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The map label for this gene is aspS

Identifier: 148381005

GI number: 148381005

Start: 3240441

End: 3242222

Strand: Reverse

Name: aspS

Synonym: CBO3054

Alternate gene names: 148381005

Gene position: 3242222-3240441 (Counterclockwise)

Preceding gene: 148381006

Following gene: 148381003

Centisome position: 83.41

GC content: 30.7

Gene sequence:

>1782_bases
ATGGGAGAAGCGCTAAGAGGACTTAAGAGAACAATAATGTGTGGCGAGTCTAGAGAAAATAATATAGGACAAAAAGTCAC
AGTTATGGGATGGGTTCAAAGAAAAAGAAATTTAGGAGGACTAATCTTTGTAGATTTAAGAGATAGAACAGGAATTATGC
AAATAGTTTTTGGTGAAGAAATAAATAAAGAAGCCTTCGAAAAATCAGATAATGTAAAATCAGAATATTGTATCGCCGTA
ACTGGTGAAATAGTTAAAAGACAGTCACCAAACAATGATATGGAAACTGGAGCTGTGGAATTAAAGGGAGAAGATATAAA
AATATTATCTGAATCAGAAACACCTCCTATATATATAAAAGAAGGGTTAGATGCATCTGAAAATATAAGATTAAAATATA
GATATCTTGACTTAAGAAGACCAGATATGCAAAAGATATTTATGATAAGACACAAGACATGTAAAGTTGTTAGAGATTTC
TTAGATGAAAATGGATTTTTAGAAATGGAAACACCAATACTTACAAAAAGTACCCCAGAAGGAGCTCGTGACTATTTAGT
TCCAAGTAGAAATTACAAAGGAATGTTTTATGCATTACCACAATCACCACAAATATTTAAACAGCTTTTAATGGTCTCAG
GTTATGATAAATATTTTCAAATAACAAAATGTTTTAGAGATGAAGATTTAAGAGCTAATAGACAACCGGAATTTACACAA
ATAGATATGGAATTATCTTTTGTAGAAGAAGATGATGTAATTGAATTAAACGAAAGATTATTAGCTAAGGTATTTAAAGA
AGTTGGGGGAATAGATGTAAAACTTCCGATAGAAAGAATGCCATATAAAATAGCTATGGAAAAATACGGTTCAGATAAAC
CAGATTTAAGATTTGGAATGGAAATAAATGATTTAACAGAAGCTGTTAAAAATTCAGAATTTAAAGTATTCAAAGGTGCT
ATAGAAGCAGGTGGCTCTGTAAGAGCAATAAAAGCAGAAAATTGTGCTACTATGGGAAGAAAACAAATAGATAAGCTACA
AGATTTTGTTAAAACATATAAAGCAAAAGGATTAGCTTGGATAGCTTATAAAGAGGATGAAATAAAATCTCCAATAGCTA
AATTTTTAACAGAAGAAGAAATGAAAGCTATATTAGAAAAAATGGATGCTAAAGCAGGAGATTTAATATTAATAGTAGCA
GACAAAAACAATGTAGTATTTGAAAGCTTAGGGGCATTAAGACTACATATAGCAAAAGAATTAGATATTATAAATAAAAA
TGAGTTTAGATTTGTGTGGATAACTGAATTCCCACTATTAGCTTATAATGAAGAAGAAGGCAGATATCAAGCGGAGCATC
ATCCATTCACAGCTATAATGGATGAGGATATAGAACTATTAGGTACAGAGCCAGGAAAAGTAAGAGCGAAAGCTTATGAT
ATAGTATTAAATGGAGAAGAGCTAGGCGGAGGAAGTATTAGAATACATGATAGTAAATTTCAAGAAAAAATGTTTAGTGT
ATTAGGCTTTACTAAAGAAAAAGCTTGGGAAAGATTTGGGTTCTTACTTGAAGCATTTAAATTTGGACCACCACCACATG
GTGGGTTAGCTTATGGTCTTGATAGAATGATAATGTTTTTAGCAGGAACAGAAAATATTAAAGACGTAATAACATTCCCT
AAGAATCAAAATGCCTTCTGCCCATTAACTGAAGCACCAAATGTAGTTGATGAAAATCAATTAGAAGAATTAGGAATAAA
AAAAATAGAAAAAGAAGATTAA

Upstream 100 bases:

>100_bases
GGGCAGCAAATAGAAGTCGATATAAAAGAGATAGATACATTAATTAAAGTATTTAAATAGACAAAAATAAAATAACAAAT
CTATATTTGGAGGAATAAAA

Downstream 100 bases:

>100_bases
TAAAATAAGGAAAGTGCTATGCGCTTTCCTTATTTTATCTACTAAGTATTCGTGTCATTTTAAGAGTGTAGCTTTGTTTT
TTACCCAATTTTTTATCCAT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 593; Mature: 592

Protein sequence:

>593_residues
MGEALRGLKRTIMCGESRENNIGQKVTVMGWVQRKRNLGGLIFVDLRDRTGIMQIVFGEEINKEAFEKSDNVKSEYCIAV
TGEIVKRQSPNNDMETGAVELKGEDIKILSESETPPIYIKEGLDASENIRLKYRYLDLRRPDMQKIFMIRHKTCKVVRDF
LDENGFLEMETPILTKSTPEGARDYLVPSRNYKGMFYALPQSPQIFKQLLMVSGYDKYFQITKCFRDEDLRANRQPEFTQ
IDMELSFVEEDDVIELNERLLAKVFKEVGGIDVKLPIERMPYKIAMEKYGSDKPDLRFGMEINDLTEAVKNSEFKVFKGA
IEAGGSVRAIKAENCATMGRKQIDKLQDFVKTYKAKGLAWIAYKEDEIKSPIAKFLTEEEMKAILEKMDAKAGDLILIVA
DKNNVVFESLGALRLHIAKELDIINKNEFRFVWITEFPLLAYNEEEGRYQAEHHPFTAIMDEDIELLGTEPGKVRAKAYD
IVLNGEELGGGSIRIHDSKFQEKMFSVLGFTKEKAWERFGFLLEAFKFGPPPHGGLAYGLDRMIMFLAGTENIKDVITFP
KNQNAFCPLTEAPNVVDENQLEELGIKKIEKED

Sequences:

>Translated_593_residues
MGEALRGLKRTIMCGESRENNIGQKVTVMGWVQRKRNLGGLIFVDLRDRTGIMQIVFGEEINKEAFEKSDNVKSEYCIAV
TGEIVKRQSPNNDMETGAVELKGEDIKILSESETPPIYIKEGLDASENIRLKYRYLDLRRPDMQKIFMIRHKTCKVVRDF
LDENGFLEMETPILTKSTPEGARDYLVPSRNYKGMFYALPQSPQIFKQLLMVSGYDKYFQITKCFRDEDLRANRQPEFTQ
IDMELSFVEEDDVIELNERLLAKVFKEVGGIDVKLPIERMPYKIAMEKYGSDKPDLRFGMEINDLTEAVKNSEFKVFKGA
IEAGGSVRAIKAENCATMGRKQIDKLQDFVKTYKAKGLAWIAYKEDEIKSPIAKFLTEEEMKAILEKMDAKAGDLILIVA
DKNNVVFESLGALRLHIAKELDIINKNEFRFVWITEFPLLAYNEEEGRYQAEHHPFTAIMDEDIELLGTEPGKVRAKAYD
IVLNGEELGGGSIRIHDSKFQEKMFSVLGFTKEKAWERFGFLLEAFKFGPPPHGGLAYGLDRMIMFLAGTENIKDVITFP
KNQNAFCPLTEAPNVVDENQLEELGIKKIEKED
>Mature_592_residues
GEALRGLKRTIMCGESRENNIGQKVTVMGWVQRKRNLGGLIFVDLRDRTGIMQIVFGEEINKEAFEKSDNVKSEYCIAVT
GEIVKRQSPNNDMETGAVELKGEDIKILSESETPPIYIKEGLDASENIRLKYRYLDLRRPDMQKIFMIRHKTCKVVRDFL
DENGFLEMETPILTKSTPEGARDYLVPSRNYKGMFYALPQSPQIFKQLLMVSGYDKYFQITKCFRDEDLRANRQPEFTQI
DMELSFVEEDDVIELNERLLAKVFKEVGGIDVKLPIERMPYKIAMEKYGSDKPDLRFGMEINDLTEAVKNSEFKVFKGAI
EAGGSVRAIKAENCATMGRKQIDKLQDFVKTYKAKGLAWIAYKEDEIKSPIAKFLTEEEMKAILEKMDAKAGDLILIVAD
KNNVVFESLGALRLHIAKELDIINKNEFRFVWITEFPLLAYNEEEGRYQAEHHPFTAIMDEDIELLGTEPGKVRAKAYDI
VLNGEELGGGSIRIHDSKFQEKMFSVLGFTKEKAWERFGFLLEAFKFGPPPHGGLAYGLDRMIMFLAGTENIKDVITFPK
NQNAFCPLTEAPNVVDENQLEELGIKKIEKED

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=603, Percent_Identity=37.6451077943615, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI5031815, Length=251, Percent_Identity=29.0836653386454, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI194272210, Length=251, Percent_Identity=29.0836653386454, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI45439306, Length=283, Percent_Identity=25.7950530035336, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI4758762, Length=282, Percent_Identity=27.3049645390071, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI226491502, Length=261, Percent_Identity=24.904214559387, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788173, Length=591, Percent_Identity=47.3773265651438, Blast_Score=559, Evalue=1e-160,
Organism=Escherichia coli, GI1790571, Length=291, Percent_Identity=26.8041237113402, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1789256, Length=281, Percent_Identity=25.2669039145907, Blast_Score=96, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI32566633, Length=595, Percent_Identity=34.6218487394958, Blast_Score=313, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI71994340, Length=302, Percent_Identity=25.4966887417219, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17535925, Length=302, Percent_Identity=25.4966887417219, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17535927, Length=302, Percent_Identity=25.4966887417219, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17551876, Length=270, Percent_Identity=28.1481481481481, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71984122, Length=295, Percent_Identity=25.4237288135593, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=626, Percent_Identity=35.7827476038339, Blast_Score=298, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6321807, Length=254, Percent_Identity=29.1338582677165, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323011, Length=282, Percent_Identity=26.9503546099291, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320242, Length=255, Percent_Identity=25.0980392156863, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324256, Length=271, Percent_Identity=22.5092250922509, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24584738, Length=598, Percent_Identity=35.4515050167224, Blast_Score=345, Evalue=7e-95,
Organism=Drosophila melanogaster, GI17136276, Length=239, Percent_Identity=30.5439330543933, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24640849, Length=264, Percent_Identity=28.030303030303, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24640851, Length=264, Percent_Identity=28.030303030303, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19921528, Length=232, Percent_Identity=25.4310344827586, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_CLOB1 (A7FY03)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385376.1
- ProteinModelPortal:   A7FY03
- SMR:   A7FY03
- STRING:   A7FY03
- GeneID:   5394649
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3083
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   CBOT441770:CLB_3083-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 67809; Mature: 67678

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEALRGLKRTIMCGESRENNIGQKVTVMGWVQRKRNLGGLIFVDLRDRTGIMQIVFGEE
CCHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHCCCCCEEEEEECCCCCEEEEEECCC
INKEAFEKSDNVKSEYCIAVTGEIVKRQSPNNDMETGAVELKGEDIKILSESETPPIYIK
CCHHHHHCCCCCCCCEEEEEEHHHHHCCCCCCCCCCCEEEECCCCEEEEECCCCCCEEEE
EGLDASENIRLKYRYLDLRRPDMQKIFMIRHKTCKVVRDFLDENGFLEMETPILTKSTPE
CCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCC
GARDYLVPSRNYKGMFYALPQSPQIFKQLLMVSGYDKYFQITKCFRDEDLRANRQPEFTQ
CCHHCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEE
IDMELSFVEEDDVIELNERLLAKVFKEVGGIDVKLPIERMPYKIAMEKYGSDKPDLRFGM
EEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECHHHCCCEEEHHHCCCCCCCEEECC
EINDLTEAVKNSEFKVFKGAIEAGGSVRAIKAENCATMGRKQIDKLQDFVKTYKAKGLAW
CHHHHHHHHHCCCEEEEEHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEE
IAYKEDEIKSPIAKFLTEEEMKAILEKMDAKAGDLILIVADKNNVVFESLGALRLHIAKE
EEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEHHHHCHHHHHHHHH
LDIINKNEFRFVWITEFPLLAYNEEEGRYQAEHHPFTAIMDEDIELLGTEPGKVRAKAYD
HCEECCCCEEEEEEECCCEEEEECCCCCEECCCCCEEEEECCCHHEECCCCCCEEEEEEE
IVLNGEELGGGSIRIHDSKFQEKMFSVLGFTKEKAWERFGFLLEAFKFGPPPHGGLAYGL
EEEECCCCCCCEEEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
DRMIMFLAGTENIKDVITFPKNQNAFCPLTEAPNVVDENQLEELGIKKIEKED
HHHHHHHCCCCCCHHHEECCCCCCCEECCCCCCCCCCCHHHHHHCHHHHCCCC
>Mature Secondary Structure 
GEALRGLKRTIMCGESRENNIGQKVTVMGWVQRKRNLGGLIFVDLRDRTGIMQIVFGEE
CHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHCCCCCEEEEEECCCCCEEEEEECCC
INKEAFEKSDNVKSEYCIAVTGEIVKRQSPNNDMETGAVELKGEDIKILSESETPPIYIK
CCHHHHHCCCCCCCCEEEEEEHHHHHCCCCCCCCCCCEEEECCCCEEEEECCCCCCEEEE
EGLDASENIRLKYRYLDLRRPDMQKIFMIRHKTCKVVRDFLDENGFLEMETPILTKSTPE
CCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCC
GARDYLVPSRNYKGMFYALPQSPQIFKQLLMVSGYDKYFQITKCFRDEDLRANRQPEFTQ
CCHHCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEE
IDMELSFVEEDDVIELNERLLAKVFKEVGGIDVKLPIERMPYKIAMEKYGSDKPDLRFGM
EEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECHHHCCCEEEHHHCCCCCCCEEECC
EINDLTEAVKNSEFKVFKGAIEAGGSVRAIKAENCATMGRKQIDKLQDFVKTYKAKGLAW
CHHHHHHHHHCCCEEEEEHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEE
IAYKEDEIKSPIAKFLTEEEMKAILEKMDAKAGDLILIVADKNNVVFESLGALRLHIAKE
EEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEHHHHCHHHHHHHHH
LDIINKNEFRFVWITEFPLLAYNEEEGRYQAEHHPFTAIMDEDIELLGTEPGKVRAKAYD
HCEECCCCEEEEEEECCCEEEEECCCCCEECCCCCEEEEECCCHHEECCCCCCEEEEEEE
IVLNGEELGGGSIRIHDSKFQEKMFSVLGFTKEKAWERFGFLLEAFKFGPPPHGGLAYGL
EEEECCCCCCCEEEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
DRMIMFLAGTENIKDVITFPKNQNAFCPLTEAPNVVDENQLEELGIKKIEKED
HHHHHHHCCCCCCHHHEECCCCCCCEECCCCCCCCCCCHHHHHHCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA