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The map label for this gene is celF [H]

Identifier: 148381301

GI number: 148381301

Start: 3593186

End: 3594523

Strand: Reverse

Name: celF [H]

Synonym: CBO3360

Alternate gene names: 148381301

Gene position: 3594523-3593186 (Counterclockwise)

Preceding gene: 148381302

Following gene: 148381300

Centisome position: 92.48

GC content: 31.24

Gene sequence:

>1338_bases
ATGGATAATGGCATAAAAATAGTAACTATAGGTGGGGGATCAAGTTATACACCAGAATTAGTTGAAGGATTTATAAAGAG
GTATAATGAGTTACCAGTTAGGGAATTGTGGTTAGTAGATATAGAGGAAGGAAGAGAAAAGTTACAGATTGTAGGGAATT
TGGCTAAAAGGATGGTAAGAAAAGCAGGAATACCAATGGAAATACATTTAACATTAGATAGGAAAAAAGCATTAAAAGAT
GCTGATTTTGTAACAACACAAATAAGAGTAGGATTTTTAGATGCAAGAATAAAAGATGAAAAAATTCCTTTAAATCATGG
TGTTATAGGGCAAGAAACTAATGGGGCAGGGGGAATGTTTAAAGCATTAAGGACAATCCCTGTAATTCTAGATATAGATA
AGGATATGAGTAAATTGTGCCCTAATGCTTGGATGATAAATTTTACTAATCCAGCAGGTATGGTTACAGAAGCATTATTA
AGATATGGAAAAAATAAAAAAGTTATAGGTCTTTGTAATGTTCCTATAGGTATGGAGAAGGCAGCTGCAAAAGTTTTAGA
GGTTGAACATGAAAGACTAAGGATGGATTTTGTAGGATTGAACCATATGGTGTTCGGAAAAGAAGTATATGTAGATGGGG
TAAAGGTTACAGATAAATTAATTAACTTATTAGCTGATGGAAGTATAGAACAAATAGTTAAAAATGTACCGGATTTTACA
TGGAATAAAGATTTTATAAAAGCTTTAGGAATGATGCCATGTCCATATCACAGATATTATTTTCAAACAAAAGATATGCT
AAAAGAAGAACTAGAACAATTTTCTAAAGATGAAACTAGAGCAGAGGTTGTTAAAAAAGTAGAAGAAGAGTTGTTTAAAC
TTTATAAAGATGAAAATTTGGATATTAAACCTCCTGAGTTAGAGAAAAGAGGAGGAGCCTATTATAGTGATGCAGCTTGT
AGGCTTATATGTTCAATATATAATGATAAAAAAGATATTCAACCAGTAAATGTAAGAAATAATGGAGCTATAATAGGCAT
TCCAAATGAATCTGCTATAGAAATATCATGTATGATAACAAGAGATGGACCTAAGGCAATAGCAGTAGGGGAATTGCCAA
TAGCAATAAATGGACTTATACAACAGATAAAAACCTTTGAGAGATTAGTAGTAGATTCAGCAGTTTTTGGTGATTATTAT
AAGGCGATATTAGCATTGACTGTAAATCCTTTAGTTCAATCAGATAAGCTTGCAAAGACATTGGTAGATGAACTTATGAT
AGCTCATAAGGATTATTTACCACAGTTTAAAGAACTTATAGAAGAAAATTTTAAATAA

Upstream 100 bases:

>100_bases
TTATACAATACATGAACAAGTGGATTTGGTAATAAAAAGGTATTGGCATGATGTTTGCTATATATAATAGTGTACATATA
AAACACTGGGGGTGTGTTCT

Downstream 100 bases:

>100_bases
CGATCATTTCAAAATAGATTACTATTATTAATTATTTATAAATATATAAAAAATATGTATAGAAAAAGGGGAGGGTTAGA
AATGAAAAAGTTCTTTGACT

Product: glycosyl hydrolase, family 4

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MDNGIKIVTIGGGSSYTPELVEGFIKRYNELPVRELWLVDIEEGREKLQIVGNLAKRMVRKAGIPMEIHLTLDRKKALKD
ADFVTTQIRVGFLDARIKDEKIPLNHGVIGQETNGAGGMFKALRTIPVILDIDKDMSKLCPNAWMINFTNPAGMVTEALL
RYGKNKKVIGLCNVPIGMEKAAAKVLEVEHERLRMDFVGLNHMVFGKEVYVDGVKVTDKLINLLADGSIEQIVKNVPDFT
WNKDFIKALGMMPCPYHRYYFQTKDMLKEELEQFSKDETRAEVVKKVEEELFKLYKDENLDIKPPELEKRGGAYYSDAAC
RLICSIYNDKKDIQPVNVRNNGAIIGIPNESAIEISCMITRDGPKAIAVGELPIAINGLIQQIKTFERLVVDSAVFGDYY
KAILALTVNPLVQSDKLAKTLVDELMIAHKDYLPQFKELIEENFK

Sequences:

>Translated_445_residues
MDNGIKIVTIGGGSSYTPELVEGFIKRYNELPVRELWLVDIEEGREKLQIVGNLAKRMVRKAGIPMEIHLTLDRKKALKD
ADFVTTQIRVGFLDARIKDEKIPLNHGVIGQETNGAGGMFKALRTIPVILDIDKDMSKLCPNAWMINFTNPAGMVTEALL
RYGKNKKVIGLCNVPIGMEKAAAKVLEVEHERLRMDFVGLNHMVFGKEVYVDGVKVTDKLINLLADGSIEQIVKNVPDFT
WNKDFIKALGMMPCPYHRYYFQTKDMLKEELEQFSKDETRAEVVKKVEEELFKLYKDENLDIKPPELEKRGGAYYSDAAC
RLICSIYNDKKDIQPVNVRNNGAIIGIPNESAIEISCMITRDGPKAIAVGELPIAINGLIQQIKTFERLVVDSAVFGDYY
KAILALTVNPLVQSDKLAKTLVDELMIAHKDYLPQFKELIEENFK
>Mature_445_residues
MDNGIKIVTIGGGSSYTPELVEGFIKRYNELPVRELWLVDIEEGREKLQIVGNLAKRMVRKAGIPMEIHLTLDRKKALKD
ADFVTTQIRVGFLDARIKDEKIPLNHGVIGQETNGAGGMFKALRTIPVILDIDKDMSKLCPNAWMINFTNPAGMVTEALL
RYGKNKKVIGLCNVPIGMEKAAAKVLEVEHERLRMDFVGLNHMVFGKEVYVDGVKVTDKLINLLADGSIEQIVKNVPDFT
WNKDFIKALGMMPCPYHRYYFQTKDMLKEELEQFSKDETRAEVVKKVEEELFKLYKDENLDIKPPELEKRGGAYYSDAAC
RLICSIYNDKKDIQPVNVRNNGAIIGIPNESAIEISCMITRDGPKAIAVGELPIAINGLIQQIKTFERLVVDSAVFGDYY
KAILALTVNPLVQSDKLAKTLVDELMIAHKDYLPQFKELIEENFK

Specific function: Hydrolyzes phospho-beta-glucosides (Probable) [H]

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=445, Percent_Identity=51.9101123595506, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI1790560, Length=465, Percent_Identity=27.0967741935484, Blast_Score=140, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 50225; Mature: 50225

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNGIKIVTIGGGSSYTPELVEGFIKRYNELPVRELWLVDIEEGREKLQIVGNLAKRMVR
CCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHH
KAGIPMEIHLTLDRKKALKDADFVTTQIRVGFLDARIKDEKIPLNHGVIGQETNGAGGMF
HCCCCEEEEEEECHHHHHCCCHHEEEEEEEEEEECEECCCCCCCCCCCCCCCCCCCCHHH
KALRTIPVILDIDKDMSKLCPNAWMINFTNPAGMVTEALLRYGKNKKVIGLCNVPIGMEK
HHHHHCCEEEECCCCHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCHHH
AAAKVLEVEHERLRMDFVGLNHMVFGKEVYVDGVKVTDKLINLLADGSIEQIVKNVPDFT
HHHHHHHHHHHHHHHHHCCCCEEEECCEEEECCHHHHHHHHHHHCCCCHHHHHHHCCCCC
WNKDFIKALGMMPCPYHRYYFQTKDMLKEELEQFSKDETRAEVVKKVEEELFKLYKDENL
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
DIKPPELEKRGGAYYSDAACRLICSIYNDKKDIQPVNVRNNGAIIGIPNESAIEISCMIT
CCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCEEEECCCCCEEEEEEEEE
RDGPKAIAVGELPIAINGLIQQIKTFERLVVDSAVFGDYYKAILALTVNPLVQSDKLAKT
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVDELMIAHKDYLPQFKELIEENFK
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDNGIKIVTIGGGSSYTPELVEGFIKRYNELPVRELWLVDIEEGREKLQIVGNLAKRMVR
CCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHH
KAGIPMEIHLTLDRKKALKDADFVTTQIRVGFLDARIKDEKIPLNHGVIGQETNGAGGMF
HCCCCEEEEEEECHHHHHCCCHHEEEEEEEEEEECEECCCCCCCCCCCCCCCCCCCCHHH
KALRTIPVILDIDKDMSKLCPNAWMINFTNPAGMVTEALLRYGKNKKVIGLCNVPIGMEK
HHHHHCCEEEECCCCHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCHHH
AAAKVLEVEHERLRMDFVGLNHMVFGKEVYVDGVKVTDKLINLLADGSIEQIVKNVPDFT
HHHHHHHHHHHHHHHHHCCCCEEEECCEEEECCHHHHHHHHHHHCCCCHHHHHHHCCCCC
WNKDFIKALGMMPCPYHRYYFQTKDMLKEELEQFSKDETRAEVVKKVEEELFKLYKDENL
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
DIKPPELEKRGGAYYSDAACRLICSIYNDKKDIQPVNVRNNGAIIGIPNESAIEISCMIT
CCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCEEEECCCCCEEEEEEEEE
RDGPKAIAVGELPIAINGLIQQIKTFERLVVDSAVFGDYYKAILALTVNPLVQSDKLAKT
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVDELMIAHKDYLPQFKELIEENFK
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990303; 9384377; 8969509 [H]