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The map label for this gene is celB [H]

Identifier: 148381300

GI number: 148381300

Start: 3591812

End: 3593104

Strand: Reverse

Name: celB [H]

Synonym: CBO3359

Alternate gene names: 148381300

Gene position: 3593104-3591812 (Counterclockwise)

Preceding gene: 148381301

Following gene: 148381299

Centisome position: 92.44

GC content: 32.95

Gene sequence:

>1293_bases
ATGAAAAAGTTCTTTGACTGGATGGAAGAGCATTTTGTTCCAATAGCAGCTAAAATAGGTTCTCAAAGGCATTTAGTTGC
TATTCGTGATGGTTTTGCTACAATTACTCCAATTATTATGGCAGGGGCTTTTGCAGTATTATTTAATAATTTAGGATGGA
AACCATATCAAAATTTTATGAATTGGTTATTACCTGCTAATTGGAAAAATTTTGGTGATGGAGTCTGGAATGGTACTTTC
GCTATAATGTCATTATTAGTAGTATTTACTATATCCTATCATTTAGCTAAATCCTATGAAAAAGATGGGTTATCAGCGGG
AATTGTATCTCTATCTGCATTATTAATTTTATATAAGCCAACAAAAGATGGAGGGGCAGTACCACTTGAGTTTTTAGGGG
GCCAAGGATTATTTGTTGCATTAATAGTTGCATTAATAGCTACAGAAATTTTTGTTAAATTAGCAGGGAATCCAAAATTA
ATTATTAAAATGCCAGAGGGAGTTCCACCAGCAGTAGCAAAATCTTTTGCAGCATTATTACCATCAATTATAGTTTTAGC
TATTACAGCAGCAGTAAAGCAAATTTTCGGGGCAATAGGAATACCAGATATACACAAAGCATTATTTTCAGCATTACAGG
CACCATTGCAAGGTGTAATGGGAAGTTTGGGTGGTTTATTAGTTTTAGTTTTAGTACAGCAATTCTTATGGTTTTTTGGC
TTACATGGTTCAAATATACTTGCACCAATTATAAATGCGGTATTGTTACCATTAACAGAAGCTAATGTAAGAGCATTTAA
GAATGGAGTTAATCCTGAGCATATAATAAATAGTCAATTTTTAGATTCTTACGTAAATATGGGGGGATCAGGTGCCACTA
TAGCTTTATTAATTGCAATATTCATAATTGGAAGAAAGAGCAAATCACAAAAGACTATTGCAAATCTAAGTATAGCACCA
GGAATGTTCAATATAAATGAACCTGTTATATTCGGTTTACCAATAATTTTAAATCCAATATACTTTATACCATTTATACT
AGCACCATTGGCATCTGGAGTGATTGCATATGTATTAACTTTTATAGGGTTTGCACCTAAAGTTGTAGTAATGGCTCACT
GGACGACACCACCAATATTAGGAGCCATTATATCAACAAATTCACTAAGGGGCGGAATTACAGCTCTTATTTGTATGGTA
GTCAGCATAATAATATATATACCATTTGTTCGCATAGCTACAAAGAAAGACCAAAAAAATACAGTAGAGGTAGAGAAAAC
ACATTCAATTTAA

Upstream 100 bases:

>100_bases
AGAAGAAAATTTTAAATAACGATCATTTCAAAATAGATTACTATTATTAATTATTTATAAATATATAAAAAATATGTATA
GAAAAAGGGGAGGGTTAGAA

Downstream 100 bases:

>100_bases
CAACATTTGGTTATAATAAAAGCCGTATGCAATGATGCATACGGTTTTAAATATTTTCCATTTTTGCATAAATTTAATAT
GTTACAATATAATTAGATAA

Product: PTS system, lactose/cellobiose family, IIC component

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIC-Lic; PTS system lichenan-specific EIIC component [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKKFFDWMEEHFVPIAAKIGSQRHLVAIRDGFATITPIIMAGAFAVLFNNLGWKPYQNFMNWLLPANWKNFGDGVWNGTF
AIMSLLVVFTISYHLAKSYEKDGLSAGIVSLSALLILYKPTKDGGAVPLEFLGGQGLFVALIVALIATEIFVKLAGNPKL
IIKMPEGVPPAVAKSFAALLPSIIVLAITAAVKQIFGAIGIPDIHKALFSALQAPLQGVMGSLGGLLVLVLVQQFLWFFG
LHGSNILAPIINAVLLPLTEANVRAFKNGVNPEHIINSQFLDSYVNMGGSGATIALLIAIFIIGRKSKSQKTIANLSIAP
GMFNINEPVIFGLPIILNPIYFIPFILAPLASGVIAYVLTFIGFAPKVVVMAHWTTPPILGAIISTNSLRGGITALICMV
VSIIIYIPFVRIATKKDQKNTVEVEKTHSI

Sequences:

>Translated_430_residues
MKKFFDWMEEHFVPIAAKIGSQRHLVAIRDGFATITPIIMAGAFAVLFNNLGWKPYQNFMNWLLPANWKNFGDGVWNGTF
AIMSLLVVFTISYHLAKSYEKDGLSAGIVSLSALLILYKPTKDGGAVPLEFLGGQGLFVALIVALIATEIFVKLAGNPKL
IIKMPEGVPPAVAKSFAALLPSIIVLAITAAVKQIFGAIGIPDIHKALFSALQAPLQGVMGSLGGLLVLVLVQQFLWFFG
LHGSNILAPIINAVLLPLTEANVRAFKNGVNPEHIINSQFLDSYVNMGGSGATIALLIAIFIIGRKSKSQKTIANLSIAP
GMFNINEPVIFGLPIILNPIYFIPFILAPLASGVIAYVLTFIGFAPKVVVMAHWTTPPILGAIISTNSLRGGITALICMV
VSIIIYIPFVRIATKKDQKNTVEVEKTHSI
>Mature_430_residues
MKKFFDWMEEHFVPIAAKIGSQRHLVAIRDGFATITPIIMAGAFAVLFNNLGWKPYQNFMNWLLPANWKNFGDGVWNGTF
AIMSLLVVFTISYHLAKSYEKDGLSAGIVSLSALLILYKPTKDGGAVPLEFLGGQGLFVALIVALIATEIFVKLAGNPKL
IIKMPEGVPPAVAKSFAALLPSIIVLAITAAVKQIFGAIGIPDIHKALFSALQAPLQGVMGSLGGLLVLVLVQQFLWFFG
LHGSNILAPIINAVLLPLTEANVRAFKNGVNPEHIINSQFLDSYVNMGGSGATIALLIAIFIIGRKSKSQKTIANLSIAP
GMFNINEPVIFGLPIILNPIYFIPFILAPLASGVIAYVLTFIGFAPKVVVMAHWTTPPILGAIISTNSLRGGITALICMV
VSIIIYIPFVRIATKKDQKNTVEVEKTHSI

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=444, Percent_Identity=33.3333333333333, Blast_Score=226, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: NA

Molecular weight: Translated: 46325; Mature: 46325

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFFDWMEEHFVPIAAKIGSQRHLVAIRDGFATITPIIMAGAFAVLFNNLGWKPYQNFM
CCHHHHHHHHHCCHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
NWLLPANWKNFGDGVWNGTFAIMSLLVVFTISYHLAKSYEKDGLSAGIVSLSALLILYKP
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEC
TKDGGAVPLEFLGGQGLFVALIVALIATEIFVKLAGNPKLIIKMPEGVPPAVAKSFAALL
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
PSIIVLAITAAVKQIFGAIGIPDIHKALFSALQAPLQGVMGSLGGLLVLVLVQQFLWFFG
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LHGSNILAPIINAVLLPLTEANVRAFKNGVNPEHIINSQFLDSYVNMGGSGATIALLIAI
CCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
FIIGRKSKSQKTIANLSIAPGMFNINEPVIFGLPIILNPIYFIPFILAPLASGVIAYVLT
HHHCCCCCCHHHHHHCEECCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIGFAPKVVVMAHWTTPPILGAIISTNSLRGGITALICMVVSIIIYIPFVRIATKKDQKN
HHCCCCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TVEVEKTHSI
CEEEHHCCCC
>Mature Secondary Structure
MKKFFDWMEEHFVPIAAKIGSQRHLVAIRDGFATITPIIMAGAFAVLFNNLGWKPYQNFM
CCHHHHHHHHHCCHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
NWLLPANWKNFGDGVWNGTFAIMSLLVVFTISYHLAKSYEKDGLSAGIVSLSALLILYKP
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEC
TKDGGAVPLEFLGGQGLFVALIVALIATEIFVKLAGNPKLIIKMPEGVPPAVAKSFAALL
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
PSIIVLAITAAVKQIFGAIGIPDIHKALFSALQAPLQGVMGSLGGLLVLVLVQQFLWFFG
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LHGSNILAPIINAVLLPLTEANVRAFKNGVNPEHIINSQFLDSYVNMGGSGATIALLIAI
CCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
FIIGRKSKSQKTIANLSIAPGMFNINEPVIFGLPIILNPIYFIPFILAPLASGVIAYVLT
HHHCCCCCCHHHHHHCEECCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIGFAPKVVVMAHWTTPPILGAIISTNSLRGGITALICMVVSIIIYIPFVRIATKKDQKN
HHCCCCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TVEVEKTHSI
CEEEHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8990303; 8969509; 9384377 [H]