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The map label for this gene is thrS

Identifier: 148643274

GI number: 148643274

Start: 1214584

End: 1216410

Strand: Reverse

Name: thrS

Synonym: Msm_1214

Alternate gene names: 148643274

Gene position: 1216410-1214584 (Counterclockwise)

Preceding gene: 148643277

Following gene: 148643273

Centisome position: 65.64

GC content: 33.06

Gene sequence:

>1827_bases
ATGAGAATACTACTTATTCATTCTGATTATTTAAATTACAATGTAAAAAATAAAACACCTGTAGCTGAAGAAATCGAAGA
TGCTAAAAAACAAGGAGCATTTGACGAATCTTTAGTTGTATTTACAGCTGTTGAAAAAGACGACGAAAATAACCCACAAG
GGATTGTTAAAAATTTAGTTAAAGAAGTTATTAAAACTAATGACCAAGTTAAAGCAGAAAATATAGTTTTATATCCATAT
GCTCACTTATCTTCTTCTTTAAGCTCTCCAAAAGTAGCTGTTCAAGTATTAAAAGATGCTGAAGAAGCTTTAGATGCTGA
AGGATTAAATGTTAAAAGAGTACCTTTCGGATGGTATAAGGCATTTGAAATTTCCTGTAAAGGACACCCATTAAGTGAAC
TTTCAAGAACCATTACTGCTGAAGAGGAAGAGGAAGAGGAAGTAGAGAAAAAACCTTCTTCATGGTCTATTCTGGATGGA
GATAAAATTATCGATATTGATGATTTCAAATTTGAAAATGATCAGTTGGAAAAACTTGTTAGCTATGAACTTGGTACCGG
CGCATCTGATGCAGGAGAACCACCTCATGTTAAATTAATGAGGGAAAAAGAGTTATGTGACTATGAAAGTGCATCTGATG
TAGGAAACCTCAAATGGTTCCCAAAAGGACGTTTAGTTCGCGATTTACTTGCTGATTATGTTTATAATTTAGTTGTAGAC
CAAGGAGCAATGCCTATTGAAACTCCAATTTTCTATGATTTGGACAACGAAGCTATTAATGTTCATGCAGCTAAATTTGG
AGAAAGACAATACAGAACAGACACCAAGAAAAACTTAATGCTTAGATTTGCATGCTGTTTTGGTGCATTTAGAGTAATGG
CTGATCCATTTATTACCTGGAAAAATTTACCTGCAAAACTCTATGAATTATCCACTTACAGTTTCCGTTTTGAGAAAAAA
GGAGAAGTTGTAGGTCTTAAAAGATTAAGAGCATTTACTATGCCGGATTTCCATAGTTTCTGTGCTGATATGAACAGTAC
CTTAGAAGAATTCTCAAAACAAACTGATATGTGTATTCAGACAGGTGTAGATTTGGATGTTAATTATGAAATAATTTTCA
GAGCTACTAAAGATTTCTATGATGAAAATAAAGATTGGATGTATAGCATCGGTAAAAAAATAGGTAAACCAGTTTTACTT
GAAATTTTACCTGAAAGAAAACATTACTGGTCTTGTAAAATAGACTTTGCAGCTATTGACTACTTAGGAAGACCAATTGA
AAATCCGACTGTTCAAATAGATGTTGAAAGTGGTAAAAGATTTGATATTACATATCTTGGAGAAGATGGAAAAGAACATT
ATCCAACTATTCTTCACTGCAGTCCAACTGGAAGTATAGAAAGGGTTATCTGCAGTTTACTTGAAAAAACAGCTATTGAA
TTAGATGAAAAAGCTCCAATGTTACCTACCTGGTTAAGTCCTATTGAAGTAAGGATTATAACTGTTGGTGAAGATCACAA
AGACTTTGCTAATGAATTATATGATAAAATCAATGCTGAAAATATTCGTGTTGATGTAGATGACAGGGATGAAAGTGTTG
GTAAAAAAATAAGGAATGCTGCTACTGAATGGATTCCTTACATATTTGTTGTTGGAGACAATGAAAAAGAATCTGGTGTG
TTCTCCGTAACAGTTCGTGAAACTGGTGAAAAGGTTGACATGACTGTGGATGAATTAATTAAAGAGATTCTTGACAAAAC
CAAAGGAATGCCTTACAGAGGATTACCTTTACCAAAAGATATCTCAACAAGAATTAATTTCCAATAA

Upstream 100 bases:

>100_bases
ATATTATTTTATGGATTGTTGTTGTATAATTATTTATCGCATAACATGTTTTAAATAATATAATCAATATAATTTAAATT
AATATATTTATGGTGGTTAA

Downstream 100 bases:

>100_bases
ACCTTCTTTTTTGAAGGTTTCCAATCTTTCTTTTTTTGAAAAATAAACTTTTTTTTAGTTATGGATGTGTCTGATTATTA
ATTTTCATAATCTTCGGTAT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 608; Mature: 608

Protein sequence:

>608_residues
MRILLIHSDYLNYNVKNKTPVAEEIEDAKKQGAFDESLVVFTAVEKDDENNPQGIVKNLVKEVIKTNDQVKAENIVLYPY
AHLSSSLSSPKVAVQVLKDAEEALDAEGLNVKRVPFGWYKAFEISCKGHPLSELSRTITAEEEEEEEVEKKPSSWSILDG
DKIIDIDDFKFENDQLEKLVSYELGTGASDAGEPPHVKLMREKELCDYESASDVGNLKWFPKGRLVRDLLADYVYNLVVD
QGAMPIETPIFYDLDNEAINVHAAKFGERQYRTDTKKNLMLRFACCFGAFRVMADPFITWKNLPAKLYELSTYSFRFEKK
GEVVGLKRLRAFTMPDFHSFCADMNSTLEEFSKQTDMCIQTGVDLDVNYEIIFRATKDFYDENKDWMYSIGKKIGKPVLL
EILPERKHYWSCKIDFAAIDYLGRPIENPTVQIDVESGKRFDITYLGEDGKEHYPTILHCSPTGSIERVICSLLEKTAIE
LDEKAPMLPTWLSPIEVRIITVGEDHKDFANELYDKINAENIRVDVDDRDESVGKKIRNAATEWIPYIFVVGDNEKESGV
FSVTVRETGEKVDMTVDELIKEILDKTKGMPYRGLPLPKDISTRINFQ

Sequences:

>Translated_608_residues
MRILLIHSDYLNYNVKNKTPVAEEIEDAKKQGAFDESLVVFTAVEKDDENNPQGIVKNLVKEVIKTNDQVKAENIVLYPY
AHLSSSLSSPKVAVQVLKDAEEALDAEGLNVKRVPFGWYKAFEISCKGHPLSELSRTITAEEEEEEEVEKKPSSWSILDG
DKIIDIDDFKFENDQLEKLVSYELGTGASDAGEPPHVKLMREKELCDYESASDVGNLKWFPKGRLVRDLLADYVYNLVVD
QGAMPIETPIFYDLDNEAINVHAAKFGERQYRTDTKKNLMLRFACCFGAFRVMADPFITWKNLPAKLYELSTYSFRFEKK
GEVVGLKRLRAFTMPDFHSFCADMNSTLEEFSKQTDMCIQTGVDLDVNYEIIFRATKDFYDENKDWMYSIGKKIGKPVLL
EILPERKHYWSCKIDFAAIDYLGRPIENPTVQIDVESGKRFDITYLGEDGKEHYPTILHCSPTGSIERVICSLLEKTAIE
LDEKAPMLPTWLSPIEVRIITVGEDHKDFANELYDKINAENIRVDVDDRDESVGKKIRNAATEWIPYIFVVGDNEKESGV
FSVTVRETGEKVDMTVDELIKEILDKTKGMPYRGLPLPKDISTRINFQ
>Mature_608_residues
MRILLIHSDYLNYNVKNKTPVAEEIEDAKKQGAFDESLVVFTAVEKDDENNPQGIVKNLVKEVIKTNDQVKAENIVLYPY
AHLSSSLSSPKVAVQVLKDAEEALDAEGLNVKRVPFGWYKAFEISCKGHPLSELSRTITAEEEEEEEVEKKPSSWSILDG
DKIIDIDDFKFENDQLEKLVSYELGTGASDAGEPPHVKLMREKELCDYESASDVGNLKWFPKGRLVRDLLADYVYNLVVD
QGAMPIETPIFYDLDNEAINVHAAKFGERQYRTDTKKNLMLRFACCFGAFRVMADPFITWKNLPAKLYELSTYSFRFEKK
GEVVGLKRLRAFTMPDFHSFCADMNSTLEEFSKQTDMCIQTGVDLDVNYEIIFRATKDFYDENKDWMYSIGKKIGKPVLL
EILPERKHYWSCKIDFAAIDYLGRPIENPTVQIDVESGKRFDITYLGEDGKEHYPTILHCSPTGSIERVICSLLEKTAIE
LDEKAPMLPTWLSPIEVRIITVGEDHKDFANELYDKINAENIRVDVDDRDESVGKKIRNAATEWIPYIFVVGDNEKESGV
FSVTVRETGEKVDMTVDELIKEILDKTKGMPYRGLPLPKDISTRINFQ

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=402, Percent_Identity=25.1243781094527, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI38570111, Length=409, Percent_Identity=25.6723716381418, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI20070344, Length=416, Percent_Identity=23.3173076923077, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1788013, Length=320, Percent_Identity=28.4375, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71984184, Length=411, Percent_Identity=25.0608272506083, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI71984192, Length=411, Percent_Identity=25.0608272506083, Blast_Score=132, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6322112, Length=410, Percent_Identity=25.609756097561, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6322654, Length=402, Percent_Identity=24.6268656716418, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24583839, Length=405, Percent_Identity=24.4444444444444, Blast_Score=133, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24583842, Length=405, Percent_Identity=24.4444444444444, Blast_Score=133, Evalue=4e-31,
Organism=Drosophila melanogaster, GI28574141, Length=405, Percent_Identity=24.4444444444444, Blast_Score=133, Evalue=4e-31,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_METS3 (A5UMJ1)

Other databases:

- EMBL:   CP000678
- RefSeq:   YP_001273787.1
- ProteinModelPortal:   A5UMJ1
- SMR:   A5UMJ1
- STRING:   A5UMJ1
- GeneID:   5215704
- GenomeReviews:   CP000678_GR
- KEGG:   msi:Msm_1214
- eggNOG:   arNOG04477
- HOGENOM:   HBG392335
- OMA:   ETPVMYD
- ProtClustDB:   PRK03991
- GO:   GO:0005737
- HAMAP:   MF_00184_A
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR015011
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01047
- ProDom:   PD016189
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF08915 tRNA-Thr_ED; SSF52954 Anticodon_bd

EC number: =6.1.1.3

Molecular weight: Translated: 69451; Mature: 69451

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILLIHSDYLNYNVKNKTPVAEEIEDAKKQGAFDESLVVFTAVEKDDENNPQGIVKNLV
CEEEEEECCCCCEECCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHH
KEVIKTNDQVKAENIVLYPYAHLSSSLSSPKVAVQVLKDAEEALDAEGLNVKRVPFGWYK
HHHHCCCCCEEECCEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEE
AFEISCKGHPLSELSRTITAEEEEEEEVEKKPSSWSILDGDKIIDIDDFKFENDQLEKLV
EEEEEECCCCHHHHHHHHCCCCHHHHHHHCCCCCCEEECCCEEEECCCCCCCHHHHHHHH
SYELGTGASDAGEPPHVKLMREKELCDYESASDVGNLKWFPKGRLVRDLLADYVYNLVVD
HHHCCCCCCCCCCCCCEEEEEHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHC
QGAMPIETPIFYDLDNEAINVHAAKFGERQYRTDTKKNLMLRFACCFGAFRVMADPFITW
CCCCCCCCCEEEECCCCEEEEEHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEE
KNLPAKLYELSTYSFRFEKKGEVVGLKRLRAFTMPDFHSFCADMNSTLEEFSKQTDMCIQ
CCCCHHHEEEEEEEEEECCCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TGVDLDVNYEIIFRATKDFYDENKDWMYSIGKKIGKPVLLEILPERKHYWSCKIDFAAID
CCCCCCCCEEEEEEEEHHHHCCCCHHHHHHHHHHCCCEEEHCCCCCCCEEEEEEEHHHHH
YLGRPIENPTVQIDVESGKRFDITYLGEDGKEHYPTILHCSPTGSIERVICSLLEKTAIE
HCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
LDEKAPMLPTWLSPIEVRIITVGEDHKDFANELYDKINAENIRVDVDDRDESVGKKIRNA
CCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHH
ATEWIPYIFVVGDNEKESGVFSVTVRETGEKVDMTVDELIKEILDKTKGMPYRGLPLPKD
HHCCCCEEEEEECCCCCCCEEEEEEECCCCEECCCHHHHHHHHHHHHCCCCCCCCCCCCC
ISTRINFQ
CCCCCCCC
>Mature Secondary Structure
MRILLIHSDYLNYNVKNKTPVAEEIEDAKKQGAFDESLVVFTAVEKDDENNPQGIVKNLV
CEEEEEECCCCCEECCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHH
KEVIKTNDQVKAENIVLYPYAHLSSSLSSPKVAVQVLKDAEEALDAEGLNVKRVPFGWYK
HHHHCCCCCEEECCEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEE
AFEISCKGHPLSELSRTITAEEEEEEEVEKKPSSWSILDGDKIIDIDDFKFENDQLEKLV
EEEEEECCCCHHHHHHHHCCCCHHHHHHHCCCCCCEEECCCEEEECCCCCCCHHHHHHHH
SYELGTGASDAGEPPHVKLMREKELCDYESASDVGNLKWFPKGRLVRDLLADYVYNLVVD
HHHCCCCCCCCCCCCCEEEEEHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHC
QGAMPIETPIFYDLDNEAINVHAAKFGERQYRTDTKKNLMLRFACCFGAFRVMADPFITW
CCCCCCCCCEEEECCCCEEEEEHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEE
KNLPAKLYELSTYSFRFEKKGEVVGLKRLRAFTMPDFHSFCADMNSTLEEFSKQTDMCIQ
CCCCHHHEEEEEEEEEECCCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TGVDLDVNYEIIFRATKDFYDENKDWMYSIGKKIGKPVLLEILPERKHYWSCKIDFAAID
CCCCCCCCEEEEEEEEHHHHCCCCHHHHHHHHHHCCCEEEHCCCCCCCEEEEEEEHHHHH
YLGRPIENPTVQIDVESGKRFDITYLGEDGKEHYPTILHCSPTGSIERVICSLLEKTAIE
HCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
LDEKAPMLPTWLSPIEVRIITVGEDHKDFANELYDKINAENIRVDVDDRDESVGKKIRNA
CCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHH
ATEWIPYIFVVGDNEKESGVFSVTVRETGEKVDMTVDELIKEILDKTKGMPYRGLPLPKD
HHCCCCEEEEEECCCCCCCEEEEEEECCCCEECCCHHHHHHHHHHHHCCCCCCCCCCCCC
ISTRINFQ
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA