Click here to switch to the map view.

The map label for this gene is pyrH

Identifier: 148380390

GI number: 148380390

Start: 2565235

End: 2565951

Strand: Reverse

Name: pyrH

Synonym: CBO2433

Alternate gene names: 148380390

Gene position: 2565951-2565235 (Counterclockwise)

Preceding gene: 148380392

Following gene: 148380389

Centisome position: 66.02

GC content: 32.91

Gene sequence:

>717_bases
ATGAATGAACCAAAGTATAAGCGCGTTATGTTAAAGCTATCAGGAGAAGCTTTGTCAGGGGAGAAGGGTTTTGGATTTGA
TTTTGATTTTACCAAGGAAATATCAGAACAAATAAAAAAATTAGTTGACATGGGAATAGAAGTAGGCGCAGTAGTTGGTG
GAGGAAACATTTGGAGAGGCAGAAGTGGTTCAGAAATGGATAGAACTACCGCAGATTATATGGGAATGTTAGCTACTTGT
ATAAATGCTTTAGCCCTTCAAGATTCATTAGAACAACTTGGAGTTAATACTAGAGTTCAAACTGCTATAGAAATGAAAGA
AATTGCAGAACCATTTATAAGAAGAAGAGCTATGAGACACTTAGAAAAAGAAAGAGTGGTAATATTTGCATCTGGAACAG
GTAATCCATATTTTTCAACTGATACAGCAGCTGCATTAAGAGCTGCAGAAATAGAAGCAGATGTTATATTACTTGCTAAA
AAAGTAGATGGGGTTTATGATAAGGACCCTCATAAGTATGATGATGCTAAAAAATATAATAAATTATCTTATATAGAAGT
ATTAGAACAAGGATTACAAGTGATGGATTCTACAGCTACTTCTTTATGTATGGATAATGATATTCCTATATTAGTTTTTG
GATTAGATGAACCTTGCAATATAATAAAAGCTGTAACTGGAGAAGAAATAGGTACTCTAGTTTCAAATTCAAAATAA

Upstream 100 bases:

>100_bases
TCTATTGTGTTTTATAGAAATATTAGATTTCTTTTAATCTAGAATCGAAGTTATAATACATAATATATTTAATTTAAAAC
AATTTGGAGGTATAATATAG

Downstream 100 bases:

>100_bases
GGGGAGGTTATTTAAGTGATAAAAGAAATATTAAAAAAAGCAGATGAAAAAATGGGTAAAACTATTGTAGCTTTAAAAAA
AGAATTAGCTTCAATGAAAG

Product: uridylate kinase

Products: NA

Alternate protein names: UK; Uridine monophosphate kinase; UMP kinase; UMPK

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MNEPKYKRVMLKLSGEALSGEKGFGFDFDFTKEISEQIKKLVDMGIEVGAVVGGGNIWRGRSGSEMDRTTADYMGMLATC
INALALQDSLEQLGVNTRVQTAIEMKEIAEPFIRRRAMRHLEKERVVIFASGTGNPYFSTDTAAALRAAEIEADVILLAK
KVDGVYDKDPHKYDDAKKYNKLSYIEVLEQGLQVMDSTATSLCMDNDIPILVFGLDEPCNIIKAVTGEEIGTLVSNSK

Sequences:

>Translated_238_residues
MNEPKYKRVMLKLSGEALSGEKGFGFDFDFTKEISEQIKKLVDMGIEVGAVVGGGNIWRGRSGSEMDRTTADYMGMLATC
INALALQDSLEQLGVNTRVQTAIEMKEIAEPFIRRRAMRHLEKERVVIFASGTGNPYFSTDTAAALRAAEIEADVILLAK
KVDGVYDKDPHKYDDAKKYNKLSYIEVLEQGLQVMDSTATSLCMDNDIPILVFGLDEPCNIIKAVTGEEIGTLVSNSK
>Mature_238_residues
MNEPKYKRVMLKLSGEALSGEKGFGFDFDFTKEISEQIKKLVDMGIEVGAVVGGGNIWRGRSGSEMDRTTADYMGMLATC
INALALQDSLEQLGVNTRVQTAIEMKEIAEPFIRRRAMRHLEKERVVIFASGTGNPYFSTDTAAALRAAEIEADVILLAK
KVDGVYDKDPHKYDDAKKYNKLSYIEVLEQGLQVMDSTATSLCMDNDIPILVFGLDEPCNIIKAVTGEEIGTLVSNSK

Specific function: Catalyzes the reversible phosphorylation of UMP to UDP

COG id: COG0528

COG function: function code F; Uridylate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UMP kinase family

Homologues:

Organism=Escherichia coli, GI1786367, Length=235, Percent_Identity=47.2340425531915, Blast_Score=226, Evalue=7e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRH_CLOB1 (A7FPZ6)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001384608.1
- ProteinModelPortal:   A7FPZ6
- SMR:   A7FPZ6
- STRING:   A7FPZ6
- GeneID:   5395276
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2297
- eggNOG:   COG0528
- HOGENOM:   HBG497552
- OMA:   RHMEKGR
- ProtClustDB:   PRK00358
- BioCyc:   CBOT441770:CLB_2297-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01220_B
- InterPro:   IPR001048
- InterPro:   IPR011817
- InterPro:   IPR015963
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF005650
- TIGRFAMs:   TIGR02075

Pfam domain/function: PF00696 AA_kinase; SSF53633 Aa_kinase

EC number: =2.7.4.22

Molecular weight: Translated: 26243; Mature: 26243

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: BINDING 54-54 BINDING 55-55 BINDING 59-59 BINDING 72-72 BINDING 166-166 BINDING 169-169

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEPKYKRVMLKLSGEALSGEKGFGFDFDFTKEISEQIKKLVDMGIEVGAVVGGGNIWRG
CCCCHHHEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEECC
RSGSEMDRTTADYMGMLATCINALALQDSLEQLGVNTRVQTAIEMKEIAEPFIRRRAMRH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LEKERVVIFASGTGNPYFSTDTAAALRAAEIEADVILLAKKVDGVYDKDPHKYDDAKKYN
HHHCCEEEEECCCCCCCCCCHHHHHHHHHHCCHHHEEEEHHHCCCCCCCCCCCCHHHHHH
KLSYIEVLEQGLQVMDSTATSLCMDNDIPILVFGLDEPCNIIKAVTGEEIGTLVSNSK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCHHHHHHHCCCC
>Mature Secondary Structure
MNEPKYKRVMLKLSGEALSGEKGFGFDFDFTKEISEQIKKLVDMGIEVGAVVGGGNIWRG
CCCCHHHEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEECC
RSGSEMDRTTADYMGMLATCINALALQDSLEQLGVNTRVQTAIEMKEIAEPFIRRRAMRH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LEKERVVIFASGTGNPYFSTDTAAALRAAEIEADVILLAKKVDGVYDKDPHKYDDAKKYN
HHHCCEEEEECCCCCCCCCCHHHHHHHHHHCCHHHEEEEHHHCCCCCCCCCCCCHHHHHH
KLSYIEVLEQGLQVMDSTATSLCMDNDIPILVFGLDEPCNIIKAVTGEEIGTLVSNSK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA