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The map label for this gene is 148643418

Identifier: 148643418

GI number: 148643418

Start: 1380948

End: 1381952

Strand: Reverse

Name: 148643418

Synonym: Msm_1358

Alternate gene names: NA

Gene position: 1381952-1380948 (Counterclockwise)

Preceding gene: 148643419

Following gene: 148643417

Centisome position: 74.57

GC content: 32.04

Gene sequence:

>1005_bases
ATGTCAATGTATAACATGGACTTAGACAAAGTCATAAGAAAGATTAATAAAAAAGGAGCTAGAACTGTAGGTCTTCAATT
TCCGGAAGGTCTTAAAATGCAGGCAGTTAAAATAGCAAAGGCTATTGAATCACAAACTCCTGCTACCGTGATAATATCTG
GAGACCCTTGTTTTGGAGCCTGTGATGTAAGTGATTACAAAATGAAAGGTTCCGTTGATTTAATCGTTCATTATGGCCAC
ACCCCACTTCCATTAAAATATGAAGTGCCGACACTGTTCATAGAAGCTTTTTCTAATATTGATGTCAAAAAAGACCTTGA
AAAATGTCTTGAAAAACTAGAAGACTATTCAAAAATAGCTCTTGTAACTACAACACAACACTTACATTTGTTAAATGAAA
TTAAAGATTATTTGGAAGACAATGGAAAAGAAGTAGTTCTTGGATCTTCTAAAAATACTAAAAAAGGACAAGTTTTAGGA
TGCAATTTTTCATCAATCAAAAATTTGGACGCTGAAGTTTACTTATTTATAGGTAGCGGAAATTTCCATCCATTAGGAAT
TTATTTATTTACCAAATCACCAGTATTGGCTTTAGACCCATATAACAGTGAAATAAGAGATATCAGTGCTTTTGCAGACA
GAATATTGAGAATCAGATTTGCAAGAATTACAAAAGCAAGAGAAGCTGAAAAATGGGGAATAATAGTTTCTTCTAAAGAG
GGACAATACAGAATGAAACTAGCTAAAGAAATCAAAAAAATTCTAGAAGATAACAAAATGGAGGCATATATCATAATGGC
GGACAATATAAATCCCGACATATTACTGCCATACATGGAATTAGATGCTTTTGTTGTTTCAGCCTGTCCAAGAATAGCTA
TTGATGATTCTCAAATGTATAAAAAACCATTATTAACACCACAGGAACTTGAAATAGTTTTAAATAAAAGACAATGGGAA
AATTACCAATTGGATGAAATTTTATTCCATGAAAGGTACAAATAG

Upstream 100 bases:

>100_bases
AAATTAGTTGAAAAAGAAACTGGCATTCCAATATTTTCAGTTGTAAAATTAGATGTAATTGACGGCAAAGTAGTTATCGA
AAAAACCATTGAAGATTAAA

Downstream 100 bases:

>100_bases
TTTTAAATATTAATAAAAATAAAATTATTATATTAATATTAATAATTCCTTATTTTGGTGATTTGATTATGAGTATAAAA
AACGGGGCATTTGTAATGCC

Product: diphthamide synthase, subunit DPH2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSMYNMDLDKVIRKINKKGARTVGLQFPEGLKMQAVKIAKAIESQTPATVIISGDPCFGACDVSDYKMKGSVDLIVHYGH
TPLPLKYEVPTLFIEAFSNIDVKKDLEKCLEKLEDYSKIALVTTTQHLHLLNEIKDYLEDNGKEVVLGSSKNTKKGQVLG
CNFSSIKNLDAEVYLFIGSGNFHPLGIYLFTKSPVLALDPYNSEIRDISAFADRILRIRFARITKAREAEKWGIIVSSKE
GQYRMKLAKEIKKILEDNKMEAYIIMADNINPDILLPYMELDAFVVSACPRIAIDDSQMYKKPLLTPQELEIVLNKRQWE
NYQLDEILFHERYK

Sequences:

>Translated_334_residues
MSMYNMDLDKVIRKINKKGARTVGLQFPEGLKMQAVKIAKAIESQTPATVIISGDPCFGACDVSDYKMKGSVDLIVHYGH
TPLPLKYEVPTLFIEAFSNIDVKKDLEKCLEKLEDYSKIALVTTTQHLHLLNEIKDYLEDNGKEVVLGSSKNTKKGQVLG
CNFSSIKNLDAEVYLFIGSGNFHPLGIYLFTKSPVLALDPYNSEIRDISAFADRILRIRFARITKAREAEKWGIIVSSKE
GQYRMKLAKEIKKILEDNKMEAYIIMADNINPDILLPYMELDAFVVSACPRIAIDDSQMYKKPLLTPQELEIVLNKRQWE
NYQLDEILFHERYK
>Mature_333_residues
SMYNMDLDKVIRKINKKGARTVGLQFPEGLKMQAVKIAKAIESQTPATVIISGDPCFGACDVSDYKMKGSVDLIVHYGHT
PLPLKYEVPTLFIEAFSNIDVKKDLEKCLEKLEDYSKIALVTTTQHLHLLNEIKDYLEDNGKEVVLGSSKNTKKGQVLGC
NFSSIKNLDAEVYLFIGSGNFHPLGIYLFTKSPVLALDPYNSEIRDISAFADRILRIRFARITKAREAEKWGIIVSSKEG
QYRMKLAKEIKKILEDNKMEAYIIMADNINPDILLPYMELDAFVVSACPRIAIDDSQMYKKPLLTPQELEIVLNKRQWEN
YQLDEILFHERYK

Specific function: Unknown

COG id: COG1736

COG function: function code J; Diphthamide synthase subunit DPH2

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI116686106, Length=333, Percent_Identity=30.3303303303303, Blast_Score=138, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17552166, Length=320, Percent_Identity=29.6875, Blast_Score=140, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6322088, Length=342, Percent_Identity=28.0701754385965, Blast_Score=134, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24662841, Length=337, Percent_Identity=30.8605341246291, Blast_Score=151, Evalue=5e-37,
Organism=Drosophila melanogaster, GI21356957, Length=337, Percent_Identity=30.8605341246291, Blast_Score=151, Evalue=5e-37,
Organism=Drosophila melanogaster, GI21357831, Length=320, Percent_Identity=22.1875, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016435
- InterPro:   IPR002728
- InterPro:   IPR022428 [H]

Pfam domain/function: PF01866 Diphthamide_syn [H]

EC number: NA

Molecular weight: Translated: 38096; Mature: 37965

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMYNMDLDKVIRKINKKGARTVGLQFPEGLKMQAVKIAKAIESQTPATVIISGDPCFGA
CCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEE
CDVSDYKMKGSVDLIVHYGHTPLPLKYEVPTLFIEAFSNIDVKKDLEKCLEKLEDYSKIA
CCCCCEEECCCEEEEEEECCCCCCEEECCHHHEEHHHCCCCHHHHHHHHHHHHHCCCEEE
LVTTTQHLHLLNEIKDYLEDNGKEVVLGSSKNTKKGQVLGCNFSSIKNLDAEVYLFIGSG
EEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCHHHHCCCCCEEEEEEECC
NFHPLGIYLFTKSPVLALDPYNSEIRDISAFADRILRIRFARITKAREAEKWGIIVSSKE
CCCEEEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
GQYRMKLAKEIKKILEDNKMEAYIIMADNINPDILLPYMELDAFVVSACPRIAIDDSQMY
CCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEHHHHHHHHHHCCCCEEECCHHHH
KKPLLTPQELEIVLNKRQWENYQLDEILFHERYK
HCCCCCHHHHHEEEECCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
SMYNMDLDKVIRKINKKGARTVGLQFPEGLKMQAVKIAKAIESQTPATVIISGDPCFGA
CCCCCCHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEE
CDVSDYKMKGSVDLIVHYGHTPLPLKYEVPTLFIEAFSNIDVKKDLEKCLEKLEDYSKIA
CCCCCEEECCCEEEEEEECCCCCCEEECCHHHEEHHHCCCCHHHHHHHHHHHHHCCCEEE
LVTTTQHLHLLNEIKDYLEDNGKEVVLGSSKNTKKGQVLGCNFSSIKNLDAEVYLFIGSG
EEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCHHHHCCCCCEEEEEEECC
NFHPLGIYLFTKSPVLALDPYNSEIRDISAFADRILRIRFARITKAREAEKWGIIVSSKE
CCCEEEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
GQYRMKLAKEIKKILEDNKMEAYIIMADNINPDILLPYMELDAFVVSACPRIAIDDSQMY
CCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEHHHHHHHHHHCCCCEEECCHHHH
KKPLLTPQELEIVLNKRQWENYQLDEILFHERYK
HCCCCCHHHHHEEEECCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]