Click here to switch to the map view.

The map label for this gene is gidB

Identifier: 148381578

GI number: 148381578

Start: 3880246

End: 3880965

Strand: Reverse

Name: gidB

Synonym: CBO3641

Alternate gene names: 148381578

Gene position: 3880965-3880246 (Counterclockwise)

Preceding gene: 148381579

Following gene: 148381577

Centisome position: 99.85

GC content: 26.94

Gene sequence:

>720_bases
ATGGAATTTTTCAACATACTACAATCAGCATGCAATGATGTTAATTTAGATTTTAATGATAAAAAATATAATCAGCTTAT
AAGTTATAAAAATTTAATACAGGAATGGAATAAGAAAATAAATCTAACAGCTATTGTTGAAGATGATGAAATAATAAAAA
AACATTTTATAGATTGTATAAAAATATTTAAATCTTCACCTATAGGAGAAGCTAAAAGTTTAATAGATATAGGTACAGGT
GCAGGATTTCCAGGCATCCCCATAAAAATATTAAAAGAAGACATAGAAATAACTTTATTGGATTCCCTACAAAAGAGAAT
AAATTTTTTAAATACTGTAATAGGAGAGCTACAATTAAAAAATATACAATGTTTACATGGAAGAGCAGAAGATTATGCAC
AGGAAATTCAGCATAGGCAAAAATATGATATAGCAGTTTCTCGAGCAGTAGCGAATTTAGCAGTTTTAAGTGAATTTTGT
ATTCCTTTTGTAGAAAAAGGTGGATATTTTATAGCCATGAAAGGTCCGTCTGTAGAAGAAGAAATAACAGCAGCTACAAA
ATCTATAGAAATTTTAGGTGGAAAAATAGAGGATATAATAAAAATAGATATTGAAGATACAGATCTAAAACATAATCTAG
TAATCATAAAAAAAGTAAAAGAAACGGGAAAAAGGTATCCTAGGAAGCCAGGTATTATTAAAAAAAATCCTCTAAAATAA

Upstream 100 bases:

>100_bases
CTAGAATTTCAGGTGTATCTCCAGCAGATATATCAGTATTACTTATATATATGGAAAGAAAAAATAGAGAAAACTAGAAA
ATCCTTTTGGAGGTTTTTAA

Downstream 100 bases:

>100_bases
ATGTAATAATTTGCAATTAAAAAAATAATAAAAAAAGAAGGAAAATTACATTTAAAGAAGAAATGTAAATATTAGGAAGA
GAATAAGAGGGATGGTAATA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MEFFNILQSACNDVNLDFNDKKYNQLISYKNLIQEWNKKINLTAIVEDDEIIKKHFIDCIKIFKSSPIGEAKSLIDIGTG
AGFPGIPIKILKEDIEITLLDSLQKRINFLNTVIGELQLKNIQCLHGRAEDYAQEIQHRQKYDIAVSRAVANLAVLSEFC
IPFVEKGGYFIAMKGPSVEEEITAATKSIEILGGKIEDIIKIDIEDTDLKHNLVIIKKVKETGKRYPRKPGIIKKNPLK

Sequences:

>Translated_239_residues
MEFFNILQSACNDVNLDFNDKKYNQLISYKNLIQEWNKKINLTAIVEDDEIIKKHFIDCIKIFKSSPIGEAKSLIDIGTG
AGFPGIPIKILKEDIEITLLDSLQKRINFLNTVIGELQLKNIQCLHGRAEDYAQEIQHRQKYDIAVSRAVANLAVLSEFC
IPFVEKGGYFIAMKGPSVEEEITAATKSIEILGGKIEDIIKIDIEDTDLKHNLVIIKKVKETGKRYPRKPGIIKKNPLK
>Mature_239_residues
MEFFNILQSACNDVNLDFNDKKYNQLISYKNLIQEWNKKINLTAIVEDDEIIKKHFIDCIKIFKSSPIGEAKSLIDIGTG
AGFPGIPIKILKEDIEITLLDSLQKRINFLNTVIGELQLKNIQCLHGRAEDYAQEIQHRQKYDIAVSRAVANLAVLSEFC
IPFVEKGGYFIAMKGPSVEEEITAATKSIEILGGKIEDIIKIDIEDTDLKHNLVIIKKVKETGKRYPRKPGIIKKNPLK

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=218, Percent_Identity=33.9449541284404, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_CLOB1 (A7FPL8)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385955.1
- ProteinModelPortal:   A7FPL8
- SMR:   A7FPL8
- STRING:   A7FPL8
- GeneID:   5397284
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3734
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   HIMDSIV
- ProtClustDB:   PRK00107
- BioCyc:   CBOT441770:CLB_3734-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 27084; Mature: 27084

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFFNILQSACNDVNLDFNDKKYNQLISYKNLIQEWNKKINLTAIVEDDEIIKKHFIDCI
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHH
KIFKSSPIGEAKSLIDIGTGAGFPGIPIKILKEDIEITLLDSLQKRINFLNTVIGELQLK
HHHHCCCCCHHHHHHEECCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHC
NIQCLHGRAEDYAQEIQHRQKYDIAVSRAVANLAVLSEFCIPFVEKGGYFIAMKGPSVEE
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHH
EITAATKSIEILGGKIEDIIKIDIEDTDLKHNLVIIKKVKETGKRYPRKPGIIKKNPLK
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MEFFNILQSACNDVNLDFNDKKYNQLISYKNLIQEWNKKINLTAIVEDDEIIKKHFIDCI
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHH
KIFKSSPIGEAKSLIDIGTGAGFPGIPIKILKEDIEITLLDSLQKRINFLNTVIGELQLK
HHHHCCCCCHHHHHHEECCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHC
NIQCLHGRAEDYAQEIQHRQKYDIAVSRAVANLAVLSEFCIPFVEKGGYFIAMKGPSVEE
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHH
EITAATKSIEILGGKIEDIIKIDIEDTDLKHNLVIIKKVKETGKRYPRKPGIIKKNPLK
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA