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The map label for this gene is tsf

Identifier: 148380392

GI number: 148380392

Start: 2566326

End: 2567249

Strand: Reverse

Name: tsf

Synonym: CBO2434

Alternate gene names: 148380392

Gene position: 2567249-2566326 (Counterclockwise)

Preceding gene: 148380393

Following gene: 148380390

Centisome position: 66.05

GC content: 32.47

Gene sequence:

>924_bases
ATGATCAGTGCTAAAATGGTAAAAGATCTAAGAGAAAAAACTGGCGCAGGAATGATGGATTGTAAAAAAGCTTTAACTGA
ATGTGATGGAGATTTAGAAAAGGCTGTAGAAGTATTAAGAGAAAAAGGGCTAGCAGCCGCTGCTAAAAAATCAGGAAGAG
TAGCTGCAGAAGGTATTGTAAGCACATACATATCTGAAGACATGAAAAATGGATCAATAGTAGAATTCAACTGTGAAACA
GATTTCGTTTCAGTAAACGAATTATTTGTAGAGTTAGCAAACAATTTATCCAAACAAGCAGCTTTTTCAAACGTTTCAAC
TGCAGAAGAATTATTAGAAGAAAAATATATAGCAGATGAAAGCAAATTAGTTAAAGATGTAATAACTGAATTAATAGCTA
AATTAGGTGAAAACATGAACCTAAGAAGAATAGCTAAACTTTCAGTAGATAAAGGTGTTATAACAAGCTACATCCATGGT
GGTGGAAGAATCGGTGTTCTTGTAAAATTAGCTTGTGAAAAGGAAGATGCGAAATTAGCTGAAATAGCTAAAGATGTAGC
AATGCAAGTTGCAGCTACAAACCCATTATTCTTAAATAGAGATGGTGTTGATACGGACACTTTAGAAAAAGAAAAAGAAA
TATACAGAGTTCAAGCTTTAAACGAAGGAAAGCCAGAAAAAGTTGTTGAAAAAATGGTTATGGGAAGAATTAATAAATAT
TATAAAGAAAACTGTTTAGTTGAACAACTTTGGGTTAAAAACGGAGATTACACTATAACTAAATACTTACAAGAACAATC
AAAAGAAATCGGTGCAGATATAACTGTAGAAGCTTTCGTAAGATACGAAAAAGGTGAAGGCATAGAAAAGAAAGAAGAAG
ACTTTGCTGAAGAAGTTCAAAGACAAATGAACCAAGGCAAATAA

Upstream 100 bases:

>100_bases
ATTAGCAGAATAATTTAGCTATTATAAATGAAAAATATAAGGTAGGTGAGACTAATCTCTCATTTACCTTTTAAATTAGT
TAAAATAGGAGGAATATATA

Downstream 100 bases:

>100_bases
TTAAAAAAGAGAACACTGAGTGTTCTCTTTTTTTGAAAAATAAGTTAATAAGAAAACATATAAGATTAAGGAATTTTAAA
AATTATGAAAAGTTTTATTA

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MISAKMVKDLREKTGAGMMDCKKALTECDGDLEKAVEVLREKGLAAAAKKSGRVAAEGIVSTYISEDMKNGSIVEFNCET
DFVSVNELFVELANNLSKQAAFSNVSTAEELLEEKYIADESKLVKDVITELIAKLGENMNLRRIAKLSVDKGVITSYIHG
GGRIGVLVKLACEKEDAKLAEIAKDVAMQVAATNPLFLNRDGVDTDTLEKEKEIYRVQALNEGKPEKVVEKMVMGRINKY
YKENCLVEQLWVKNGDYTITKYLQEQSKEIGADITVEAFVRYEKGEGIEKKEEDFAEEVQRQMNQGK

Sequences:

>Translated_307_residues
MISAKMVKDLREKTGAGMMDCKKALTECDGDLEKAVEVLREKGLAAAAKKSGRVAAEGIVSTYISEDMKNGSIVEFNCET
DFVSVNELFVELANNLSKQAAFSNVSTAEELLEEKYIADESKLVKDVITELIAKLGENMNLRRIAKLSVDKGVITSYIHG
GGRIGVLVKLACEKEDAKLAEIAKDVAMQVAATNPLFLNRDGVDTDTLEKEKEIYRVQALNEGKPEKVVEKMVMGRINKY
YKENCLVEQLWVKNGDYTITKYLQEQSKEIGADITVEAFVRYEKGEGIEKKEEDFAEEVQRQMNQGK
>Mature_307_residues
MISAKMVKDLREKTGAGMMDCKKALTECDGDLEKAVEVLREKGLAAAAKKSGRVAAEGIVSTYISEDMKNGSIVEFNCET
DFVSVNELFVELANNLSKQAAFSNVSTAEELLEEKYIADESKLVKDVITELIAKLGENMNLRRIAKLSVDKGVITSYIHG
GGRIGVLVKLACEKEDAKLAEIAKDVAMQVAATNPLFLNRDGVDTDTLEKEKEIYRVQALNEGKPEKVVEKMVMGRINKY
YKENCLVEQLWVKNGDYTITKYLQEQSKEIGADITVEAFVRYEKGEGIEKKEEDFAEEVQRQMNQGK

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Homo sapiens, GI171846268, Length=224, Percent_Identity=31.25, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1786366, Length=307, Percent_Identity=42.3452768729642, Blast_Score=210, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI17561440, Length=308, Percent_Identity=27.9220779220779, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_CLOB1 (A7FPZ7)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001384609.1
- ProteinModelPortal:   A7FPZ7
- SMR:   A7FPZ7
- STRING:   A7FPZ7
- GeneID:   5397652
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2298
- eggNOG:   COG0264
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- ProtClustDB:   PRK09377
- BioCyc:   CBOT441770:CLB_2298-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 34200; Mature: 34200

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISAKMVKDLREKTGAGMMDCKKALTECDGDLEKAVEVLREKGLAAAAKKSGRVAAEGIV
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHHHH
STYISEDMKNGSIVEFNCETDFVSVNELFVELANNLSKQAAFSNVSTAEELLEEKYIADE
HHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
SKLVKDVITELIAKLGENMNLRRIAKLSVDKGVITSYIHGGGRIGVLVKLACEKEDAKLA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHCCCCEEEEEEEECCCCCHHHH
EIAKDVAMQVAATNPLFLNRDGVDTDTLEKEKEIYRVQALNEGKPEKVVEKMVMGRINKY
HHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
YKENCLVEQLWVKNGDYTITKYLQEQSKEIGADITVEAFVRYEKGEGIEKKEEDFAEEVQ
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHEECCCCCCCHHHHHHHHHHH
RQMNQGK
HHHHCCC
>Mature Secondary Structure
MISAKMVKDLREKTGAGMMDCKKALTECDGDLEKAVEVLREKGLAAAAKKSGRVAAEGIV
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHHHH
STYISEDMKNGSIVEFNCETDFVSVNELFVELANNLSKQAAFSNVSTAEELLEEKYIADE
HHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
SKLVKDVITELIAKLGENMNLRRIAKLSVDKGVITSYIHGGGRIGVLVKLACEKEDAKLA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHCCCCEEEEEEEECCCCCHHHH
EIAKDVAMQVAATNPLFLNRDGVDTDTLEKEKEIYRVQALNEGKPEKVVEKMVMGRINKY
HHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
YKENCLVEQLWVKNGDYTITKYLQEQSKEIGADITVEAFVRYEKGEGIEKKEEDFAEEVQ
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHEECCCCCCCHHHHHHHHHHH
RQMNQGK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA