNQITQSKGLMAL
SYGRLDIRNELSLRAGSQAEQDFFRQLRVTLLLASKANGLQAPIESIYADFKDEAGMQMAAEYASDLGFSGMLCIHPKQV
ALVNTAFTPSQAQLEFAQQVIDYYQRTGESIFAIDGVMVDLPVILQCQTLLSA
>Mature_292_residues
SDSYRITPIASSGTGSRSIVSPRSWLFVPATYPERFNKALNSGAEAVIIDLEDAVALDQKRQARENIDEFFLQLESDNVR
ASVWLRLNNDEQLAEDLVLCKQLTSSKLVSGVLLPKLTSSAPVTQVWEQTGLTVVGQIESALGLSQLNQITQSKGLMALS
YGRLDIRNELSLRAGSQAEQDFFRQLRVTLLLASKANGLQAPIESIYADFKDEAGMQMAAEYASDLGFSGMLCIHPKQVA
LVNTAFTPSQAQLEFAQQVIDYYQRTGESIFAIDGVMVDLPVILQCQTLLSA

Specific function: May play a role in fatty acid biosynthesis (Potential) [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit-like subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=309, Percent_Identity=25.8899676375405, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI87081764, Length=298, Percent_Identity=27.1812080536913, Blast_Score=84, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17557458, Length=299, Percent_Identity=28.0936454849498, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 32144; Mature: 32013

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSYRITPIASSGTGSRSIVSPRSWLFVPATYPERFNKALNSGAEAVIIDLEDAVALDQ
CCCCEEEEEEECCCCCCCCEECCCCEEEEECCCHHHHHHHHCCCCCEEEEECHHHHHHHH
KRQARENIDEFFLQLESDNVRASVWLRLNNDEQLAEDLVLCKQLTSSKLVSGVLLPKLTS
HHHHHHHHHHHHEEECCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SAPVTQVWEQTGLTVVGQIESALGLSQLNQITQSKGLMALSYGRLDIRNELSLRAGSQAE
CCCHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCEEEECCCEECCCCCCCCCCCHHH
QDFFRQLRVTLLLASKANGLQAPIESIYADFKDEAGMQMAAEYASDLGFSGMLCIHPKQV
HHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEECCCEE
ALVNTAFTPSQAQLEFAQQVIDYYQRTGESIFAIDGVMVDLPVILQCQTLLSA
EEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SDSYRITPIASSGTGSRSIVSPRSWLFVPATYPERFNKALNSGAEAVIIDLEDAVALDQ
CCCEEEEEEECCCCCCCCEECCCCEEEEECCCHHHHHHHHCCCCCEEEEECHHHHHHHH
KRQARENIDEFFLQLESDNVRASVWLRLNNDEQLAEDLVLCKQLTSSKLVSGVLLPKLTS
HHHHHHHHHHHHEEECCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SAPVTQVWEQTGLTVVGQIESALGLSQLNQITQSKGLMALSYGRLDIRNELSLRAGSQAE
CCCHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCEEEECCCEECCCCCCCCCCCHHH
QDFFRQLRVTLLLASKANGLQAPIESIYADFKDEAGMQMAAEYASDLGFSGMLCIHPKQV
HHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEECCCEE
ALVNTAFTPSQAQLEFAQQVIDYYQRTGESIFAIDGVMVDLPVILQCQTLLSA
EEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]