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The map label for this gene is wecB [C]

Identifier: 148381051

GI number: 148381051

Start: 3288615

End: 3289673

Strand: Reverse

Name: wecB [C]

Synonym: CBO3100

Alternate gene names: 148381051

Gene position: 3289673-3288615 (Counterclockwise)

Preceding gene: 148381052

Following gene: 148381050

Centisome position: 84.63

GC content: 28.52

Gene sequence:

>1059_bases
ATGAAGATATTAACAGTAGTTGGTGCACGTCCACAATTTATAAAAGCAGCAGCAGTTTCAAATATAATAAGAAAAGAGCA
TACGGAAATATTAGTTCATACTGGACAACATTATGATGAAAATATGTCTAAAATATTTTTTGAAGAATTAAGAATACCTA
AGCCAGATTATAATTTAGAAATAGGTTCAGGTAATCATGGTGAACAGACAGGTAAAATGCTTATAGAATTAGAAAAAATA
TATTTAAAAGAAAAACCGGATTTAGTATTAGTGTATGGAGATACAAATTCTACGTTAGCAGGGGCTTTGTGTGCAAGTAA
ATTGTTAATACCTGTAGCCCATGTAGAAGCTGGTCTTAGAAGCTTTAATATGAATATGCCAGAAGAACAAAATAGAATTC
TTACAGATCATATATCAAAGTTATTATTTGTACCTACAGTTACAGCAGAAAAAAATCTTCATACTGAGGGAGTTAATAAT
GGTGTTCATAATGTAGGAGATGTAATGTTTGATGCTGTATTACATTTTAAGAAATTAGCAGAAGAAAAGGAAAGTATACT
AGATAAAATCTCTATAAAATCTGGAGAATATATTTTAACAACAATACATAGAGCAGAAAACACTAATGATATAAATAGAT
TGAAAAATATAATAGAGGCATTAAATGAAAGTGGAAAGTCAATAGTATTACCACTTCATCCAAGAACTAAAAAATATATA
GAAGATTATAATTTACAATTTAATGATAATATAAAATTAATAGAGCCAGTAGGATATCTTGATATGATTACTTTAGAAAT
GAATTCACAAAAGATAGTTACTGATAGTGGTGGAGTTCAAAAAGAAGCTTTCTTTATGGAAAAACCTTGTGTTACTATGA
GAGATGAAACAGAGTGGGTAGAGACCGTAGAAAATGGATGGAATGTAATAGTAGGTACAGATAAAGAAAAAATATTAAAT
GCTGTAGTGAATTTTAAGCCTGAAAAAGAACAACAAGAAATATTTGGAAATGGTCATGCAGCGGAAAAAATATTAGATAT
AATAAACAAAATGAAGTAA

Upstream 100 bases:

>100_bases
AGACAGGAAGAAAATTTGTGGAAGATAATTTTAATATAGAAGATAATTTTAATAATGTAGATATAATATATAAATCTATA
ATAGATGGAGGAGATAATTA

Downstream 100 bases:

>100_bases
TTATTTTGAGGTGATAATATGAAAATCCTATTTTTGACTCAATACTGTCCACCTGAAGTTGGAGCACCTCAAAATAGAAT
TTTTGAGTTTGCAAAGCAAC

Product: UDP-N-acetylglucosamine 2-epimerase

Products: UDP-N-acetyl-D-mannosamine

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MKILTVVGARPQFIKAAAVSNIIRKEHTEILVHTGQHYDENMSKIFFEELRIPKPDYNLEIGSGNHGEQTGKMLIELEKI
YLKEKPDLVLVYGDTNSTLAGALCASKLLIPVAHVEAGLRSFNMNMPEEQNRILTDHISKLLFVPTVTAEKNLHTEGVNN
GVHNVGDVMFDAVLHFKKLAEEKESILDKISIKSGEYILTTIHRAENTNDINRLKNIIEALNESGKSIVLPLHPRTKKYI
EDYNLQFNDNIKLIEPVGYLDMITLEMNSQKIVTDSGGVQKEAFFMEKPCVTMRDETEWVETVENGWNVIVGTDKEKILN
AVVNFKPEKEQQEIFGNGHAAEKILDIINKMK

Sequences:

>Translated_352_residues
MKILTVVGARPQFIKAAAVSNIIRKEHTEILVHTGQHYDENMSKIFFEELRIPKPDYNLEIGSGNHGEQTGKMLIELEKI
YLKEKPDLVLVYGDTNSTLAGALCASKLLIPVAHVEAGLRSFNMNMPEEQNRILTDHISKLLFVPTVTAEKNLHTEGVNN
GVHNVGDVMFDAVLHFKKLAEEKESILDKISIKSGEYILTTIHRAENTNDINRLKNIIEALNESGKSIVLPLHPRTKKYI
EDYNLQFNDNIKLIEPVGYLDMITLEMNSQKIVTDSGGVQKEAFFMEKPCVTMRDETEWVETVENGWNVIVGTDKEKILN
AVVNFKPEKEQQEIFGNGHAAEKILDIINKMK
>Mature_352_residues
MKILTVVGARPQFIKAAAVSNIIRKEHTEILVHTGQHYDENMSKIFFEELRIPKPDYNLEIGSGNHGEQTGKMLIELEKI
YLKEKPDLVLVYGDTNSTLAGALCASKLLIPVAHVEAGLRSFNMNMPEEQNRILTDHISKLLFVPTVTAEKNLHTEGVNN
GVHNVGDVMFDAVLHFKKLAEEKESILDKISIKSGEYILTTIHRAENTNDINRLKNIIEALNESGKSIVLPLHPRTKKYI
EDYNLQFNDNIKLIEPVGYLDMITLEMNSQKIVTDSGGVQKEAFFMEKPCVTMRDETEWVETVENGWNVIVGTDKEKILN
AVVNFKPEKEQQEIFGNGHAAEKILDIINKMK

Specific function: Catalyzes The Reversible Epimerization At C-2 Of UDP-N- Acetylglucosamine (UDP-Glcnac) And Thereby Provides Bacteria With UDP-N-Acetylmannosamine (UDP-Mannac), The Activated Donor Of Mannac Residues. Also Involved In Bacteriophage N4 Adsorption. [C]

COG id: COG0381

COG function: function code M; UDP-N-acetylglucosamine 2-epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-N-acetylglucosamine 2-epimerase family [H]

Homologues:

Organism=Escherichia coli, GI48994967, Length=378, Percent_Identity=34.3915343915344, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003331 [H]

Pfam domain/function: PF02350 Epimerase_2 [H]

EC number: 5.1.3.14

Molecular weight: Translated: 39805; Mature: 39805

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILTVVGARPQFIKAAAVSNIIRKEHTEILVHTGQHYDENMSKIFFEELRIPKPDYNLE
CEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEE
IGSGNHGEQTGKMLIELEKIYLKEKPDLVLVYGDTNSTLAGALCASKLLIPVAHVEAGLR
ECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
SFNMNMPEEQNRILTDHISKLLFVPTVTAEKNLHTEGVNNGVHNVGDVMFDAVLHFKKLA
HCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
EEKESILDKISIKSGEYILTTIHRAENTNDINRLKNIIEALNESGKSIVLPLHPRTKKYI
HHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHH
EDYNLQFNDNIKLIEPVGYLDMITLEMNSQKIVTDSGGVQKEAFFMEKPCVTMRDETEWV
HHCCEEECCCEEEECCCCCEEEEEEEECCCEEEECCCCCCHHHHHHCCCCCCCCCCHHHH
ETVENGWNVIVGTDKEKILNAVVNFKPEKEQQEIFGNGHAAEKILDIINKMK
HHHHCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILTVVGARPQFIKAAAVSNIIRKEHTEILVHTGQHYDENMSKIFFEELRIPKPDYNLE
CEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEE
IGSGNHGEQTGKMLIELEKIYLKEKPDLVLVYGDTNSTLAGALCASKLLIPVAHVEAGLR
ECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
SFNMNMPEEQNRILTDHISKLLFVPTVTAEKNLHTEGVNNGVHNVGDVMFDAVLHFKKLA
HCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
EEKESILDKISIKSGEYILTTIHRAENTNDINRLKNIIEALNESGKSIVLPLHPRTKKYI
HHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHH
EDYNLQFNDNIKLIEPVGYLDMITLEMNSQKIVTDSGGVQKEAFFMEKPCVTMRDETEWV
HHCCEEECCCEEEECCCCCEEEEEEEECCCEEEECCCCCCHHHHHHCCCCCCCCCCHHHH
ETVENGWNVIVGTDKEKILNAVVNFKPEKEQQEIFGNGHAAEKILDIINKMK
HHHHCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: UDP-N-acetylglucosamine [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.63 {UDP-N-acetylglucosamine}} 1 {UDP-N-acetylmannosamine}} 0.5 {UDP-N-acetylmannosamine}} [C]

Substrates: UDP-N-acetyl-D-glucosamine

Specific reaction: UDP-N-acetyl-D-glucosamine = UDP-N-acetyl-D-mannosamine

General reaction: Epimerization [C]

Inhibitor: PCMB [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA