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The map label for this gene is pcn

Identifier: 148643197

GI number: 148643197

Start: 1114481

End: 1115215

Strand: Reverse

Name: pcn

Synonym: Msm_1137

Alternate gene names: 148643197

Gene position: 1115215-1114481 (Counterclockwise)

Preceding gene: 148643198

Following gene: 148643196

Centisome position: 60.18

GC content: 32.38

Gene sequence:

>735_bases
ATGTTCAAAGCGGAATTAAGTGATTCTAGTATATTAAAAACCAGTTTTGATGCTATTTCATCTATTGTTGATGAAGTACA
AATCCAAACTGATAGTGAAGGTATGAGGTTAGATGCCTTAGACCGTAGTCATATAACATTCGTACATTTAGAATTAAAAG
CAAGTTTATTTGATGAGTATGTTTGTGATGTTCCTGAAAAGATTAATATCGATACTGGGGAATTCATGAGTGTTCTTAAA
CGTGCAAAATCTCAAGATAGAGTTATCATGTCTTTAGATGAAGGTAATTTCATAATTACTTTTGAAGGGGATGCTACAAG
AACTTTCAAAATAAGATTGATTGATATTGAATATGATAATCCTACTCCTCCAGAACTTGAACATCCTGCATCTTTCAAAG
TTCACTTTGGAATTTTAAAGGATGCAATTAACGATATTGATATTTTCTCAGATAAAATAGCTCTTCAAGTTGATGAAGAT
TACTTTAGAGCATCTGCTGATGGTGAATTTGGTGATGCAAGTGTCAAATACCTTCATGGTGAAAATATCAACACTCAGGA
AAAATCTTTATTCTCACTGGACAAAATTAGGGAAATGCTTAAAGCAGACAAATTCTCTGAAGAAGCTGAAATCGGTTTAG
GTACTGACATGCCATTAAAATTAACTCTCAATATGGTTACTGGTGATGGTAAACTTAGTTTCTTACTTGCTCCAAGATTA
GAATCAGATGAATAA

Upstream 100 bases:

>100_bases
CTGTGGTTTTTTAAATACTGACCTAATGTTTTATAAGTAATAAAGATAAAAATATAATTATCTAATTAATAATTAATAAA
TTCTTATTAGAGGATTATAT

Downstream 100 bases:

>100_bases
TTATTTCATCTATAAACTTTTTTTTATTTTTATATTAAACTCCTCAGAGATGGTATTAAGTGGATCAATTCTTTCAACAA
TTACGTAAAATTCAAAAAAA

Product: DNA polymerase sliding clamp subunit

Products: NA

Alternate protein names: Proliferating cell nuclear antigen homolog; PCNA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MFKAELSDSSILKTSFDAISSIVDEVQIQTDSEGMRLDALDRSHITFVHLELKASLFDEYVCDVPEKINIDTGEFMSVLK
RAKSQDRVIMSLDEGNFIITFEGDATRTFKIRLIDIEYDNPTPPELEHPASFKVHFGILKDAINDIDIFSDKIALQVDED
YFRASADGEFGDASVKYLHGENINTQEKSLFSLDKIREMLKADKFSEEAEIGLGTDMPLKLTLNMVTGDGKLSFLLAPRL
ESDE

Sequences:

>Translated_244_residues
MFKAELSDSSILKTSFDAISSIVDEVQIQTDSEGMRLDALDRSHITFVHLELKASLFDEYVCDVPEKINIDTGEFMSVLK
RAKSQDRVIMSLDEGNFIITFEGDATRTFKIRLIDIEYDNPTPPELEHPASFKVHFGILKDAINDIDIFSDKIALQVDED
YFRASADGEFGDASVKYLHGENINTQEKSLFSLDKIREMLKADKFSEEAEIGLGTDMPLKLTLNMVTGDGKLSFLLAPRL
ESDE
>Mature_244_residues
MFKAELSDSSILKTSFDAISSIVDEVQIQTDSEGMRLDALDRSHITFVHLELKASLFDEYVCDVPEKINIDTGEFMSVLK
RAKSQDRVIMSLDEGNFIITFEGDATRTFKIRLIDIEYDNPTPPELEHPASFKVHFGILKDAINDIDIFSDKIALQVDED
YFRASADGEFGDASVKYLHGENINTQEKSLFSLDKIREMLKADKFSEEAEIGLGTDMPLKLTLNMVTGDGKLSFLLAPRL
ESDE

Specific function: Sliding clamp subunit. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PCNA family

Homologues:

Organism=Homo sapiens, GI33239451, Length=260, Percent_Identity=24.2307692307692, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI4505641, Length=260, Percent_Identity=24.2307692307692, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71995072, Length=227, Percent_Identity=23.7885462555066, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319564, Length=260, Percent_Identity=23.0769230769231, Blast_Score=75, Evalue=8e-15,
Organism=Drosophila melanogaster, GI17136790, Length=260, Percent_Identity=23.0769230769231, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI45552759, Length=260, Percent_Identity=23.0769230769231, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24585303, Length=251, Percent_Identity=23.9043824701195, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCNA_METS3 (A5UMB4)

Other databases:

- EMBL:   CP000678
- RefSeq:   YP_001273710.1
- ProteinModelPortal:   A5UMB4
- STRING:   A5UMB4
- GeneID:   5215723
- GenomeReviews:   CP000678_GR
- KEGG:   msi:Msm_1137
- eggNOG:   arNOG05779
- HOGENOM:   HBG553564
- OMA:   STIRAEP
- ProtClustDB:   PRK01115
- HAMAP:   MF_00317
- InterPro:   IPR000730
- InterPro:   IPR022649
- InterPro:   IPR022659
- InterPro:   IPR022648
- PANTHER:   PTHR11352
- PRINTS:   PR00339
- TIGRFAMs:   TIGR00590

Pfam domain/function: PF02747 PCNA_C; PF00705 PCNA_N

EC number: NA

Molecular weight: Translated: 27473; Mature: 27473

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS01251 PCNA_1; PS00293 PCNA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKAELSDSSILKTSFDAISSIVDEVQIQTDSEGMRLDALDRSHITFVHLELKASLFDEY
CCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCEEEECCCCCEEEEEEEEEHHHHHHH
VCDVPEKINIDTGEFMSVLKRAKSQDRVIMSLDEGNFIITFEGDATRTFKIRLIDIEYDN
HHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEEEECCCCEEEEEEEEEEECCC
PTPPELEHPASFKVHFGILKDAINDIDIFSDKIALQVDEDYFRASADGEFGDASVKYLHG
CCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCEEEEEECHHHHEECCCCCCCCCEEEEEEC
ENINTQEKSLFSLDKIREMLKADKFSEEAEIGLGTDMPLKLTLNMVTGDGKLSFLLAPRL
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEEECCC
ESDE
CCCC
>Mature Secondary Structure
MFKAELSDSSILKTSFDAISSIVDEVQIQTDSEGMRLDALDRSHITFVHLELKASLFDEY
CCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCEEEECCCCCEEEEEEEEEHHHHHHH
VCDVPEKINIDTGEFMSVLKRAKSQDRVIMSLDEGNFIITFEGDATRTFKIRLIDIEYDN
HHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEEEECCCCEEEEEEEEEEECCC
PTPPELEHPASFKVHFGILKDAINDIDIFSDKIALQVDEDYFRASADGEFGDASVKYLHG
CCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCEEEEEECHHHHEECCCCCCCCCEEEEEEC
ENINTQEKSLFSLDKIREMLKADKFSEEAEIGLGTDMPLKLTLNMVTGDGKLSFLLAPRL
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEEECCC
ESDE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA