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The map label for this gene is gidA

Identifier: 148381579

GI number: 148381579

Start: 3880989

End: 3882866

Strand: Reverse

Name: gidA

Synonym: CBO3642

Alternate gene names: 148381579

Gene position: 3882866-3880989 (Counterclockwise)

Preceding gene: 148381580

Following gene: 148381578

Centisome position: 99.9

GC content: 29.82

Gene sequence:

>1878_bases
ATGAAATATTCAGCTGGAGATTTTGATGTAGTAGTTATTGGAGCAGGTCATGCGGGATGCGAGGCTGCATTAGCTTCTGC
AAGAATGGGATGTAAAACATTAATATGTACTATGAACCTAGATAGTATTGCTCTTATGGCTTGTAATCCTAATATAGGTG
GAACAGCAAAAGGACATTTAGTAAGAGAAATAGATGCTTTAGGTGGAGAAATGGGAATAAATATAGACCATACATTTATA
CAATCTAGAATGCTTAATACTTCTAAGGGTCCAGCAGTTCATTCTTTAAGAGCTCAAGCAGATAAAAAAAGATATAGTGA
GAGAATGAAACATCTACTAGAGAAGGAAGATAATGTTGTTTTAAGACAGCTAGAAGTTATAGAAATAGATGTAGAGGATA
ATGAAGTTAAAGGTGTTTTAACTAAAAATGGAGCATATTTTACTACAAAAGCTATAATATTATGTACTGGAACTTATTTA
AAGGGTAAAATAATAATAGGAGATATAATATATAGTAGTGGACCAAGTGGTTTGTATCCTGCTAATGATTTATCTCAAAG
TTTATTAGATTTGGGTATAAATCTTAGAAGATTTAAAACAGGAACCCCTGCAAGAATAAATAAAAGATCAGTAGATTTTT
CTAAAATGATAGAACAACCAGGAGACGAAAAAATAGTTCCTTTTTCATTTATTCATAATAAACTAGACAAAGACCAGATT
TCTTGTTATTTAACTTATACATCTGAAGAAACTCATAAAATAATACATGAAAATATACATAGATCTCCATTATATAATGG
ATCAATAGAAGGGGTAGGTCCAAGATACTGCCCATCTATTGAAGATAAGATTGTTAGATTTCCAGATAAAGATAAGCATC
AAATATTCATAGAACCAGAAGGTGAAAATACAGAAGAATTATATGTTGGCGGTATGTCTAGTTCATTACCAGAAGATGTT
CAAATAAAAATGTATAGAAGTGTACCAGGTTTAGAAAATGCAGAAATACTAAGAACTGCCTATGCTATAGAGTATGATTG
TATTGATCCTCAACAACTAGATTTAACCTTAGAATTCAAAAATATAAATGGGTTATATGGGGCAGGTCAATTTAATGGAA
GTTCAGGTTATGAGGAAGCAGCAGCACAGGGGCTTATAGCTGGAATTAATGCTGTGTTAAAAATAAAAGAAAAAAACCCA
TTAATATTGAAAAGATCAGATGCTTATATAGGTGTACTTATAGATGATTTAGTAACAAAGGGCACAAATGAGCCTTATAG
AATGATGACATCAAGAGCAGAATATAGATTGCTGTTAAGACAAGATAATGCAGATTTAAGATTAACAGAGTTAGGCTATA
AAGTCGGACTTGTAAAAGAGGATAGGTATAATAAATTTCTAAATAGGAAAAAAAATGTAGAAAATGAAATAGAAAGATTA
AGAAATATGCAAATAACAGGAAAAAGAGAAATAAACGAATTTTTATTAGAGAAAGGGTCTACTGAACTAAAAAAGCCTAT
TAGTTTATATGAATTAATAAAAAGACCAGAATTAGATTATTTTAAAGTTGAATCTTTAGATGATAAAAGACCAAGTTTAA
GTGATGATGAAAAAGAAGAAATAAATATAATTGCAAAATATGAGGGATATATAAACAAACAGTTAGAACAAGTTGAGCAA
TTTAAGAAGTATGAAGATAGATTAATACCGAAAAGTATTAATTATCTAGATATAAAAGGGTTGAGATTAGAGGCTATTCA
AAAATTGGAAAAAATAAAGCCTATAAATATAGGGCAGGCTTCTAGAATTTCAGGTGTATCTCCAGCAGATATATCAGTAT
TACTTATATATATGGAAAGAAAAAATAGAGAAAACTAG

Upstream 100 bases:

>100_bases
ATGCGTGGAAATATCTTGGAGAAATTACTGGTGATACTTTAGATGAGAACATTATAGATAAAATATTTTCTGAATTTTGT
TTAGGAAAGTAGGTAATTAT

Downstream 100 bases:

>100_bases
AAAATCCTTTTGGAGGTTTTTAAATGGAATTTTTCAACATACTACAATCAGCATGCAATGATGTTAATTTAGATTTTAAT
GATAAAAAATATAATCAGCT

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MKYSAGDFDVVVIGAGHAGCEAALASARMGCKTLICTMNLDSIALMACNPNIGGTAKGHLVREIDALGGEMGINIDHTFI
QSRMLNTSKGPAVHSLRAQADKKRYSERMKHLLEKEDNVVLRQLEVIEIDVEDNEVKGVLTKNGAYFTTKAIILCTGTYL
KGKIIIGDIIYSSGPSGLYPANDLSQSLLDLGINLRRFKTGTPARINKRSVDFSKMIEQPGDEKIVPFSFIHNKLDKDQI
SCYLTYTSEETHKIIHENIHRSPLYNGSIEGVGPRYCPSIEDKIVRFPDKDKHQIFIEPEGENTEELYVGGMSSSLPEDV
QIKMYRSVPGLENAEILRTAYAIEYDCIDPQQLDLTLEFKNINGLYGAGQFNGSSGYEEAAAQGLIAGINAVLKIKEKNP
LILKRSDAYIGVLIDDLVTKGTNEPYRMMTSRAEYRLLLRQDNADLRLTELGYKVGLVKEDRYNKFLNRKKNVENEIERL
RNMQITGKREINEFLLEKGSTELKKPISLYELIKRPELDYFKVESLDDKRPSLSDDEKEEINIIAKYEGYINKQLEQVEQ
FKKYEDRLIPKSINYLDIKGLRLEAIQKLEKIKPINIGQASRISGVSPADISVLLIYMERKNREN

Sequences:

>Translated_625_residues
MKYSAGDFDVVVIGAGHAGCEAALASARMGCKTLICTMNLDSIALMACNPNIGGTAKGHLVREIDALGGEMGINIDHTFI
QSRMLNTSKGPAVHSLRAQADKKRYSERMKHLLEKEDNVVLRQLEVIEIDVEDNEVKGVLTKNGAYFTTKAIILCTGTYL
KGKIIIGDIIYSSGPSGLYPANDLSQSLLDLGINLRRFKTGTPARINKRSVDFSKMIEQPGDEKIVPFSFIHNKLDKDQI
SCYLTYTSEETHKIIHENIHRSPLYNGSIEGVGPRYCPSIEDKIVRFPDKDKHQIFIEPEGENTEELYVGGMSSSLPEDV
QIKMYRSVPGLENAEILRTAYAIEYDCIDPQQLDLTLEFKNINGLYGAGQFNGSSGYEEAAAQGLIAGINAVLKIKEKNP
LILKRSDAYIGVLIDDLVTKGTNEPYRMMTSRAEYRLLLRQDNADLRLTELGYKVGLVKEDRYNKFLNRKKNVENEIERL
RNMQITGKREINEFLLEKGSTELKKPISLYELIKRPELDYFKVESLDDKRPSLSDDEKEEINIIAKYEGYINKQLEQVEQ
FKKYEDRLIPKSINYLDIKGLRLEAIQKLEKIKPINIGQASRISGVSPADISVLLIYMERKNREN
>Mature_625_residues
MKYSAGDFDVVVIGAGHAGCEAALASARMGCKTLICTMNLDSIALMACNPNIGGTAKGHLVREIDALGGEMGINIDHTFI
QSRMLNTSKGPAVHSLRAQADKKRYSERMKHLLEKEDNVVLRQLEVIEIDVEDNEVKGVLTKNGAYFTTKAIILCTGTYL
KGKIIIGDIIYSSGPSGLYPANDLSQSLLDLGINLRRFKTGTPARINKRSVDFSKMIEQPGDEKIVPFSFIHNKLDKDQI
SCYLTYTSEETHKIIHENIHRSPLYNGSIEGVGPRYCPSIEDKIVRFPDKDKHQIFIEPEGENTEELYVGGMSSSLPEDV
QIKMYRSVPGLENAEILRTAYAIEYDCIDPQQLDLTLEFKNINGLYGAGQFNGSSGYEEAAAQGLIAGINAVLKIKEKNP
LILKRSDAYIGVLIDDLVTKGTNEPYRMMTSRAEYRLLLRQDNADLRLTELGYKVGLVKEDRYNKFLNRKKNVENEIERL
RNMQITGKREINEFLLEKGSTELKKPISLYELIKRPELDYFKVESLDDKRPSLSDDEKEEINIIAKYEGYINKQLEQVEQ
FKKYEDRLIPKSINYLDIKGLRLEAIQKLEKIKPINIGQASRISGVSPADISVLLIYMERKNREN

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=641, Percent_Identity=40.2496099843994, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI19882217, Length=666, Percent_Identity=38.7387387387387, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI183227703, Length=681, Percent_Identity=37.8854625550661, Blast_Score=419, Evalue=1e-117,
Organism=Escherichia coli, GI2367273, Length=616, Percent_Identity=49.6753246753247, Blast_Score=612, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI17534255, Length=620, Percent_Identity=39.8387096774194, Blast_Score=457, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6321202, Length=620, Percent_Identity=42.9032258064516, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24658174, Length=639, Percent_Identity=41.9405320813771, Blast_Score=445, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_CLOB1 (A7FPL9)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385956.1
- ProteinModelPortal:   A7FPL9
- SMR:   A7FPL9
- STRING:   A7FPL9
- GeneID:   5397610
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3735
- eggNOG:   COG0445
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   CBOT441770:CLB_3735-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 70538; Mature: 70538

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYSAGDFDVVVIGAGHAGCEAALASARMGCKTLICTMNLDSIALMACNPNIGGTAKGHL
CCCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCCCCCCHH
VREIDALGGEMGINIDHTFIQSRMLNTSKGPAVHSLRAQADKKRYSERMKHLLEKEDNVV
HHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEE
LRQLEVIEIDVEDNEVKGVLTKNGAYFTTKAIILCTGTYLKGKIIIGDIIYSSGPSGLYP
EEEEEEEEEEECCCCEEEEEECCCCEEEEEEEEEEECCEEECEEEEEEEEECCCCCCCCC
ANDLSQSLLDLGINLRRFKTGTPARINKRSVDFSKMIEQPGDEKIVPFSFIHNKLDKDQI
CHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHCCCCCE
SCYLTYTSEETHKIIHENIHRSPLYNGSIEGVGPRYCPSIEDKIVRFPDKDKHQIFIEPE
EEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCCCCEEEEECC
GENTEELYVGGMSSSLPEDVQIKMYRSVPGLENAEILRTAYAIEYDCIDPQQLDLTLEFK
CCCCCEEEEECCCCCCCCCEEEEEEECCCCCCCHHHHHHEEEEEEECCCCCEEEEEEEEE
NINGLYGAGQFNGSSGYEEAAAQGLIAGINAVLKIKEKNPLILKRSDAYIGVLIDDLVTK
CCCCEEECCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCEEEEEHHHHHHC
GTNEPYRMMTSRAEYRLLLRQDNADLRLTELGYKVGLVKEDRYNKFLNRKKNVENEIERL
CCCCHHHHHHCCCCEEEEEEECCCCEEEEECCEEEEEEECHHHHHHHHHHCCHHHHHHHH
RNMQITGKREINEFLLEKGSTELKKPISLYELIKRPELDYFKVESLDDKRPSLSDDEKEE
HCCCCCCHHHHHHHHHHCCCHHHHCCHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCC
INIIAKYEGYINKQLEQVEQFKKYEDRLIPKSINYLDIKGLRLEAIQKLEKIKPINIGQA
EEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCC
SRISGVSPADISVLLIYMERKNREN
CCCCCCCCCCEEEEEEEEECCCCCC
>Mature Secondary Structure
MKYSAGDFDVVVIGAGHAGCEAALASARMGCKTLICTMNLDSIALMACNPNIGGTAKGHL
CCCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCCCCCCHH
VREIDALGGEMGINIDHTFIQSRMLNTSKGPAVHSLRAQADKKRYSERMKHLLEKEDNVV
HHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEE
LRQLEVIEIDVEDNEVKGVLTKNGAYFTTKAIILCTGTYLKGKIIIGDIIYSSGPSGLYP
EEEEEEEEEEECCCCEEEEEECCCCEEEEEEEEEEECCEEECEEEEEEEEECCCCCCCCC
ANDLSQSLLDLGINLRRFKTGTPARINKRSVDFSKMIEQPGDEKIVPFSFIHNKLDKDQI
CHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHCCCCCE
SCYLTYTSEETHKIIHENIHRSPLYNGSIEGVGPRYCPSIEDKIVRFPDKDKHQIFIEPE
EEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCCCCEEEEECC
GENTEELYVGGMSSSLPEDVQIKMYRSVPGLENAEILRTAYAIEYDCIDPQQLDLTLEFK
CCCCCEEEEECCCCCCCCCEEEEEEECCCCCCCHHHHHHEEEEEEECCCCCEEEEEEEEE
NINGLYGAGQFNGSSGYEEAAAQGLIAGINAVLKIKEKNPLILKRSDAYIGVLIDDLVTK
CCCCEEECCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCEEEEEHHHHHHC
GTNEPYRMMTSRAEYRLLLRQDNADLRLTELGYKVGLVKEDRYNKFLNRKKNVENEIERL
CCCCHHHHHHCCCCEEEEEEECCCCEEEEECCEEEEEEECHHHHHHHHHHCCHHHHHHHH
RNMQITGKREINEFLLEKGSTELKKPISLYELIKRPELDYFKVESLDDKRPSLSDDEKEE
HCCCCCCHHHHHHHHHHCCCHHHHCCHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCC
INIIAKYEGYINKQLEQVEQFKKYEDRLIPKSINYLDIKGLRLEAIQKLEKIKPINIGQA
EEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCC
SRISGVSPADISVLLIYMERKNREN
CCCCCCCCCCEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA