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The map label for this gene is oprM [H]

Identifier: 148360684

GI number: 148360684

Start: 822516

End: 823955

Strand: Direct

Name: oprM [H]

Synonym: LPC_2630

Alternate gene names: 148360684

Gene position: 822516-823955 (Clockwise)

Preceding gene: 148360685

Following gene: 148360677

Centisome position: 23.0

GC content: 41.25

Gene sequence:

>1440_bases
ATGGACAGAAAATTAGTGCTACTCTTCAGTATTTCTTGTTTATTTGGCTGTACTGTCGGTCCTGATTATCAACGCCCGGA
CATTAAAATTCAGGAGAAATGGCCAACAAGCAAAGTAGAGAAAAAAATTTCCAGTAGTGCCCCTGTTCATTTAACCTGGT
GGAAGGCATTTCATGATCCATTGCTTGATCGATACATTAACGAGGCAGCTACTAATAACCTTGATATCAAAGCAGCAGAA
GCTCGTATCCGCCAAGCCCGCGCAATGCAACAAATTTCCCTTGCCAATTTTTATCCGCAGATCAATGGATCAGCAAGTGC
TAATTCCTTGCAAATCAGTAAGGGTACGTTGGAGGGGATTTTTGCAAACTCTCCAACATATACTCTAATTGATAGAAATC
AATGGATATATAATTTGGGTTTTGATGCACAATGGGAGTTGGATTTATTTGGTAAAACAAGAAGAAAAGTTGAAGCAAGC
AAAGCAAGCCTGGAAGAGGCTTATGAATCACGCCGTACCATTTTACTATCTACGATAGCCGAGATCGCAAGAAGTTATAT
AGAATTGAGAGGCGCGCAACAATTGTATGGTATTGCGCAAAAAAATGTGGAATTGCAAAGAAAAACGTTGGCTCTTGTCC
GCCAACGCTATAAAGCAGGTGTTAGCAGCGAAGTCGATGTTTCTTTAGCCGAAGGCCAGTTAAAAAATACAGAATCTGTT
TTACCCAATTATATTACCCAAATACAGGTAAATGCTTATCAAATTGCCGTTATGCTGGGTAAAGATCCGCAAACAATTCT
CAAAGATATTGCAAAAATTCAATCTTTACCCAAACCACCGCAAAAAGTACCTATGGGATTACGTTCAGATATTCTGCGCC
AGCGTCCAGACATAAGAGCAACAGAGAGGCAATTAGCGTCTGCCGTAGCTGAAATTGGTGTGGCAGTTGCTGACTTTTTC
CCGCAATTTAATCTTTCTGTTTCCAATCTTGCTTTTCAGAGCACTTCTGTTGATACGTTATTTCAATCCAGTAGTGGATT
TTGGCTCATGGGTCCTCAAGTCAGTTTACCCATATTTCATGGTGGCCAGATTAGAGCTAATGTTGAGAGTAAGAAGGCGG
TTGCTCAGCAAGCAGCAATACAATACAAAAAAATAATCCTCCAGGCATTGCAAGAGGCTCAAAGTGCTCTTGTCCGCTAT
AGCCAATCGCTCGATACAAACCATAGCTTGCGCGCTGCCATTATTGCTAACAAGCGAGCTGTGCAATTTGCCAAGAAACG
ATTTCAATACGGTGAGGATGATTATTTATCACTACTGCAATCAGAGGCTGCTCTTCTGAATGCCGAGACTCAAGAAGTCA
ATTCGGAGGTTTCTTCATTGACTGCTTTGATTAGTCTATATAAAGCGCTTGGCGGAGGTTGGGAAAACTTTGAGGCTTAA

Upstream 100 bases:

>100_bases
TAGAGGTTTTTATCACAACATGGCCACTTCTATTGGTAGGGGCTGTTACATTATTTATTGCTGGCTGGTTTTTTAAGCAG
CGTATGGAGTAAGAAACAGT

Downstream 100 bases:

>100_bases
AGTAGGCCTAAACATCTGTGCATGCTCTAAAATCAGGAACTTTATCAATAAATTAATGTAATAAGTTATGGATAAGGTAG
GTCATTATCTTGAATCGTTC

Product: multidrug efflux MFS outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MDRKLVLLFSISCLFGCTVGPDYQRPDIKIQEKWPTSKVEKKISSSAPVHLTWWKAFHDPLLDRYINEAATNNLDIKAAE
ARIRQARAMQQISLANFYPQINGSASANSLQISKGTLEGIFANSPTYTLIDRNQWIYNLGFDAQWELDLFGKTRRKVEAS
KASLEEAYESRRTILLSTIAEIARSYIELRGAQQLYGIAQKNVELQRKTLALVRQRYKAGVSSEVDVSLAEGQLKNTESV
LPNYITQIQVNAYQIAVMLGKDPQTILKDIAKIQSLPKPPQKVPMGLRSDILRQRPDIRATERQLASAVAEIGVAVADFF
PQFNLSVSNLAFQSTSVDTLFQSSSGFWLMGPQVSLPIFHGGQIRANVESKKAVAQQAAIQYKKIILQALQEAQSALVRY
SQSLDTNHSLRAAIIANKRAVQFAKKRFQYGEDDYLSLLQSEAALLNAETQEVNSEVSSLTALISLYKALGGGWENFEA

Sequences:

>Translated_479_residues
MDRKLVLLFSISCLFGCTVGPDYQRPDIKIQEKWPTSKVEKKISSSAPVHLTWWKAFHDPLLDRYINEAATNNLDIKAAE
ARIRQARAMQQISLANFYPQINGSASANSLQISKGTLEGIFANSPTYTLIDRNQWIYNLGFDAQWELDLFGKTRRKVEAS
KASLEEAYESRRTILLSTIAEIARSYIELRGAQQLYGIAQKNVELQRKTLALVRQRYKAGVSSEVDVSLAEGQLKNTESV
LPNYITQIQVNAYQIAVMLGKDPQTILKDIAKIQSLPKPPQKVPMGLRSDILRQRPDIRATERQLASAVAEIGVAVADFF
PQFNLSVSNLAFQSTSVDTLFQSSSGFWLMGPQVSLPIFHGGQIRANVESKKAVAQQAAIQYKKIILQALQEAQSALVRY
SQSLDTNHSLRAAIIANKRAVQFAKKRFQYGEDDYLSLLQSEAALLNAETQEVNSEVSSLTALISLYKALGGGWENFEA
>Mature_479_residues
MDRKLVLLFSISCLFGCTVGPDYQRPDIKIQEKWPTSKVEKKISSSAPVHLTWWKAFHDPLLDRYINEAATNNLDIKAAE
ARIRQARAMQQISLANFYPQINGSASANSLQISKGTLEGIFANSPTYTLIDRNQWIYNLGFDAQWELDLFGKTRRKVEAS
KASLEEAYESRRTILLSTIAEIARSYIELRGAQQLYGIAQKNVELQRKTLALVRQRYKAGVSSEVDVSLAEGQLKNTESV
LPNYITQIQVNAYQIAVMLGKDPQTILKDIAKIQSLPKPPQKVPMGLRSDILRQRPDIRATERQLASAVAEIGVAVADFF
PQFNLSVSNLAFQSTSVDTLFQSSSGFWLMGPQVSLPIFHGGQIRANVESKKAVAQQAAIQYKKIILQALQEAQSALVRY
SQSLDTNHSLRAAIIANKRAVQFAKKRFQYGEDDYLSLLQSEAALLNAETQEVNSEVSSLTALISLYKALGGGWENFEA

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=473, Percent_Identity=29.3868921775898, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI2367348, Length=475, Percent_Identity=21.8947368421053, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53439; Mature: 53439

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRKLVLLFSISCLFGCTVGPDYQRPDIKIQEKWPTSKVEKKISSSAPVHLTWWKAFHDP
CCCCEEEEEEHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHCCCCCEEEEHHHHHHHH
LLDRYINEAATNNLDIKAAEARIRQARAMQQISLANFYPQINGSASANSLQISKGTLEGI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEE
FANSPTYTLIDRNQWIYNLGFDAQWELDLFGKTRRKVEASKASLEEAYESRRTILLSTIA
ECCCCEEEEEECCCEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIARSYIELRGAQQLYGIAQKNVELQRKTLALVRQRYKAGVSSEVDVSLAEGQLKNTESV
HHHHHHHHHHCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHH
LPNYITQIQVNAYQIAVMLGKDPQTILKDIAKIQSLPKPPQKVPMGLRSDILRQRPDIRA
HHHHHHEEEEEEEEEEEEECCCHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHCCCCCHH
TERQLASAVAEIGVAVADFFPQFNLSVSNLAFQSTSVDTLFQSSSGFWLMGPQVSLPIFH
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCHHHHHCCCCCEEEECCCEECEEEE
GGQIRANVESKKAVAQQAAIQYKKIILQALQEAQSALVRYSQSLDTNHSLRAAIIANKRA
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHH
VQFAKKRFQYGEDDYLSLLQSEAALLNAETQEVNSEVSSLTALISLYKALGGGWENFEA
HHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDRKLVLLFSISCLFGCTVGPDYQRPDIKIQEKWPTSKVEKKISSSAPVHLTWWKAFHDP
CCCCEEEEEEHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHCCCCCEEEEHHHHHHHH
LLDRYINEAATNNLDIKAAEARIRQARAMQQISLANFYPQINGSASANSLQISKGTLEGI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEE
FANSPTYTLIDRNQWIYNLGFDAQWELDLFGKTRRKVEASKASLEEAYESRRTILLSTIA
ECCCCEEEEEECCCEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIARSYIELRGAQQLYGIAQKNVELQRKTLALVRQRYKAGVSSEVDVSLAEGQLKNTESV
HHHHHHHHHHCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHH
LPNYITQIQVNAYQIAVMLGKDPQTILKDIAKIQSLPKPPQKVPMGLRSDILRQRPDIRA
HHHHHHEEEEEEEEEEEEECCCHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHCCCCCHH
TERQLASAVAEIGVAVADFFPQFNLSVSNLAFQSTSVDTLFQSSSGFWLMGPQVSLPIFH
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCHHHHHCCCCCEEEECCCEECEEEE
GGQIRANVESKKAVAQQAAIQYKKIILQALQEAQSALVRYSQSLDTNHSLRAAIIANKRA
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHH
VQFAKKRFQYGEDDYLSLLQSEAALLNAETQEVNSEVSSLTALISLYKALGGGWENFEA
HHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]