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The map label for this gene is purN [H]

Identifier: 148380832

GI number: 148380832

Start: 3058446

End: 3059063

Strand: Reverse

Name: purN [H]

Synonym: CBO2874

Alternate gene names: 148380832

Gene position: 3059063-3058446 (Counterclockwise)

Preceding gene: 148380833

Following gene: 148380831

Centisome position: 78.7

GC content: 29.29

Gene sequence:

>618_bases
ATGTTTAAAATTGCAGTACTTGTTTCAGGGGGAGGAAGCAATCTTCAATCTATAATAGATAAAATAGAAGAGGGATATAT
AAAAAATTGCAAGATAGAAATGGTTATAGGGGATAGATCTAATATTTATGGTATAGAAAGAGCAGAAAAAAAGGGAATTA
AAACTTTAACTCTAGATAGAAAAATATATAAAAGCAATCTGTCTAATAAAATATGTGAGTGTTTATATGGAAACGTAGAT
TTAATAGTTTTAGCAGGATGGCTTTCTATATTAAATGGGGATTTGGTAAATAAATTTGAAAATAAGATAATAAACATACA
TCCTTCATTAATACCAAGCTTTTGCGGAGATGGAATGTATGGTATAAAAGTGCACCAAAAAGCATTAGAATACGGAGTAA
AAGTATCTGGGTGCACTGTTCATTTTGTAGATGAGAGTACGGACAGTGGACCTATAATAATACAAAAATCTGTACCAGTT
TTTGCAGAAGATACAGCAGAAATATTGCAAAAAAGAGTTCTAGAAAAAGAACATGAGGCATTACCAGAAGCTATAAAACT
TATAAGTGAAGAAAAAGTAAAATTACAAGGAAGAAAAGTTTTTATTAAAACATACTAA

Upstream 100 bases:

>100_bases
AAAATTAGTTCATAGGTTAGATCTTTAAAATAATTGATTGGGGAATTAATTATTTATAGAGTATATATGAAAAATTTTCT
TTAAATTAAAGGAGAATAAG

Downstream 100 bases:

>100_bases
ATTTATAATAATGAAAAAATTACTATTAATATAAATGAAACTAATAGAAAAATGTGTTTAGAGTAGATTAAATTTATTGA
AATATAAAAAATTGTAGTTT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MFKIAVLVSGGGSNLQSIIDKIEEGYIKNCKIEMVIGDRSNIYGIERAEKKGIKTLTLDRKIYKSNLSNKICECLYGNVD
LIVLAGWLSILNGDLVNKFENKIINIHPSLIPSFCGDGMYGIKVHQKALEYGVKVSGCTVHFVDESTDSGPIIIQKSVPV
FAEDTAEILQKRVLEKEHEALPEAIKLISEEKVKLQGRKVFIKTY

Sequences:

>Translated_205_residues
MFKIAVLVSGGGSNLQSIIDKIEEGYIKNCKIEMVIGDRSNIYGIERAEKKGIKTLTLDRKIYKSNLSNKICECLYGNVD
LIVLAGWLSILNGDLVNKFENKIINIHPSLIPSFCGDGMYGIKVHQKALEYGVKVSGCTVHFVDESTDSGPIIIQKSVPV
FAEDTAEILQKRVLEKEHEALPEAIKLISEEKVKLQGRKVFIKTY
>Mature_205_residues
MFKIAVLVSGGGSNLQSIIDKIEEGYIKNCKIEMVIGDRSNIYGIERAEKKGIKTLTLDRKIYKSNLSNKICECLYGNVD
LIVLAGWLSILNGDLVNKFENKIINIHPSLIPSFCGDGMYGIKVHQKALEYGVKVSGCTVHFVDESTDSGPIIIQKSVPV
FAEDTAEILQKRVLEKEHEALPEAIKLISEEKVKLQGRKVFIKTY

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=208, Percent_Identity=39.9038461538462, Blast_Score=154, Evalue=4e-38,
Organism=Homo sapiens, GI209869995, Length=208, Percent_Identity=39.9038461538462, Blast_Score=154, Evalue=4e-38,
Organism=Homo sapiens, GI209869993, Length=208, Percent_Identity=39.9038461538462, Blast_Score=154, Evalue=4e-38,
Organism=Escherichia coli, GI1788846, Length=207, Percent_Identity=35.7487922705314, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787483, Length=123, Percent_Identity=35.7723577235772, Blast_Score=82, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17567511, Length=185, Percent_Identity=34.0540540540541, Blast_Score=117, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6320616, Length=206, Percent_Identity=31.5533980582524, Blast_Score=86, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24582400, Length=193, Percent_Identity=36.7875647668394, Blast_Score=141, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22844; Mature: 22844

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKIAVLVSGGGSNLQSIIDKIEEGYIKNCKIEMVIGDRSNIYGIERAEKKGIKTLTLDR
CEEEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHCCCEEEEEHH
KIYKSNLSNKICECLYGNVDLIVLAGWLSILNGDLVNKFENKIINIHPSLIPSFCGDGMY
HHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHCEEEECHHHHHHHHCCCEE
GIKVHQKALEYGVKVSGCTVHFVDESTDSGPIIIQKSVPVFAEDTAEILQKRVLEKEHEA
EHHHHHHHHHHCCEECCEEEEEEECCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHH
LPEAIKLISEEKVKLQGRKVFIKTY
HHHHHHHHHHHHEEECCCEEEEEEC
>Mature Secondary Structure
MFKIAVLVSGGGSNLQSIIDKIEEGYIKNCKIEMVIGDRSNIYGIERAEKKGIKTLTLDR
CEEEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHCCCEEEEEHH
KIYKSNLSNKICECLYGNVDLIVLAGWLSILNGDLVNKFENKIINIHPSLIPSFCGDGMY
HHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHCEEEECHHHHHHHHCCCEE
GIKVHQKALEYGVKVSGCTVHFVDESTDSGPIIIQKSVPVFAEDTAEILQKRVLEKEHEA
EHHHHHHHHHHCCEECCEEEEEEECCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHH
LPEAIKLISEEKVKLQGRKVFIKTY
HHHHHHHHHHHHEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]