Click here to switch to the map view.

The map label for this gene is hisH [H]

Identifier: 148360596

GI number: 148360596

Start: 930320

End: 930961

Strand: Direct

Name: hisH [H]

Synonym: LPC_2542

Alternate gene names: 148360596

Gene position: 930320-930961 (Clockwise)

Preceding gene: 148360597

Following gene: 148360595

Centisome position: 26.01

GC content: 38.79

Gene sequence:

>642_bases
ATGAGTTCAGTTTCAATTGTAGATTATGGGGTAGGCAATTTATTAAGTGTGGCTCGAGCTTTTCAATATTTTGATGCTTC
TGTCAATTTAGTGAGCACACCCGAGGAAATTATGAGTGCCGATCGGTTGGTTTTGCCAGGAGTAGGCGCTTTTGAAGATG
GGATGAAAGGATTAACTACATTAAATTTTATAGAACCCATAAAGCAATTTGCGCAGAGTGGAAAACCTTTTCTGGGAATA
TGTCTTGGCATGCAGATGATGCTTTCCAAAAGTACTGAATTTGGACAACATGAAGGTCTGGGTCTAATTGCTGGCGAGGT
TGTTAGCGTCCCTTCACATGGAGTTGATGGTCAATTGCATAAAATACCTCATATAGGCTGGAATGAACTGGTTTCTACCT
CAGAAGGCGAGGATTGGTGTCATACTATCCTGAAAAATATACCATTAAATTCTTCAGTATATTTTGTTCATTCTTTTATG
GCCATGCCTAGTAATCCTAAAAAGCGTCTGGCTGATACTTTATATGATGGTCAGGCTATTAGCGCAGTAATAAAAGATGA
AAATATGTACGGATGCCAATTTCATCCTGAAAAAAGCGGGGAAGTAGGTTTAAGCATCATTCAACAGTTTTTGCAGATTT
AG

Upstream 100 bases:

>100_bases
CTGATGCAGTCGCTATGGCGGATGTTTTGCATTATAAAAGAATAGGTTTACCTGAAATCAGAAAGTATGCTTTAGATAAT
GGCATTCATGTGAGAGTCTT

Downstream 100 bases:

>100_bases
GGTGAATTAAAAATGAGAATATTGGCAGTAATCCCGGCAAGGGCTGGCTCAAAGAGGCTGCCCGGTAAAAATACCAGATT
ACTTGCCGGAAAACCATTAA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit 1; IGP synthase subunit hisH 1; ImGP synthase subunit hisH 1; IGPS subunit hisH 1 [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MSSVSIVDYGVGNLLSVARAFQYFDASVNLVSTPEEIMSADRLVLPGVGAFEDGMKGLTTLNFIEPIKQFAQSGKPFLGI
CLGMQMMLSKSTEFGQHEGLGLIAGEVVSVPSHGVDGQLHKIPHIGWNELVSTSEGEDWCHTILKNIPLNSSVYFVHSFM
AMPSNPKKRLADTLYDGQAISAVIKDENMYGCQFHPEKSGEVGLSIIQQFLQI

Sequences:

>Translated_213_residues
MSSVSIVDYGVGNLLSVARAFQYFDASVNLVSTPEEIMSADRLVLPGVGAFEDGMKGLTTLNFIEPIKQFAQSGKPFLGI
CLGMQMMLSKSTEFGQHEGLGLIAGEVVSVPSHGVDGQLHKIPHIGWNELVSTSEGEDWCHTILKNIPLNSSVYFVHSFM
AMPSNPKKRLADTLYDGQAISAVIKDENMYGCQFHPEKSGEVGLSIIQQFLQI
>Mature_212_residues
SSVSIVDYGVGNLLSVARAFQYFDASVNLVSTPEEIMSADRLVLPGVGAFEDGMKGLTTLNFIEPIKQFAQSGKPFLGIC
LGMQMMLSKSTEFGQHEGLGLIAGEVVSVPSHGVDGQLHKIPHIGWNELVSTSEGEDWCHTILKNIPLNSSVYFVHSFMA
MPSNPKKRLADTLYDGQAISAVIKDENMYGCQFHPEKSGEVGLSIIQQFLQI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=211, Percent_Identity=33.6492890995261, Blast_Score=113, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6319725, Length=222, Percent_Identity=31.5315315315315, Blast_Score=122, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 23196; Mature: 23065

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVSIVDYGVGNLLSVARAFQYFDASVNLVSTPEEIMSADRLVLPGVGAFEDGMKGLTT
CCCCEEECCCHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCCEECCCCCCHHHHHHHHHH
LNFIEPIKQFAQSGKPFLGICLGMQMMLSKSTEFGQHEGLGLIAGEVVSVPSHGVDGQLH
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHEEECCCCCCCCCHH
KIPHIGWNELVSTSEGEDWCHTILKNIPLNSSVYFVHSFMAMPSNPKKRLADTLYDGQAI
CCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHH
SAVIKDENMYGCQFHPEKSGEVGLSIIQQFLQI
HHEEECCCEECCEECCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SSVSIVDYGVGNLLSVARAFQYFDASVNLVSTPEEIMSADRLVLPGVGAFEDGMKGLTT
CCCEEECCCHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCCEECCCCCCHHHHHHHHHH
LNFIEPIKQFAQSGKPFLGICLGMQMMLSKSTEFGQHEGLGLIAGEVVSVPSHGVDGQLH
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHEEECCCCCCCCCHH
KIPHIGWNELVSTSEGEDWCHTILKNIPLNSSVYFVHSFMAMPSNPKKRLADTLYDGQAI
CCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHH
SAVIKDENMYGCQFHPEKSGEVGLSIIQQFLQI
HHEEECCCEECCEECCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA