BacMap

Definition	Clostridium botulinum A str. ATCC 3502, complete genome.
Accession	NC_009495
Length	3,886,916

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The map label for this gene is accC [H]

Identifier: 148381534

GI number: 148381534

Start: 3839770

End: 3841116

Strand: Reverse

Name: accC [H]

Synonym: CBO3596

Alternate gene names: 148381534

Gene position: 3841116-3839770 (Counterclockwise)

Preceding gene: 148381535

Following gene: 148381533

Centisome position: 98.82

GC content: 32.0

Gene sequence:

>1347_bases
GTGTTTAAGAAAATACTTGTGGCTAATAGAGGTGAGATTGCGGTTAGAATAATTAGAGCTTGTAGAGAAATGGGAATAGA
AACTGTAGCTATATATTCAGAGGCAGATAAAGATGCTCTACATGTTCAATTGGCAGATGAGGCAGTTTGTATAGGGCCTC
CTTCTTCAAAGGATAGTTATTTAAATATGTATAATATAATAAGTGCTACTGTATTAACAGGATCACAGGCTATTCATCCT
GGTTTTGGGTTCCTATCTGAGAATAGTAAGTTTGCTAATATGTGTAAAGATTGTAATATAGTTTTTATAGGACCAGACAG
CGAAACAATAGATAAAGTGGGAAATAAGTCCAATGCTAGAGATATAATGATAAGGGCAGGAGTGCCAGTTATTCCGGGTT
CTAATGGAGTAATACATAATGAAGAAGAAGCTTTAAATATAGCAGAAGAAATAGGTTACCCAGTAGTTGTTAAAGCTTCA
GCAGGCGGTGGAGGACGTGGAATAAGAATAGTTCATTCCAAGGAAAATATGATAAAAGCTTTTAACACTGCAAAATCTGA
AGCTAAAGGTGCTTTTGGTGATGATAGTATGTATGTAGAAAAATTCATAAAAAATCCAAGGCATATAGAATTTCAAATAC
TAGGAGATAACTATGGTAATATAATACACCTAGGAGAAAGAGATTGTTCTCTTCAAAGAAGGAATCAAAAGGTGTTAGAA
GAAGCTCCATCTCCTCGTATGAATGAAGAATTAAGAAAAAGAATGGGGGATGTTGCTATAAAGGCAGCAAAGGCTGTTGA
GTATAAAAATGCTGGTACTATAGAATTCTTACTAGATGAAGATGAAAGTTTTTATTTTATGGAAATGAATACAAGAATAC
AAGTAGAACATCCAATAACAGAAATGGTAACAGGTGTAGATATTTTAAAAGAACAAATAAAAATAGCTTATGGAGAAAAA
TTAAATATAAAACAAAAAGATATAAAAATACAAGGGCATGCTATTGAATGCAGAATAAATGCAGAAGACTATAAAAATGG
ATTTAGACCTTGTCCTGGTAAAATAGAGAATTTATACATCCCAGGAGGTTTGGGAGTAAGATTAGATAGTTCAGTATATT
CAGGATATACTATACCTCCATACTATGACTCTATGATAGGTAAACTTATAGCTTATGGGAGGAATAGAGAAGAAACTATA
CAAATTATGAAAAGAGCATTAGGAGAATTGATTATAGAAGGTGTAAACACCAATATAGATTTTCAATTTATTATATTAGA
GGATGAAAATTTTATAAAGGGAGAATACACTACAAAATATATAGAAAAAATGCTAACTGACAATTAG

Upstream 100 bases:

>100_bases
AAGGCCGTAGCTACTGTAGATGGGAAAGTAGCTGCAGAAGCAGAATTATTATTTGTAATAGGAAAATAAATCTATAAAAA
TAGAAGGGGAGAAGATAGCA

Downstream 100 bases:

>100_bases
TGTAGTCAGGGGGAAGTATATGTTAAAAAATTTATTTAGAAAAACAAAGTATATAACTGTAAGTCAAAAGAATATAGAAA
ATTATAAAAGAGAAAATACT

Product: biotin carboxylase

Products: NA

Alternate protein names: Acetyl-CoA carboxylase subunit A; ACC [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MFKKILVANRGEIAVRIIRACREMGIETVAIYSEADKDALHVQLADEAVCIGPPSSKDSYLNMYNIISATVLTGSQAIHP
GFGFLSENSKFANMCKDCNIVFIGPDSETIDKVGNKSNARDIMIRAGVPVIPGSNGVIHNEEEALNIAEEIGYPVVVKAS
AGGGGRGIRIVHSKENMIKAFNTAKSEAKGAFGDDSMYVEKFIKNPRHIEFQILGDNYGNIIHLGERDCSLQRRNQKVLE
EAPSPRMNEELRKRMGDVAIKAAKAVEYKNAGTIEFLLDEDESFYFMEMNTRIQVEHPITEMVTGVDILKEQIKIAYGEK
LNIKQKDIKIQGHAIECRINAEDYKNGFRPCPGKIENLYIPGGLGVRLDSSVYSGYTIPPYYDSMIGKLIAYGRNREETI
QIMKRALGELIIEGVNTNIDFQFIILEDENFIKGEYTTKYIEKMLTDN

Sequences:

>Translated_448_residues
MFKKILVANRGEIAVRIIRACREMGIETVAIYSEADKDALHVQLADEAVCIGPPSSKDSYLNMYNIISATVLTGSQAIHP
GFGFLSENSKFANMCKDCNIVFIGPDSETIDKVGNKSNARDIMIRAGVPVIPGSNGVIHNEEEALNIAEEIGYPVVVKAS
AGGGGRGIRIVHSKENMIKAFNTAKSEAKGAFGDDSMYVEKFIKNPRHIEFQILGDNYGNIIHLGERDCSLQRRNQKVLE
EAPSPRMNEELRKRMGDVAIKAAKAVEYKNAGTIEFLLDEDESFYFMEMNTRIQVEHPITEMVTGVDILKEQIKIAYGEK
LNIKQKDIKIQGHAIECRINAEDYKNGFRPCPGKIENLYIPGGLGVRLDSSVYSGYTIPPYYDSMIGKLIAYGRNREETI
QIMKRALGELIIEGVNTNIDFQFIILEDENFIKGEYTTKYIEKMLTDN
>Mature_448_residues
MFKKILVANRGEIAVRIIRACREMGIETVAIYSEADKDALHVQLADEAVCIGPPSSKDSYLNMYNIISATVLTGSQAIHP
GFGFLSENSKFANMCKDCNIVFIGPDSETIDKVGNKSNARDIMIRAGVPVIPGSNGVIHNEEEALNIAEEIGYPVVVKAS
AGGGGRGIRIVHSKENMIKAFNTAKSEAKGAFGDDSMYVEKFIKNPRHIEFQILGDNYGNIIHLGERDCSLQRRNQKVLE
EAPSPRMNEELRKRMGDVAIKAAKAVEYKNAGTIEFLLDEDESFYFMEMNTRIQVEHPITEMVTGVDILKEQIKIAYGEK
LNIKQKDIKIQGHAIECRINAEDYKNGFRPCPGKIENLYIPGGLGVRLDSSVYSGYTIPPYYDSMIGKLIAYGRNREETI
QIMKRALGELIIEGVNTNIDFQFIILEDENFIKGEYTTKYIEKMLTDN

Specific function: This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA [H]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI65506442, Length=451, Percent_Identity=50.3325942350333, Blast_Score=453, Evalue=1e-127,
Organism=Homo sapiens, GI189095269, Length=451, Percent_Identity=50.3325942350333, Blast_Score=453, Evalue=1e-127,
Organism=Homo sapiens, GI295821183, Length=451, Percent_Identity=50.3325942350333, Blast_Score=453, Evalue=1e-127,
Organism=Homo sapiens, GI116805327, Length=441, Percent_Identity=46.4852607709751, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI106049528, Length=446, Percent_Identity=47.3094170403587, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI106049295, Length=446, Percent_Identity=47.3094170403587, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI106049292, Length=446, Percent_Identity=47.3094170403587, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI38679974, Length=508, Percent_Identity=32.2834645669291, Blast_Score=262, Evalue=5e-70,
Organism=Homo sapiens, GI38679977, Length=508, Percent_Identity=32.2834645669291, Blast_Score=262, Evalue=5e-70,
Organism=Homo sapiens, GI38679967, Length=508, Percent_Identity=32.2834645669291, Blast_Score=262, Evalue=5e-70,
Organism=Homo sapiens, GI38679960, Length=508, Percent_Identity=32.2834645669291, Blast_Score=262, Evalue=5e-70,
Organism=Homo sapiens, GI38679971, Length=508, Percent_Identity=32.2834645669291, Blast_Score=262, Evalue=5e-70,
Organism=Homo sapiens, GI134142062, Length=509, Percent_Identity=31.434184675835, Blast_Score=251, Evalue=9e-67,
Organism=Escherichia coli, GI1789654, Length=446, Percent_Identity=55.6053811659193, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI1786216, Length=244, Percent_Identity=22.9508196721311, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17567343, Length=442, Percent_Identity=47.9638009049774, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71987519, Length=440, Percent_Identity=46.3636363636364, Blast_Score=418, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17562816, Length=449, Percent_Identity=46.5478841870824, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI71997168, Length=508, Percent_Identity=29.3307086614173, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI71997163, Length=508, Percent_Identity=29.3307086614173, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI133931226, Length=486, Percent_Identity=31.2757201646091, Blast_Score=217, Evalue=9e-57,
Organism=Saccharomyces cerevisiae, GI6319685, Length=439, Percent_Identity=43.5079726651481, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6319695, Length=446, Percent_Identity=47.085201793722, Blast_Score=378, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321376, Length=446, Percent_Identity=47.7578475336323, Blast_Score=367, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324343, Length=514, Percent_Identity=30.9338521400778, Blast_Score=242, Evalue=8e-65,
Organism=Saccharomyces cerevisiae, GI6323863, Length=491, Percent_Identity=31.9755600814664, Blast_Score=236, Evalue=7e-63,
Organism=Drosophila melanogaster, GI24651757, Length=444, Percent_Identity=45.2702702702703, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI281363050, Length=448, Percent_Identity=47.3214285714286, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24652224, Length=448, Percent_Identity=47.3214285714286, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24652222, Length=448, Percent_Identity=47.3214285714286, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24652220, Length=448, Percent_Identity=47.3214285714286, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24652218, Length=448, Percent_Identity=47.3214285714286, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24652212, Length=448, Percent_Identity=47.3214285714286, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24652210, Length=448, Percent_Identity=47.3214285714286, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24652214, Length=448, Percent_Identity=47.3214285714286, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI19921944, Length=448, Percent_Identity=47.3214285714286, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24652216, Length=448, Percent_Identity=47.3214285714286, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24651759, Length=408, Percent_Identity=44.6078431372549, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI161076409, Length=500, Percent_Identity=30.8, Blast_Score=249, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24586458, Length=500, Percent_Identity=30.8, Blast_Score=248, Evalue=5e-66,
Organism=Drosophila melanogaster, GI161076407, Length=500, Percent_Identity=30.8, Blast_Score=248, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24586460, Length=500, Percent_Identity=30.8, Blast_Score=248, Evalue=5e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.3.4.14; =6.4.1.2 [H]

Molecular weight: Translated: 50043; Mature: 50043

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2 ; PS50979 BC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKILVANRGEIAVRIIRACREMGIETVAIYSEADKDALHVQLADEAVCIGPPSSKDSY
CCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEEECCEEEECCCCCCCCH
LNMYNIISATVLTGSQAIHPGFGFLSENSKFANMCKDCNIVFIGPDSETIDKVGNKSNAR
HHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECCCCHHHHHHCCCCCCC
DIMIRAGVPVIPGSNGVIHNEEEALNIAEEIGYPVVVKASAGGGGRGIRIVHSKENMIKA
EEEEECCCEECCCCCCCEECHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCHHHHH
FNTAKSEAKGAFGDDSMYVEKFIKNPRHIEFQILGDNYGNIIHLGERDCSLQRRNQKVLE
HHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHH
EAPSPRMNEELRKRMGDVAIKAAKAVEYKNAGTIEFLLDEDESFYFMEMNTRIQVEHPIT
HCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCCEEEEEECCEEEEECCHH
EMVTGVDILKEQIKIAYGEKLNIKQKDIKIQGHAIECRINAEDYKNGFRPCPGKIENLYI
HHHHHHHHHHHHHHHHCCCCCCCEECCEEEECEEEEEEECHHHHCCCCCCCCCCCCEEEE
PGGLGVRLDSSVYSGYTIPPYYDSMIGKLIAYGRNREETIQIMKRALGELIIEGVNTNID
CCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
FQFIILEDENFIKGEYTTKYIEKMLTDN
EEEEEECCCCEECCCHHHHHHHHHHCCC
>Mature Secondary Structure
MFKKILVANRGEIAVRIIRACREMGIETVAIYSEADKDALHVQLADEAVCIGPPSSKDSY
CCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEEECCEEEECCCCCCCCH
LNMYNIISATVLTGSQAIHPGFGFLSENSKFANMCKDCNIVFIGPDSETIDKVGNKSNAR
HHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECCCCHHHHHHCCCCCCC
DIMIRAGVPVIPGSNGVIHNEEEALNIAEEIGYPVVVKASAGGGGRGIRIVHSKENMIKA
EEEEECCCEECCCCCCCEECHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCHHHHH
FNTAKSEAKGAFGDDSMYVEKFIKNPRHIEFQILGDNYGNIIHLGERDCSLQRRNQKVLE
HHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHH
EAPSPRMNEELRKRMGDVAIKAAKAVEYKNAGTIEFLLDEDESFYFMEMNTRIQVEHPIT
HCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCCEEEEEECCEEEEECCHH
EMVTGVDILKEQIKIAYGEKLNIKQKDIKIQGHAIECRINAEDYKNGFRPCPGKIENLYI
HHHHHHHHHHHHHHHHCCCCCCCEECCEEEECEEEEEEECHHHHCCCCCCCCCCCCEEEE
PGGLGVRLDSSVYSGYTIPPYYDSMIGKLIAYGRNREETIQIMKRALGELIIEGVNTNID
CCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
FQFIILEDENFIKGEYTTKYIEKMLTDN
EEEEEECCCCEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8102363; 11759840 [H]