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The map label for this gene is rmlB [H]

Identifier: 148360588

GI number: 148360588

Start: 937445

End: 938512

Strand: Direct

Name: rmlB [H]

Synonym: LPC_2534

Alternate gene names: 148360588

Gene position: 937445-938512 (Clockwise)

Preceding gene: 148360589

Following gene: 148360587

Centisome position: 26.21

GC content: 34.74

Gene sequence:

>1068_bases
ATGTTATCGACCATTTTACAAAAAAGTAAGAATCACACTATGAAAAATCAGATGAACAATCTTTTGGTAACTGGAGCTGC
GGGCTTTATAGGAAGTAATTTTGTTAAATTTATGAACGATAAATACCCGGAGATCAAAATAATCAGTTTGGATAAATTAA
CCTATGCAGGAAATAAAGCCAATTTGAGTGAAATGGCAGAATGTAAAAATCACCTTTTTGTGCAAGGAGACATATTGGAC
AAAAGTCTGGTGTTATCCTTACTAAGGGAATATGAAATAGATACGCTTGTCCATTTTGCAGCCGAATCCCATGTGGATAA
TTCCATTGATAATCCGCAAATATTTCTTGAAACCAATGTAATTGGAACTTTTACTCTGTTAGAGGCGGCAAGGATTTATT
GGTTGAATGAAAGGCAATGGGATAAATCAAAATGTCGGTTTCATCATGTATCCACCGATGAAGTGTATGGTTCATTAGAA
AGAGAAGAGCCGGCGTTCACTGAAAAAAATTCATATCAACCTAACTCTCCTTATTCAGCGTCCAAAGCAAGTTCTGATCA
TATAGTCAGAGCTTATTATCATACCTATGGATTACCTGTTACCACTTCGAATTGTTCGAATAATTACGGGCCTAATCAAC
ATAAAGAGAAGTTGATTCCTAAGGTAGTATTCGCTTGTGTCAATCAGCTGCCCATCACCGTTTATGGCAACGGATCAAAT
ATTCGCGATTGGTTGTATGTCATGGATCATTGCGAAGCAATTGATACCATTATTCAAAAAGGAGCATTAGGGGAAGTTTA
TAATATAGGTGGTAATAACGAACTGGATAATTTAAGTTTGATAAAAATGATTTGTCAGATGATGGATGATTTAAAACCTA
TGGAAAAACCATATCATTCATTGATTACTTTTGTTGAAGATAGAAAAGGTCATGACAAACGATACGCCATAGACAATAGT
AAAATTCAAAAGGAATTGGGTTGGGTTCCACAAGGTGATTTTGTTCATAAATTATCAAATACAGTTCATCATTACTTAAC
ACGGTATGAGCGCGAAATTAATATTTAA

Upstream 100 bases:

>100_bases
CCTACTAGGGCAACAAGGCCTAAAAATTCGGTACTCGATACAACAAAGATAGAAACAATATTGGGTATAAAAAGCTGTAG
TTGGAAAAATGATTTGCCAG

Downstream 100 bases:

>100_bases
TAAATTGAAAATAATCAGGTAACATAGACTTTATTTTGATGATTAGAAATAGCATGAAGTCTCACACAGAATTATTGTCA
TGGAATTTGCTTCAAAAAGA

Product: dTDP-glucose 4,6-dehydratase RmlB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MLSTILQKSKNHTMKNQMNNLLVTGAAGFIGSNFVKFMNDKYPEIKIISLDKLTYAGNKANLSEMAECKNHLFVQGDILD
KSLVLSLLREYEIDTLVHFAAESHVDNSIDNPQIFLETNVIGTFTLLEAARIYWLNERQWDKSKCRFHHVSTDEVYGSLE
REEPAFTEKNSYQPNSPYSASKASSDHIVRAYYHTYGLPVTTSNCSNNYGPNQHKEKLIPKVVFACVNQLPITVYGNGSN
IRDWLYVMDHCEAIDTIIQKGALGEVYNIGGNNELDNLSLIKMICQMMDDLKPMEKPYHSLITFVEDRKGHDKRYAIDNS
KIQKELGWVPQGDFVHKLSNTVHHYLTRYEREINI

Sequences:

>Translated_355_residues
MLSTILQKSKNHTMKNQMNNLLVTGAAGFIGSNFVKFMNDKYPEIKIISLDKLTYAGNKANLSEMAECKNHLFVQGDILD
KSLVLSLLREYEIDTLVHFAAESHVDNSIDNPQIFLETNVIGTFTLLEAARIYWLNERQWDKSKCRFHHVSTDEVYGSLE
REEPAFTEKNSYQPNSPYSASKASSDHIVRAYYHTYGLPVTTSNCSNNYGPNQHKEKLIPKVVFACVNQLPITVYGNGSN
IRDWLYVMDHCEAIDTIIQKGALGEVYNIGGNNELDNLSLIKMICQMMDDLKPMEKPYHSLITFVEDRKGHDKRYAIDNS
KIQKELGWVPQGDFVHKLSNTVHHYLTRYEREINI
>Mature_355_residues
MLSTILQKSKNHTMKNQMNNLLVTGAAGFIGSNFVKFMNDKYPEIKIISLDKLTYAGNKANLSEMAECKNHLFVQGDILD
KSLVLSLLREYEIDTLVHFAAESHVDNSIDNPQIFLETNVIGTFTLLEAARIYWLNERQWDKSKCRFHHVSTDEVYGSLE
REEPAFTEKNSYQPNSPYSASKASSDHIVRAYYHTYGLPVTTSNCSNNYGPNQHKEKLIPKVVFACVNQLPITVYGNGSN
IRDWLYVMDHCEAIDTIIQKGALGEVYNIGGNNELDNLSLIKMICQMMDDLKPMEKPYHSLITFVEDRKGHDKRYAIDNS
KIQKELGWVPQGDFVHKLSNTVHHYLTRYEREINI

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=336, Percent_Identity=36.3095238095238, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI42516563, Length=340, Percent_Identity=24.4117647058824, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI4504031, Length=278, Percent_Identity=24.8201438848921, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI48994969, Length=335, Percent_Identity=50.4477611940299, Blast_Score=347, Evalue=5e-97,
Organism=Escherichia coli, GI1788353, Length=335, Percent_Identity=48.0597014925373, Blast_Score=337, Evalue=5e-94,
Organism=Escherichia coli, GI1786974, Length=340, Percent_Identity=27.0588235294118, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788366, Length=247, Percent_Identity=28.7449392712551, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17568069, Length=328, Percent_Identity=36.890243902439, Blast_Score=212, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI115532424, Length=337, Percent_Identity=32.9376854599407, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17539532, Length=337, Percent_Identity=24.9258160237389, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17539422, Length=188, Percent_Identity=30.8510638297872, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI133901788, Length=188, Percent_Identity=30.8510638297872, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17539424, Length=188, Percent_Identity=30.8510638297872, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI133901786, Length=188, Percent_Identity=30.8510638297872, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI133901790, Length=188, Percent_Identity=30.8510638297872, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17507723, Length=189, Percent_Identity=30.1587301587302, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71982038, Length=348, Percent_Identity=25.8620689655172, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=179, Percent_Identity=29.608938547486, Blast_Score=63, Evalue=7e-11,
Organism=Drosophila melanogaster, GI19923002, Length=343, Percent_Identity=27.9883381924198, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356223, Length=339, Percent_Identity=23.3038348082596, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24158427, Length=249, Percent_Identity=25.3012048192771, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40666; Mature: 40666

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSTILQKSKNHTMKNQMNNLLVTGAAGFIGSNFVKFMNDKYPEIKIISLDKLTYAGNKA
CCHHHHHHHHCCHHHHHHCCEEEECCCHHHHHHHHHHHCCCCCCEEEEEEECEEECCCCC
NLSEMAECKNHLFVQGDILDKSLVLSLLREYEIDTLVHFAAESHVDNSIDNPQIFLETNV
CHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
IGTFTLLEAARIYWLNERQWDKSKCRFHHVSTDEVYGSLEREEPAFTEKNSYQPNSPYSA
HHHHHHHHHHHHEEECCCCCCCHHCEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
SKASSDHIVRAYYHTYGLPVTTSNCSNNYGPNQHKEKLIPKVVFACVNQLPITVYGNGSN
CCCCCCCEEEEEHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCC
IRDWLYVMDHCEAIDTIIQKGALGEVYNIGGNNELDNLSLIKMICQMMDDLKPMEKPYHS
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
LITFVEDRKGHDKRYAIDNSKIQKELGWVPQGDFVHKLSNTVHHYLTRYEREINI
HHHHHHHCCCCCCEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLSTILQKSKNHTMKNQMNNLLVTGAAGFIGSNFVKFMNDKYPEIKIISLDKLTYAGNKA
CCHHHHHHHHCCHHHHHHCCEEEECCCHHHHHHHHHHHCCCCCCEEEEEEECEEECCCCC
NLSEMAECKNHLFVQGDILDKSLVLSLLREYEIDTLVHFAAESHVDNSIDNPQIFLETNV
CHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
IGTFTLLEAARIYWLNERQWDKSKCRFHHVSTDEVYGSLEREEPAFTEKNSYQPNSPYSA
HHHHHHHHHHHHEEECCCCCCCHHCEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
SKASSDHIVRAYYHTYGLPVTTSNCSNNYGPNQHKEKLIPKVVFACVNQLPITVYGNGSN
CCCCCCCEEEEEHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCC
IRDWLYVMDHCEAIDTIIQKGALGEVYNIGGNNELDNLSLIKMICQMMDDLKPMEKPYHS
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
LITFVEDRKGHDKRYAIDNSKIQKELGWVPQGDFVHKLSNTVHHYLTRYEREINI
HHHHHHHCCCCCCEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]