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The map label for this gene is cwlD [H]

Identifier: 148381383

GI number: 148381383

Start: 3684828

End: 3685532

Strand: Reverse

Name: cwlD [H]

Synonym: CBO3443

Alternate gene names: 148381383

Gene position: 3685532-3684828 (Counterclockwise)

Preceding gene: 148381384

Following gene: 148381382

Centisome position: 94.82

GC content: 27.09

Gene sequence:

>705_bases
TTGAAGATTTATAATAAGAAATTAGTATTAACAACCATTATAATATTTATAAGTCTTGGTTTTATAATAAGGATTAATAT
TTTCAGCAATTCAAATAGTGTTAAAGGAAAAAAGATTATTCTTATAGATCCAGGTCATGGTGGAATGGATGGAGGAGCAG
TAGCTAGAGATGGTACTTTGGAGAAGGATGTAAACTTAGAGATAAGTAAAATATTAAAGAAAAATCTTAAAAAAGAAGGT
TATAAGGTTGTAATGACGAGAGAAGATGACAGTGGTCTATATTCAGAAGATAAAGGGAAAAGAGTTAGACAGAAGAAAAT
AGAAGACTTGAATAAAAGATGTGAACTTAAAAGAACTACTAAATGTGATATGTTTATAAGCATACATTTAAATATGTTTG
ATCAAAGCCAATATTATGGCGCTCAAGTATGGTATTCAAATAATGAAGATAGTAAAAGGCTTGCACATATATTACAAAAT
AATTTAAGGGAAGAATTAGACGAAAACAATAAAAGAAAAGAAAAAGCAGCAAAAAATAGTTATAAAATATTAAGGAATAA
TGATGAAATGCCTTCAGTTATTGTAGAATGTGGTTTTTTATCTAATTCCATGGAACTAGAAAAATTGAAAAGTACAGATT
ATCAAGAGAAGATATCAAAAGCTATAGTTAAATCAGTAGGTGATTATTTCAATGATAAAGAGTAA

Upstream 100 bases:

>100_bases
AATTTTTTATAAGCTTAATGTGAAACTATATTAATCATATTATAAATTTTAATGATATAGCATAGTATTAAATGAAGTAC
TAATATATAAGGAGGAAACT

Downstream 100 bases:

>100_bases
AAATAAGTTCATATATTTCCAAGGAAATATATGAACTTATTTTTATAAAGACCGATGTAGATTGATGTAGAAAAAAACAT
GTAATTAAACTATAATATTC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKIYNKKLVLTTIIIFISLGFIIRINIFSNSNSVKGKKIILIDPGHGGMDGGAVARDGTLEKDVNLEISKILKKNLKKEG
YKVVMTREDDSGLYSEDKGKRVRQKKIEDLNKRCELKRTTKCDMFISIHLNMFDQSQYYGAQVWYSNNEDSKRLAHILQN
NLREELDENNKRKEKAAKNSYKILRNNDEMPSVIVECGFLSNSMELEKLKSTDYQEKISKAIVKSVGDYFNDKE

Sequences:

>Translated_234_residues
MKIYNKKLVLTTIIIFISLGFIIRINIFSNSNSVKGKKIILIDPGHGGMDGGAVARDGTLEKDVNLEISKILKKNLKKEG
YKVVMTREDDSGLYSEDKGKRVRQKKIEDLNKRCELKRTTKCDMFISIHLNMFDQSQYYGAQVWYSNNEDSKRLAHILQN
NLREELDENNKRKEKAAKNSYKILRNNDEMPSVIVECGFLSNSMELEKLKSTDYQEKISKAIVKSVGDYFNDKE
>Mature_234_residues
MKIYNKKLVLTTIIIFISLGFIIRINIFSNSNSVKGKKIILIDPGHGGMDGGAVARDGTLEKDVNLEISKILKKNLKKEG
YKVVMTREDDSGLYSEDKGKRVRQKKIEDLNKRCELKRTTKCDMFISIHLNMFDQSQYYGAQVWYSNNEDSKRLAHILQN
NLREELDENNKRKEKAAKNSYKILRNNDEMPSVIVECGFLSNSMELEKLKSTDYQEKISKAIVKSVGDYFNDKE

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=238, Percent_Identity=27.3109243697479, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87082163, Length=237, Percent_Identity=27.4261603375527, Blast_Score=87, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR014234 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 26972; Mature: 26972

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYNKKLVLTTIIIFISLGFIIRINIFSNSNSVKGKKIILIDPGHGGMDGGAVARDGTL
CCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
EKDVNLEISKILKKNLKKEGYKVVMTREDDSGLYSEDKGKRVRQKKIEDLNKRCELKRTT
CCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
KCDMFISIHLNMFDQSQYYGAQVWYSNNEDSKRLAHILQNNLREELDENNKRKEKAAKNS
CCEEEEEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
YKILRNNDEMPSVIVECGFLSNSMELEKLKSTDYQEKISKAIVKSVGDYFNDKE
HHEECCCCCCHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKIYNKKLVLTTIIIFISLGFIIRINIFSNSNSVKGKKIILIDPGHGGMDGGAVARDGTL
CCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
EKDVNLEISKILKKNLKKEGYKVVMTREDDSGLYSEDKGKRVRQKKIEDLNKRCELKRTT
CCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
KCDMFISIHLNMFDQSQYYGAQVWYSNNEDSKRLAHILQNNLREELDENNKRKEKAAKNS
CCEEEEEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
YKILRNNDEMPSVIVECGFLSNSMELEKLKSTDYQEKISKAIVKSVGDYFNDKE
HHEECCCCCCHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7559346; 8969501; 9384377 [H]