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The map label for this gene is wcaI [H]

Identifier: 148381050

GI number: 148381050

Start: 3287370

End: 3288596

Strand: Reverse

Name: wcaI [H]

Synonym: CBO3099

Alternate gene names: 148381050

Gene position: 3288596-3287370 (Counterclockwise)

Preceding gene: 148381051

Following gene: 148381049

Centisome position: 84.61

GC content: 29.99

Gene sequence:

>1227_bases
ATGAAAATCCTATTTTTGACTCAATACTGTCCACCTGAAGTTGGAGCACCTCAAAATAGAATTTTTGAGTTTGCAAAGCA
ACTAAAAAAATTCGGTCATGAGGTTACAATATTAACGGCTATGCCAAATTACCCTAAAGGTGAAATATTTGATGGTTATA
AGGGAAAGAAAATAGTAAAAGAGGAACTGGATGGAATAAATATCGTTCGTACAAGTATATATGCAACGAAGGATAAATCC
TTTGTAAAAAGACTTAGAAATTATCTTTCGTTTACTTTTTCTTCAGTATTTACAGGCTCTAAATATATTGATAATCAGGA
TGCTATAATAACAGAATCTCCACCGCTATTTTTAGGGTGGTCAGGATATATTTTATCTAAAAGGAAAAAAGCAAAATTTA
TTTTTAATGTATCTGATTTATGGCCAGAATCTGCTGTAAAATTAGATGTATTACATAATAAATTTCTTATAAAGGCATCT
ACATGGCTTGAAGAGTTTTGTTATAAAAAAGCAGCAGCAGTTACTGGGCAAACAAAGGGTATAGTTGATAATATTGTAAG
TAGAGGTTTTGATAAAAATAAAGTTCATTTAATAACTAATGGAGTGGATACAGAATTTTTTAAAAAAGAAAATAGGGATG
AAAGCTTAAGAGAAGAATGGGGATTAAAGGATAAATTTGCAGTATGTTATGCGGGTATACATGGCCTAGCTCAAGGATTA
GAAGTTGTTATAAATGCAGCGGAATTATTAAAAGAAGAAAGAGATATACAATTTGTATTTATAGGAGATGGACCAGAAAA
ATCTGAGCTTATGACTATGGTAAAAGAAAAAAAATTAACTAATGTATCTTTTCAGCCAATGCAGCTTAAGCCTAATATGC
CTAGAATAATAGCATCTATGGATGCTACCGTAGTTCCTTTAAAAAAATTAGATTTATTTAAGGGTGCACTACCATCAAAA
ATGTTTGAAGCATTGGCATCTGAATTGCCTATAGTATTAGCTGTAGAAGGGGAAGCAGAAAAGCTTATAAATGAAGCTAA
TGCTGGAATAACTGTAGAACCAGAGAATGCAAAAGAAGTTTCACAAGCAGTTTTAAAGTTATATAAAAATAAGGATATTA
AAGAGAAATTGGGACAAAATGGTAGGAATTATGTTATAAAAAATTATTCTAGAGAAAGTATAACTAGAAAGTTAGAAAAT
ATATTGTTAAAATTAAAGAATAGTTAA

Upstream 100 bases:

>100_bases
GCCTGAAAAAGAACAACAAGAAATATTTGGAAATGGTCATGCAGCGGAAAAAATATTAGATATAATAAACAAAATGAAGT
AATTATTTTGAGGTGATAAT

Downstream 100 bases:

>100_bases
TACCTCTATATAAATAGATTGAATATAATTGTTCACTATTGTTATTTCTATAATCTTTAGTTTAAGGGGACATACTTATG
GAAAGTTTAAGTAATATTAT

Product: glycosyl transferase, group 1 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MKILFLTQYCPPEVGAPQNRIFEFAKQLKKFGHEVTILTAMPNYPKGEIFDGYKGKKIVKEELDGINIVRTSIYATKDKS
FVKRLRNYLSFTFSSVFTGSKYIDNQDAIITESPPLFLGWSGYILSKRKKAKFIFNVSDLWPESAVKLDVLHNKFLIKAS
TWLEEFCYKKAAAVTGQTKGIVDNIVSRGFDKNKVHLITNGVDTEFFKKENRDESLREEWGLKDKFAVCYAGIHGLAQGL
EVVINAAELLKEERDIQFVFIGDGPEKSELMTMVKEKKLTNVSFQPMQLKPNMPRIIASMDATVVPLKKLDLFKGALPSK
MFEALASELPIVLAVEGEAEKLINEANAGITVEPENAKEVSQAVLKLYKNKDIKEKLGQNGRNYVIKNYSRESITRKLEN
ILLKLKNS

Sequences:

>Translated_408_residues
MKILFLTQYCPPEVGAPQNRIFEFAKQLKKFGHEVTILTAMPNYPKGEIFDGYKGKKIVKEELDGINIVRTSIYATKDKS
FVKRLRNYLSFTFSSVFTGSKYIDNQDAIITESPPLFLGWSGYILSKRKKAKFIFNVSDLWPESAVKLDVLHNKFLIKAS
TWLEEFCYKKAAAVTGQTKGIVDNIVSRGFDKNKVHLITNGVDTEFFKKENRDESLREEWGLKDKFAVCYAGIHGLAQGL
EVVINAAELLKEERDIQFVFIGDGPEKSELMTMVKEKKLTNVSFQPMQLKPNMPRIIASMDATVVPLKKLDLFKGALPSK
MFEALASELPIVLAVEGEAEKLINEANAGITVEPENAKEVSQAVLKLYKNKDIKEKLGQNGRNYVIKNYSRESITRKLEN
ILLKLKNS
>Mature_408_residues
MKILFLTQYCPPEVGAPQNRIFEFAKQLKKFGHEVTILTAMPNYPKGEIFDGYKGKKIVKEELDGINIVRTSIYATKDKS
FVKRLRNYLSFTFSSVFTGSKYIDNQDAIITESPPLFLGWSGYILSKRKKAKFIFNVSDLWPESAVKLDVLHNKFLIKAS
TWLEEFCYKKAAAVTGQTKGIVDNIVSRGFDKNKVHLITNGVDTEFFKKENRDESLREEWGLKDKFAVCYAGIHGLAQGL
EVVINAAELLKEERDIQFVFIGDGPEKSELMTMVKEKKLTNVSFQPMQLKPNMPRIIASMDATVVPLKKLDLFKGALPSK
MFEALASELPIVLAVEGEAEKLINEANAGITVEPENAKEVSQAVLKLYKNKDIKEKLGQNGRNYVIKNYSRESITRKLEN
ILLKLKNS

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788363, Length=410, Percent_Identity=25.8536585365854, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 46195; Mature: 46195

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILFLTQYCPPEVGAPQNRIFEFAKQLKKFGHEVTILTAMPNYPKGEIFDGYKGKKIVK
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHH
EELDGINIVRTSIYATKDKSFVKRLRNYLSFTFSSVFTGSKYIDNQDAIITESPPLFLGW
HHCCCCEEEEHHEEECCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEECCCCEEEEC
SGYILSKRKKAKFIFNVSDLWPESAVKLDVLHNKFLIKASTWLEEFCYKKAAAVTGQTKG
CCCEECCCCCCEEEEEHHHHCCCHHEEEEEECCEEEEEHHHHHHHHHHHHHHHCCCCCHH
IVDNIVSRGFDKNKVHLITNGVDTEFFKKENRDESLREEWGLKDKFAVCYAGIHGLAQGL
HHHHHHHCCCCCCEEEEEECCCCHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHH
EVVINAAELLKEERDIQFVFIGDGPEKSELMTMVKEKKLTNVSFQPMQLKPNMPRIIASM
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHC
DATVVPLKKLDLFKGALPSKMFEALASELPIVLAVEGEAEKLINEANAGITVEPENAKEV
CCCEECHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCEEECCCCHHHH
SQAVLKLYKNKDIKEKLGQNGRNYVIKNYSRESITRKLENILLKLKNS
HHHHHHHHCCCCHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKILFLTQYCPPEVGAPQNRIFEFAKQLKKFGHEVTILTAMPNYPKGEIFDGYKGKKIVK
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHH
EELDGINIVRTSIYATKDKSFVKRLRNYLSFTFSSVFTGSKYIDNQDAIITESPPLFLGW
HHCCCCEEEEHHEEECCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEECCCCEEEEC
SGYILSKRKKAKFIFNVSDLWPESAVKLDVLHNKFLIKASTWLEEFCYKKAAAVTGQTKG
CCCEECCCCCCEEEEEHHHHCCCHHEEEEEECCEEEEEHHHHHHHHHHHHHHHCCCCCHH
IVDNIVSRGFDKNKVHLITNGVDTEFFKKENRDESLREEWGLKDKFAVCYAGIHGLAQGL
HHHHHHHCCCCCCEEEEEECCCCHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHH
EVVINAAELLKEERDIQFVFIGDGPEKSELMTMVKEKKLTNVSFQPMQLKPNMPRIIASM
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHC
DATVVPLKKLDLFKGALPSKMFEALASELPIVLAVEGEAEKLINEANAGITVEPENAKEV
CCCEECHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCEEECCCCHHHH
SQAVLKLYKNKDIKEKLGQNGRNYVIKNYSRESITRKLENILLKLKNS
HHHHHHHHCCCCHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503 [H]