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The map label for this gene is thiI

Identifier: 148642677

GI number: 148642677

Start: 596582

End: 597727

Strand: Reverse

Name: thiI

Synonym: Msm_0617

Alternate gene names: 148642677

Gene position: 597727-596582 (Counterclockwise)

Preceding gene: 148642678

Following gene: 148642676

Centisome position: 32.25

GC content: 36.65

Gene sequence:

>1146_bases
ATGAATTATGATGTAGTTATAGCACGTTATGGTGAAATTGGTTTAAAAAGCTCAAAAGTAAGAGCACGTTTTGAGCGCAA
ATTGGTTAAAAATATTAAAGCAGCTATTGACTGTGAAGTTGACAGAAACCAGGGAAGAATATATATCTTTCCAAAAAACT
ATGGCGAGTGTTTGGAAAATTTAAATAAGGTATTTGGTGTTGTATCTTACTCTCCGGCTGTTTCAACATATGGAAACTAT
GAAGATATTGAAAAAACATTAGGTGAATATGTAGATAATCTTGTTGATGATGGATTCATAGGCAAAGATACAAGATTTGC
CATAAAATGCAGAAGGGTTGGAAACCATGATTTCACATCTCAGGAAATGGCTGCATTCTGCGGTTCTGTAGTTGTTAAAA
AAGTAGGATGTCCTGTTGATTTAACAAATCCTGAACTTAAAATTTATGTTGAAGTAAGAGATGATGAAGCTTTTATTTAT
CATGAAAAAATTGACGGTCCGGGAGGCCTTCCACTGGGAACTCAGGGAAAGGTTGTTGTTTTGGTGTCCAGCGGTATTGA
TTCTCCGGTGGCAGCTTATCTTATGATGAAAAGAGGATGCGAGGTTATAGCACTTCATTGTGATAATGCTCCATTTACAG
GACCTAAAGTTCATGAAAACTTTGATAAAATAATTGATCAGCTTCAAAGTTATGCAAAAGGAGTTCCTATTACAAAAAAA
GTTGTAAAATATGGGGAGTATTTGCAGCAGGCTAAAGACTGTGCTCCGGAAAAAATGACCTGTGTTTTATGCAAGTCAGG
GATGTATAAAATAGCTGAAAAATTAGCACATAAATATGGAGCATCAGCTATTGTAGACGGAAGCAGTGTAGGTCAGGTAG
CTTCACAGACATTGTCTAATATATTGGCTACCCGTCATGGTGTTGACATGCCGATTTTAAGCCCTTTGATAGGTCTGGAC
AAATTGGAAATTACCAGAATAGCTGAAGATATCGGAACTTTTGAAATTTCTAAATTGGATGACGGGGGATGTCATGCAGT
TCCCAAATATCCTGAAACAAAAGCAGATGTTGACAGGGTTGAAGAGGCTTGCAGGGCAATGAATCAAAAAGAAGCTATTG
AAAAAGCCTTTGATTCCATTGATTAA

Upstream 100 bases:

>100_bases
TTTGATATTGTAATGGTTGTTACTTGTATTTCAGTACTGGTACAGGGATCTACTCTTAAATTTATGGCTGGACACCTTAA
AGTATTAAAAGAGGAATAAT

Downstream 100 bases:

>100_bases
TCTTTTTTCTTCTTTTTTTTAAATTCTGATGTAACTTATTTTTTACTTTTCAAAATTATTTGATAAATCTTAAATATATC
CTTATAATATAAATATAAAT

Product: thiamine biosynthesis ATP pyrophosphatase, ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MNYDVVIARYGEIGLKSSKVRARFERKLVKNIKAAIDCEVDRNQGRIYIFPKNYGECLENLNKVFGVVSYSPAVSTYGNY
EDIEKTLGEYVDNLVDDGFIGKDTRFAIKCRRVGNHDFTSQEMAAFCGSVVVKKVGCPVDLTNPELKIYVEVRDDEAFIY
HEKIDGPGGLPLGTQGKVVVLVSSGIDSPVAAYLMMKRGCEVIALHCDNAPFTGPKVHENFDKIIDQLQSYAKGVPITKK
VVKYGEYLQQAKDCAPEKMTCVLCKSGMYKIAEKLAHKYGASAIVDGSSVGQVASQTLSNILATRHGVDMPILSPLIGLD
KLEITRIAEDIGTFEISKLDDGGCHAVPKYPETKADVDRVEEACRAMNQKEAIEKAFDSID

Sequences:

>Translated_381_residues
MNYDVVIARYGEIGLKSSKVRARFERKLVKNIKAAIDCEVDRNQGRIYIFPKNYGECLENLNKVFGVVSYSPAVSTYGNY
EDIEKTLGEYVDNLVDDGFIGKDTRFAIKCRRVGNHDFTSQEMAAFCGSVVVKKVGCPVDLTNPELKIYVEVRDDEAFIY
HEKIDGPGGLPLGTQGKVVVLVSSGIDSPVAAYLMMKRGCEVIALHCDNAPFTGPKVHENFDKIIDQLQSYAKGVPITKK
VVKYGEYLQQAKDCAPEKMTCVLCKSGMYKIAEKLAHKYGASAIVDGSSVGQVASQTLSNILATRHGVDMPILSPLIGLD
KLEITRIAEDIGTFEISKLDDGGCHAVPKYPETKADVDRVEEACRAMNQKEAIEKAFDSID
>Mature_381_residues
MNYDVVIARYGEIGLKSSKVRARFERKLVKNIKAAIDCEVDRNQGRIYIFPKNYGECLENLNKVFGVVSYSPAVSTYGNY
EDIEKTLGEYVDNLVDDGFIGKDTRFAIKCRRVGNHDFTSQEMAAFCGSVVVKKVGCPVDLTNPELKIYVEVRDDEAFIY
HEKIDGPGGLPLGTQGKVVVLVSSGIDSPVAAYLMMKRGCEVIALHCDNAPFTGPKVHENFDKIIDQLQSYAKGVPITKK
VVKYGEYLQQAKDCAPEKMTCVLCKSGMYKIAEKLAHKYGASAIVDGSSVGQVASQTLSNILATRHGVDMPILSPLIGLD
KLEITRIAEDIGTFEISKLDDGGCHAVPKYPETKADVDRVEEACRAMNQKEAIEKAFDSID

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=380, Percent_Identity=29.2105263157895, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_METS3 (A5UKU4)

Other databases:

- EMBL:   CP000678
- RefSeq:   YP_001273190.1
- ProteinModelPortal:   A5UKU4
- SMR:   A5UKU4
- STRING:   A5UKU4
- GeneID:   5217328
- GenomeReviews:   CP000678_GR
- KEGG:   msi:Msm_0617
- eggNOG:   arNOG06091
- HOGENOM:   HBG646072
- OMA:   RREWGRI
- ProtClustDB:   CLSK2392172
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 41961; Mature: 41961

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS51165 THUMP

Important sites: BINDING 265-265 BINDING 287-287 BINDING 296-296

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYDVVIARYGEIGLKSSKVRARFERKLVKNIKAAIDCEVDRNQGRIYIFPKNYGECLEN
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECCHHHHHHH
LNKVFGVVSYSPAVSTYGNYEDIEKTLGEYVDNLVDDGFIGKDTRFAIKCRRVGNHDFTS
HHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCH
QEMAAFCGSVVVKKVGCPVDLTNPELKIYVEVRDDEAFIYHEKIDGPGGLPLGTQGKVVV
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCEEE
LVSSGIDSPVAAYLMMKRGCEVIALHCDNAPFTGPKVHENFDKIIDQLQSYAKGVPITKK
EEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
VVKYGEYLQQAKDCAPEKMTCVLCKSGMYKIAEKLAHKYGASAIVDGSSVGQVASQTLSN
HHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHH
ILATRHGVDMPILSPLIGLDKLEITRIAEDIGTFEISKLDDGGCHAVPKYPETKADVDRV
HHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHH
EEACRAMNQKEAIEKAFDSID
HHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNYDVVIARYGEIGLKSSKVRARFERKLVKNIKAAIDCEVDRNQGRIYIFPKNYGECLEN
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECCHHHHHHH
LNKVFGVVSYSPAVSTYGNYEDIEKTLGEYVDNLVDDGFIGKDTRFAIKCRRVGNHDFTS
HHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCH
QEMAAFCGSVVVKKVGCPVDLTNPELKIYVEVRDDEAFIYHEKIDGPGGLPLGTQGKVVV
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCEEE
LVSSGIDSPVAAYLMMKRGCEVIALHCDNAPFTGPKVHENFDKIIDQLQSYAKGVPITKK
EEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
VVKYGEYLQQAKDCAPEKMTCVLCKSGMYKIAEKLAHKYGASAIVDGSSVGQVASQTLSN
HHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHH
ILATRHGVDMPILSPLIGLDKLEITRIAEDIGTFEISKLDDGGCHAVPKYPETKADVDRV
HHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHH
EEACRAMNQKEAIEKAFDSID
HHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA