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The map label for this gene is cobQ [H]

Identifier: 148643625

GI number: 148643625

Start: 1600799

End: 1602379

Strand: Reverse

Name: cobQ [H]

Synonym: Msm_1565

Alternate gene names: 148643625

Gene position: 1602379-1600799 (Counterclockwise)

Preceding gene: 148643626

Following gene: 148643615

Centisome position: 86.47

GC content: 32.95

Gene sequence:

>1581_bases
ATGACAAATATTTTTAAAGCAAATAAAAAAGTGATTTTAATGGTTAAATGTTTAATGGTTCAGGGAACATCATCAAATGC
AGGAAAAAGTATGCTTGTAGCTGCATTATGTAGAATATATAAAAACAGAGGCTATAACGTAGCTCCTTTTAAATCTCAAA
ATATGTCTTTAAATTCATATACAACTAAAGAAAACGGAGAAATAGGAATAGCTCAAATGTTGCAAGCAGAAGCTGCAATG
GTTGAACCAAGTGTACATATGAACCCTATTCTTTTAAAACCAAAAGGTGATTTTACATCCAATGTAATTATTCAGGGAAA
ATCCATCGGAGATATGAATTTCTACCAGTATCAGCATGACTATCACAACACGGCTTTTGAAGCTATTCAGGAAAGTTTCA
ATATTTTAAAAGAAAAATACGATATTATAATCATTGAAGGAGCAGGATCACCTGCAGAAATTAATATGCGTAAAGAAGAC
CTTGCCAATATGGAAATTGCACATATGGCTGATGCAAATGTTATTTTAATAGCTGATATTGAAATGGGTGGTGTTTTTGC
AGCTATTGCCGGAACATATGTGCTTTTAGATGACTATGACAGATCCAGACTGAAAGCTACTGTCATCAATAAGTTTAGAG
GAAATTTGGATATTTTGAAACCTGGACTTGACAGAATTGAAGAAATCACCGAAGCTCCGGTTTTAGGTGTTCTTCCTTAT
GATGAAACATTAAAACTGCCTGAAGAAGACTCAGCATCCCTAACAACCCACATATTTGATGAAAATAAGGATATTATTAT
TGGAGTTATAAGGCTTCCTAAAATCTCCAATTTTACAGACATTGATCCTTTTGAGTTTGAAGAGGATGTTGGAATTAAAA
TGATTAATCCCAATGATGAAATTGGAAATGTTGATGCTATAATCATTCCCGGAACACGTAATTCAACCAAAGATGCAAAA
GAACTCTTTGACAGCGGACTTGCAAGTAAAATAATAGCCAAATCCGATGAAATCCCAGTTGTTGGAATCTGTGGAGGATA
TCAAATCCTTGGAAATATAATTTATGATGAAAATAAAAAAGAATCTGATGTTGGAACTATTGAAGGGTTAAAATTATTGG
ATATTGAGTCAAAATTCCAGCATACCGAAAAAATTGTAACACAGTCTGAAGCAGTTATACCTCAAAAAGAGGATTTGTCC
GGAATAGCTGGTGAAATATTTGCAAAGATTGCTGGAGAGAAAGTAACCGGTTATGAAATCCATGAAGGTACATCACACCT
GAATTCAAGTAAAGATGTATGCCCATTATTAAATGTTCAAAAAGGACAGGGAAATAATGATGAAGGTGACTTTGACGGAG
CATGTAATGGAAATGTCTTTGGAACATACTTCCATGGAATATTCCACAATTACAATTTCAGACGTGAATTTTTAAATTAT
CTAAGAGTCAAAAAAGGTCTTGAAGCAAAATATGGTGAAGACCCTTACGAAACACAAAAAGATTACTCATTAAATAAATT
AGCACAAATTGTTGAAGAAAATATAGATATGGAAATTATTGACGAACTTCTTTTTTCATAA

Upstream 100 bases:

>100_bases
AAAACTAAATTATTGGAATTTAAACAATGAAAAAGAAAAAAATCACACAAGTTTTTGAAAGAGCCGACATAATTAACCGA
TTAACATTTATAAAATATAA

Downstream 100 bases:

>100_bases
TATAATAAACATAGCTATAATCACCACTATTATTTCCATAAACTTCAATTTCCTTTTTAAAATCTTTTTTAAAGTCATTA
TCATCTAATGTTCGAAGATT

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MTNIFKANKKVILMVKCLMVQGTSSNAGKSMLVAALCRIYKNRGYNVAPFKSQNMSLNSYTTKENGEIGIAQMLQAEAAM
VEPSVHMNPILLKPKGDFTSNVIIQGKSIGDMNFYQYQHDYHNTAFEAIQESFNILKEKYDIIIIEGAGSPAEINMRKED
LANMEIAHMADANVILIADIEMGGVFAAIAGTYVLLDDYDRSRLKATVINKFRGNLDILKPGLDRIEEITEAPVLGVLPY
DETLKLPEEDSASLTTHIFDENKDIIIGVIRLPKISNFTDIDPFEFEEDVGIKMINPNDEIGNVDAIIIPGTRNSTKDAK
ELFDSGLASKIIAKSDEIPVVGICGGYQILGNIIYDENKKESDVGTIEGLKLLDIESKFQHTEKIVTQSEAVIPQKEDLS
GIAGEIFAKIAGEKVTGYEIHEGTSHLNSSKDVCPLLNVQKGQGNNDEGDFDGACNGNVFGTYFHGIFHNYNFRREFLNY
LRVKKGLEAKYGEDPYETQKDYSLNKLAQIVEENIDMEIIDELLFS

Sequences:

>Translated_526_residues
MTNIFKANKKVILMVKCLMVQGTSSNAGKSMLVAALCRIYKNRGYNVAPFKSQNMSLNSYTTKENGEIGIAQMLQAEAAM
VEPSVHMNPILLKPKGDFTSNVIIQGKSIGDMNFYQYQHDYHNTAFEAIQESFNILKEKYDIIIIEGAGSPAEINMRKED
LANMEIAHMADANVILIADIEMGGVFAAIAGTYVLLDDYDRSRLKATVINKFRGNLDILKPGLDRIEEITEAPVLGVLPY
DETLKLPEEDSASLTTHIFDENKDIIIGVIRLPKISNFTDIDPFEFEEDVGIKMINPNDEIGNVDAIIIPGTRNSTKDAK
ELFDSGLASKIIAKSDEIPVVGICGGYQILGNIIYDENKKESDVGTIEGLKLLDIESKFQHTEKIVTQSEAVIPQKEDLS
GIAGEIFAKIAGEKVTGYEIHEGTSHLNSSKDVCPLLNVQKGQGNNDEGDFDGACNGNVFGTYFHGIFHNYNFRREFLNY
LRVKKGLEAKYGEDPYETQKDYSLNKLAQIVEENIDMEIIDELLFS
>Mature_525_residues
TNIFKANKKVILMVKCLMVQGTSSNAGKSMLVAALCRIYKNRGYNVAPFKSQNMSLNSYTTKENGEIGIAQMLQAEAAMV
EPSVHMNPILLKPKGDFTSNVIIQGKSIGDMNFYQYQHDYHNTAFEAIQESFNILKEKYDIIIIEGAGSPAEINMRKEDL
ANMEIAHMADANVILIADIEMGGVFAAIAGTYVLLDDYDRSRLKATVINKFRGNLDILKPGLDRIEEITEAPVLGVLPYD
ETLKLPEEDSASLTTHIFDENKDIIIGVIRLPKISNFTDIDPFEFEEDVGIKMINPNDEIGNVDAIIIPGTRNSTKDAKE
LFDSGLASKIIAKSDEIPVVGICGGYQILGNIIYDENKKESDVGTIEGLKLLDIESKFQHTEKIVTQSEAVIPQKEDLSG
IAGEIFAKIAGEKVTGYEIHEGTSHLNSSKDVCPLLNVQKGQGNNDEGDFDGACNGNVFGTYFHGIFHNYNFRREFLNYL
RVKKGLEAKYGEDPYETQKDYSLNKLAQIVEENIDMEIIDELLFS

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 58444; Mature: 58313

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNIFKANKKVILMVKCLMVQGTSSNAGKSMLVAALCRIYKNRGYNVAPFKSQNMSLNSY
CCCCCCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCE
TTKENGEIGIAQMLQAEAAMVEPSVHMNPILLKPKGDFTSNVIIQGKSIGDMNFYQYQHD
ECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEECCCCCCCCCEEHHHH
YHNTAFEAIQESFNILKEKYDIIIIEGAGSPAEINMRKEDLANMEIAHMADANVILIADI
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEECCCHHHHCCCEEEEECCCCEEEEEEC
EMGGVFAAIAGTYVLLDDYDRSRLKATVINKFRGNLDILKPGLDRIEEITEAPVLGVLPY
CCCCEEEEHHCEEEEEECCCCHHHHHHHHHHHCCCCEEECCCHHHHHHHHCCCEEEEECC
DETLKLPEEDSASLTTHIFDENKDIIIGVIRLPKISNFTDIDPFEFEEDVGIKMINPNDE
CCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCC
IGNVDAIIIPGTRNSTKDAKELFDSGLASKIIAKSDEIPVVGICGGYQILGNIIYDENKK
CCCCCEEEECCCCCCCHHHHHHHHCCHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCC
ESDVGTIEGLKLLDIESKFQHTEKIVTQSEAVIPQKEDLSGIAGEIFAKIAGEKVTGYEI
CCCCCHHCCEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEE
HEGTSHLNSSKDVCPLLNVQKGQGNNDEGDFDGACNGNVFGTYFHGIFHNYNFRREFLNY
CCCHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHCCCHHHHHHHH
LRVKKGLEAKYGEDPYETQKDYSLNKLAQIVEENIDMEIIDELLFS
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure 
TNIFKANKKVILMVKCLMVQGTSSNAGKSMLVAALCRIYKNRGYNVAPFKSQNMSLNSY
CCCCCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCE
TTKENGEIGIAQMLQAEAAMVEPSVHMNPILLKPKGDFTSNVIIQGKSIGDMNFYQYQHD
ECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEECCCCCCCCCEEHHHH
YHNTAFEAIQESFNILKEKYDIIIIEGAGSPAEINMRKEDLANMEIAHMADANVILIADI
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEECCCHHHHCCCEEEEECCCCEEEEEEC
EMGGVFAAIAGTYVLLDDYDRSRLKATVINKFRGNLDILKPGLDRIEEITEAPVLGVLPY
CCCCEEEEHHCEEEEEECCCCHHHHHHHHHHHCCCCEEECCCHHHHHHHHCCCEEEEECC
DETLKLPEEDSASLTTHIFDENKDIIIGVIRLPKISNFTDIDPFEFEEDVGIKMINPNDE
CCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCC
IGNVDAIIIPGTRNSTKDAKELFDSGLASKIIAKSDEIPVVGICGGYQILGNIIYDENKK
CCCCCEEEECCCCCCCHHHHHHHHCCHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCC
ESDVGTIEGLKLLDIESKFQHTEKIVTQSEAVIPQKEDLSGIAGEIFAKIAGEKVTGYEI
CCCCCHHCCEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEE
HEGTSHLNSSKDVCPLLNVQKGQGNNDEGDFDGACNGNVFGTYFHGIFHNYNFRREFLNY
CCCHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHCCCHHHHHHHH
LRVKKGLEAKYGEDPYETQKDYSLNKLAQIVEENIDMEIIDELLFS
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]