Click here to switch to the map view.

The map label for this gene is hisF [H]

Identifier: 148360597

GI number: 148360597

Start: 929562

End: 930323

Strand: Direct

Name: hisF [H]

Synonym: LPC_2543

Alternate gene names: 148360597

Gene position: 929562-930323 (Clockwise)

Preceding gene: 148360598

Following gene: 148360596

Centisome position: 25.99

GC content: 41.99

Gene sequence:

>762_bases
ATGAGTAATGTCCGTCTAATTGCACGACTAGATATTAAAGGACCTAACCTAATCAAAGGAGTGCATCTGGAGGGATTAAG
AGTAGTAGGAAATCCCAATGAATATGCTATGGCATATTATGCACAAGGTGCAGATGAATTGATTTATATGGATACAGTTG
CATCTTTGTATGGGCGTAATAATTTGTCAGAGATTGTAAAGACTACTGCTGAAAATGTATTTATCCCAATTACTGTAGGT
GGCGGAATTCGCAGTGTTGATGATGCCAAGCAATTATTGCGTTGCGGTGCCGACAAAGTGGCCGTTAATACCGCTGCGAC
CAAAAATCCCGCTCTCATATCTGACATCGCCAGAAGATTTGGCTCGCAATGTATGGTCTTATCAATCGAGGCTAAGCGAG
ATATAAATGGACGATGGGAAGTCATGACAGACAATGGACGTGAACATACCGGAATGGATGTTGTTGATTGGGCTAGGAAT
GGCGAAGAATTTGGTGCAGGTGAAATTCTTCTGACCTCTATTGATCAGGAAGGTACGCGTAAGGGGTTTGATCTGGAATT
AGTGAAACAGGTGTCTTCCATGGTTTCTATCCCGGTGATTGCAAGTGGGGGTATGGGTAAGCTGGAAGATTTAACTGAAG
TGGTTTGTGAGTCTAAGGCTGATGCAGTCGCTATGGCGGATGTTTTGCATTATAAAAGAATAGGTTTACCTGAAATCAGA
AAGTATGCTTTAGATAATGGCATTCATGTGAGAGTCTTATGA

Upstream 100 bases:

>100_bases
CTCACTTGTGGAAAAAAGAGGGAGAACAGTGGAAACTTAGACATCAAGTAACAAATCTTGAAAAAACTAAAGCTGAATAT
CTGGATTTGGAGACTGTTTG

Downstream 100 bases:

>100_bases
GTTCAGTTTCAATTGTAGATTATGGGGTAGGCAATTTATTAAGTGTGGCTCGAGCTTTTCAATATTTTGATGCTTCTGTC
AATTTAGTGAGCACACCCGA

Product: imidazole glycerol phosphate synthase cyclase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MSNVRLIARLDIKGPNLIKGVHLEGLRVVGNPNEYAMAYYAQGADELIYMDTVASLYGRNNLSEIVKTTAENVFIPITVG
GGIRSVDDAKQLLRCGADKVAVNTAATKNPALISDIARRFGSQCMVLSIEAKRDINGRWEVMTDNGREHTGMDVVDWARN
GEEFGAGEILLTSIDQEGTRKGFDLELVKQVSSMVSIPVIASGGMGKLEDLTEVVCESKADAVAMADVLHYKRIGLPEIR
KYALDNGIHVRVL

Sequences:

>Translated_253_residues
MSNVRLIARLDIKGPNLIKGVHLEGLRVVGNPNEYAMAYYAQGADELIYMDTVASLYGRNNLSEIVKTTAENVFIPITVG
GGIRSVDDAKQLLRCGADKVAVNTAATKNPALISDIARRFGSQCMVLSIEAKRDINGRWEVMTDNGREHTGMDVVDWARN
GEEFGAGEILLTSIDQEGTRKGFDLELVKQVSSMVSIPVIASGGMGKLEDLTEVVCESKADAVAMADVLHYKRIGLPEIR
KYALDNGIHVRVL
>Mature_252_residues
SNVRLIARLDIKGPNLIKGVHLEGLRVVGNPNEYAMAYYAQGADELIYMDTVASLYGRNNLSEIVKTTAENVFIPITVGG
GIRSVDDAKQLLRCGADKVAVNTAATKNPALISDIARRFGSQCMVLSIEAKRDINGRWEVMTDNGREHTGMDVVDWARNG
EEFGAGEILLTSIDQEGTRKGFDLELVKQVSSMVSIPVIASGGMGKLEDLTEVVCESKADAVAMADVLHYKRIGLPEIRK
YALDNGIHVRVL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=253, Percent_Identity=35.9683794466403, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI87082028, Length=183, Percent_Identity=27.3224043715847, Blast_Score=81, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6319725, Length=279, Percent_Identity=32.258064516129, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27550; Mature: 27419

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVRLIARLDIKGPNLIKGVHLEGLRVVGNPNEYAMAYYAQGADELIYMDTVASLYGRN
CCCEEEEEEEECCCCCEEEEEEECCEEEECCCCCEEEEEEECCCCCEEHHHHHHHHHCCC
NLSEIVKTTAENVFIPITVGGGIRSVDDAKQLLRCGADKVAVNTAATKNPALISDIARRF
CHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHH
GSQCMVLSIEAKRDINGRWEVMTDNGREHTGMDVVDWARNGEEFGAGEILLTSIDQEGTR
CCCEEEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCC
KGFDLELVKQVSSMVSIPVIASGGMGKLEDLTEVVCESKADAVAMADVLHYKRIGLPEIR
CCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHH
KYALDNGIHVRVL
HHHHCCCEEEEEC
>Mature Secondary Structure 
SNVRLIARLDIKGPNLIKGVHLEGLRVVGNPNEYAMAYYAQGADELIYMDTVASLYGRN
CCEEEEEEEECCCCCEEEEEEECCEEEECCCCCEEEEEEECCCCCEEHHHHHHHHHCCC
NLSEIVKTTAENVFIPITVGGGIRSVDDAKQLLRCGADKVAVNTAATKNPALISDIARRF
CHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHH
GSQCMVLSIEAKRDINGRWEVMTDNGREHTGMDVVDWARNGEEFGAGEILLTSIDQEGTR
CCCEEEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCC
KGFDLELVKQVSSMVSIPVIASGGMGKLEDLTEVVCESKADAVAMADVLHYKRIGLPEIR
CCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHH
KYALDNGIHVRVL
HHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11043980 [H]