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The map label for this gene is rnhB

Identifier: 148643039

GI number: 148643039

Start: 939622

End: 940245

Strand: Reverse

Name: rnhB

Synonym: Msm_0979

Alternate gene names: 148643039

Gene position: 940245-939622 (Counterclockwise)

Preceding gene: 148643040

Following gene: 148643038

Centisome position: 50.74

GC content: 33.01

Gene sequence:

>624_bases
ATGGATGTATTGGGAATTGATGAAGCAGGTCGGGGATCTGTTTTAGGACCATTGGTTATTGCAGGGGTTATTGTTCCTGA
GAAAATGGATATTGTTTTAGAAAGAATGGGTGTAAAAGACTCTAAGCGTTTGACTCCTAATAGAAGAACAATTCTGTCCC
GTAAATTAAAAAAAATGTTTGAATATGATTTGGTAGTAATTTCTGCTCAGGATATTGATAATATGAGAGCAGACGGAATC
AACCTTAATGAAATTGAAAGAATTGGAATGGAGAAAATTCTTTCTAATTTAAATCCTGAAAAGGCTATTGTTGATGCAGT
TGATATTAAGGCAGAACGTTTTCAAAACAAACTTGCAAATGACACTGGAGTAAATGTTGTAGCTGAACATAAAGCAGATG
ATAATTATATTGAAGTTAGTGCAGCATCTATTATAGCCAAACAGGAAAGGGATGCACATATAGCTGAAATTAACAAAGAT
TATATTAAAATGGGTGGAATTGGTTCAGGTTATCCTTCCGACCCAATCACTAAAAAATTTTTAACTAATTTTACATATGA
TGAAATGCCTGATTTTGTAAGGAAATCATGGGCAACTGTTGAGAAAATGAAAAATTCTCAATAG

Upstream 100 bases:

>100_bases
TAGCTGCAGAACCTCTTGCATTAGAACCTGAAGTTCGTCTTAGAGCTATGAAATAATTTTTTCAATTATTTCTTTTTTAA
CTTTTTTTGCGCTGATTTTT

Downstream 100 bases:

>100_bases
CTATTTTTTTTAAAAACTATTAAGTATATGGTTAAATAAATTATTTTTATAAAATATTATATTGAGGATAAAAATGATAG
TTGAATATCTGACTCAATTT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MDVLGIDEAGRGSVLGPLVIAGVIVPEKMDIVLERMGVKDSKRLTPNRRTILSRKLKKMFEYDLVVISAQDIDNMRADGI
NLNEIERIGMEKILSNLNPEKAIVDAVDIKAERFQNKLANDTGVNVVAEHKADDNYIEVSAASIIAKQERDAHIAEINKD
YIKMGGIGSGYPSDPITKKFLTNFTYDEMPDFVRKSWATVEKMKNSQ

Sequences:

>Translated_207_residues
MDVLGIDEAGRGSVLGPLVIAGVIVPEKMDIVLERMGVKDSKRLTPNRRTILSRKLKKMFEYDLVVISAQDIDNMRADGI
NLNEIERIGMEKILSNLNPEKAIVDAVDIKAERFQNKLANDTGVNVVAEHKADDNYIEVSAASIIAKQERDAHIAEINKD
YIKMGGIGSGYPSDPITKKFLTNFTYDEMPDFVRKSWATVEKMKNSQ
>Mature_207_residues
MDVLGIDEAGRGSVLGPLVIAGVIVPEKMDIVLERMGVKDSKRLTPNRRTILSRKLKKMFEYDLVVISAQDIDNMRADGI
NLNEIERIGMEKILSNLNPEKAIVDAVDIKAERFQNKLANDTGVNVVAEHKADDNYIEVSAASIIAKQERDAHIAEINKD
YIKMGGIGSGYPSDPITKKFLTNFTYDEMPDFVRKSWATVEKMKNSQ

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Homo sapiens, GI38455391, Length=225, Percent_Identity=34.2222222222222, Blast_Score=87, Evalue=8e-18,
Organism=Escherichia coli, GI1786380, Length=204, Percent_Identity=27.9411764705882, Blast_Score=61, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17536263, Length=218, Percent_Identity=36.2385321100917, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324257, Length=223, Percent_Identity=33.1838565022422, Blast_Score=66, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24580635, Length=218, Percent_Identity=35.3211009174312, Blast_Score=97, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_METS3 (A5ULV6)

Other databases:

- EMBL:   CP000678
- RefSeq:   YP_001273552.1
- ProteinModelPortal:   A5ULV6
- SMR:   A5ULV6
- STRING:   A5ULV6
- GeneID:   5216949
- GenomeReviews:   CP000678_GR
- KEGG:   msi:Msm_0979
- eggNOG:   arNOG07713
- HOGENOM:   HBG584843
- OMA:   AEHKADE
- ProtClustDB:   PRK00015
- GO:   GO:0005737
- HAMAP:   MF_00052_A
- InterPro:   IPR004649
- InterPro:   IPR001352
- InterPro:   IPR020787
- InterPro:   IPR023160
- InterPro:   IPR012337
- Gene3D:   G3DSA:1.10.10.460
- PANTHER:   PTHR10954
- TIGRFAMs:   TIGR00729

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 23126; Mature: 23126

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVLGIDEAGRGSVLGPLVIAGVIVPEKMDIVLERMGVKDSKRLTPNRRTILSRKLKKMF
CCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
EYDLVVISAQDIDNMRADGINLNEIERIGMEKILSNLNPEKAIVDAVDIKAERFQNKLAN
HHCEEEEECCCCCCHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
DTGVNVVAEHKADDNYIEVSAASIIAKQERDAHIAEINKDYIKMGGIGSGYPSDPITKKF
CCCCEEEEECCCCCCEEEEHHHHHHHHHHCCCHHHHHCHHHHHCCCCCCCCCCCHHHHHH
LTNFTYDEMPDFVRKSWATVEKMKNSQ
HHCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDVLGIDEAGRGSVLGPLVIAGVIVPEKMDIVLERMGVKDSKRLTPNRRTILSRKLKKMF
CCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
EYDLVVISAQDIDNMRADGINLNEIERIGMEKILSNLNPEKAIVDAVDIKAERFQNKLAN
HHCEEEEECCCCCCHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
DTGVNVVAEHKADDNYIEVSAASIIAKQERDAHIAEINKDYIKMGGIGSGYPSDPITKKF
CCCCEEEEECCCCCCEEEEHHHHHHHHHHCCCHHHHHCHHHHHCCCCCCCCCCCHHHHHH
LTNFTYDEMPDFVRKSWATVEKMKNSQ
HHCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA