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The map label for this gene is priA [H]

Identifier: 148361177

GI number: 148361177

Start: 3410438

End: 3412615

Strand: Direct

Name: priA [H]

Synonym: LPC_3151

Alternate gene names: 148361177

Gene position: 3410438-3412615 (Clockwise)

Preceding gene: 148361176

Following gene: 148361178

Centisome position: 95.36

GC content: 41.32

Gene sequence:

>2178_bases
ATGAACGATATTTATCAAGTTTGTATCCCCCATACCAATCGAGATTGTTTTGATTACGAAGCCCTGGATTTAACTCCATG
CATTGGCGGAAGAGTATGGGTTCCTTTTCGCAATCAAGCCCGAGTGGGCATTGTAATCAATAAAGTTTGTGGCCAGCAAA
CGGGCCATTCGTTAAAGAAAATCACTTCAATAATCGATGAGAAACCTCTGGTTCCTGAAGATCTCTTAGAGCTTTGTCTA
TGGGTAGGAAGTTATTATCAGTCACCATTATCTGAAGTGATTCCTTTGGTAATACCCAAAAAATATCGCCTGGGTTTGCC
TTGCTCACTACCTACAGAAGATTTTTATCAATTGAATATCCCTGTTGAAAAGGCGAAGACACTCATCTCGAAAAAAGCAA
AAAAACAACATGAATTGATTGATTTTTTAAGCAGGCACCAGAAAGGAATTTCCAAACAGACCCTAAAACAACATGGCTAT
AACGGAACTCAAATTGCGGCACTACTTGCTTGTCAAGCCATTAACCTGTCACAGCAAGTTATCTTGCCAACCAAAATTCC
TGAAAAACTTAGTCCACCGTTAGCACTCAATCCTGAACAAGCCGTTGCCGTAGCTTCCATTTTCGAACACATCCATCATT
ACCAATGCTTTTTACTGCAAGGTGTTACCGGAAGTGGTAAGACAGAGGTTTATTTGCATGTCATCGCCAAAATATTGGAG
CAAGGCAAACAAGTATTGGTGCTGGTACCAGAAATCGGGTTAACCCCTCAGCTTTTATCTCGTTTTACAGCCCGATTTAA
CCATCCCATAACCGTATTACATTCCAATTTGAATGAATCAGAAAGGCAAATTGCCTGGCAGCTGGCAAAAGAGAATAAAG
TTAAAATGGTGATTGGAACTCGTTCTGCCGTTTTTACTCCTCTGCCCAATCTCGGTTTAATTATCATCGATGAAGAGCAT
GACAGTTCACTCAAACAAATGGAGGGGGTGCGTTATTCTGCGCGAGATACTGCTTTAATGCGTGCTCATCTTGCCAATAT
TCCTATCATTCTTGGTACTGCCACTCCCAGTCTGGAAACCATCCACAACTGCAAACAAAAAAAATATAATTTATTACGCT
TAACGCATAAAGCTGCCTCCACTACGCCATTACACTATCAACTGATTGATTTAAGATCGCAAATGGTACAGCATGGCTTG
GCTCTCCCAACCCTTAAAATCATAGAAAATCATTTGCTGCAACAAAACCAGGTACTTGTTTTCATTAACCGACGCGGCTT
TGCTCCAGTATTACTCTGCCATCAATGTGGATGGATGGTTGACTGCAAAGCATGCGATAGCCATTTAACCTTGCATAAAC
AGGCAGGACAAATGATTTGTCATCATTGCGGATTAACCCAAAGAATACCCTTGTTTTGCAATAACTGCCAAAGCAAGGAA
TTAATCCCCGTAGGATCTGGCACCCAGAGAATTTATGAATTTTTAAGCCATCAGTTTCCAGATACGAACGTTTTACGAAT
TGACCGAGATGAGGTTCGTAAAAAAAATTCTCTAAACAGCCATTTGGATAAAATTAATAAAGGGGAAGCTCAGCTGATCA
TAGGCACTCAAATGCTAGCCAAGGGACACCATTTTCCCCGCCTATCCCTGGTCGTTGTCGTAGATGCTGATGCGGGTTTT
TACAACCAGGATTTCCGGGCTATTGAACATTTGGGACAATTATTAACTCAAGTATCTGGACGAGCTGGCAGAGCAGAACA
TGCCGGGCAAGTATTGATTCAAACTTATTTACCTGATCATCCCCTGCTCAATCTACTCATTCAGCATGGGTATGATGAAT
TTGCAAACGCTTTATTAATTTCAAGACAAGAGGCTGAAATGCCCCCTTTTCAGTTTTTGGCGGTCATCAGAGCTCAGGGC
AAAACCGTCAACAAGGTGGCTCAATTCCTAAAAGCAACCAAAGATCAAATTCAGGCCCATCCTCTCAAGGTATTAGGCCC
TGCGCCAGCTCCTTTGCCACGAAAAGCTAACCAGCATAGAATGCAACTATTAATTAAATCCCCTTCAAGAAAAGTTCTAA
AAAGTTCATTGACGCAATTGAGAGAGTGGTTAACAATGAATAAATTAAGTAATGGCATCCGCTGGAATGTGGATGTTGAT
CCTATGGACTTATCATGA

Upstream 100 bases:

>100_bases
AGAAAAAGAGGAGTTAGATAGACATCGCATCGAAAAAGTAGTAGTCAAAGTGTTTTCCGCGCCCGGCCAATCAGCGAGTC
ATGAATGGCTTAGAAGTTAA

Downstream 100 bases:

>100_bases
CTGAAATAAACAAAATAATACATAAAAAAACTTTTGATATCGCTTGGGGCGATATGGATGCATTAGGACATGTTAACAAT
GCTCGTTATTTTGACTATTT

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 725; Mature: 725

Protein sequence:

>725_residues
MNDIYQVCIPHTNRDCFDYEALDLTPCIGGRVWVPFRNQARVGIVINKVCGQQTGHSLKKITSIIDEKPLVPEDLLELCL
WVGSYYQSPLSEVIPLVIPKKYRLGLPCSLPTEDFYQLNIPVEKAKTLISKKAKKQHELIDFLSRHQKGISKQTLKQHGY
NGTQIAALLACQAINLSQQVILPTKIPEKLSPPLALNPEQAVAVASIFEHIHHYQCFLLQGVTGSGKTEVYLHVIAKILE
QGKQVLVLVPEIGLTPQLLSRFTARFNHPITVLHSNLNESERQIAWQLAKENKVKMVIGTRSAVFTPLPNLGLIIIDEEH
DSSLKQMEGVRYSARDTALMRAHLANIPIILGTATPSLETIHNCKQKKYNLLRLTHKAASTTPLHYQLIDLRSQMVQHGL
ALPTLKIIENHLLQQNQVLVFINRRGFAPVLLCHQCGWMVDCKACDSHLTLHKQAGQMICHHCGLTQRIPLFCNNCQSKE
LIPVGSGTQRIYEFLSHQFPDTNVLRIDRDEVRKKNSLNSHLDKINKGEAQLIIGTQMLAKGHHFPRLSLVVVVDADAGF
YNQDFRAIEHLGQLLTQVSGRAGRAEHAGQVLIQTYLPDHPLLNLLIQHGYDEFANALLISRQEAEMPPFQFLAVIRAQG
KTVNKVAQFLKATKDQIQAHPLKVLGPAPAPLPRKANQHRMQLLIKSPSRKVLKSSLTQLREWLTMNKLSNGIRWNVDVD
PMDLS

Sequences:

>Translated_725_residues
MNDIYQVCIPHTNRDCFDYEALDLTPCIGGRVWVPFRNQARVGIVINKVCGQQTGHSLKKITSIIDEKPLVPEDLLELCL
WVGSYYQSPLSEVIPLVIPKKYRLGLPCSLPTEDFYQLNIPVEKAKTLISKKAKKQHELIDFLSRHQKGISKQTLKQHGY
NGTQIAALLACQAINLSQQVILPTKIPEKLSPPLALNPEQAVAVASIFEHIHHYQCFLLQGVTGSGKTEVYLHVIAKILE
QGKQVLVLVPEIGLTPQLLSRFTARFNHPITVLHSNLNESERQIAWQLAKENKVKMVIGTRSAVFTPLPNLGLIIIDEEH
DSSLKQMEGVRYSARDTALMRAHLANIPIILGTATPSLETIHNCKQKKYNLLRLTHKAASTTPLHYQLIDLRSQMVQHGL
ALPTLKIIENHLLQQNQVLVFINRRGFAPVLLCHQCGWMVDCKACDSHLTLHKQAGQMICHHCGLTQRIPLFCNNCQSKE
LIPVGSGTQRIYEFLSHQFPDTNVLRIDRDEVRKKNSLNSHLDKINKGEAQLIIGTQMLAKGHHFPRLSLVVVVDADAGF
YNQDFRAIEHLGQLLTQVSGRAGRAEHAGQVLIQTYLPDHPLLNLLIQHGYDEFANALLISRQEAEMPPFQFLAVIRAQG
KTVNKVAQFLKATKDQIQAHPLKVLGPAPAPLPRKANQHRMQLLIKSPSRKVLKSSLTQLREWLTMNKLSNGIRWNVDVD
PMDLS
>Mature_725_residues
MNDIYQVCIPHTNRDCFDYEALDLTPCIGGRVWVPFRNQARVGIVINKVCGQQTGHSLKKITSIIDEKPLVPEDLLELCL
WVGSYYQSPLSEVIPLVIPKKYRLGLPCSLPTEDFYQLNIPVEKAKTLISKKAKKQHELIDFLSRHQKGISKQTLKQHGY
NGTQIAALLACQAINLSQQVILPTKIPEKLSPPLALNPEQAVAVASIFEHIHHYQCFLLQGVTGSGKTEVYLHVIAKILE
QGKQVLVLVPEIGLTPQLLSRFTARFNHPITVLHSNLNESERQIAWQLAKENKVKMVIGTRSAVFTPLPNLGLIIIDEEH
DSSLKQMEGVRYSARDTALMRAHLANIPIILGTATPSLETIHNCKQKKYNLLRLTHKAASTTPLHYQLIDLRSQMVQHGL
ALPTLKIIENHLLQQNQVLVFINRRGFAPVLLCHQCGWMVDCKACDSHLTLHKQAGQMICHHCGLTQRIPLFCNNCQSKE
LIPVGSGTQRIYEFLSHQFPDTNVLRIDRDEVRKKNSLNSHLDKINKGEAQLIIGTQMLAKGHHFPRLSLVVVVDADAGF
YNQDFRAIEHLGQLLTQVSGRAGRAEHAGQVLIQTYLPDHPLLNLLIQHGYDEFANALLISRQEAEMPPFQFLAVIRAQG
KTVNKVAQFLKATKDQIQAHPLKVLGPAPAPLPRKANQHRMQLLIKSPSRKVLKSSLTQLREWLTMNKLSNGIRWNVDVD
PMDLS

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=736, Percent_Identity=41.5760869565217, Blast_Score=557, Evalue=1e-160,
Organism=Escherichia coli, GI1787357, Length=179, Percent_Identity=30.7262569832402, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81552; Mature: 81552

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDIYQVCIPHTNRDCFDYEALDLTPCIGGRVWVPFRNQARVGIVINKVCGQQTGHSLKK
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEHHHCCCCCHHHHH
ITSIIDEKPLVPEDLLELCLWVGSYYQSPLSEVIPLVIPKKYRLGLPCSLPTEDFYQLNI
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEECC
PVEKAKTLISKKAKKQHELIDFLSRHQKGISKQTLKQHGYNGTQIAALLACQAINLSQQV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCEE
ILPTKIPEKLSPPLALNPEQAVAVASIFEHIHHYQCFLLQGVTGSGKTEVYLHVIAKILE
EECCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHH
QGKQVLVLVPEIGLTPQLLSRFTARFNHPITVLHSNLNESERQIAWQLAKENKVKMVIGT
CCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEC
RSAVFTPLPNLGLIIIDEEHDSSLKQMEGVRYSARDTALMRAHLANIPIILGTATPSLET
CCCEECCCCCCCEEEEECCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHH
IHNCKQKKYNLLRLTHKAASTTPLHYQLIDLRSQMVQHGLALPTLKIIENHLLQQNQVLV
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEE
FINRRGFAPVLLCHQCGWMVDCKACDSHLTLHKQAGQMICHHCGLTQRIPLFCNNCQSKE
EEECCCCCHHHHHHHCCCEEEHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCC
LIPVGSGTQRIYEFLSHQFPDTNVLRIDRDEVRKKNSLNSHLDKINKGEAQLIIGTQMLA
EEECCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHH
KGHHFPRLSLVVVVDADAGFYNQDFRAIEHLGQLLTQVSGRAGRAEHAGQVLIQTYLPDH
CCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEECCCCC
PLLNLLIQHGYDEFANALLISRQEAEMPPFQFLAVIRAQGKTVNKVAQFLKATKDQIQAH
HHHHHHHHCCHHHHHHHHHHCCHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
PLKVLGPAPAPLPRKANQHRMQLLIKSPSRKVLKSSLTQLREWLTMNKLSNGIRWNVDVD
CEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
PMDLS
CCCCC
>Mature Secondary Structure
MNDIYQVCIPHTNRDCFDYEALDLTPCIGGRVWVPFRNQARVGIVINKVCGQQTGHSLKK
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEHHHCCCCCHHHHH
ITSIIDEKPLVPEDLLELCLWVGSYYQSPLSEVIPLVIPKKYRLGLPCSLPTEDFYQLNI
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEECC
PVEKAKTLISKKAKKQHELIDFLSRHQKGISKQTLKQHGYNGTQIAALLACQAINLSQQV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCEE
ILPTKIPEKLSPPLALNPEQAVAVASIFEHIHHYQCFLLQGVTGSGKTEVYLHVIAKILE
EECCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHH
QGKQVLVLVPEIGLTPQLLSRFTARFNHPITVLHSNLNESERQIAWQLAKENKVKMVIGT
CCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEC
RSAVFTPLPNLGLIIIDEEHDSSLKQMEGVRYSARDTALMRAHLANIPIILGTATPSLET
CCCEECCCCCCCEEEEECCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHH
IHNCKQKKYNLLRLTHKAASTTPLHYQLIDLRSQMVQHGLALPTLKIIENHLLQQNQVLV
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEE
FINRRGFAPVLLCHQCGWMVDCKACDSHLTLHKQAGQMICHHCGLTQRIPLFCNNCQSKE
EEECCCCCHHHHHHHCCCEEEHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCC
LIPVGSGTQRIYEFLSHQFPDTNVLRIDRDEVRKKNSLNSHLDKINKGEAQLIIGTQMLA
EEECCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHH
KGHHFPRLSLVVVVDADAGFYNQDFRAIEHLGQLLTQVSGRAGRAEHAGQVLIQTYLPDH
CCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEECCCCC
PLLNLLIQHGYDEFANALLISRQEAEMPPFQFLAVIRAQGKTVNKVAQFLKATKDQIQAH
HHHHHHHHCCHHHHHHHHHHCCHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
PLKVLGPAPAPLPRKANQHRMQLLIKSPSRKVLKSSLTQLREWLTMNKLSNGIRWNVDVD
CEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
PMDLS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]