Click here to switch to the map view.

The map label for this gene is glnA [H]

Identifier: 148643478

GI number: 148643478

Start: 1443763

End: 1445136

Strand: Reverse

Name: glnA [H]

Synonym: Msm_1418

Alternate gene names: 148643478

Gene position: 1445136-1443763 (Counterclockwise)

Preceding gene: 148643481

Following gene: 148643477

Centisome position: 77.98

GC content: 37.12

Gene sequence:

>1374_bases
ATGGAGTATGATTTAATGACTGAAAAAGTTAACGAAGAATTAATGGAAAGCGTAATTAAACAAATGAAAGAGGATAATAT
TAAATTTATCCGTTTACAATTTGTTGATATAAATGGAACTGCTAAAAACATTGTAATTCCATTTAAAGAAGATGACATGG
CAGACTTATTTGTTGAAGGTATGTTATTTGACGGATCTTCAATTGCAGGATTCGTAGGAATCAATGAAAGTGACCTTGTT
CTCAAACCTGATATCCACACTTACTCAAGAGTATCCTGGAGGCCTGAAGAATCTGCAGTATGTAGATTTATTTGTGATGT
CTGGACTCCTGACGGAAAACCATTTGTTGGAGACCCAAGAGGTATCCTGAAAAAATCTTTAGCTAAAATTGGAAAAATGG
GATTACAATATAACATAGGTCCTGAACCAGAATTCTTTATTGTAGATATTGACGATGAAGGATACCCAATGCCTTACGAT
GAAGCAGGATACTTTGATGTTGAACCTTTAGATAAAGGACCTGATTTCAGAAGAGAACTAACCTTAAACTTAGAAGAATT
AGATTTCGAAGTAGAAGCTTCTCACCACGAAGTAGGACCTGGTCAAAACGAAATTGCATTTAAATTTAAAGATGCATTAA
AAACTGCAGATGCTGTAATTACCTTTAAACAAGCTATTAAAGCTATTGTAGACAACATGGCTACTTTTGACCAAATGGAT
TACAGAGTAACCTTTATGCCAAAACCGTTCTTTGGTGTAAACGGTAGTGGTATGCACTGTCACCAGTCAGTATTCAAAGG
AGACAGAAACCTGTTCTCTGACCCAAACAGCGAAACCGGATTATCTAAAGACGCACTTCACTTCATGGGAGGATTGCTTG
CTCACGCTCCAGCATTAACCGCAATTACCAACCCTATTGTAAACTCATACAAACGTTTAGTGCCAGGTTATGAAGCTCCA
GTATACAGAGCATACGGTCTTAAAAACAGATCTGCATTAATCAGGGTACCTGCAGCTCGTGGTAAAGCAACCCGTATTGA
ATACAGAGCACCAGACCCAGCATGTAACCCATACTTAGCATTTGCAGTAATGTTAGAAGCAGGTATTGACGGTATTCAAA
ATAAAATTGATCCTGGAGAACCTACCGAAATTAACATCTACTCATTAACCGAAGAAGAAAGAGAAGCTATGGGAATTCAA
GTTTTACCATCCAGTTTATGGGAAGCTTACCATGCACTTGAAGAAGACCCATTGATATTATCCACTTTAGGAGACCACAC
CAGTGAAAAATTCCTAGAGCTCAAATACAAAGAATGGGACGAATACCGTGTACAAGTATTCGGATACGAACAAAACAAAT
ATTTAGATATTTAA

Upstream 100 bases:

>100_bases
TTTATTCAAAACATTTATATAATATGAGAAGGAAATGTATTGCCAAGGGAGGAAGGTATTTTCCGACTAACCTTTTGAAA
CTAATATCCATAAGATTTAT

Downstream 100 bases:

>100_bases
ATCTAAATATACTTTTTTTATTTTTTAAATATAATGATTCTTAAGTAAAATTAGGCATGATTATATTTAAGAAAAAAGGG
AGCATCATATGACTGAATCA

Product: glutamine synthetase, GlnA

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MEYDLMTEKVNEELMESVIKQMKEDNIKFIRLQFVDINGTAKNIVIPFKEDDMADLFVEGMLFDGSSIAGFVGINESDLV
LKPDIHTYSRVSWRPEESAVCRFICDVWTPDGKPFVGDPRGILKKSLAKIGKMGLQYNIGPEPEFFIVDIDDEGYPMPYD
EAGYFDVEPLDKGPDFRRELTLNLEELDFEVEASHHEVGPGQNEIAFKFKDALKTADAVITFKQAIKAIVDNMATFDQMD
YRVTFMPKPFFGVNGSGMHCHQSVFKGDRNLFSDPNSETGLSKDALHFMGGLLAHAPALTAITNPIVNSYKRLVPGYEAP
VYRAYGLKNRSALIRVPAARGKATRIEYRAPDPACNPYLAFAVMLEAGIDGIQNKIDPGEPTEINIYSLTEEEREAMGIQ
VLPSSLWEAYHALEEDPLILSTLGDHTSEKFLELKYKEWDEYRVQVFGYEQNKYLDI

Sequences:

>Translated_457_residues
MEYDLMTEKVNEELMESVIKQMKEDNIKFIRLQFVDINGTAKNIVIPFKEDDMADLFVEGMLFDGSSIAGFVGINESDLV
LKPDIHTYSRVSWRPEESAVCRFICDVWTPDGKPFVGDPRGILKKSLAKIGKMGLQYNIGPEPEFFIVDIDDEGYPMPYD
EAGYFDVEPLDKGPDFRRELTLNLEELDFEVEASHHEVGPGQNEIAFKFKDALKTADAVITFKQAIKAIVDNMATFDQMD
YRVTFMPKPFFGVNGSGMHCHQSVFKGDRNLFSDPNSETGLSKDALHFMGGLLAHAPALTAITNPIVNSYKRLVPGYEAP
VYRAYGLKNRSALIRVPAARGKATRIEYRAPDPACNPYLAFAVMLEAGIDGIQNKIDPGEPTEINIYSLTEEEREAMGIQ
VLPSSLWEAYHALEEDPLILSTLGDHTSEKFLELKYKEWDEYRVQVFGYEQNKYLDI
>Mature_457_residues
MEYDLMTEKVNEELMESVIKQMKEDNIKFIRLQFVDINGTAKNIVIPFKEDDMADLFVEGMLFDGSSIAGFVGINESDLV
LKPDIHTYSRVSWRPEESAVCRFICDVWTPDGKPFVGDPRGILKKSLAKIGKMGLQYNIGPEPEFFIVDIDDEGYPMPYD
EAGYFDVEPLDKGPDFRRELTLNLEELDFEVEASHHEVGPGQNEIAFKFKDALKTADAVITFKQAIKAIVDNMATFDQMD
YRVTFMPKPFFGVNGSGMHCHQSVFKGDRNLFSDPNSETGLSKDALHFMGGLLAHAPALTAITNPIVNSYKRLVPGYEAP
VYRAYGLKNRSALIRVPAARGKATRIEYRAPDPACNPYLAFAVMLEAGIDGIQNKIDPGEPTEINIYSLTEEEREAMGIQ
VLPSSLWEAYHALEEDPLILSTLGDHTSEKFLELKYKEWDEYRVQVFGYEQNKYLDI

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=468, Percent_Identity=25, Blast_Score=122, Evalue=9e-28,
Organism=Escherichia coli, GI1790301, Length=466, Percent_Identity=41.6309012875536, Blast_Score=308, Evalue=3e-85,
Organism=Escherichia coli, GI87081870, Length=384, Percent_Identity=26.8229166666667, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51641; Mature: 51641

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYDLMTEKVNEELMESVIKQMKEDNIKFIRLQFVDINGTAKNIVIPFKEDDMADLFVEG
CCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCEEEEEECCCCHHHHHHHH
MLFDGSSIAGFVGINESDLVLKPDIHTYSRVSWRPEESAVCRFICDVWTPDGKPFVGDPR
EEECCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCH
GILKKSLAKIGKMGLQYNIGPEPEFFIVDIDDEGYPMPYDEAGYFDVEPLDKGPDFRREL
HHHHHHHHHHHHCCEEECCCCCCCEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCHHEE
TLNLEELDFEVEASHHEVGPGQNEIAFKFKDALKTADAVITFKQAIKAIVDNMATFDQMD
EECHHHCCEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
YRVTFMPKPFFGVNGSGMHCHQSVFKGDRNLFSDPNSETGLSKDALHFMGGLLAHAPALT
EEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHH
AITNPIVNSYKRLVPGYEAPVYRAYGLKNRSALIRVPAARGKATRIEYRAPDPACNPYLA
HHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHH
FAVMLEAGIDGIQNKIDPGEPTEINIYSLTEEEREAMGIQVLPSSLWEAYHALEEDPLIL
HHHHHHHCHHHHHHCCCCCCCCEEEEEECCHHHHHHCCEEEEHHHHHHHHHHCCCCCEEE
STLGDHTSEKFLELKYKEWDEYRVQVFGYEQNKYLDI
EECCCCHHHHHHEECCCCCCCEEEEEEEECCCCEECC
>Mature Secondary Structure
MEYDLMTEKVNEELMESVIKQMKEDNIKFIRLQFVDINGTAKNIVIPFKEDDMADLFVEG
CCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCEEEEEECCCCHHHHHHHH
MLFDGSSIAGFVGINESDLVLKPDIHTYSRVSWRPEESAVCRFICDVWTPDGKPFVGDPR
EEECCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCH
GILKKSLAKIGKMGLQYNIGPEPEFFIVDIDDEGYPMPYDEAGYFDVEPLDKGPDFRREL
HHHHHHHHHHHHCCEEECCCCCCCEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCHHEE
TLNLEELDFEVEASHHEVGPGQNEIAFKFKDALKTADAVITFKQAIKAIVDNMATFDQMD
EECHHHCCEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
YRVTFMPKPFFGVNGSGMHCHQSVFKGDRNLFSDPNSETGLSKDALHFMGGLLAHAPALT
EEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHH
AITNPIVNSYKRLVPGYEAPVYRAYGLKNRSALIRVPAARGKATRIEYRAPDPACNPYLA
HHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHH
FAVMLEAGIDGIQNKIDPGEPTEINIYSLTEEEREAMGIQVLPSSLWEAYHALEEDPLIL
HHHHHHHCHHHHHHCCCCCCCCEEEEEECCHHHHHHCCEEEEHHHHHHHHHHCCCCCEEE
STLGDHTSEKFLELKYKEWDEYRVQVFGYEQNKYLDI
EECCCCHHHHHHEECCCCCCCEEEEEEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]