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The map label for this gene is rpoA2 [H]

Identifier: 148642968

GI number: 148642968

Start: 870401

End: 871612

Strand: Reverse

Name: rpoA2 [H]

Synonym: Msm_0908

Alternate gene names: 148642968

Gene position: 871612-870401 (Counterclockwise)

Preceding gene: 148642969

Following gene: 148642967

Centisome position: 47.03

GC content: 33.83

Gene sequence:

>1212_bases
ATGGATGATGCAATAATTAAAAAAGTGGAAGATGCTTTACTTAAAGCTGAAATAAACTTTGATGCAAGAATTGTTGAAAA
TGGAATTATTGACCCATCAAGTCGTGTAAAGTTCCCTGAAAAATATGTGGATGATTTAGCATCTGCTTATGTTAACAGGC
ATTTATCTGATGATGAATTAGATATGCTTATTGGAGAGCTTAAAACTGCCTATGAACGAGCTCATGTTGAAGCTGGTGAG
GCTGTTGGAACAGTAGCTGCACAATCTGTAGGTGAACCGGGTACTCAGATGACTATGCGTACTTTTCACTATGCAGGGGT
AATGGAATTAAACGTAACATTAGGTCTTCCAAGACTTATTGAAATCGTTGACGCAAGAAAAAAAATAGCTACTCCAACAA
TGGATATTTATTTTACTGAAGAAAAACGTCATAATGAAGAATTCGTTAAAACTTTAGCTAATAAAATTGGTAAAAGTACT
GTAAACGATGTTCTCAGTGATTTCAGTTTAAATTATGCTGATATGAAAGTTGAAGCAGTTTTAGACGAAAGAAAAATTGA
AGATAAAAGACTTGATCTTGATGAAATTCTTGCTAAAATTCAGAAAAACTTTAAAAAAGCTGATATTAAGGATAATCTCA
AGATTATTGTCGAACCGCCAAAACGTGAAATTAGGGAACTCCGTCTTTTAGCAGACAAATTCCGTGATTTACAGATAAGT
GGTATTAAAGACATTGGAAAAGTCATTATCCGTAAAGATGAAAGAGAATGGATTATGCACACTGAAGGTTCTAATCTTAA
AGATATTCTCTCTATGGAAGGTATTGACACATACAGAACTACTACAAATGATATTCATGAGATAGAAACTGTATTGGGTA
TTGAAGCAGCTCGTCAATCAATTATTAACGAAGCAAGAAATACTCTTGAAAACCAAGGTCTTAGTGTTGATGTTAGACAC
ATTATGTTAGTTGCAGATATTATGACTTCTGAAGGAGAAGTAAAATCCATTGGTAGACATGGTATTAGTGGTGAAAAATC
AAGCGTTTTAGCTCGTGCAGCTTTTGAAGAAACTGGTAAACATTTACTTCGTGCAAGTATTCGTGGTGAAGTTGATGATT
TAACTGGTATCATCGAAAATATTATTATTGGACAACCAATACCTCTTGGTACCGGTTCAATCGGTGTCAAAATGGCAAAT
AAAAATGAATAG

Upstream 100 bases:

>100_bases
AAGCAGCTGACTTAAATAAAATCATTGATGAAAATAGGTTAGGAGATGCTAATCCAAACTATGATTCAAGTGTTGAACAA
GAGGGTAAATAGGTGTAACT

Downstream 100 bases:

>100_bases
GAGGCTAGATGATGGACGTAGATAGAAGTATCAGGGTAGCTGTCGATACTGGTGATGTGACATTAGGCTCTGAAAAATCA
ATTCAATCTTTAAAATTAGG

Product: DNA-directed RNA polymerase subunit A''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MDDAIIKKVEDALLKAEINFDARIVENGIIDPSSRVKFPEKYVDDLASAYVNRHLSDDELDMLIGELKTAYERAHVEAGE
AVGTVAAQSVGEPGTQMTMRTFHYAGVMELNVTLGLPRLIEIVDARKKIATPTMDIYFTEEKRHNEEFVKTLANKIGKST
VNDVLSDFSLNYADMKVEAVLDERKIEDKRLDLDEILAKIQKNFKKADIKDNLKIIVEPPKREIRELRLLADKFRDLQIS
GIKDIGKVIIRKDEREWIMHTEGSNLKDILSMEGIDTYRTTTNDIHEIETVLGIEAARQSIINEARNTLENQGLSVDVRH
IMLVADIMTSEGEVKSIGRHGISGEKSSVLARAAFEETGKHLLRASIRGEVDDLTGIIENIIIGQPIPLGTGSIGVKMAN
KNE

Sequences:

>Translated_403_residues
MDDAIIKKVEDALLKAEINFDARIVENGIIDPSSRVKFPEKYVDDLASAYVNRHLSDDELDMLIGELKTAYERAHVEAGE
AVGTVAAQSVGEPGTQMTMRTFHYAGVMELNVTLGLPRLIEIVDARKKIATPTMDIYFTEEKRHNEEFVKTLANKIGKST
VNDVLSDFSLNYADMKVEAVLDERKIEDKRLDLDEILAKIQKNFKKADIKDNLKIIVEPPKREIRELRLLADKFRDLQIS
GIKDIGKVIIRKDEREWIMHTEGSNLKDILSMEGIDTYRTTTNDIHEIETVLGIEAARQSIINEARNTLENQGLSVDVRH
IMLVADIMTSEGEVKSIGRHGISGEKSSVLARAAFEETGKHLLRASIRGEVDDLTGIIENIIIGQPIPLGTGSIGVKMAN
KNE
>Mature_403_residues
MDDAIIKKVEDALLKAEINFDARIVENGIIDPSSRVKFPEKYVDDLASAYVNRHLSDDELDMLIGELKTAYERAHVEAGE
AVGTVAAQSVGEPGTQMTMRTFHYAGVMELNVTLGLPRLIEIVDARKKIATPTMDIYFTEEKRHNEEFVKTLANKIGKST
VNDVLSDFSLNYADMKVEAVLDERKIEDKRLDLDEILAKIQKNFKKADIKDNLKIIVEPPKREIRELRLLADKFRDLQIS
GIKDIGKVIIRKDEREWIMHTEGSNLKDILSMEGIDTYRTTTNDIHEIETVLGIEAARQSIINEARNTLENQGLSVDVRH
IMLVADIMTSEGEVKSIGRHGISGEKSSVLARAAFEETGKHLLRASIRGEVDDLTGIIENIIIGQPIPLGTGSIGVKMAN
KNE

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0086

COG function: function code K; DNA-directed RNA polymerase, beta' subunit/160 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta' chain family [H]

Homologues:

Organism=Homo sapiens, GI39725938, Length=357, Percent_Identity=33.6134453781513, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI4505939, Length=411, Percent_Identity=32.6034063260341, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI103471997, Length=152, Percent_Identity=38.8157894736842, Blast_Score=89, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71987878, Length=409, Percent_Identity=32.2738386308068, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI25145495, Length=367, Percent_Identity=31.8801089918256, Blast_Score=194, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI71998295, Length=195, Percent_Identity=31.7948717948718, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324690, Length=359, Percent_Identity=36.2116991643454, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6320061, Length=407, Percent_Identity=29.7297297297297, Blast_Score=191, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6324917, Length=185, Percent_Identity=31.3513513513514, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281360912, Length=360, Percent_Identity=35.2777777777778, Blast_Score=213, Evalue=1e-55,
Organism=Drosophila melanogaster, GI17530899, Length=423, Percent_Identity=31.2056737588652, Blast_Score=210, Evalue=2e-54,
Organism=Drosophila melanogaster, GI17647875, Length=197, Percent_Identity=31.9796954314721, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007081
- InterPro:   IPR012757 [H]

Pfam domain/function: PF04998 RNA_pol_Rpb1_5 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 45087; Mature: 45087

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDAIIKKVEDALLKAEINFDARIVENGIIDPSSRVKFPEKYVDDLASAYVNRHLSDDEL
CCHHHHHHHHHHHHHHCCCCCCEECCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCCCHHH
DMLIGELKTAYERAHVEAGEAVGTVAAQSVGEPGTQMTMRTFHYAGVMELNVTLGLPRLI
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHCEEEEEEEECCHHHH
EIVDARKKIATPTMDIYFTEEKRHNEEFVKTLANKIGKSTVNDVLSDFSLNYADMKVEAV
HHHHHHHHHCCCCEEEEECCHHHCCHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHH
LDERKIEDKRLDLDEILAKIQKNFKKADIKDNLKIIVEPPKREIRELRLLADKFRDLQIS
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCEEC
GIKDIGKVIIRKDEREWIMHTEGSNLKDILSMEGIDTYRTTTNDIHEIETVLGIEAARQS
CHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHH
IINEARNTLENQGLSVDVRHIMLVADIMTSEGEVKSIGRHGISGEKSSVLARAAFEETGK
HHHHHHHHHHHCCCEEHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHH
HLLRASIRGEVDDLTGIIENIIIGQPIPLGTGSIGVKMANKNE
HHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCC
>Mature Secondary Structure
MDDAIIKKVEDALLKAEINFDARIVENGIIDPSSRVKFPEKYVDDLASAYVNRHLSDDEL
CCHHHHHHHHHHHHHHCCCCCCEECCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCCCHHH
DMLIGELKTAYERAHVEAGEAVGTVAAQSVGEPGTQMTMRTFHYAGVMELNVTLGLPRLI
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHCEEEEEEEECCHHHH
EIVDARKKIATPTMDIYFTEEKRHNEEFVKTLANKIGKSTVNDVLSDFSLNYADMKVEAV
HHHHHHHHHCCCCEEEEECCHHHCCHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHH
LDERKIEDKRLDLDEILAKIQKNFKKADIKDNLKIIVEPPKREIRELRLLADKFRDLQIS
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCEEC
GIKDIGKVIIRKDEREWIMHTEGSNLKDILSMEGIDTYRTTTNDIHEIETVLGIEAARQS
CHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHH
IINEARNTLENQGLSVDVRHIMLVADIMTSEGEVKSIGRHGISGEKSSVLARAAFEETGK
HHHHHHHHHHHCCCEEHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHH
HLLRASIRGEVDDLTGIIENIIIGQPIPLGTGSIGVKMANKNE
HHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]