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The map label for this gene is gloA [H]

Identifier: 148652218

GI number: 148652218

Start: 498216

End: 498761

Strand: Direct

Name: gloA [H]

Synonym: PsycPRwf_0406

Alternate gene names: 148652218

Gene position: 498216-498761 (Clockwise)

Preceding gene: 148652217

Following gene: 148652219

Centisome position: 16.72

GC content: 43.59

Gene sequence:

>546_bases
ATGTCTATTAATTATGTTGACCAACCCAAAGGCTCCGTTGTGGTAGATGGCGTACAGCCGATTGCTGCGGCAACCACAGG
TTATACTTTTAACCACACCATGCTACGGGTTAAAGACCCCAAAGCGTCGCTTGAATTTTATACCGGTGTGCTGGGTATGA
CGCTTTTGACGGTAAAAAAATTCCCAGAAATGGCGTTTGATTTGTACTTTTTGGCCAAATTAACCGATGACGAGCGTGCC
AATTTGCCAGAAAACGAAGAATTAGCGATTTATACTTTCCGTCAACGTGGTATTTTAGAGTTGACCCATAACTATGGCAC
CGAGACCCAGCCAGGACGTATTTATCATGACGGCAATGCTGAGCCACAAGGTTTTGGTCACATTTGCTTTAGCGTCCCTG
ACTTAACTGCCGCCGTAGAGTGGTTTGATAAAAACAACGTAGAGTTCAAAAAACGCCCAGAAGAGGGAAAAATGAAGAAC
GTGGTGTTTATCAAAGATGTTGATGGTTACTGGATTGAAGTGGTACAAGCTGATCTTATGGGTTAA

Upstream 100 bases:

>100_bases
TATAGGGTAACCGGCTTGATTCAAATGCCTTTTAGGCTAGGTAGTCGTGATACTAGATACTGTTTTTATCAGTGATGTAT
TTGATATAAAGGATGTAATA

Downstream 100 bases:

>100_bases
TAGATTGTACAAGTGCATACTGTGCCTCTAAAGCGTTATAAACTTAACACGTTTTATAAAGGTCACAAAGAGGTATTAAC
ACCGGTTTTGCTAACAGTAG

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MSINYVDQPKGSVVVDGVQPIAAATTGYTFNHTMLRVKDPKASLEFYTGVLGMTLLTVKKFPEMAFDLYFLAKLTDDERA
NLPENEELAIYTFRQRGILELTHNYGTETQPGRIYHDGNAEPQGFGHICFSVPDLTAAVEWFDKNNVEFKKRPEEGKMKN
VVFIKDVDGYWIEVVQADLMG

Sequences:

>Translated_181_residues
MSINYVDQPKGSVVVDGVQPIAAATTGYTFNHTMLRVKDPKASLEFYTGVLGMTLLTVKKFPEMAFDLYFLAKLTDDERA
NLPENEELAIYTFRQRGILELTHNYGTETQPGRIYHDGNAEPQGFGHICFSVPDLTAAVEWFDKNNVEFKKRPEEGKMKN
VVFIKDVDGYWIEVVQADLMG
>Mature_180_residues
SINYVDQPKGSVVVDGVQPIAAATTGYTFNHTMLRVKDPKASLEFYTGVLGMTLLTVKKFPEMAFDLYFLAKLTDDERAN
LPENEELAIYTFRQRGILELTHNYGTETQPGRIYHDGNAEPQGFGHICFSVPDLTAAVEWFDKNNVEFKKRPEEGKMKNV
VFIKDVDGYWIEVVQADLMG

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=159, Percent_Identity=47.1698113207547, Blast_Score=157, Evalue=6e-39,
Organism=Escherichia coli, GI1787940, Length=147, Percent_Identity=35.3741496598639, Blast_Score=75, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323639, Length=147, Percent_Identity=47.6190476190476, Blast_Score=138, Evalue=6e-34,
Organism=Drosophila melanogaster, GI20129743, Length=161, Percent_Identity=45.9627329192547, Blast_Score=154, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 20478; Mature: 20346

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00934 GLYOXALASE_I_1 ; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSINYVDQPKGSVVVDGVQPIAAATTGYTFNHTMLRVKDPKASLEFYTGVLGMTLLTVKK
CCCCCCCCCCCCEEEECCCCEEHEECCCEECCEEEEEECCCCCHHHHHHHHHHHHHHHHH
FPEMAFDLYFLAKLTDDERANLPENEELAIYTFRQRGILELTHNYGTETQPGRIYHDGNA
HHHHHHHEEEEEEECCCHHCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCEEEECCCC
EPQGFGHICFSVPDLTAAVEWFDKNNVEFKKRPEEGKMKNVVFIKDVDGYWIEVVQADLM
CCCCCEEEEEECCCHHEEEEECCCCCCCEEECCCCCCCCEEEEEEECCCEEEEEEEHHHC
G
C
>Mature Secondary Structure 
SINYVDQPKGSVVVDGVQPIAAATTGYTFNHTMLRVKDPKASLEFYTGVLGMTLLTVKK
CCCCCCCCCCCEEEECCCCEEHEECCCEECCEEEEEECCCCCHHHHHHHHHHHHHHHHH
FPEMAFDLYFLAKLTDDERANLPENEELAIYTFRQRGILELTHNYGTETQPGRIYHDGNA
HHHHHHHEEEEEEECCCHHCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCEEEECCCC
EPQGFGHICFSVPDLTAAVEWFDKNNVEFKKRPEEGKMKNVVFIKDVDGYWIEVVQADLM
CCCCCEEEEEECCCHHEEEEECCCCCCCEEECCCCCCCCEEEEEEECCCEEEEEEEHHHC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]