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The map label for this gene is pol [H]

Identifier: 148643541

GI number: 148643541

Start: 1506445

End: 1507128

Strand: Reverse

Name: pol [H]

Synonym: Msm_1481

Alternate gene names: 148643541

Gene position: 1507128-1506445 (Counterclockwise)

Preceding gene: 148643547

Following gene: 148643540

Centisome position: 81.33

GC content: 34.06

Gene sequence:

>684_bases
TTGCCAAAAGAAACAAAAGAACAACTGGAATTAGAAGCTGAAATTAAAAATCAGGCTCAAAAATTCATAACCGACCTTAA
TGCAACACTTCCAGAAGTTATGGAACTGGAATATGAAGGATTCTACAGAAGAGGCTTTTTTGTAAGTAAAAAAAGATATG
CAGTTATTGAAGATGGTGAAATCATAGCAAAAGGTCTGGAATTAGTCAGAAGAGACTGGGCACCAATCGTTAAACAAACA
CAAAAAGACGTCCTAAAGGACATCCTAAAAGAAGGAAACACAACCAAAGCAATAAATACAGTTAAAAAAGTTTTAAAAAG
GCTAAAAACTGGAAAAATTGAAGGAAAGGAATTGATAATCCACACACAAATCACAAAACCATTAAGCGAATACAAACAAA
TCGGACCACATGTGGTTGCAGCTAAAAAAATGGAAGAACACGGAATTAAAATAACTAAAGGAACAATTATCCAATATGTG
ATTGTAAAAGGAAAAGGTTCCATCAGCCAGAGAGCAGTTCCTTATGACTACAGTGAAGGAGCAGAATATGATAGAGATTA
CTATATAAACAATCAGATGATTCCGGCTATTGGAAGAATAATGTATTCCTTAGGCTACACAAAACAGGATTTGGAAGACT
TAGCCCAAGGAGAAAAACAAACAAGTTTAGATGCATTTTTCTAA

Upstream 100 bases:

>100_bases
AATAAACAGTATTAACCCGATTAAATATTTCAAAACAATAACAATTTAAATTATCAAAGCAATAATAATAAATAACTAAA
ATAAAATTGGTGTAAAAAAA

Downstream 100 bases:

>100_bases
TAAAACTTTAAATAAGACTAAAATAATATGTAGATATAATGATGCGTAATAACCCTAATGATGACCGTGTTGTATTTTTT
GATGTAGACGATACTTTACT

Product: DNA-dependent DNA polymerase family B protein

Products: NA

Alternate protein names: Vent DNA polymerase; IVPS2; Tli pol-1 intein; IVPS1; Tli pol-2 intein [H]

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MPKETKEQLELEAEIKNQAQKFITDLNATLPEVMELEYEGFYRRGFFVSKKRYAVIEDGEIIAKGLELVRRDWAPIVKQT
QKDVLKDILKEGNTTKAINTVKKVLKRLKTGKIEGKELIIHTQITKPLSEYKQIGPHVVAAKKMEEHGIKITKGTIIQYV
IVKGKGSISQRAVPYDYSEGAEYDRDYYINNQMIPAIGRIMYSLGYTKQDLEDLAQGEKQTSLDAFF

Sequences:

>Translated_227_residues
MPKETKEQLELEAEIKNQAQKFITDLNATLPEVMELEYEGFYRRGFFVSKKRYAVIEDGEIIAKGLELVRRDWAPIVKQT
QKDVLKDILKEGNTTKAINTVKKVLKRLKTGKIEGKELIIHTQITKPLSEYKQIGPHVVAAKKMEEHGIKITKGTIIQYV
IVKGKGSISQRAVPYDYSEGAEYDRDYYINNQMIPAIGRIMYSLGYTKQDLEDLAQGEKQTSLDAFF
>Mature_226_residues
PKETKEQLELEAEIKNQAQKFITDLNATLPEVMELEYEGFYRRGFFVSKKRYAVIEDGEIIAKGLELVRRDWAPIVKQTQ
KDVLKDILKEGNTTKAINTVKKVLKRLKTGKIEGKELIIHTQITKPLSEYKQIGPHVVAAKKMEEHGIKITKGTIIQYVI
VKGKGSISQRAVPYDYSEGAEYDRDYYINNQMIPAIGRIMYSLGYTKQDLEDLAQGEKQTSLDAFF

Specific function: Intein encoded endonucleases are thought to mediate intein mobility by site-specific recombination initiated by endonuclease cleavage at the "homing site" in gene that lack the intein [H]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 DOD-type homing endonuclease domains [H]

Homologues:

Organism=Escherichia coli, GI1786246, Length=190, Percent_Identity=29.4736842105263, Blast_Score=71, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320101, Length=199, Percent_Identity=27.6381909547739, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR004578
- InterPro:   IPR023211
- InterPro:   IPR003586
- InterPro:   IPR003587
- InterPro:   IPR006142
- InterPro:   IPR004042
- InterPro:   IPR006141
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 25992; Mature: 25861

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKETKEQLELEAEIKNQAQKFITDLNATLPEVMELEYEGFYRRGFFVSKKRYAVIEDGE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHCCEEECCCCEEEEECCH
IIAKGLELVRRDWAPIVKQTQKDVLKDILKEGNTTKAINTVKKVLKRLKTGKIEGKELII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
HTQITKPLSEYKQIGPHVVAAKKMEEHGIKITKGTIIQYVIVKGKGSISQRAVPYDYSEG
EEHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCEEEEEEEECCCCCCCCCCCCCCCCC
AEYDRDYYINNQMIPAIGRIMYSLGYTKQDLEDLAQGEKQTSLDAFF
CCCCCCEEECCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHCCHHCCC
>Mature Secondary Structure 
PKETKEQLELEAEIKNQAQKFITDLNATLPEVMELEYEGFYRRGFFVSKKRYAVIEDGE
CCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHCCEEECCCCEEEEECCH
IIAKGLELVRRDWAPIVKQTQKDVLKDILKEGNTTKAINTVKKVLKRLKTGKIEGKELII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
HTQITKPLSEYKQIGPHVVAAKKMEEHGIKITKGTIIQYVIVKGKGSISQRAVPYDYSEG
EEHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCEEEEEEEECCCCCCCCCCCCCCCCC
AEYDRDYYINNQMIPAIGRIMYSLGYTKQDLEDLAQGEKQTSLDAFF
CCCCCCEEECCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1608969; 1475179 [H]