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The map label for this gene is purH

Identifier: 148380831

GI number: 148380831

Start: 3056733

End: 3058232

Strand: Reverse

Name: purH

Synonym: CBO2873

Alternate gene names: 148380831

Gene position: 3058232-3056733 (Counterclockwise)

Preceding gene: 148380832

Following gene: 148380830

Centisome position: 78.68

GC content: 30.73

Gene sequence:

>1500_bases
GTGATAAAAAGAGCATTAATAAGTGTATTTGATAAAACAGGAATTTTAGATTTGGCAAAGTTTTTAGAAAGTAGAGATGT
AGAAATAATATCTACAGGTGGAACTTATAAACATTTAAAAGAAAATGGAGTAAAAGTAATAGATATAGAAGAGGTAACAG
GTTTCCCAGAAATGTTAGATGGAAGAGTAAAAACATTAAATCCTTTAATTCATGGAGGCATATTAGCTATAAGAGATAAT
GAAGAGCATATGAAGGTAATAGAAGAAAAGGGCATAAACCCTATAGATATGGTAGTAGTGAATTTATATCCTTTCTTTAA
TAAAGTAGAAGAAAATTTAAGTTTTGATGAAAAAGTAGAGTTTATAGACATTGGTGGACCTACTATGATAAGAGCAGCAG
CTAAAAATTTTAAAGATGTAGTAGTACTAACAGATACTAAGGATTATGAAAATGTCATAAATGAGATAAAAGAAAATAAT
CAGGTTAATATACAAACTAGAAAAAAATTAGCAGGAAAAGTTTTCAATCTAATGTCAGCCTATGATGCAGCTATAAGTAA
TTTCTTATTAGAAGAGGAATATCCAGAATATTTAACTCTATCCTATAAAAAGAACATGGATTTAAGATATGGAGAAAATC
CTCATCAAACAGCAGCTTATTACACATCTACTGTTGGAAAATATCCTATGAAAAATTTTGAAAAGTTAAATGGAAAAGAA
TTATCCTACAATAATATAAAGGATATGGATATAGCGTGGAAAACCGTTTGTGAATTTGAAGAGGTGGCTTGTTGTGCATT
AAAACATAATACACCTTGTGGTGTGGCTATAGGGGATACTGTACAGGAAGCTTATACTAAGGCCTATGAATGTGATCCTA
TATCTATATTTGGTGGCATAGTTGCTTTTAATAGAAAAGTAGATAAGGAAACAGCAGAAAATCTTGCAAAAATATTTTTA
GAAATAGTTGTAGCACCAGATTTTGATGAAGATGCTTTAGAAGTCTTAAAAAACAAGAAAAATTTAAGGGTTATAAAATG
TGAAGAAAAATCTACAGAAGGAAAAGATATGGCAAAGGTAGATGGAGGAATATTAGTACAAAAGAGTGATAATAAATTAT
TAGAAGATACAAAAGTAGTTACAGAAAAATCTCCAACAGAGCAAGAAATGAAAGATCTTATATTTGGTATGAAAGTAGTT
AAATATGTGAAATCCAACGCTATTGTAGTAGTTAAAGATGGTATGGCAAAAGGTATTGGGGGAGGCCAAGTAAATAGAAT
ATGGGCAGCAAAGGAAGCGTTAGATAGAGCTGGAGATGGCGTAGTTTTAGCTTCAGATGCTTTTTTCCCATTTGGAGATG
TAGCAGAAGAAGCTGCAAAATGGGGAATAAAAGCTATAATTCAACCAGGCGGTTCTATAAGAGATGAAGAATCTATAAAG
GTATGTAATGAAAAAGGGATTTCTATGGTATTTACAGGAATAAGACATTTTAAACACTAG

Upstream 100 bases:

>100_bases
GTGCCACTTATATGTTTTCTTTGAAAGAAGGTATATTGGTGGCAGTTACTTATGACAGATAAATAAGATAATAAAAAATA
TTGAAACTGGGGGAGTTTAA

Downstream 100 bases:

>100_bases
ATTTAAATATTTTTTAGTTAAAGGATAAAAGTCTTAATAAGATATTCTAATACTGAGATGAAAAGAGATATTTTTTATAA
AGAAATTTCATCTCAATATT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MIKRALISVFDKTGILDLAKFLESRDVEIISTGGTYKHLKENGVKVIDIEEVTGFPEMLDGRVKTLNPLIHGGILAIRDN
EEHMKVIEEKGINPIDMVVVNLYPFFNKVEENLSFDEKVEFIDIGGPTMIRAAAKNFKDVVVLTDTKDYENVINEIKENN
QVNIQTRKKLAGKVFNLMSAYDAAISNFLLEEEYPEYLTLSYKKNMDLRYGENPHQTAAYYTSTVGKYPMKNFEKLNGKE
LSYNNIKDMDIAWKTVCEFEEVACCALKHNTPCGVAIGDTVQEAYTKAYECDPISIFGGIVAFNRKVDKETAENLAKIFL
EIVVAPDFDEDALEVLKNKKNLRVIKCEEKSTEGKDMAKVDGGILVQKSDNKLLEDTKVVTEKSPTEQEMKDLIFGMKVV
KYVKSNAIVVVKDGMAKGIGGGQVNRIWAAKEALDRAGDGVVLASDAFFPFGDVAEEAAKWGIKAIIQPGGSIRDEESIK
VCNEKGISMVFTGIRHFKH

Sequences:

>Translated_499_residues
MIKRALISVFDKTGILDLAKFLESRDVEIISTGGTYKHLKENGVKVIDIEEVTGFPEMLDGRVKTLNPLIHGGILAIRDN
EEHMKVIEEKGINPIDMVVVNLYPFFNKVEENLSFDEKVEFIDIGGPTMIRAAAKNFKDVVVLTDTKDYENVINEIKENN
QVNIQTRKKLAGKVFNLMSAYDAAISNFLLEEEYPEYLTLSYKKNMDLRYGENPHQTAAYYTSTVGKYPMKNFEKLNGKE
LSYNNIKDMDIAWKTVCEFEEVACCALKHNTPCGVAIGDTVQEAYTKAYECDPISIFGGIVAFNRKVDKETAENLAKIFL
EIVVAPDFDEDALEVLKNKKNLRVIKCEEKSTEGKDMAKVDGGILVQKSDNKLLEDTKVVTEKSPTEQEMKDLIFGMKVV
KYVKSNAIVVVKDGMAKGIGGGQVNRIWAAKEALDRAGDGVVLASDAFFPFGDVAEEAAKWGIKAIIQPGGSIRDEESIK
VCNEKGISMVFTGIRHFKH
>Mature_499_residues
MIKRALISVFDKTGILDLAKFLESRDVEIISTGGTYKHLKENGVKVIDIEEVTGFPEMLDGRVKTLNPLIHGGILAIRDN
EEHMKVIEEKGINPIDMVVVNLYPFFNKVEENLSFDEKVEFIDIGGPTMIRAAAKNFKDVVVLTDTKDYENVINEIKENN
QVNIQTRKKLAGKVFNLMSAYDAAISNFLLEEEYPEYLTLSYKKNMDLRYGENPHQTAAYYTSTVGKYPMKNFEKLNGKE
LSYNNIKDMDIAWKTVCEFEEVACCALKHNTPCGVAIGDTVQEAYTKAYECDPISIFGGIVAFNRKVDKETAENLAKIFL
EIVVAPDFDEDALEVLKNKKNLRVIKCEEKSTEGKDMAKVDGGILVQKSDNKLLEDTKVVTEKSPTEQEMKDLIFGMKVV
KYVKSNAIVVVKDGMAKGIGGGQVNRIWAAKEALDRAGDGVVLASDAFFPFGDVAEEAAKWGIKAIIQPGGSIRDEESIK
VCNEKGISMVFTGIRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=467, Percent_Identity=35.5460385438972, Blast_Score=245, Evalue=5e-65,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=46.4761904761905, Blast_Score=474, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI71985564, Length=471, Percent_Identity=35.8811040339703, Blast_Score=231, Evalue=7e-61,
Organism=Caenorhabditis elegans, GI71985574, Length=302, Percent_Identity=29.4701986754967, Blast_Score=89, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71985556, Length=60, Percent_Identity=45, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323768, Length=467, Percent_Identity=35.3319057815846, Blast_Score=253, Evalue=5e-68,
Organism=Saccharomyces cerevisiae, GI6323056, Length=466, Percent_Identity=35.4077253218884, Blast_Score=247, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24649832, Length=469, Percent_Identity=33.6886993603412, Blast_Score=247, Evalue=1e-65,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_CLOB1 (A7FXD3)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385137.1
- ProteinModelPortal:   A7FXD3
- SMR:   A7FXD3
- STRING:   A7FXD3
- GeneID:   5395203
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2838
- eggNOG:   COG0138
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- ProtClustDB:   PRK00881
- BioCyc:   CBOT441770:CLB_2838-MONOMER
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 55748; Mature: 55748

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRALISVFDKTGILDLAKFLESRDVEIISTGGTYKHLKENGVKVIDIEEVTGFPEMLD
CCHHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCCHHHHHHCCCEEEEEHHHCCCHHHHC
GRVKTLNPLIHGGILAIRDNEEHMKVIEEKGINPIDMVVVNLYPFFNKVEENLSFDEKVE
CCHHHHHHHHHCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEE
FIDIGGPTMIRAAAKNFKDVVVLTDTKDYENVINEIKENNQVNIQTRKKLAGKVFNLMSA
EEECCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHH
YDAAISNFLLEEEYPEYLTLSYKKNMDLRYGENPHQTAAYYTSTVGKYPMKNFEKLNGKE
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHCCCCC
LSYNNIKDMDIAWKTVCEFEEVACCALKHNTPCGVAIGDTVQEAYTKAYECDPISIFGGI
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHCCCCCHHHHHHH
VAFNRKVDKETAENLAKIFLEIVVAPDFDEDALEVLKNKKNLRVIKCEEKSTEGKDMAKV
HHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHCC
DGGILVQKSDNKLLEDTKVVTEKSPTEQEMKDLIFGMKVVKYVKSNAIVVVKDGMAKGIG
CCCEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
GGQVNRIWAAKEALDRAGDGVVLASDAFFPFGDVAEEAAKWGIKAIIQPGGSIRDEESIK
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCCHHH
VCNEKGISMVFTGIRHFKH
HHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIKRALISVFDKTGILDLAKFLESRDVEIISTGGTYKHLKENGVKVIDIEEVTGFPEMLD
CCHHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCCHHHHHHCCCEEEEEHHHCCCHHHHC
GRVKTLNPLIHGGILAIRDNEEHMKVIEEKGINPIDMVVVNLYPFFNKVEENLSFDEKVE
CCHHHHHHHHHCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEE
FIDIGGPTMIRAAAKNFKDVVVLTDTKDYENVINEIKENNQVNIQTRKKLAGKVFNLMSA
EEECCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHH
YDAAISNFLLEEEYPEYLTLSYKKNMDLRYGENPHQTAAYYTSTVGKYPMKNFEKLNGKE
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHCCCCC
LSYNNIKDMDIAWKTVCEFEEVACCALKHNTPCGVAIGDTVQEAYTKAYECDPISIFGGI
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHCCCCCHHHHHHH
VAFNRKVDKETAENLAKIFLEIVVAPDFDEDALEVLKNKKNLRVIKCEEKSTEGKDMAKV
HHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHCC
DGGILVQKSDNKLLEDTKVVTEKSPTEQEMKDLIFGMKVVKYVKSNAIVVVKDGMAKGIG
CCCEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
GGQVNRIWAAKEALDRAGDGVVLASDAFFPFGDVAEEAAKWGIKAIIQPGGSIRDEESIK
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCCHHH
VCNEKGISMVFTGIRHFKH
HHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA