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The map label for this gene is pflD [H]

Identifier: 148381131

GI number: 148381131

Start: 3388555

End: 3390918

Strand: Reverse

Name: pflD [H]

Synonym: CBO3183

Alternate gene names: 148381131

Gene position: 3390918-3388555 (Counterclockwise)

Preceding gene: 148381132

Following gene: 148381130

Centisome position: 87.24

GC content: 31.18

Gene sequence:

>2364_bases
ATGATGACAGACAGAGTTAAAAAATTAAGAGAAGAAAGTTTAAAGGCTGTACCACGTATTTCTATGGAGAGAACTAGAAT
AGTTACTGATGTTTATAAAAAATATGAAGGAACAGTATCAATTCCAGTATTAAGAGCATTAGTTTTAAAGGAATTAATGG
AAAGAAAAGAGCTTTGTATTTATGATGGAGAACTTATAGTAGGTGAAAGAGGGGAAGCTGCAGCAGCTACACCAACTTAT
CCAGAATTATGCTGTCATACAGTTGAAGACTTTGATATAATGGATAAACGTGAAAAGATATCCTTTAAAACAACTGATGA
AGATAAAGAAATACAACAAGAATTAATTATTCCTTTCTGGGAAAAAAGATCTATGAGACATAAAATATTAGAAAAAATGA
CTCCAGAATGGAAAGCTTGTTATGAAGCAGGTATATTCACCGAATTTATGGAACAAAGGGGACCAGGGCATACTGCAGGT
GGAGATAAATATTATAAAATGGGATTTTTAGATATTAAAGAACAAATAAAAGAAGCTATAAGTAAATTAGATTATTTAAA
TGATGATGAAGCATTAGATAAAAAAGAACAATTAGATGCTATGGATATAGCTTGTGATGCTATAATGATTTATGGAAAAC
GTTATTCAGAATATGCGGCCAAATTAGCACAAAAAGAAGCAGATCCAGTAAGAAAAAAAGAACTTATGGAAATATCTGAA
GTTTGTAGTTGGGTACCAGCTCATGCTCCAAGAACCTTTAGAGAAGCAATTCAAATGTACTGGTTTGTTCATTTATGTGT
AATTTCTGAATTAAATCCTTGGGATGCATTTAATCCTGGTAGATTAGATCAACATTTATATCCGTTTTATAAAAAACAAA
TAGAAGAGGGAACATTAGATAGGGAGCAGGCTAGAGAATTGTTGCAATGTTTCTGGGTTAAATTTAATAACCAACCAGCG
CCACCAAAAGTTGGTATAACTTTAAAAGAAAGTGGAACATATACTGACTTTGCAAACATAAATAGTGGTGGTATGAAAGC
TGATGGATCAGATGGTGTTAATGATGTAAGCTATTTAGTACTTGAAGTAATAGATGAAATGAAGTTATTGCAACCAAGTT
CTAATGTACAGATAAGTAAAAAAACCCCTCAAAGATTTTTAAAGAAGGCTTGCGAAGTTATAAGAAAAGGATGGGGCCAA
CCATCAATATTTAATGCTGACTCAGTAGTTCAAGAATTAGTAAGAGCAGGAAAATCTATAGAAGATGCTAGATGCGGAGG
AACTAGTGGTTGTGTTGAAGCTGGAGCTTTTGGTAAAGAAGCTTATATATTAACTGGATACTTTAATTTACCAAAAATAT
TAGAAATAACATTATTAAATGGTGTGGATTCTCAAACTGGTAAACAATTAGGTATAAAAACAGGAGATATAAGTACATTA
AAAACTTATGAAGATTTACTAGATGCATTTAAAAAACAACTTAAATATTTTGTTGATATAAAAGTAAATGGTAATAGAGT
TATAGAAAGGTTATATGCAACATTGATGCCTGCACCATTCTTATCAGTTGTTACAGATGATTGTATAGCAAAAGGAAAAG
ATTATAATGCAGGAGGAGCAAGATATAATACTAGTTATATTCAAGGTGTTGGTATAGGGACAATTACAGACAGTTTATCA
GCAATCAAATACCAAGTATTTGATGAAAAGAATATAACAATGGAAGAATTAATGGAAGCATTAAGATTTAATTTTGAAGG
ACATGAAGATATATATAACTTAGTTAAGAATAAAACTCCTAAATATGGTAATGATGATGACTATGCAGATGAAATAATGA
AGGAAGTATTTGATGCTTATTATAATGAAGTAAATGGAAGACCTAATGGTAGAGAAGGATGCTATAGAATAGATATGCTA
CCAACAACATGCCATGTTTACTTTGGATCAGTTATAAATGCTACTCCAGATGGAAGAAAAGCTCATATTCCAGTATCAGA
AGGTATTTCTCCATCAAAGGGTGCAGATGTAAATGGACCAACAGGCGTTATAAAGTCAGCAGCTAAAATGGATCATTTAA
GAACTGGTGGTACTTTATTGAATCAAAAATTTGTTCCTTCAGTTGTTCAAGGTGAAGAAGGAATAGATAATATGGCTAAT
TTAGTAAGAGGTTATTTTACAATGGACGGGCATCACATTCAATTTAATATTGTAAGTAAGGAAACATTATTAAAAGCTCA
ACAAAATCCGGATGAATATAAAGATTTAATAGTTCGTGTTGCTGGATATAGCGACTACTTTAATAACTTAGATAAAGTTT
TACAAAATGAGATAATAGAAAGAACAGAACAAGAGTTTAATTAA

Upstream 100 bases:

>100_bases
ATTAACTGGTGAGGAAAAACCATCAGATGAGAAAATGAATGAGATAGCAGAAAAATTTAAGCAGGCTGGAATTAAAGTAA
AAATAGGGGGGTAATTAATT

Downstream 100 bases:

>100_bases
AAACTAGTTTAATATATTATTGTAAAGAAAAATAAAAACTAGGAGAAGGAACTAAGATTTATAATTTAAGTCTTTCTCCT
AGTAACAAATTTTTATTAGG

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 2 [H]

Number of amino acids: Translated: 787; Mature: 787

Protein sequence:

>787_residues
MMTDRVKKLREESLKAVPRISMERTRIVTDVYKKYEGTVSIPVLRALVLKELMERKELCIYDGELIVGERGEAAAATPTY
PELCCHTVEDFDIMDKREKISFKTTDEDKEIQQELIIPFWEKRSMRHKILEKMTPEWKACYEAGIFTEFMEQRGPGHTAG
GDKYYKMGFLDIKEQIKEAISKLDYLNDDEALDKKEQLDAMDIACDAIMIYGKRYSEYAAKLAQKEADPVRKKELMEISE
VCSWVPAHAPRTFREAIQMYWFVHLCVISELNPWDAFNPGRLDQHLYPFYKKQIEEGTLDREQARELLQCFWVKFNNQPA
PPKVGITLKESGTYTDFANINSGGMKADGSDGVNDVSYLVLEVIDEMKLLQPSSNVQISKKTPQRFLKKACEVIRKGWGQ
PSIFNADSVVQELVRAGKSIEDARCGGTSGCVEAGAFGKEAYILTGYFNLPKILEITLLNGVDSQTGKQLGIKTGDISTL
KTYEDLLDAFKKQLKYFVDIKVNGNRVIERLYATLMPAPFLSVVTDDCIAKGKDYNAGGARYNTSYIQGVGIGTITDSLS
AIKYQVFDEKNITMEELMEALRFNFEGHEDIYNLVKNKTPKYGNDDDYADEIMKEVFDAYYNEVNGRPNGREGCYRIDML
PTTCHVYFGSVINATPDGRKAHIPVSEGISPSKGADVNGPTGVIKSAAKMDHLRTGGTLLNQKFVPSVVQGEEGIDNMAN
LVRGYFTMDGHHIQFNIVSKETLLKAQQNPDEYKDLIVRVAGYSDYFNNLDKVLQNEIIERTEQEFN

Sequences:

>Translated_787_residues
MMTDRVKKLREESLKAVPRISMERTRIVTDVYKKYEGTVSIPVLRALVLKELMERKELCIYDGELIVGERGEAAAATPTY
PELCCHTVEDFDIMDKREKISFKTTDEDKEIQQELIIPFWEKRSMRHKILEKMTPEWKACYEAGIFTEFMEQRGPGHTAG
GDKYYKMGFLDIKEQIKEAISKLDYLNDDEALDKKEQLDAMDIACDAIMIYGKRYSEYAAKLAQKEADPVRKKELMEISE
VCSWVPAHAPRTFREAIQMYWFVHLCVISELNPWDAFNPGRLDQHLYPFYKKQIEEGTLDREQARELLQCFWVKFNNQPA
PPKVGITLKESGTYTDFANINSGGMKADGSDGVNDVSYLVLEVIDEMKLLQPSSNVQISKKTPQRFLKKACEVIRKGWGQ
PSIFNADSVVQELVRAGKSIEDARCGGTSGCVEAGAFGKEAYILTGYFNLPKILEITLLNGVDSQTGKQLGIKTGDISTL
KTYEDLLDAFKKQLKYFVDIKVNGNRVIERLYATLMPAPFLSVVTDDCIAKGKDYNAGGARYNTSYIQGVGIGTITDSLS
AIKYQVFDEKNITMEELMEALRFNFEGHEDIYNLVKNKTPKYGNDDDYADEIMKEVFDAYYNEVNGRPNGREGCYRIDML
PTTCHVYFGSVINATPDGRKAHIPVSEGISPSKGADVNGPTGVIKSAAKMDHLRTGGTLLNQKFVPSVVQGEEGIDNMAN
LVRGYFTMDGHHIQFNIVSKETLLKAQQNPDEYKDLIVRVAGYSDYFNNLDKVLQNEIIERTEQEFN
>Mature_787_residues
MMTDRVKKLREESLKAVPRISMERTRIVTDVYKKYEGTVSIPVLRALVLKELMERKELCIYDGELIVGERGEAAAATPTY
PELCCHTVEDFDIMDKREKISFKTTDEDKEIQQELIIPFWEKRSMRHKILEKMTPEWKACYEAGIFTEFMEQRGPGHTAG
GDKYYKMGFLDIKEQIKEAISKLDYLNDDEALDKKEQLDAMDIACDAIMIYGKRYSEYAAKLAQKEADPVRKKELMEISE
VCSWVPAHAPRTFREAIQMYWFVHLCVISELNPWDAFNPGRLDQHLYPFYKKQIEEGTLDREQARELLQCFWVKFNNQPA
PPKVGITLKESGTYTDFANINSGGMKADGSDGVNDVSYLVLEVIDEMKLLQPSSNVQISKKTPQRFLKKACEVIRKGWGQ
PSIFNADSVVQELVRAGKSIEDARCGGTSGCVEAGAFGKEAYILTGYFNLPKILEITLLNGVDSQTGKQLGIKTGDISTL
KTYEDLLDAFKKQLKYFVDIKVNGNRVIERLYATLMPAPFLSVVTDDCIAKGKDYNAGGARYNTSYIQGVGIGTITDSLS
AIKYQVFDEKNITMEELMEALRFNFEGHEDIYNLVKNKTPKYGNDDDYADEIMKEVFDAYYNEVNGRPNGREGCYRIDML
PTTCHVYFGSVINATPDGRKAHIPVSEGISPSKGADVNGPTGVIKSAAKMDHLRTGGTLLNQKFVPSVVQGEEGIDNMAN
LVRGYFTMDGHHIQFNIVSKETLLKAQQNPDEYKDLIVRVAGYSDYFNNLDKVLQNEIIERTEQEFN

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1790388, Length=801, Percent_Identity=33.458177278402, Blast_Score=423, Evalue=1e-119,
Organism=Escherichia coli, GI1787044, Length=811, Percent_Identity=31.9358816276202, Blast_Score=404, Evalue=1e-113,
Organism=Escherichia coli, GI48994926, Length=699, Percent_Identity=23.4620886981402, Blast_Score=152, Evalue=8e-38,
Organism=Escherichia coli, GI1787131, Length=684, Percent_Identity=22.3684210526316, Blast_Score=130, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 89158; Mature: 89158

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTDRVKKLREESLKAVPRISMERTRIVTDVYKKYEGTVSIPVLRALVLKELMERKELCI
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEE
YDGELIVGERGEAAAATPTYPELCCHTVEDFDIMDKREKISFKTTDEDKEIQQELIIPFW
ECCEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHCCHHHCCCCCCCCHHHHHHHHCCCHH
EKRSMRHKILEKMTPEWKACYEAGIFTEFMEQRGPGHTAGGDKYYKMGFLDIKEQIKEAI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHH
SKLDYLNDDEALDKKEQLDAMDIACDAIMIYGKRYSEYAAKLAQKEADPVRKKELMEISE
HHHCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VCSWVPAHAPRTFREAIQMYWFVHLCVISELNPWDAFNPGRLDQHLYPFYKKQIEEGTLD
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
REQARELLQCFWVKFNNQPAPPKVGITLKESGTYTDFANINSGGMKADGSDGVNDVSYLV
HHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LEVIDEMKLLQPSSNVQISKKTPQRFLKKACEVIRKGWGQPSIFNADSVVQELVRAGKSI
HHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCC
EDARCGGTSGCVEAGAFGKEAYILTGYFNLPKILEITLLNGVDSQTGKQLGIKTGDISTL
CHHCCCCCCCHHHCCCCCCCEEEEEECCCCCHHEEEEEECCCCCCCCCCCCCCCCCCHHH
KTYEDLLDAFKKQLKYFVDIKVNGNRVIERLYATLMPAPFLSVVTDDCIAKGKDYNAGGA
HHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC
RYNTSYIQGVGIGTITDSLSAIKYQVFDEKNITMEELMEALRFNFEGHEDIYNLVKNKTP
EECCHHHCCCCCHHHHHHHHHHEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCC
KYGNDDDYADEIMKEVFDAYYNEVNGRPNGREGCYRIDMLPTTCHVYFGSVINATPDGRK
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCE
AHIPVSEGISPSKGADVNGPTGVIKSAAKMDHLRTGGTLLNQKFVPSVVQGEEGIDNMAN
EECCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCHHHCCCCCHHHHHH
LVRGYFTMDGHHIQFNIVSKETLLKAQQNPDEYKDLIVRVAGYSDYFNNLDKVLQNEIIE
HHHHHHEECCCEEEEEEECHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RTEQEFN
HHHHHCC
>Mature Secondary Structure
MMTDRVKKLREESLKAVPRISMERTRIVTDVYKKYEGTVSIPVLRALVLKELMERKELCI
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEE
YDGELIVGERGEAAAATPTYPELCCHTVEDFDIMDKREKISFKTTDEDKEIQQELIIPFW
ECCEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHCCHHHCCCCCCCCHHHHHHHHCCCHH
EKRSMRHKILEKMTPEWKACYEAGIFTEFMEQRGPGHTAGGDKYYKMGFLDIKEQIKEAI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHH
SKLDYLNDDEALDKKEQLDAMDIACDAIMIYGKRYSEYAAKLAQKEADPVRKKELMEISE
HHHCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VCSWVPAHAPRTFREAIQMYWFVHLCVISELNPWDAFNPGRLDQHLYPFYKKQIEEGTLD
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
REQARELLQCFWVKFNNQPAPPKVGITLKESGTYTDFANINSGGMKADGSDGVNDVSYLV
HHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LEVIDEMKLLQPSSNVQISKKTPQRFLKKACEVIRKGWGQPSIFNADSVVQELVRAGKSI
HHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCC
EDARCGGTSGCVEAGAFGKEAYILTGYFNLPKILEITLLNGVDSQTGKQLGIKTGDISTL
CHHCCCCCCCHHHCCCCCCCEEEEEECCCCCHHEEEEEECCCCCCCCCCCCCCCCCCHHH
KTYEDLLDAFKKQLKYFVDIKVNGNRVIERLYATLMPAPFLSVVTDDCIAKGKDYNAGGA
HHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC
RYNTSYIQGVGIGTITDSLSAIKYQVFDEKNITMEELMEALRFNFEGHEDIYNLVKNKTP
EECCHHHCCCCCHHHHHHHHHHEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCC
KYGNDDDYADEIMKEVFDAYYNEVNGRPNGREGCYRIDMLPTTCHVYFGSVINATPDGRK
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCE
AHIPVSEGISPSKGADVNGPTGVIKSAAKMDHLRTGGTLLNQKFVPSVVQGEEGIDNMAN
EECCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCHHHCCCCCHHHHHH
LVRGYFTMDGHHIQFNIVSKETLLKAQQNPDEYKDLIVRVAGYSDYFNNLDKVLQNEIIE
HHHHHHEECCCEEEEEEECHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RTEQEFN
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]