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The map label for this gene is nuoE [H]

Identifier: 148361098

GI number: 148361098

Start: 3315805

End: 3316308

Strand: Reverse

Name: nuoE [H]

Synonym: LPC_3071

Alternate gene names: 148361098

Gene position: 3316308-3315805 (Counterclockwise)

Preceding gene: 148361099

Following gene: 148361097

Centisome position: 92.73

GC content: 36.9

Gene sequence:

>504_bases
ATGTCTGCTAATTCAGCAAAAATATTAAATCAATTGATGCCGGCAAACCGTATGCGCGATATTGATCATTGGATATCAAA
ATATCCCAAAGATCAAAAACAATCCGCGGTCATGAGTGCTCTTCGAATAGTACAAGAAGAACATGGTCATTTAACAACGG
AATTAATGAATGCAGTAGCTGAATATCTTGATATGCCGCCCATCGCAGTTTATGAAGTAGCAAGTTTTTATACAATGTAT
GAACACAAGCCAGTGGGGCGACATTTAATTAATGTTTGTACCAATATTTCTTGCATGCTGAGGGATTCTGCTGGCGTTGT
TGAACATTTAGAAAAAAAATTGGGTGTTAATCTAGGTGGAACAACAGAAGATGGTCGATTTACTTTAAGATCAGTTGAAT
GCTTGGGTGCTTGTGTGAATGCGCCAATGATGCAAGTTGATAAAGATTATCACGAAAATTTGACTGCTGAATCTATAGAT
AAAGTATTGGAACAGTACCAATAG

Upstream 100 bases:

>100_bases
GCAAGTCAAGATATAGTGTTTGGAGAGATTGATCGTTAATTTAACGACATGCTTGCTTAAAGTGTATTAAAGAGATAATA
AACGATATAAAGGTTTCGAA

Downstream 100 bases:

>100_bases
AAAATAAAGGTGTTTGCCATGGTTGAACTAAATCAAGTTTGTTACCGAACATTTCATTTACCAGAGCCCTGGTCGCTATC
AACTTATGAAAGTATTGGTG

Product: NADH dehydrogenase I subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E 2; NDH-1 subunit E 2 [H]

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MSANSAKILNQLMPANRMRDIDHWISKYPKDQKQSAVMSALRIVQEEHGHLTTELMNAVAEYLDMPPIAVYEVASFYTMY
EHKPVGRHLINVCTNISCMLRDSAGVVEHLEKKLGVNLGGTTEDGRFTLRSVECLGACVNAPMMQVDKDYHENLTAESID
KVLEQYQ

Sequences:

>Translated_167_residues
MSANSAKILNQLMPANRMRDIDHWISKYPKDQKQSAVMSALRIVQEEHGHLTTELMNAVAEYLDMPPIAVYEVASFYTMY
EHKPVGRHLINVCTNISCMLRDSAGVVEHLEKKLGVNLGGTTEDGRFTLRSVECLGACVNAPMMQVDKDYHENLTAESID
KVLEQYQ
>Mature_166_residues
SANSAKILNQLMPANRMRDIDHWISKYPKDQKQSAVMSALRIVQEEHGHLTTELMNAVAEYLDMPPIAVYEVASFYTMYE
HKPVGRHLINVCTNISCMLRDSAGVVEHLEKKLGVNLGGTTEDGRFTLRSVECLGACVNAPMMQVDKDYHENLTAESIDK
VLEQYQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=156, Percent_Identity=48.0769230769231, Blast_Score=162, Evalue=1e-40,
Organism=Escherichia coli, GI1788621, Length=147, Percent_Identity=39.4557823129252, Blast_Score=113, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17561328, Length=140, Percent_Identity=45.7142857142857, Blast_Score=148, Evalue=1e-36,
Organism=Drosophila melanogaster, GI18859877, Length=149, Percent_Identity=42.9530201342282, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI21355353, Length=145, Percent_Identity=36.551724137931, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18844; Mature: 18713

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANSAKILNQLMPANRMRDIDHWISKYPKDQKQSAVMSALRIVQEEHGHLTTELMNAVA
CCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
EYLDMPPIAVYEVASFYTMYEHKPVGRHLINVCTNISCMLRDSAGVVEHLEKKLGVNLGG
HHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHCCCCCC
TTEDGRFTLRSVECLGACVNAPMMQVDKDYHENLTAESIDKVLEQYQ
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SANSAKILNQLMPANRMRDIDHWISKYPKDQKQSAVMSALRIVQEEHGHLTTELMNAVA
CCHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
EYLDMPPIAVYEVASFYTMYEHKPVGRHLINVCTNISCMLRDSAGVVEHLEKKLGVNLGG
HHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHCCCCCC
TTEDGRFTLRSVECLGACVNAPMMQVDKDYHENLTAESIDKVLEQYQ
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]