Click here to switch to the map view.

The map label for this gene is purP

Identifier: 148642566

GI number: 148642566

Start: 478161

End: 479252

Strand: Reverse

Name: purP

Synonym: Msm_0506

Alternate gene names: 148642566

Gene position: 479252-478161 (Counterclockwise)

Preceding gene: 148642569

Following gene: 148642561

Centisome position: 25.86

GC content: 34.52

Gene sequence:

>1092_bases
ATGGGAGAAGTTAAACTTGAAGATATTTTTGAGATATTAGACAATTACGACAAAGACAATATAACCATAGCTACTCTTGG
AAGCCACACTTCCTTGCATATTCTACAAGGTGCAAAAGAAGAAGGTTTTAGAACAGCTATTGTTTGTGAAAAAGGAAGAG
AAGTACCATATCAACGTTTTGATGTTGCAGATGAATATATAATCGTTGACAAATTTAAAGACATTGTCAATGAGGATGTT
CAGCAAAAGCTAAGAGACATGAATGCTATTGTTATACCTCACGGTTCATTTGTAGCATATGCAGGTCTTGACAATGTTGA
AGATAAATTTAATGTTCCTATGTTTGGTAATAGGGATATTTTAAGATGGGAAGCTGAAAGAGACAAAGAAAGAGCACTTC
TTGTTGAAGGTGAAGTAAGAATACCTTACAAATATGATAATCCTTCAGAAATTGACCGTCCGGTTATGGTTAAATTCCCT
GGTGCAAGGGGAGGAAGAGGTTATTTTGTAGCATCATCTCCTGAAGAATTCAATAAAAAAATAGATGCCATGAAAGCTCG
CGGATGGCTGGAAGACTCAGATGTAGCTAATGCACATATTGAAGAATATGTTTCCGGATGTAATTACTGTATACATTATT
TCTATTCCGCTTTGGAAGATGAAGTTGAATTGTTAGGTATGGATACAAGATATGAATCCAGTATTGACGGTTTTGTAAGA
ATGCCTGCTAAAGACCAGTTAGATATTGATTTAAGCCCATCTTATGTGGTTACAGGTAATCATCCTGCAGTTATCAGAGA
ATCTTTACTTCCTCAAGTATTTGAAATGGCGGATAAATTAGTGGAAAGTGCTAAAAAACTTGTTGCTCCTGGATTAAACG
GCCCGTTCTGTATGCAAACTTTAGTAAATGATAATTTAGAAGTAATCTGTTTTGAAATAAGTGCTAGAACCGATGGTGGA
ACTAATACTTTTATGGGAGGTTCACCTTACAGCTATTTAACTTATGGAAAACCTATGAGTATGGGTAGAAGAATTGCCCT
TGAAATTAAAAATGCAATTAAAAAAGAAGAATTGGAAAAAATAATAACTTAA

Upstream 100 bases:

>100_bases
AAAATTAACTGTTCTGTTCAATCTTTTTTTTAAAAACAAAAAGGGTTATAATATCTTAAATATATATTATTATACATCAA
TTCTTTGAGGAGATATTAAA

Downstream 100 bases:

>100_bases
AATTATTTTTTTCCTTTTTTATTTTTACCTGTTTTTGCTTGTTTTTCCTTTGCAGCAATTGCTCTTTGCCTATTTCTGTA
AGTGTATGCTCCAACAAAAC

Product: 5-formaminoimidazole-4-carboxamide-1-(beta)-D- ribofuranosyl 5'-monophosphate synthetase

Products: NA

Alternate protein names: 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligase

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MGEVKLEDIFEILDNYDKDNITIATLGSHTSLHILQGAKEEGFRTAIVCEKGREVPYQRFDVADEYIIVDKFKDIVNEDV
QQKLRDMNAIVIPHGSFVAYAGLDNVEDKFNVPMFGNRDILRWEAERDKERALLVEGEVRIPYKYDNPSEIDRPVMVKFP
GARGGRGYFVASSPEEFNKKIDAMKARGWLEDSDVANAHIEEYVSGCNYCIHYFYSALEDEVELLGMDTRYESSIDGFVR
MPAKDQLDIDLSPSYVVTGNHPAVIRESLLPQVFEMADKLVESAKKLVAPGLNGPFCMQTLVNDNLEVICFEISARTDGG
TNTFMGGSPYSYLTYGKPMSMGRRIALEIKNAIKKEELEKIIT

Sequences:

>Translated_363_residues
MGEVKLEDIFEILDNYDKDNITIATLGSHTSLHILQGAKEEGFRTAIVCEKGREVPYQRFDVADEYIIVDKFKDIVNEDV
QQKLRDMNAIVIPHGSFVAYAGLDNVEDKFNVPMFGNRDILRWEAERDKERALLVEGEVRIPYKYDNPSEIDRPVMVKFP
GARGGRGYFVASSPEEFNKKIDAMKARGWLEDSDVANAHIEEYVSGCNYCIHYFYSALEDEVELLGMDTRYESSIDGFVR
MPAKDQLDIDLSPSYVVTGNHPAVIRESLLPQVFEMADKLVESAKKLVAPGLNGPFCMQTLVNDNLEVICFEISARTDGG
TNTFMGGSPYSYLTYGKPMSMGRRIALEIKNAIKKEELEKIIT
>Mature_362_residues
GEVKLEDIFEILDNYDKDNITIATLGSHTSLHILQGAKEEGFRTAIVCEKGREVPYQRFDVADEYIIVDKFKDIVNEDVQ
QKLRDMNAIVIPHGSFVAYAGLDNVEDKFNVPMFGNRDILRWEAERDKERALLVEGEVRIPYKYDNPSEIDRPVMVKFPG
ARGGRGYFVASSPEEFNKKIDAMKARGWLEDSDVANAHIEEYVSGCNYCIHYFYSALEDEVELLGMDTRYESSIDGFVRM
PAKDQLDIDLSPSYVVTGNHPAVIRESLLPQVFEMADKLVESAKKLVAPGLNGPFCMQTLVNDNLEVICFEISARTDGGT
NTFMGGSPYSYLTYGKPMSMGRRIALEIKNAIKKEELEKIIT

Specific function: Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'- monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1- beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates

COG id: COG1759

COG function: function code R; ATP-utilizing enzymes of ATP-grasp superfamily (probably carboligases)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURP_METS3 (A5UKI3)

Other databases:

- EMBL:   CP000678
- RefSeq:   YP_001273079.1
- ProteinModelPortal:   A5UKI3
- SMR:   A5UKI3
- STRING:   A5UKI3
- GeneID:   5216345
- GenomeReviews:   CP000678_GR
- KEGG:   msi:Msm_0506
- eggNOG:   arNOG04185
- HOGENOM:   HBG392202
- OMA:   MGQRIAR
- ProtClustDB:   PRK13278
- HAMAP:   MF_01163
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR009720
- InterPro:   IPR010672
- InterPro:   IPR013817
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20

Pfam domain/function: PF06849 DUF1246; PF06973 DUF1297

EC number: NA

Molecular weight: Translated: 41018; Mature: 40887

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS50975 ATP_GRASP; PS00603 TK_CELLULAR_TYPE

Important sites: BINDING 29-29 BINDING 96-96 BINDING 232-232 BINDING 260-260

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEVKLEDIFEILDNYDKDNITIATLGSHTSLHILQGAKEEGFRTAIVCEKGREVPYQRF
CCCCCHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCEEEEEECCCCCCCCHHH
DVADEYIIVDKFKDIVNEDVQQKLRDMNAIVIPHGSFVAYAGLDNVEDKFNVPMFGNRDI
CCCCCEEEHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCCCCCCHHCCCCCCCCCCE
LRWEAERDKERALLVEGEVRIPYKYDNPSEIDRPVMVKFPGARGGRGYFVASSPEEFNKK
EEEECCCCCCCEEEEECCEEEEEECCCHHHCCCCEEEEECCCCCCCEEEEECCHHHHHHH
IDAMKARGWLEDSDVANAHIEEYVSGCNYCIHYFYSALEDEVELLGMDTRYESSIDGFVR
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
MPAKDQLDIDLSPSYVVTGNHPAVIRESLLPQVFEMADKLVESAKKLVAPGLNGPFCMQT
CCCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LVNDNLEVICFEISARTDGGTNTFMGGSPYSYLTYGKPMSMGRRIALEIKNAIKKEELEK
HHCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEECCCCHHCCCEEHHHHHHHHHHHHHHH
IIT
HCC
>Mature Secondary Structure 
GEVKLEDIFEILDNYDKDNITIATLGSHTSLHILQGAKEEGFRTAIVCEKGREVPYQRF
CCCCHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCEEEEEECCCCCCCCHHH
DVADEYIIVDKFKDIVNEDVQQKLRDMNAIVIPHGSFVAYAGLDNVEDKFNVPMFGNRDI
CCCCCEEEHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCCCCCCHHCCCCCCCCCCE
LRWEAERDKERALLVEGEVRIPYKYDNPSEIDRPVMVKFPGARGGRGYFVASSPEEFNKK
EEEECCCCCCCEEEEECCEEEEEECCCHHHCCCCEEEEECCCCCCCEEEEECCHHHHHHH
IDAMKARGWLEDSDVANAHIEEYVSGCNYCIHYFYSALEDEVELLGMDTRYESSIDGFVR
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
MPAKDQLDIDLSPSYVVTGNHPAVIRESLLPQVFEMADKLVESAKKLVAPGLNGPFCMQT
CCCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LVNDNLEVICFEISARTDGGTNTFMGGSPYSYLTYGKPMSMGRRIALEIKNAIKKEELEK
HHCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEECCCCHHCCCEEHHHHHHHHHHHHHHH
IIT
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA