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The map label for this gene is thiC1 [H]

Identifier: 148643448

GI number: 148643448

Start: 1408427

End: 1409725

Strand: Reverse

Name: thiC1 [H]

Synonym: Msm_1388

Alternate gene names: 148643448

Gene position: 1409725-1408427 (Counterclockwise)

Preceding gene: 148643449

Following gene: 148643447

Centisome position: 76.07

GC content: 38.72

Gene sequence:

>1299_bases
ATGACACAGATAATGGATGCAAAAAAAGGTATTTTAACTGATGAAATGAAACAGGTAGCTGCAAAGGAAAATGTTTCTGA
AGAATTCATTTTAAAATCAGTAGCTAACGGAACAATAGTAATTCCATCTAATGTAAACAGAGATATTGAAGCTTCAGGTA
TTGGTGCAGGACTTAGAACAAAAGTAAATGCAACTGTTGGAACCTCAACAGATATTGTTGACTTTGATGAAGAAGTACTA
AAAGCACAAATTGCAATTGATAATGGTGCAGACTGTTTAATGGAATTAAGTATTGGTGGAGATTTAGATGTAATTAGAAG
AAGAGTTCTTGATATGTCTCCATTACCAGTAGGTTCAGTACCAGTTTATCAGGCAGCTATTGAAAGAATTAGAAAGGACG
GCTCCGTAGTTTACATGGAAGAAGAAGATTTATTCAAAACCATTGAAAAACAAGCAAAAGATGGTATTGACTTCATGGCT
GTTCACAGTAGTATCAACATTGAAACTTTAACCAGACTCAAAAGACAGGGTCGTGTAACCGGACTTGTATCCCGTGGAGG
ATCATTTATGTCCGGATGGATTGTTGAAAACGAAAAAGAAAATCCGTTATACTCAAACTTTGACTATGTTTTAGAGATTG
CTAAGGAACATGATGTTGTTCTTTCACTTGCAAACGGTATGAGAGCAGGGTCAATTGCCGATTCAACAGACAGGGCTCAA
ATCCAAGAATTGATCATTTTAGGAGAACTAATCGACAGATCCCGTGAATCTGGAGTGCAATGTATGATTGAAGGACCTGG
ACACATTCCAATTAACGAAATCCCAACAAATGTTATGATTCAAAAGAAAATGTGTTCTAACGCTCCTTTCTATATGCTCG
GACCTATTGTATGTGATGTGGCACCAGGTTACGACCACATCGTATCTGCAATTGGTGCTGCATCTTCTGCAAAAGCAGGA
GCAGATTTCATCTGTTATGTAACTCCTGCAGAACACCTTGCTCTTCCAAACCCTGATGATGTAAAAGAAGGAGTAATTGC
AACAAAAATTGGAGCTTATGCAGGTGACTTGGCAACTGGTGCAGTCGACGGTTCACAGGATTTAGCTATGGCTGAAGCTC
GTAAAAAACTTGACTGGGAAGCACAATACTCATGCGCAATGTTCCCTGAAGCTGCACGTGCAAAAAGAGACCAAAGACCT
CCTGAAGAAGAAGACACATGTACCATGTGTGGAAATTATTGTGCAGTAAAAATCGTAAACGAATGGTTGGACCAGTCCGA
TTCCGACTTAATTAAATAG

Upstream 100 bases:

>100_bases
CTATTACACAAAAATAGAGTAGTATCAACCAAAAATGATAAGTTTTTAAAACTTATTTGATATTTTTAAAAATCATTTGA
CTAAATTAATGTGATGACCT

Downstream 100 bases:

>100_bases
ATAGATTCTATCTATTTTATTCTTTTTTTAAATTATAATTTGCCATTTCCTCAAAACAATTTTTATATCTCTTACAATTT
TATTTAAATAACTTCAATTT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase 1; HMP-P synthase 1; HMP-phosphate synthase 1; HMPP synthase 1; Thiamine biosynthesis protein thiC 1 [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTQIMDAKKGILTDEMKQVAAKENVSEEFILKSVANGTIVIPSNVNRDIEASGIGAGLRTKVNATVGTSTDIVDFDEEVL
KAQIAIDNGADCLMELSIGGDLDVIRRRVLDMSPLPVGSVPVYQAAIERIRKDGSVVYMEEEDLFKTIEKQAKDGIDFMA
VHSSINIETLTRLKRQGRVTGLVSRGGSFMSGWIVENEKENPLYSNFDYVLEIAKEHDVVLSLANGMRAGSIADSTDRAQ
IQELIILGELIDRSRESGVQCMIEGPGHIPINEIPTNVMIQKKMCSNAPFYMLGPIVCDVAPGYDHIVSAIGAASSAKAG
ADFICYVTPAEHLALPNPDDVKEGVIATKIGAYAGDLATGAVDGSQDLAMAEARKKLDWEAQYSCAMFPEAARAKRDQRP
PEEEDTCTMCGNYCAVKIVNEWLDQSDSDLIK

Sequences:

>Translated_432_residues
MTQIMDAKKGILTDEMKQVAAKENVSEEFILKSVANGTIVIPSNVNRDIEASGIGAGLRTKVNATVGTSTDIVDFDEEVL
KAQIAIDNGADCLMELSIGGDLDVIRRRVLDMSPLPVGSVPVYQAAIERIRKDGSVVYMEEEDLFKTIEKQAKDGIDFMA
VHSSINIETLTRLKRQGRVTGLVSRGGSFMSGWIVENEKENPLYSNFDYVLEIAKEHDVVLSLANGMRAGSIADSTDRAQ
IQELIILGELIDRSRESGVQCMIEGPGHIPINEIPTNVMIQKKMCSNAPFYMLGPIVCDVAPGYDHIVSAIGAASSAKAG
ADFICYVTPAEHLALPNPDDVKEGVIATKIGAYAGDLATGAVDGSQDLAMAEARKKLDWEAQYSCAMFPEAARAKRDQRP
PEEEDTCTMCGNYCAVKIVNEWLDQSDSDLIK
>Mature_431_residues
TQIMDAKKGILTDEMKQVAAKENVSEEFILKSVANGTIVIPSNVNRDIEASGIGAGLRTKVNATVGTSTDIVDFDEEVLK
AQIAIDNGADCLMELSIGGDLDVIRRRVLDMSPLPVGSVPVYQAAIERIRKDGSVVYMEEEDLFKTIEKQAKDGIDFMAV
HSSINIETLTRLKRQGRVTGLVSRGGSFMSGWIVENEKENPLYSNFDYVLEIAKEHDVVLSLANGMRAGSIADSTDRAQI
QELIILGELIDRSRESGVQCMIEGPGHIPINEIPTNVMIQKKMCSNAPFYMLGPIVCDVAPGYDHIVSAIGAASSAKAGA
DFICYVTPAEHLALPNPDDVKEGVIATKIGAYAGDLATGAVDGSQDLAMAEARKKLDWEAQYSCAMFPEAARAKRDQRPP
EEEDTCTMCGNYCAVKIVNEWLDQSDSDLIK

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=467, Percent_Identity=39.4004282655246, Blast_Score=325, Evalue=4e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 46885; Mature: 46754

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQIMDAKKGILTDEMKQVAAKENVSEEFILKSVANGTIVIPSNVNRDIEASGIGAGLRT
CCCCCCHHCCCHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEE
KVNATVGTSTDIVDFDEEVLKAQIAIDNGADCLMELSIGGDLDVIRRRVLDMSPLPVGSV
EEEEEECCCCCCCCCCHHHHEEEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCC
PVYQAAIERIRKDGSVVYMEEEDLFKTIEKQAKDGIDFMAVHSSINIETLTRLKRQGRVT
HHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCEE
GLVSRGGSFMSGWIVENEKENPLYSNFDYVLEIAKEHDVVLSLANGMRAGSIADSTDRAQ
EEHHCCCCEEECEEEECCCCCCCCCCHHHHHHHHHHCCEEEEHHCCCCCCCCCCCCHHHH
IQELIILGELIDRSRESGVQCMIEGPGHIPINEIPTNVMIQKKMCSNAPFYMLGPIVCDV
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHCCCCCEEEHHHHCCCCEEEECHHHEEC
APGYDHIVSAIGAASSAKAGADFICYVTPAEHLALPNPDDVKEGVIATKIGAYAGDLATG
CCCHHHHHHHHCCCCCCCCCCCEEEEECCHHHCCCCCCCHHHCCHHHHHHHHHHCCHHHC
AVDGSQDLAMAEARKKLDWEAQYSCAMFPEAARAKRDQRPPEEEDTCTMCGNYCAVKIVN
CCCCCHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHH
EWLDQSDSDLIK
HHHCCCCCCCCC
>Mature Secondary Structure 
TQIMDAKKGILTDEMKQVAAKENVSEEFILKSVANGTIVIPSNVNRDIEASGIGAGLRT
CCCCCHHCCCHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEE
KVNATVGTSTDIVDFDEEVLKAQIAIDNGADCLMELSIGGDLDVIRRRVLDMSPLPVGSV
EEEEEECCCCCCCCCCHHHHEEEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCC
PVYQAAIERIRKDGSVVYMEEEDLFKTIEKQAKDGIDFMAVHSSINIETLTRLKRQGRVT
HHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCEE
GLVSRGGSFMSGWIVENEKENPLYSNFDYVLEIAKEHDVVLSLANGMRAGSIADSTDRAQ
EEHHCCCCEEECEEEECCCCCCCCCCHHHHHHHHHHCCEEEEHHCCCCCCCCCCCCHHHH
IQELIILGELIDRSRESGVQCMIEGPGHIPINEIPTNVMIQKKMCSNAPFYMLGPIVCDV
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHCCCCCEEEHHHHCCCCEEEECHHHEEC
APGYDHIVSAIGAASSAKAGADFICYVTPAEHLALPNPDDVKEGVIATKIGAYAGDLATG
CCCHHHHHHHHCCCCCCCCCCCEEEEECCHHHCCCCCCCHHHCCHHHHHHHHHHCCHHHC
AVDGSQDLAMAEARKKLDWEAQYSCAMFPEAARAKRDQRPPEEEDTCTMCGNYCAVKIVN
CCCCCHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHH
EWLDQSDSDLIK
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]