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The map label for this gene is cssS [H]

Identifier: 148378298

GI number: 148378298

Start: 347941

End: 349326

Strand: Direct

Name: cssS [H]

Synonym: CBO0296

Alternate gene names: 148378298

Gene position: 347941-349326 (Clockwise)

Preceding gene: 148378297

Following gene: 148378299

Centisome position: 8.95

GC content: 25.54

Gene sequence:

>1386_bases
ATGAAGAGATTAAGATTTAAATCATTAACAATGAGGATATGGACAACATTCACTGCTATAATTTTAATAATAATATGTAG
TATTTCATTTTTATATTTAGTTGCCTTTAAGAGAATTAGTGAAAATTCAAGGTTAGAGGATTTAGAAGTCGCTCATGATG
TTTTATTAAACAGCAATAATCTTAATGAACAAAATAGATTTGATGAATTAAAAAATCTTAAGGGAAGTGATCATTTTATT
GTAAAAATAGATGAGAATGATAAATATAAAATTATAGATATAGGTAAAAGAAAAGAGGAACCACCACCAATAGAAAAGAA
GAATCCACCTCCAGGACCTGCTAGTGATGGAATAAAAATGTGGATGGCTAGTTATATTATAGGTAATAATATTCAGCAAA
AGCAATTTAAAGAATATCATAACAACATGAAATTTATTTTTATAATAAGTTCAATTAAAAATACAGGAGGGGAAAAACTT
TATTTAATATCGTATATACCCGAAATACAAGATAATGTATTACTATATACAGTAATAATTATAGGTATTATATTTATTGG
TATAGGATTTTTTACTGCTAAATTAGTTGCAAATTATATTTCAAGGCCTTTAAGAGAGCTTGAGGATTATACTGTAAAAA
TAGCTCATAAGGATTGGAGGGAGCCTATTAAAATTAAAAATGATGATGAGATAGGTAGATTGGTAGATTCAATGAATCGT
ATGCAAAAAGAATTAAAAAAGATTGATGAAGAAGAAAAAATGTTTCTACAAAGCATATCTCATGATTTAAAGACTCCAGT
AATGGTAATTATGAGCCATGCTCAAGCTATTATTGATGGTATGTACATAGAATCAGTTGAAGAAACTGCTGAGATAATAA
AAGATGAGGCAGCTATTCTTGAGAAAAAAATTAAACAATTATTGTATTTAAATACTCTTGCCTATACTTTAGAAAATGAT
AGTGAAAATATAGGATTTGAGTTACATAATTTATTGTTTAATATTATAAGTAGATTTGAGATAGTTAATAGTAAAATTCA
ATGGAATTTAGATATTGATAAGGTTGTTATAAAAGGAAATGCTGAAAAAATACAAGTAGCTATTGAAAATATATTAGATA
ATGGACTTAGGTATGCAGAAAGAAAAATTGCCATAACTCTTAAAAAAGAAAATTCTGTTGCAGTATTAGAAATTTATAAT
GATGGACCTAATATTGATAAAAATAATATAGAGCACATTTTTGAAAATCTATATAAAGATAGGACTGGAAATTTTGGCCT
AGGTTTAGCTATTTCTAAAAAAATTATAGATTTTTATAATGGAGAAATAAAGGCAGTAAATAGAGATAATGGTGTAAGTT
TTATAATTAAATATCCTATAGATTAA

Upstream 100 bases:

>100_bases
ATAGAGTTATTGATGATACAATTAGGAGATTGCGCAAAAAGATGGATAAGTTAGATCTTGAAACTGTATATGGATATGGT
TATAAATTGGTGGTTAAATG

Downstream 100 bases:

>100_bases
TAATTGTCTCTCGGGAAAGTAAAAGTAATTATTATTTACACTTTCCAATAAATTTTAATAATTAAATTTAGCCCTTAGGT
AAGTATAAATAAGATTTATT

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKRLRFKSLTMRIWTTFTAIILIIICSISFLYLVAFKRISENSRLEDLEVAHDVLLNSNNLNEQNRFDELKNLKGSDHFI
VKIDENDKYKIIDIGKRKEEPPPIEKKNPPPGPASDGIKMWMASYIIGNNIQQKQFKEYHNNMKFIFIISSIKNTGGEKL
YLISYIPEIQDNVLLYTVIIIGIIFIGIGFFTAKLVANYISRPLRELEDYTVKIAHKDWREPIKIKNDDEIGRLVDSMNR
MQKELKKIDEEEKMFLQSISHDLKTPVMVIMSHAQAIIDGMYIESVEETAEIIKDEAAILEKKIKQLLYLNTLAYTLEND
SENIGFELHNLLFNIISRFEIVNSKIQWNLDIDKVVIKGNAEKIQVAIENILDNGLRYAERKIAITLKKENSVAVLEIYN
DGPNIDKNNIEHIFENLYKDRTGNFGLGLAISKKIIDFYNGEIKAVNRDNGVSFIIKYPID

Sequences:

>Translated_461_residues
MKRLRFKSLTMRIWTTFTAIILIIICSISFLYLVAFKRISENSRLEDLEVAHDVLLNSNNLNEQNRFDELKNLKGSDHFI
VKIDENDKYKIIDIGKRKEEPPPIEKKNPPPGPASDGIKMWMASYIIGNNIQQKQFKEYHNNMKFIFIISSIKNTGGEKL
YLISYIPEIQDNVLLYTVIIIGIIFIGIGFFTAKLVANYISRPLRELEDYTVKIAHKDWREPIKIKNDDEIGRLVDSMNR
MQKELKKIDEEEKMFLQSISHDLKTPVMVIMSHAQAIIDGMYIESVEETAEIIKDEAAILEKKIKQLLYLNTLAYTLEND
SENIGFELHNLLFNIISRFEIVNSKIQWNLDIDKVVIKGNAEKIQVAIENILDNGLRYAERKIAITLKKENSVAVLEIYN
DGPNIDKNNIEHIFENLYKDRTGNFGLGLAISKKIIDFYNGEIKAVNRDNGVSFIIKYPID
>Mature_461_residues
MKRLRFKSLTMRIWTTFTAIILIIICSISFLYLVAFKRISENSRLEDLEVAHDVLLNSNNLNEQNRFDELKNLKGSDHFI
VKIDENDKYKIIDIGKRKEEPPPIEKKNPPPGPASDGIKMWMASYIIGNNIQQKQFKEYHNNMKFIFIISSIKNTGGEKL
YLISYIPEIQDNVLLYTVIIIGIIFIGIGFFTAKLVANYISRPLRELEDYTVKIAHKDWREPIKIKNDDEIGRLVDSMNR
MQKELKKIDEEEKMFLQSISHDLKTPVMVIMSHAQAIIDGMYIESVEETAEIIKDEAAILEKKIKQLLYLNTLAYTLEND
SENIGFELHNLLFNIISRFEIVNSKIQWNLDIDKVVIKGNAEKIQVAIENILDNGLRYAERKIAITLKKENSVAVLEIYN
DGPNIDKNNIEHIFENLYKDRTGNFGLGLAISKKIIDFYNGEIKAVNRDNGVSFIIKYPID

Specific function: Member of the two-component regulatory system CssS/CssR required to control the cellular response to secretion stress. Required for the transcription of htrA. Could detect misfolded proteins at the membrane-cell wall interface and then activate CssR by ph

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=273, Percent_Identity=26.7399267399267, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789808, Length=290, Percent_Identity=24.4827586206897, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790551, Length=289, Percent_Identity=21.7993079584775, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1790346, Length=269, Percent_Identity=26.0223048327138, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081816, Length=335, Percent_Identity=20.8955223880597, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1790861, Length=314, Percent_Identity=21.9745222929936, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1787374, Length=283, Percent_Identity=23.6749116607774, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53311; Mature: 53311

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLRFKSLTMRIWTTFTAIILIIICSISFLYLVAFKRISENSRLEDLEVAHDVLLNSNN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCC
LNEQNRFDELKNLKGSDHFIVKIDENDKYKIIDIGKRKEEPPPIEKKNPPPGPASDGIKM
CCHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHH
WMASYIIGNNIQQKQFKEYHNNMKFIFIISSIKNTGGEKLYLISYIPEIQDNVLLYTVII
HHHHHHHCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHH
IGIIFIGIGFFTAKLVANYISRPLRELEDYTVKIAHKDWREPIKIKNDDEIGRLVDSMNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
MQKELKKIDEEEKMFLQSISHDLKTPVMVIMSHAQAIIDGMYIESVEETAEIIKDEAAIL
HHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKKIKQLLYLNTLAYTLENDSENIGFELHNLLFNIISRFEIVNSKIQWNLDIDKVVIKGN
HHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECC
AEKIQVAIENILDNGLRYAERKIAITLKKENSVAVLEIYNDGPNIDKNNIEHIFENLYKD
HHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHC
RTGNFGLGLAISKKIIDFYNGEIKAVNRDNGVSFIIKYPID
CCCCCCEEHHHHHHHHHHHCCEEEEEECCCCEEEEEECCCC
>Mature Secondary Structure
MKRLRFKSLTMRIWTTFTAIILIIICSISFLYLVAFKRISENSRLEDLEVAHDVLLNSNN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCC
LNEQNRFDELKNLKGSDHFIVKIDENDKYKIIDIGKRKEEPPPIEKKNPPPGPASDGIKM
CCHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHH
WMASYIIGNNIQQKQFKEYHNNMKFIFIISSIKNTGGEKLYLISYIPEIQDNVLLYTVII
HHHHHHHCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHH
IGIIFIGIGFFTAKLVANYISRPLRELEDYTVKIAHKDWREPIKIKNDDEIGRLVDSMNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
MQKELKKIDEEEKMFLQSISHDLKTPVMVIMSHAQAIIDGMYIESVEETAEIIKDEAAIL
HHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKKIKQLLYLNTLAYTLENDSENIGFELHNLLFNIISRFEIVNSKIQWNLDIDKVVIKGN
HHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECC
AEKIQVAIENILDNGLRYAERKIAITLKKENSVAVLEIYNDGPNIDKNNIEHIFENLYKD
HHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHC
RTGNFGLGLAISKKIIDFYNGEIKAVNRDNGVSFIIKYPID
CCCCCCEEHHHHHHHHHHHCCEEEEEECCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353931; 9639930; 9384377; 11555295 [H]