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The map label for this gene is hisH [H]

Identifier: 148643219

GI number: 148643219

Start: 1138244

End: 1138840

Strand: Reverse

Name: hisH [H]

Synonym: Msm_1159

Alternate gene names: 148643219

Gene position: 1138840-1138244 (Counterclockwise)

Preceding gene: 148643220

Following gene: 148643218

Centisome position: 61.45

GC content: 31.66

Gene sequence:

>597_bases
ATGATAACAATTATTGATTATAAAAGCGGAAATTTAAAAAGCATTTCCAATGGATTTAAAAAAATCGGCTGTGATGTTGA
AATTACAAGCAGCATTGAAAAAATAGCTGATAGTGATTATTTGGTTCTTCCGGGAGTTGGAGCTTTTGGCAGTGCAATGG
AAAATCTTGAAGAATTTAAAAAAGTAATTCATGAACATGTAGATGATGATAAACCATTTTTGGGAGTTTGTTTAGGTCAG
CAGGTTCTTATGGACTCAAGTGAAGAAAGTCCTGATGTTGACGGTTTAAAATTATTTGAAGGTCATGCATGCTATCTTCC
AGAAGGACTTAAAATACCTCATATGGGGTGGAATAAATTAGATGTTGTTAAAAAATGTCCAATTTTGGAAGGTGTTGATA
AAGAGTACTTTTATTTTGTTCATTCTTATCATGTTGTTCCGGATAATGATGAAATAATTGCAGGGACCTGTGATTATGGA
ATTGATGTTGTTGCAGCATTAAGTAGGAATAATTTATTTTCAACACAATTTCACCCGGAAAAAAGTGGAGTTGCAGGTTT
AAAGATATTAAAGAATTTTGTTAATTTAGAGTTATAA

Upstream 100 bases:

>100_bases
GCAGAACTTGATGCTCACTCAAAAACTTTAGGAACTGATAAAATTGTTTTTTCTGACTTTGGAGGAATGATTAGGTCAGC
TATTGACTGGAAGTAGAATT

Downstream 100 bases:

>100_bases
TTGGAGATTATATTTATGGATATTGAAGGTTTTGTAAGAAAAAGAATTGATGATTACACATATGAAGATTTGACTGCTCT
TTTGGCAAGTAGAATTAGAG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MITIIDYKSGNLKSISNGFKKIGCDVEITSSIEKIADSDYLVLPGVGAFGSAMENLEEFKKVIHEHVDDDKPFLGVCLGQ
QVLMDSSEESPDVDGLKLFEGHACYLPEGLKIPHMGWNKLDVVKKCPILEGVDKEYFYFVHSYHVVPDNDEIIAGTCDYG
IDVVAALSRNNLFSTQFHPEKSGVAGLKILKNFVNLEL

Sequences:

>Translated_198_residues
MITIIDYKSGNLKSISNGFKKIGCDVEITSSIEKIADSDYLVLPGVGAFGSAMENLEEFKKVIHEHVDDDKPFLGVCLGQ
QVLMDSSEESPDVDGLKLFEGHACYLPEGLKIPHMGWNKLDVVKKCPILEGVDKEYFYFVHSYHVVPDNDEIIAGTCDYG
IDVVAALSRNNLFSTQFHPEKSGVAGLKILKNFVNLEL
>Mature_198_residues
MITIIDYKSGNLKSISNGFKKIGCDVEITSSIEKIADSDYLVLPGVGAFGSAMENLEEFKKVIHEHVDDDKPFLGVCLGQ
QVLMDSSEESPDVDGLKLFEGHACYLPEGLKIPHMGWNKLDVVKKCPILEGVDKEYFYFVHSYHVVPDNDEIIAGTCDYG
IDVVAALSRNNLFSTQFHPEKSGVAGLKILKNFVNLEL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=197, Percent_Identity=37.0558375634518, Blast_Score=129, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6319725, Length=212, Percent_Identity=34.4339622641509, Blast_Score=124, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 21914; Mature: 21914

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIIDYKSGNLKSISNGFKKIGCDVEITSSIEKIADSDYLVLPGVGAFGSAMENLEEFK
CEEEEECCCCCHHHHHHHHHHHCCCEEEHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHH
KVIHEHVDDDKPFLGVCLGQQVLMDSSEESPDVDGLKLFEGHACYLPEGLKIPHMGWNKL
HHHHHHCCCCCCEEHHHHHHHHHHCCCCCCCCCCCEEEECCCEEECCCCCCCCCCCCCHH
DVVKKCPILEGVDKEYFYFVHSYHVVPDNDEIIAGTCDYGIDVVAALSRNNLFSTQFHPE
HHHHHCCCCCCCCCCEEEEEEEEEEECCCCCEEEEECCCCHHHHHHHHCCCEEECCCCCC
KSGVAGLKILKNFVNLEL
CCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITIIDYKSGNLKSISNGFKKIGCDVEITSSIEKIADSDYLVLPGVGAFGSAMENLEEFK
CEEEEECCCCCHHHHHHHHHHHCCCEEEHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHH
KVIHEHVDDDKPFLGVCLGQQVLMDSSEESPDVDGLKLFEGHACYLPEGLKIPHMGWNKL
HHHHHHCCCCCCEEHHHHHHHHHHCCCCCCCCCCCEEEECCCEEECCCCCCCCCCCCCHH
DVVKKCPILEGVDKEYFYFVHSYHVVPDNDEIIAGTCDYGIDVVAALSRNNLFSTQFHPE
HHHHHCCCCCCCCCCEEEEEEEEEEECCCCCEEEEECCCCHHHHHHHHCCCEEECCCCCC
KSGVAGLKILKNFVNLEL
CCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]