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The map label for this gene is hisS

Identifier: 148381006

GI number: 148381006

Start: 3242263

End: 3243510

Strand: Reverse

Name: hisS

Synonym: CBO3055

Alternate gene names: 148381006

Gene position: 3243510-3242263 (Counterclockwise)

Preceding gene: 148381007

Following gene: 148381005

Centisome position: 83.45

GC content: 29.73

Gene sequence:

>1248_bases
ATGTCATTGCAAGCGCCAAAAGGTACAAAAGATTTATTGCCTACAGAATCATATAAGTGGCAATATTTAGAGAATAAATT
TAGAAATATAGCAGCAGATTTTGGCTGTAGAGAAATAAGAACTCCAGTTTTTGAATATACAGAATTATTTCAAAGAGGAG
TAGGGGAAACTACAGATGTAGTTCAAAAGGAAATGTATACTTTTGAAGATAAAGCTGGTAGAAGTATAACTTTAAAACCA
GAAGGAACATCACCAGCAGTAAGAGCCTTCGTTGAGGGAAGATTATTCAATGAAACTCAACCTACTAAGATGTATTATTT
TACACCTGTTATGAGATATGAGAATGTTCAAAAGGGAAGACTTAGACAACATCATCAATTTGGAATAGAAATTTTTGGAG
CAAAAGATGCATCTGTGGATGCAGAAGTTATAAGTATACCTGTTGGAATATATAAAGAATTAGGTGTAGAGGGTGTAGAA
TTAAATATAAATAGCATAGGTTGCCCTAAATGTAGAAAAACTTATAATGAAGCACTAAAAAAATATTTATCTAAAAATTA
TGATAAATTATGCTCAACTTGTAAAACTAGATTTGATAAAAACCCACTTAGAATATTAGATTGCAAAGTTGATACTTGTA
AAGAAATAGTAAAGGATGCACCAATTATATTAGATTATATATGTGATGAATGTAAGGACCATTTTGAAGCACTAAAAAGT
TATTTAGATGTATTGGATATAAAATATAAAGTAGATCCATTTATAGTTAGAGGATTAGATTATTATAGTAAAACTGTGTT
TGAATTTATAATAGATGATATAACTATATGTGCTGGGGGAAGATATGATTATCTTATAGAAGAAATAGGTGGGCCTTCTA
TGCCAGCAGTAGGTTTTGGTATGGGAATAGAAAGATTACTTTTAACATTGCAAGAAAAAGCAATAGAAATTCCTGAAGAA
GCATATGTAGATTTATATTTAGGAAATATGGGAGATAAAGCTAAATTAGAGGTTTTAAAATTAGCTAAAGAATTAAGGGA
TAGACATATAAAGTGTGAAATAGACCATATGGGCAAAAGTGTTAAAGCACAAATGAAGTATGCAAATAGGATAGGTGCTA
AATATTCTATGGTATTAGGTGAAGAAGAATTAAATACTGGAAAGGTATCCCTTAAAAGAATGGAAGATGGGCAGCAAATA
GAAGTCGATATAAAAGAGATAGATACATTAATTAAAGTATTTAAATAG

Upstream 100 bases:

>100_bases
TAAGGATGTAATAGAATATACAAAAAGAGTGGAAGAAATGGTACATAGAAAGAAAAAAATATTAGATACTTTATATTTAT
AATGGTTAGGAGGAATATAA

Downstream 100 bases:

>100_bases
ACAAAAATAAAATAACAAATCTATATTTGGAGGAATAAAAATGGGAGAAGCGCTAAGAGGACTTAAGAGAACAATAATGT
GTGGCGAGTCTAGAGAAAAT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MSLQAPKGTKDLLPTESYKWQYLENKFRNIAADFGCREIRTPVFEYTELFQRGVGETTDVVQKEMYTFEDKAGRSITLKP
EGTSPAVRAFVEGRLFNETQPTKMYYFTPVMRYENVQKGRLRQHHQFGIEIFGAKDASVDAEVISIPVGIYKELGVEGVE
LNINSIGCPKCRKTYNEALKKYLSKNYDKLCSTCKTRFDKNPLRILDCKVDTCKEIVKDAPIILDYICDECKDHFEALKS
YLDVLDIKYKVDPFIVRGLDYYSKTVFEFIIDDITICAGGRYDYLIEEIGGPSMPAVGFGMGIERLLLTLQEKAIEIPEE
AYVDLYLGNMGDKAKLEVLKLAKELRDRHIKCEIDHMGKSVKAQMKYANRIGAKYSMVLGEEELNTGKVSLKRMEDGQQI
EVDIKEIDTLIKVFK

Sequences:

>Translated_415_residues
MSLQAPKGTKDLLPTESYKWQYLENKFRNIAADFGCREIRTPVFEYTELFQRGVGETTDVVQKEMYTFEDKAGRSITLKP
EGTSPAVRAFVEGRLFNETQPTKMYYFTPVMRYENVQKGRLRQHHQFGIEIFGAKDASVDAEVISIPVGIYKELGVEGVE
LNINSIGCPKCRKTYNEALKKYLSKNYDKLCSTCKTRFDKNPLRILDCKVDTCKEIVKDAPIILDYICDECKDHFEALKS
YLDVLDIKYKVDPFIVRGLDYYSKTVFEFIIDDITICAGGRYDYLIEEIGGPSMPAVGFGMGIERLLLTLQEKAIEIPEE
AYVDLYLGNMGDKAKLEVLKLAKELRDRHIKCEIDHMGKSVKAQMKYANRIGAKYSMVLGEEELNTGKVSLKRMEDGQQI
EVDIKEIDTLIKVFK
>Mature_414_residues
SLQAPKGTKDLLPTESYKWQYLENKFRNIAADFGCREIRTPVFEYTELFQRGVGETTDVVQKEMYTFEDKAGRSITLKPE
GTSPAVRAFVEGRLFNETQPTKMYYFTPVMRYENVQKGRLRQHHQFGIEIFGAKDASVDAEVISIPVGIYKELGVEGVEL
NINSIGCPKCRKTYNEALKKYLSKNYDKLCSTCKTRFDKNPLRILDCKVDTCKEIVKDAPIILDYICDECKDHFEALKSY
LDVLDIKYKVDPFIVRGLDYYSKTVFEFIIDDITICAGGRYDYLIEEIGGPSMPAVGFGMGIERLLLTLQEKAIEIPEEA
YVDLYLGNMGDKAKLEVLKLAKELRDRHIKCEIDHMGKSVKAQMKYANRIGAKYSMVLGEEELNTGKVSLKRMEDGQQIE
VDIKEIDTLIKVFK

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=456, Percent_Identity=24.3421052631579, Blast_Score=96, Evalue=9e-20,
Organism=Escherichia coli, GI1788861, Length=409, Percent_Identity=42.5427872860636, Blast_Score=343, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI71993693, Length=460, Percent_Identity=23.2608695652174, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71993686, Length=460, Percent_Identity=23.2608695652174, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6325290, Length=478, Percent_Identity=24.2677824267782, Blast_Score=100, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_CLOB1 (A7FY04)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385377.1
- ProteinModelPortal:   A7FY04
- SMR:   A7FY04
- STRING:   A7FY04
- GeneID:   5394988
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3084
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- BioCyc:   CBOT441770:CLB_3084-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47507; Mature: 47376

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQAPKGTKDLLPTESYKWQYLENKFRNIAADFGCREIRTPVFEYTELFQRGVGETTDV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHCCCCHHHH
VQKEMYTFEDKAGRSITLKPEGTSPAVRAFVEGRLFNETQPTKMYYFTPVMRYENVQKGR
HHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCEEEEEEHHHHHCCCHHHH
LRQHHQFGIEIFGAKDASVDAEVISIPVGIYKELGVEGVELNINSIGCPKCRKTYNEALK
HHHHHHCCEEEEECCCCCCCCCCEEECHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHH
KYLSKNYDKLCSTCKTRFDKNPLRILDCKVDTCKEIVKDAPIILDYICDECKDHFEALKS
HHHHCCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
YLDVLDIKYKVDPFIVRGLDYYSKTVFEFIIDDITICAGGRYDYLIEEIGGPSMPAVGFG
HHHHHHCEEECCHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHCCCCCCCCHHHH
MGIERLLLTLQEKAIEIPEEAYVDLYLGNMGDKAKLEVLKLAKELRDRHIKCEIDHMGKS
HHHHHHHHHHHHHHHCCCCHHEEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEHHHCCHH
VKAQMKYANRIGAKYSMVLGEEELNTGKVSLKRMEDGQQIEVDIKEIDTLIKVFK
HHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHCC
>Mature Secondary Structure 
SLQAPKGTKDLLPTESYKWQYLENKFRNIAADFGCREIRTPVFEYTELFQRGVGETTDV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHCCCCHHHH
VQKEMYTFEDKAGRSITLKPEGTSPAVRAFVEGRLFNETQPTKMYYFTPVMRYENVQKGR
HHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCEEEEEEHHHHHCCCHHHH
LRQHHQFGIEIFGAKDASVDAEVISIPVGIYKELGVEGVELNINSIGCPKCRKTYNEALK
HHHHHHCCEEEEECCCCCCCCCCEEECHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHH
KYLSKNYDKLCSTCKTRFDKNPLRILDCKVDTCKEIVKDAPIILDYICDECKDHFEALKS
HHHHCCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
YLDVLDIKYKVDPFIVRGLDYYSKTVFEFIIDDITICAGGRYDYLIEEIGGPSMPAVGFG
HHHHHHCEEECCHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHCCCCCCCCHHHH
MGIERLLLTLQEKAIEIPEEAYVDLYLGNMGDKAKLEVLKLAKELRDRHIKCEIDHMGKS
HHHHHHHHHHHHHHHCCCCHHEEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEHHHCCHH
VKAQMKYANRIGAKYSMVLGEEELNTGKVSLKRMEDGQQIEVDIKEIDTLIKVFK
HHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA