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The map label for this gene is mao2 [H]

Identifier: 148360696

GI number: 148360696

Start: 803518

End: 804753

Strand: Direct

Name: mao2 [H]

Synonym: LPC_2643

Alternate gene names: 148360696

Gene position: 803518-804753 (Clockwise)

Preceding gene: 148360697

Following gene: 148360695

Centisome position: 22.47

GC content: 41.99

Gene sequence:

>1236_bases
TTGGATAATGAAGTAATAAAGCAGCGGGCATTGGATTATCATGAGTTCCCTGTTCCAGGGAAACTCTCTGTTCATCTTAC
AAAATCGACTAATTCCCAAGACGATTTGTCTTTGGCCTATACACCTGGTGTTGCTGCTCCAGTTTTAGCCATAGCTGAAG
CGCCCGAAAATGCTTATCGTTTTACCAGCAAAGGCAATCTGGTTGCGGTGATGACCAATGGCACTGCAGTGTTAGGTTTG
GGAAATCTTGGGCCTTTAGCGAGTAAGCCTGTAATGGAAGGTAAAGCGGTATTATTTAAACGATTTGCCGATCTTGATGT
CTTTGATATAGAAATCGATGCAGAGGATCCTCAGTCTTTTATTGCTACTGCAAAGCGCATTGCCCCAACTTTTGGTGGCA
TAAATCTGGAAGACATCAAAGCGCCAGAGTGTTTTGAAATAGAACAAGCATTGATTCAGCAGTTAAATATTCCTGTATTT
CATGATGATCAACATGGTACTGCGATTGTAGTTGCAGCAGGTTTATTAAATGCGTTGGAACTACAGAACAAGAAATTAAG
TGATATTAAAATTGTTTGTATGGGCGCTGGGGCTGCTGGTATTGCTTCTATGCGACTTCTTGTGGCATTAGGAGCTCCCA
AAAGTAATATGCTGCTGCTAGATACCAAGGGAGTTATTCATGATGGCCGCGAGGATTTAAACCCCTATAAATTTGCTTTT
GCCAGAAATACTCTCTGTCGTACACTTGAAGATGCTTTGGTCGATGCCGATGTGTTTATTGGCGTTGCAAGGCCAGATTT
GCTGAATGCTCATTTATTAAGTTTGATGGCGCCCAATCCAGTGATTTTTGCTTTATCTAACCCTGATCCTGAAATCAAAC
CCGAATTGGCTCATAGTGTGCGTAAAGATCTGGTGATGGCAACTGGACGAAGTGATTATCCTAATCAAGTTAATAATGTA
TTATGTTTTCCTTATATTTTTCGTGGTGCGCTAGATGTTCGTGCCACTTGCATTAACCAGGCCATGCAAATTGCAGCTGT
TGAAGCCATTCGTCAATTGGTGCATGAGCCTGTTCCTCAGGTAGTGAAGGACAATTACCCAGGAGTTGTGAATTGGGATT
TTGGACCTGACTATATTATTCCAAAGCCTATTGATCCTCGCTTAAAAGAGAGGGTTCCTGCTGCTGTAGCGAAAGCTGCA
ATAGCCAGTGGCGCGAGTCAATTTAATAATTTATAA

Upstream 100 bases:

>100_bases
AGGCACAAATTTTTTGTGCCTTTTCTCTAAGAGAGGATTAAAAATTCAAATTTTTGATATGATGTTTTCACCGCGTGATC
TGAAATAGAGAGTATGCACT

Downstream 100 bases:

>100_bases
GGCAGTAAATTCACCTTATAGAAGTAAGCAGTTGAGGTCTTAATAGCGTTTAAACGAAGGATATCTTTGAAAGTTCTTTG
GAAACCATCAGTTGGTTTTC

Product: malate oxidoreductase

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MDNEVIKQRALDYHEFPVPGKLSVHLTKSTNSQDDLSLAYTPGVAAPVLAIAEAPENAYRFTSKGNLVAVMTNGTAVLGL
GNLGPLASKPVMEGKAVLFKRFADLDVFDIEIDAEDPQSFIATAKRIAPTFGGINLEDIKAPECFEIEQALIQQLNIPVF
HDDQHGTAIVVAAGLLNALELQNKKLSDIKIVCMGAGAAGIASMRLLVALGAPKSNMLLLDTKGVIHDGREDLNPYKFAF
ARNTLCRTLEDALVDADVFIGVARPDLLNAHLLSLMAPNPVIFALSNPDPEIKPELAHSVRKDLVMATGRSDYPNQVNNV
LCFPYIFRGALDVRATCINQAMQIAAVEAIRQLVHEPVPQVVKDNYPGVVNWDFGPDYIIPKPIDPRLKERVPAAVAKAA
IASGASQFNNL

Sequences:

>Translated_411_residues
MDNEVIKQRALDYHEFPVPGKLSVHLTKSTNSQDDLSLAYTPGVAAPVLAIAEAPENAYRFTSKGNLVAVMTNGTAVLGL
GNLGPLASKPVMEGKAVLFKRFADLDVFDIEIDAEDPQSFIATAKRIAPTFGGINLEDIKAPECFEIEQALIQQLNIPVF
HDDQHGTAIVVAAGLLNALELQNKKLSDIKIVCMGAGAAGIASMRLLVALGAPKSNMLLLDTKGVIHDGREDLNPYKFAF
ARNTLCRTLEDALVDADVFIGVARPDLLNAHLLSLMAPNPVIFALSNPDPEIKPELAHSVRKDLVMATGRSDYPNQVNNV
LCFPYIFRGALDVRATCINQAMQIAAVEAIRQLVHEPVPQVVKDNYPGVVNWDFGPDYIIPKPIDPRLKERVPAAVAKAA
IASGASQFNNL
>Mature_411_residues
MDNEVIKQRALDYHEFPVPGKLSVHLTKSTNSQDDLSLAYTPGVAAPVLAIAEAPENAYRFTSKGNLVAVMTNGTAVLGL
GNLGPLASKPVMEGKAVLFKRFADLDVFDIEIDAEDPQSFIATAKRIAPTFGGINLEDIKAPECFEIEQALIQQLNIPVF
HDDQHGTAIVVAAGLLNALELQNKKLSDIKIVCMGAGAAGIASMRLLVALGAPKSNMLLLDTKGVIHDGREDLNPYKFAF
ARNTLCRTLEDALVDADVFIGVARPDLLNAHLLSLMAPNPVIFALSNPDPEIKPELAHSVRKDLVMATGRSDYPNQVNNV
LCFPYIFRGALDVRATCINQAMQIAAVEAIRQLVHEPVPQVVKDNYPGVVNWDFGPDYIIPKPIDPRLKERVPAAVAKAA
IASGASQFNNL

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=347, Percent_Identity=27.6657060518732, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI62420882, Length=227, Percent_Identity=32.5991189427313, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI62420880, Length=227, Percent_Identity=32.5991189427313, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI239049447, Length=227, Percent_Identity=32.5991189427313, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788806, Length=408, Percent_Identity=58.3333333333333, Blast_Score=470, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6322823, Length=286, Percent_Identity=26.9230769230769, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24646386, Length=347, Percent_Identity=29.971181556196, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24646388, Length=347, Percent_Identity=29.971181556196, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 44399; Mature: 44399

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNEVIKQRALDYHEFPVPGKLSVHLTKSTNSQDDLSLAYTPGVAAPVLAIAEAPENAYR
CCHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHCCCCHHEE
FTSKGNLVAVMTNGTAVLGLGNLGPLASKPVMEGKAVLFKRFADLDVFDIEIDAEDPQSF
EECCCCEEEEEECCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCHHHH
IATAKRIAPTFGGINLEDIKAPECFEIEQALIQQLNIPVFHDDQHGTAIVVAAGLLNALE
HHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEHHHHHHHHH
LQNKKLSDIKIVCMGAGAAGIASMRLLVALGAPKSNMLLLDTKGVIHDGREDLNPYKFAF
HCCCCCCCEEEEEEECCHHHHHHHHHHEEECCCCCCEEEEECCCCCCCCHHCCCHHHHHH
ARNTLCRTLEDALVDADVFIGVARPDLLNAHLLSLMAPNPVIFALSNPDPEIKPELAHSV
HHHHHHHHHHHHHHCCHHEEEECCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHH
RKDLVMATGRSDYPNQVNNVLCFPYIFRGALDVRATCINQAMQIAAVEAIRQLVHEPVPQ
HHHHEEECCCCCCCHHHCCEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VVKDNYPGVVNWDFGPDYIIPKPIDPRLKERVPAAVAKAAIASGASQFNNL
HHHCCCCCEEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCHHHHCCC
>Mature Secondary Structure
MDNEVIKQRALDYHEFPVPGKLSVHLTKSTNSQDDLSLAYTPGVAAPVLAIAEAPENAYR
CCHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHCCCCHHEE
FTSKGNLVAVMTNGTAVLGLGNLGPLASKPVMEGKAVLFKRFADLDVFDIEIDAEDPQSF
EECCCCEEEEEECCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCHHHH
IATAKRIAPTFGGINLEDIKAPECFEIEQALIQQLNIPVFHDDQHGTAIVVAAGLLNALE
HHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEHHHHHHHHH
LQNKKLSDIKIVCMGAGAAGIASMRLLVALGAPKSNMLLLDTKGVIHDGREDLNPYKFAF
HCCCCCCCEEEEEEECCHHHHHHHHHHEEECCCCCCEEEEECCCCCCCCHHCCCHHHHHH
ARNTLCRTLEDALVDADVFIGVARPDLLNAHLLSLMAPNPVIFALSNPDPEIKPELAHSV
HHHHHHHHHHHHHHCCHHEEEECCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHH
RKDLVMATGRSDYPNQVNNVLCFPYIFRGALDVRATCINQAMQIAAVEAIRQLVHEPVPQ
HHHHEEECCCCCCCHHHCCEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VVKDNYPGVVNWDFGPDYIIPKPIDPRLKERVPAAVAKAAIASGASQFNNL
HHHCCCCCEEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]