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The map label for this gene is miaD [H]

Identifier: 148381272

GI number: 148381272

Start: 3546922

End: 3548511

Strand: Reverse

Name: miaD [H]

Synonym: CBO3329

Alternate gene names: 148381272

Gene position: 3548511-3546922 (Counterclockwise)

Preceding gene: 148381274

Following gene: 148381270

Centisome position: 91.29

GC content: 32.08

Gene sequence:

>1590_bases
ATGGCTGATTTAAGAGAAAAAATAGAAACAAGAAGAACCTTTGCTATAATATCACATCCAGATGCTGGTAAGACTACTTT
AACAGAAAAGCTATTATTATACGGAGGAGCTATAAGGCTAGCAGGTTCTGTAAAAGCTAGAAAGGCGTCAAGACATGCCA
CTTCAGACTGGATGGAAATAGAAAAACAAAGAGGAATATCAGTAACTTCATCTGTAATGCAGTTTAATTATGAGGGATAT
TGTATAAACATATTAGATACTCCAGGACATCAAGATTTTAGTGAAGATACATATAGAACTTTAATGGCAGCAGACAGTGC
GGTAATGGTTATAGATGCTGCCAAAGGTGTAGAAGAACAAACAAAAAAATTATTCCATGTATGTAGTTTAAGAGGAATCC
CTATATTTACCTTTGTAAATAAAATGGATAGAGAAAGTAAAGATCCCTTTGAACTTATGGAGGATATAGAAAGTGTATTA
GGTATAAAATCTTACCCAATAAACTGGCCTATAGGATCAGGTAAAGGCTTTAAAGGAGTTTATGACAGAAATAAAAAGAT
AATTCAAGCCTTTAATGGTGGAAACCATGGTCAAACAGCAGTAGAATCCTTATCAGGGGATATAGATGATGATGTATTTA
AAGATTTAATAGGTGAAGATTTACATGAAAAATTAAAAGAGGATATAGAACTTTTAGACATTGCAGGAGATGATTTTGAT
TTAGAAAAGGTAAGAGTCGGCGAACTTACACCAGTATTTTTTGGTAGTGCTTTAACCAACTTTGGAGTGGAACCATTTTT
AAAAGAGTTTTTAAATTTAACACAACCACCGTTATCAAGGGAATCAGATATTGGAGAAATAGATGTATTTAGTGATAAAT
TTTCTGCCTTTATATTTAAGATTCAAGCTAATATGAATCCAGCCCATAGGGATAGAATAGCTTTTATGAGGATATGTTCT
GGTAAGTTTAAAAAAGGCATGGAAGTTTACCATTATCAAGGCGGAAACAAAGTTAAGCTTGCTCAACCACAACAGTTTTT
AGCCCAAGATAGGGAAATAGTTGATGAAGCTTATGCAGGAGATATAATTGGAGTGTTTGATCCAGGCATATTTAGAATAG
GAGACACTCTATGTTCTGCTCAAGATAAATTTAGATTTGAAGGTATACCTACTTTTGCTCCAGAGTATTTTGCTAGAGTT
AGAACTATAGATACTATGAAAAGAAAACAATTTATAAAGGGCATAACTCAAATATCTCAAGAAGGGGCAATACAAGTATT
TAAAGAACTTCATATAGGAATAGAAGAAATAGTAGTTGGAGTAGTAGGTGTTCTTCAGTTTGAAGTTTTAGAATATAGAA
TGAAAAATGAATATAATGTAGATATAAAATTAGAAAGAGTACCTTATAAATATGTAAGATGGATTGAGAAAACAGAAAAA
GAAGCAGAAAAATTAAGTATAACTAGTGATACAAAGATAGTAAAAGATTTAAAAGATAGAGATTTACTATTATTCCAAAG
TGATTGGGCTATAAGCTGGGCTTTGGAGCATAATGAGGGATTAGTACTTTCAGATATAGGTAAGAATTAA

Upstream 100 bases:

>100_bases
CAATATTTACAAAAATTATTCTTATATTATTTTGTAGGTGGAGTTTCAATGTTAATTGGCTAGAGAATAAATATTTATTG
CAAAAAAGGAGAGTGATTTA

Downstream 100 bases:

>100_bases
ATTTAAATAGTTAAATATCTAAGTTAATATTTTTAAATAATAAAAAGAAATGAGACTTAAATTTAAGCCTCATTTCTTTT
GTTTTAGATTAATATAGTGA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MADLREKIETRRTFAIISHPDAGKTTLTEKLLLYGGAIRLAGSVKARKASRHATSDWMEIEKQRGISVTSSVMQFNYEGY
CINILDTPGHQDFSEDTYRTLMAADSAVMVIDAAKGVEEQTKKLFHVCSLRGIPIFTFVNKMDRESKDPFELMEDIESVL
GIKSYPINWPIGSGKGFKGVYDRNKKIIQAFNGGNHGQTAVESLSGDIDDDVFKDLIGEDLHEKLKEDIELLDIAGDDFD
LEKVRVGELTPVFFGSALTNFGVEPFLKEFLNLTQPPLSRESDIGEIDVFSDKFSAFIFKIQANMNPAHRDRIAFMRICS
GKFKKGMEVYHYQGGNKVKLAQPQQFLAQDREIVDEAYAGDIIGVFDPGIFRIGDTLCSAQDKFRFEGIPTFAPEYFARV
RTIDTMKRKQFIKGITQISQEGAIQVFKELHIGIEEIVVGVVGVLQFEVLEYRMKNEYNVDIKLERVPYKYVRWIEKTEK
EAEKLSITSDTKIVKDLKDRDLLLFQSDWAISWALEHNEGLVLSDIGKN

Sequences:

>Translated_529_residues
MADLREKIETRRTFAIISHPDAGKTTLTEKLLLYGGAIRLAGSVKARKASRHATSDWMEIEKQRGISVTSSVMQFNYEGY
CINILDTPGHQDFSEDTYRTLMAADSAVMVIDAAKGVEEQTKKLFHVCSLRGIPIFTFVNKMDRESKDPFELMEDIESVL
GIKSYPINWPIGSGKGFKGVYDRNKKIIQAFNGGNHGQTAVESLSGDIDDDVFKDLIGEDLHEKLKEDIELLDIAGDDFD
LEKVRVGELTPVFFGSALTNFGVEPFLKEFLNLTQPPLSRESDIGEIDVFSDKFSAFIFKIQANMNPAHRDRIAFMRICS
GKFKKGMEVYHYQGGNKVKLAQPQQFLAQDREIVDEAYAGDIIGVFDPGIFRIGDTLCSAQDKFRFEGIPTFAPEYFARV
RTIDTMKRKQFIKGITQISQEGAIQVFKELHIGIEEIVVGVVGVLQFEVLEYRMKNEYNVDIKLERVPYKYVRWIEKTEK
EAEKLSITSDTKIVKDLKDRDLLLFQSDWAISWALEHNEGLVLSDIGKN
>Mature_528_residues
ADLREKIETRRTFAIISHPDAGKTTLTEKLLLYGGAIRLAGSVKARKASRHATSDWMEIEKQRGISVTSSVMQFNYEGYC
INILDTPGHQDFSEDTYRTLMAADSAVMVIDAAKGVEEQTKKLFHVCSLRGIPIFTFVNKMDRESKDPFELMEDIESVLG
IKSYPINWPIGSGKGFKGVYDRNKKIIQAFNGGNHGQTAVESLSGDIDDDVFKDLIGEDLHEKLKEDIELLDIAGDDFDL
EKVRVGELTPVFFGSALTNFGVEPFLKEFLNLTQPPLSRESDIGEIDVFSDKFSAFIFKIQANMNPAHRDRIAFMRICSG
KFKKGMEVYHYQGGNKVKLAQPQQFLAQDREIVDEAYAGDIIGVFDPGIFRIGDTLCSAQDKFRFEGIPTFAPEYFARVR
TIDTMKRKQFIKGITQISQEGAIQVFKELHIGIEEIVVGVVGVLQFEVLEYRMKNEYNVDIKLERVPYKYVRWIEKTEKE
AEKLSITSDTKIVKDLKDRDLLLFQSDWAISWALEHNEGLVLSDIGKN

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=491, Percent_Identity=29.3279022403259, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI19923640, Length=527, Percent_Identity=25.426944971537, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI25306283, Length=376, Percent_Identity=29.5212765957447, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI25306287, Length=281, Percent_Identity=32.7402135231317, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI157426893, Length=164, Percent_Identity=33.5365853658537, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI94966754, Length=150, Percent_Identity=34, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=30.8724832214765, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI310132016, Length=129, Percent_Identity=34.1085271317829, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI310110807, Length=129, Percent_Identity=34.1085271317829, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI310123363, Length=129, Percent_Identity=34.1085271317829, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI1790835, Length=472, Percent_Identity=50.2118644067797, Blast_Score=487, Evalue=1e-138,
Organism=Escherichia coli, GI1789738, Length=501, Percent_Identity=27.7445109780439, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1788922, Length=178, Percent_Identity=32.0224719101124, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI48994988, Length=142, Percent_Identity=35.2112676056338, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=497, Percent_Identity=28.5714285714286, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17556745, Length=492, Percent_Identity=24.1869918699187, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17557151, Length=137, Percent_Identity=32.8467153284672, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=31.5789473684211, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=31.5789473684211, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=31.6770186335404, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=488, Percent_Identity=27.8688524590164, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6322359, Length=533, Percent_Identity=23.4521575984991, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323320, Length=173, Percent_Identity=28.3236994219653, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324707, Length=160, Percent_Identity=30, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=160, Percent_Identity=30, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322937, Length=212, Percent_Identity=27.3584905660377, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24582462, Length=494, Percent_Identity=30.1619433198381, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI221458488, Length=544, Percent_Identity=25.5514705882353, Blast_Score=135, Evalue=1e-31,
Organism=Drosophila melanogaster, GI78706572, Length=160, Percent_Identity=36.25, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24585709, Length=227, Percent_Identity=26.8722466960352, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24585711, Length=219, Percent_Identity=26.9406392694064, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24585713, Length=219, Percent_Identity=26.9406392694064, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21357743, Length=177, Percent_Identity=29.9435028248588, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59958; Mature: 59827

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLREKIETRRTFAIISHPDAGKTTLTEKLLLYGGAIRLAGSVKARKASRHATSDWMEI
CCHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHCCHHHHHHH
EKQRGISVTSSVMQFNYEGYCINILDTPGHQDFSEDTYRTLMAADSAVMVIDAAKGVEEQ
HHHCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCHHHH
TKKLFHVCSLRGIPIFTFVNKMDRESKDPFELMEDIESVLGIKSYPINWPIGSGKGFKGV
HHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCH
YDRNKKIIQAFNGGNHGQTAVESLSGDIDDDVFKDLIGEDLHEKLKEDIELLDIAGDDFD
HHCCCHHEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
LEKVRVGELTPVFFGSALTNFGVEPFLKEFLNLTQPPLSRESDIGEIDVFSDKFSAFIFK
CCEEEECCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHEEEEEE
IQANMNPAHRDRIAFMRICSGKFKKGMEVYHYQGGNKVKLAQPQQFLAQDREIVDEAYAG
EECCCCHHHHHHHHHHHHHCHHHHCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHCC
DIIGVFDPGIFRIGDTLCSAQDKFRFEGIPTFAPEYFARVRTIDTMKRKQFIKGITQISQ
CEEEECCCCHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGAIQVFKELHIGIEEIVVGVVGVLQFEVLEYRMKNEYNVDIKLERVPYKYVRWIEKTEK
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHH
EAEKLSITSDTKIVKDLKDRDLLLFQSDWAISWALEHNEGLVLSDIGKN
HHHHEECCCCHHHHHHCCCCCEEEEECCCEEEEEEECCCCEEECCCCCC
>Mature Secondary Structure 
ADLREKIETRRTFAIISHPDAGKTTLTEKLLLYGGAIRLAGSVKARKASRHATSDWMEI
CHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHCCHHHHHHH
EKQRGISVTSSVMQFNYEGYCINILDTPGHQDFSEDTYRTLMAADSAVMVIDAAKGVEEQ
HHHCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCHHHH
TKKLFHVCSLRGIPIFTFVNKMDRESKDPFELMEDIESVLGIKSYPINWPIGSGKGFKGV
HHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCH
YDRNKKIIQAFNGGNHGQTAVESLSGDIDDDVFKDLIGEDLHEKLKEDIELLDIAGDDFD
HHCCCHHEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
LEKVRVGELTPVFFGSALTNFGVEPFLKEFLNLTQPPLSRESDIGEIDVFSDKFSAFIFK
CCEEEECCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHEEEEEE
IQANMNPAHRDRIAFMRICSGKFKKGMEVYHYQGGNKVKLAQPQQFLAQDREIVDEAYAG
EECCCCHHHHHHHHHHHHHCHHHHCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHCC
DIIGVFDPGIFRIGDTLCSAQDKFRFEGIPTFAPEYFARVRTIDTMKRKQFIKGITQISQ
CEEEECCCCHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGAIQVFKELHIGIEEIVVGVVGVLQFEVLEYRMKNEYNVDIKLERVPYKYVRWIEKTEK
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHH
EAEKLSITSDTKIVKDLKDRDLLLFQSDWAISWALEHNEGLVLSDIGKN
HHHHEECCCCHHHHHHCCCCCEEEEECCCEEEEEEECCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA