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The map label for this gene is oppA [H]

Identifier: 148381209

GI number: 148381209

Start: 3467515

End: 3469077

Strand: Reverse

Name: oppA [H]

Synonym: CBO3264

Alternate gene names: 148381209

Gene position: 3469077-3467515 (Counterclockwise)

Preceding gene: 148381210

Following gene: 148381206

Centisome position: 89.25

GC content: 24.63

Gene sequence:

>1563_bases
ATGAAAAAGATTATATGTTTTTTATTAAGTTTTACATTAATTTTTTTTAGCGGTTGTGTAGAAAAAAACAAAGAAGTCAC
CTCAGATGAGGGAATAAGAGATTACTTAATATATAATATAGAAACTATGCCTAAGGATTTAATTATGCTAGAAGACGGTT
CACAAAGAGAGAAGGACTTATTATTAAGTCTATTTGATGGATTGGTAGGGTTAGATAAAAATAATAACATTGTTCCGGAA
TTGGCAGAAAAATGGGAAGTTAGTAAAGATGGTATAGATTATAAATTTTATATTAAGGATAATTTATTTTGGAGCAATGG
GAAGGAAATAGTAGCTAGGGATTTTGAAAAATTTTTTAAAGATATTCTTATATATTGTAAGGAAAATAATATAAGATTCA
ATGAACTAAATTCTGTATATGGAGTTAAGGACTACTTAACTAATGGAAATCTAGATAACATAGCTATAAAAGCTAAAGAA
GATAATATATTAGAAATAAGACTAAATAATAAAGATCCATATTTTTTAAGTACTCTTTCTAAGCCTAAATATAAATTAAG
AAAAATAGATAAGAAAAACAAAAATTATAAAAAAAGTTTTAATGAAATACCATATACAGGAGCATTTTGTATAAATAAAA
TAGAGAAAGACCAAATAACAATTAACAAAAATAATAAATATTGGGATAAAGAAAATATAAAGATTAATAGAATAGTGCTT
AAATCTATACAAAACAGTGAAGAGGCATTGGCTCATTTAAAGACTTCCAAATTAGACTTGTTTATGAATCCTCCTATAGC
TGAAATTAATAATTTAAATAAAGAAGGATTAATATCTAAAAAGTTATCTTTAGAAACAGGGAATATAATATTCAATAAAA
AAGATTATATAGATCTAAACTTAAGAAGGGCAATAGATAGCTGTATTAGTAGGGAGGATATGTGTTCGAAGTTACTATAT
GGGAAATCAGTGCCAGCCATTGCTTATGTACCAAATAATATAGCAGAAAATGATGATATAAGTTATGGTAAAAATTATTT
TAGTTTAAATAAAAATTTACAAAGGGCAAAAGAATTTTTAGATAAAAGTGAATATAAAAAAGAAAAGAAGGATTTAAAAA
TTATATCAATAGAAGATCCTATTTCTAATAAAATAGCTAATAGTATAATAGGGGAATTAAAAGAGAGTTTAGATATAAAT
CTTAATTTGGTTAATTGCAAGGATGAGAATGAATTAGAACAGAAAATTAAAAATGGAGATTATGACTTAGCCTTTGTTAG
AAATAAAGCTAAAAATAACGATCCTTTATTTTTCCTCTTAAACTTTACTAGTGATTCTTTGCAATACCAAGATTTTTTAA
ATAAAATTAAATTAGAAGATAATATGTGGAAAAAGAGAGAATATATCAATAAAGCACAAGATGTTCTGGCTAAGGATTTG
TATACAATACCTGTTTGTTTTTTTTATGATATGTTATGTAAAAGAAGTAGGGTACAAGGGCAATATATTACCATAGATGG
TAATGTTATTATAAAGAAAATAACCTTAGCACCCTATAATTAA

Upstream 100 bases:

>100_bases
TAGTTTGAATTATATGTAAAGTTTAAAAATGTTGTTTATTTTAACCTATAAGTGTAAATAATTAAATATAGTATATGATT
CTTCAAAGCGGGGGGATAAA

Downstream 100 bases:

>100_bases
TATTATTTTATAGTGGTTTTATTTTTAGATGATTTAATAGGAAGAAAATAAGCACAATTAGCGTTTTCTTCGCGAATACT
TAAAAAGGGACTGATGTAAT

Product: extracellular solute-binding protein, family 5

Products: NA

Alternate protein names: Stage 0 sporulation protein KA [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MKKIICFLLSFTLIFFSGCVEKNKEVTSDEGIRDYLIYNIETMPKDLIMLEDGSQREKDLLLSLFDGLVGLDKNNNIVPE
LAEKWEVSKDGIDYKFYIKDNLFWSNGKEIVARDFEKFFKDILIYCKENNIRFNELNSVYGVKDYLTNGNLDNIAIKAKE
DNILEIRLNNKDPYFLSTLSKPKYKLRKIDKKNKNYKKSFNEIPYTGAFCINKIEKDQITINKNNKYWDKENIKINRIVL
KSIQNSEEALAHLKTSKLDLFMNPPIAEINNLNKEGLISKKLSLETGNIIFNKKDYIDLNLRRAIDSCISREDMCSKLLY
GKSVPAIAYVPNNIAENDDISYGKNYFSLNKNLQRAKEFLDKSEYKKEKKDLKIISIEDPISNKIANSIIGELKESLDIN
LNLVNCKDENELEQKIKNGDYDLAFVRNKAKNNDPLFFLLNFTSDSLQYQDFLNKIKLEDNMWKKREYINKAQDVLAKDL
YTIPVCFFYDMLCKRSRVQGQYITIDGNVIIKKITLAPYN

Sequences:

>Translated_520_residues
MKKIICFLLSFTLIFFSGCVEKNKEVTSDEGIRDYLIYNIETMPKDLIMLEDGSQREKDLLLSLFDGLVGLDKNNNIVPE
LAEKWEVSKDGIDYKFYIKDNLFWSNGKEIVARDFEKFFKDILIYCKENNIRFNELNSVYGVKDYLTNGNLDNIAIKAKE
DNILEIRLNNKDPYFLSTLSKPKYKLRKIDKKNKNYKKSFNEIPYTGAFCINKIEKDQITINKNNKYWDKENIKINRIVL
KSIQNSEEALAHLKTSKLDLFMNPPIAEINNLNKEGLISKKLSLETGNIIFNKKDYIDLNLRRAIDSCISREDMCSKLLY
GKSVPAIAYVPNNIAENDDISYGKNYFSLNKNLQRAKEFLDKSEYKKEKKDLKIISIEDPISNKIANSIIGELKESLDIN
LNLVNCKDENELEQKIKNGDYDLAFVRNKAKNNDPLFFLLNFTSDSLQYQDFLNKIKLEDNMWKKREYINKAQDVLAKDL
YTIPVCFFYDMLCKRSRVQGQYITIDGNVIIKKITLAPYN
>Mature_520_residues
MKKIICFLLSFTLIFFSGCVEKNKEVTSDEGIRDYLIYNIETMPKDLIMLEDGSQREKDLLLSLFDGLVGLDKNNNIVPE
LAEKWEVSKDGIDYKFYIKDNLFWSNGKEIVARDFEKFFKDILIYCKENNIRFNELNSVYGVKDYLTNGNLDNIAIKAKE
DNILEIRLNNKDPYFLSTLSKPKYKLRKIDKKNKNYKKSFNEIPYTGAFCINKIEKDQITINKNNKYWDKENIKINRIVL
KSIQNSEEALAHLKTSKLDLFMNPPIAEINNLNKEGLISKKLSLETGNIIFNKKDYIDLNLRRAIDSCISREDMCSKLLY
GKSVPAIAYVPNNIAENDDISYGKNYFSLNKNLQRAKEFLDKSEYKKEKKDLKIISIEDPISNKIANSIIGELKESLDIN
LNLVNCKDENELEQKIKNGDYDLAFVRNKAKNNDPLFFLLNFTSDSLQYQDFLNKIKLEDNMWKKREYINKAQDVLAKDL
YTIPVCFFYDMLCKRSRVQGQYITIDGNVIIKKITLAPYN

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, It binds peptides up to five amino acids long with high affinity. Also required for sporulation and competence [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789397, Length=506, Percent_Identity=22.7272727272727, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787495, Length=480, Percent_Identity=21.6666666666667, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI87081878, Length=456, Percent_Identity=21.9298245614035, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789966, Length=437, Percent_Identity=21.2814645308924, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60657; Mature: 60657

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIICFLLSFTLIFFSGCVEKNKEVTSDEGIRDYLIYNIETMPKDLIMLEDGSQREKDL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCEEEEECCCCCHHHHH
LLSLFDGLVGLDKNNNIVPELAEKWEVSKDGIDYKFYIKDNLFWSNGKEIVARDFEKFFK
HHHHHHHHEECCCCCCCCHHHHHHCCCCCCCCCEEEEEECCEEECCCHHHHHHHHHHHHH
DILIYCKENNIRFNELNSVYGVKDYLTNGNLDNIAIKAKEDNILEIRLNNKDPYFLSTLS
HHEEEECCCCEEHHHHCCHHCHHHHHCCCCCCCEEEEECCCCEEEEEECCCCCEEEECCC
KPKYKLRKIDKKNKNYKKSFNEIPYTGAFCINKIEKDQITINKNNKYWDKENIKINRIVL
CCHHHHHHHHHCCCCHHHHHHCCCCCHHHHHCCCCCCEEEEECCCCCCCCCCCEEHHHHH
KSIQNSEEALAHLKTSKLDLFMNPPIAEINNLNKEGLISKKLSLETGNIIFNKKDYIDLN
HHHCCHHHHHHHHHHCCEEEEECCCHHHHHCCCCCCCEEHEEEEECCCEEECCCCEEEHH
LRRAIDSCISREDMCSKLLYGKSVPAIAYVPNNIAENDDISYGKNYFSLNKNLQRAKEFL
HHHHHHHHHCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCHHHHCHHHHHHHHHH
DKSEYKKEKKDLKIISIEDPISNKIANSIIGELKESLDINLNLVNCKDENELEQKIKNGD
HHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCC
YDLAFVRNKAKNNDPLFFLLNFTSDSLQYQDFLNKIKLEDNMWKKREYINKAQDVLAKDL
CEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
YTIPVCFFYDMLCKRSRVQGQYITIDGNVIIKKITLAPYN
HHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEECCCC
>Mature Secondary Structure
MKKIICFLLSFTLIFFSGCVEKNKEVTSDEGIRDYLIYNIETMPKDLIMLEDGSQREKDL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCEEEEECCCCCHHHHH
LLSLFDGLVGLDKNNNIVPELAEKWEVSKDGIDYKFYIKDNLFWSNGKEIVARDFEKFFK
HHHHHHHHEECCCCCCCCHHHHHHCCCCCCCCCEEEEEECCEEECCCHHHHHHHHHHHHH
DILIYCKENNIRFNELNSVYGVKDYLTNGNLDNIAIKAKEDNILEIRLNNKDPYFLSTLS
HHEEEECCCCEEHHHHCCHHCHHHHHCCCCCCCEEEEECCCCEEEEEECCCCCEEEECCC
KPKYKLRKIDKKNKNYKKSFNEIPYTGAFCINKIEKDQITINKNNKYWDKENIKINRIVL
CCHHHHHHHHHCCCCHHHHHHCCCCCHHHHHCCCCCCEEEEECCCCCCCCCCCEEHHHHH
KSIQNSEEALAHLKTSKLDLFMNPPIAEINNLNKEGLISKKLSLETGNIIFNKKDYIDLN
HHHCCHHHHHHHHHHCCEEEEECCCHHHHHCCCCCCCEEHEEEEECCCEEECCCCEEEHH
LRRAIDSCISREDMCSKLLYGKSVPAIAYVPNNIAENDDISYGKNYFSLNKNLQRAKEFL
HHHHHHHHHCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCHHHHCHHHHHHHHHH
DKSEYKKEKKDLKIISIEDPISNKIANSIIGELKESLDINLNLVNCKDENELEQKIKNGD
HHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCC
YDLAFVRNKAKNNDPLFFLLNFTSDSLQYQDFLNKIKLEDNMWKKREYINKAQDVLAKDL
CEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
YTIPVCFFYDMLCKRSRVQGQYITIDGNVIIKKITLAPYN
HHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]