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The map label for this gene is prfC [H]

Identifier: 148360480

GI number: 148360480

Start: 1053223

End: 1054803

Strand: Direct

Name: prfC [H]

Synonym: LPC_2420

Alternate gene names: 148360480

Gene position: 1053223-1054803 (Clockwise)

Preceding gene: 148360483

Following gene: 148360473

Centisome position: 29.45

GC content: 39.28

Gene sequence:

>1581_bases
ATGTCTGATTTTTATCAAGACTTTAATAAAAGGCGAACTTTTGCCATTATTTCTCACCCTGATGCAGGAAAGACCACTGT
AACTGAAAAGTTATTGCTTTTTGGAGGAGCTATTCAGTTGGCAGGTACGGTCAAAGGTCGTAAAGCAGACAGACATGCCA
CTTCTGATTGGATGGAAATGGAAAAAGAGAGAGGAATATCGATTACCACGTCTGTTATGCAGTTTATACATAATCAACAC
GTCATCAATTTGTTAGATACACCAGGCCATGAAGACTTTTCTGAAGATACCTATCGTACTCTCACTGCTGTAGATTCTGC
CTTAATGGTAATTGACGTGGCCAAGGGAGTAGAGGAGCGAACGGTAAAATTGATGGAAGTTTGCCGTTTACGTGATACAC
CGATCATGACTTTTATCAATAAATTGGACAGAGAAGGGCGAGAACCTATTGATTTGTTAGATGAAGTAGAGTCTGTTTTG
GGCATTCAATGTGCGCCAATTACCTGGCCAGTTGGAATGGGTAAACGCTTCAAGGGAATTTATCACCGCTATCAAGATAT
TATTTATTTATATCAGCAAGGCAGTAATGCAAAAAAAGTGGAAGCTATGCAGATTAAAGGATTGGATAATCCTCAATTAG
ATGAGTTGATAGGTGACAGTGCAGACGAACTCAGAGAAGAAATTGAATTAGTCAAAGGAGCGTCCCACGGGTTTAACTTG
GAAGATTATCTTGCAGGAAAAATGACCCCGGTATACTTTGGTTCAGCAATTAATAATTTTGGTATTAAAGAATTATTGGA
CGATTTTGTCGAGTATGCGCCAGGACCTCAACCTCGAGCAACTCAGGAGAGAGTTGTTTCTCCGAATGAAGAAACATTTT
CCGGTTTTGTTTTTAAAATTCAAGCCAATATGGATCCCAAACACAGAGACCGAATTGCCTTTCTGAGGGTTTGTTCTGGC
GCTTACAAGAAGGGAATGAAATTAAGTCATTTACGCATAGGAAAAGAGGTGCAAATTTCCAATGCCTTAACTTTTATGGC
AGGCGATAGATCACACACAGAACTTGCTTTAGCTGGTGATATTATTGGTTTGCATAATCATGGTACTATTCGTATTGGTG
ATACTTTTACACAAGGTGAGCAATTAAAGTTTACTGGAATTCCTAATTTTGCTCCTGAATTGTTTAGGCTTGTCAGGTTG
AGGGACCCATTAAAGAGTAAAGCCTTGCTTAAAGGATTAATCGAATTATCTGAAGAGGGCGCCACCCAGGTTTTTAGGCC
ATTAAACAGCAATCAGTTAATATTGGGAGCTGTTGGGATATTACAATTTGATGTGGTAGCGCATAGACTGAAACATGAAT
ATAAAGTGGATTGTATTTATGAATCAGTCAATGTGACTTGCGCTCGATGGGTTTATTCTGAAGATGATAAAGCAATGAGT
GAATTTCGTACCAAGGCTTATGATTATTTGGCTTTGGATGGAGGAGATATGCTTATGTACCTGGCGCCTACCAAAGTGAA
TTTGACAATGGCAGAAGAACGTTATCCCAAGATTAAGTTCTGTGCTACTCGAGAACATTGA

Upstream 100 bases:

>100_bases
GATAAACACCAGCGAGATTTTAATTTATAACGAAAAAATTGTTAGTTTTGTTCTTTTCCTTTACAATTCAAGGCATTTAA
CCACTTAATCCGAGATTCCT

Downstream 100 bases:

>100_bases
TAAACAGGATTTGAATGTTCGCACCCCATTACTGTTCAGTGTTGGGGTGCGGTTAGCTCAATTCTTTATGCAGTACAAGT
TGCTTTGAGAGTATGTGGTC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSDFYQDFNKRRTFAIISHPDAGKTTVTEKLLLFGGAIQLAGTVKGRKADRHATSDWMEMEKERGISITTSVMQFIHNQH
VINLLDTPGHEDFSEDTYRTLTAVDSALMVIDVAKGVEERTVKLMEVCRLRDTPIMTFINKLDREGREPIDLLDEVESVL
GIQCAPITWPVGMGKRFKGIYHRYQDIIYLYQQGSNAKKVEAMQIKGLDNPQLDELIGDSADELREEIELVKGASHGFNL
EDYLAGKMTPVYFGSAINNFGIKELLDDFVEYAPGPQPRATQERVVSPNEETFSGFVFKIQANMDPKHRDRIAFLRVCSG
AYKKGMKLSHLRIGKEVQISNALTFMAGDRSHTELALAGDIIGLHNHGTIRIGDTFTQGEQLKFTGIPNFAPELFRLVRL
RDPLKSKALLKGLIELSEEGATQVFRPLNSNQLILGAVGILQFDVVAHRLKHEYKVDCIYESVNVTCARWVYSEDDKAMS
EFRTKAYDYLALDGGDMLMYLAPTKVNLTMAEERYPKIKFCATREH

Sequences:

>Translated_526_residues
MSDFYQDFNKRRTFAIISHPDAGKTTVTEKLLLFGGAIQLAGTVKGRKADRHATSDWMEMEKERGISITTSVMQFIHNQH
VINLLDTPGHEDFSEDTYRTLTAVDSALMVIDVAKGVEERTVKLMEVCRLRDTPIMTFINKLDREGREPIDLLDEVESVL
GIQCAPITWPVGMGKRFKGIYHRYQDIIYLYQQGSNAKKVEAMQIKGLDNPQLDELIGDSADELREEIELVKGASHGFNL
EDYLAGKMTPVYFGSAINNFGIKELLDDFVEYAPGPQPRATQERVVSPNEETFSGFVFKIQANMDPKHRDRIAFLRVCSG
AYKKGMKLSHLRIGKEVQISNALTFMAGDRSHTELALAGDIIGLHNHGTIRIGDTFTQGEQLKFTGIPNFAPELFRLVRL
RDPLKSKALLKGLIELSEEGATQVFRPLNSNQLILGAVGILQFDVVAHRLKHEYKVDCIYESVNVTCARWVYSEDDKAMS
EFRTKAYDYLALDGGDMLMYLAPTKVNLTMAEERYPKIKFCATREH
>Mature_525_residues
SDFYQDFNKRRTFAIISHPDAGKTTVTEKLLLFGGAIQLAGTVKGRKADRHATSDWMEMEKERGISITTSVMQFIHNQHV
INLLDTPGHEDFSEDTYRTLTAVDSALMVIDVAKGVEERTVKLMEVCRLRDTPIMTFINKLDREGREPIDLLDEVESVLG
IQCAPITWPVGMGKRFKGIYHRYQDIIYLYQQGSNAKKVEAMQIKGLDNPQLDELIGDSADELREEIELVKGASHGFNLE
DYLAGKMTPVYFGSAINNFGIKELLDDFVEYAPGPQPRATQERVVSPNEETFSGFVFKIQANMDPKHRDRIAFLRVCSGA
YKKGMKLSHLRIGKEVQISNALTFMAGDRSHTELALAGDIIGLHNHGTIRIGDTFTQGEQLKFTGIPNFAPELFRLVRLR
DPLKSKALLKGLIELSEEGATQVFRPLNSNQLILGAVGILQFDVVAHRLKHEYKVDCIYESVNVTCARWVYSEDDKAMSE
FRTKAYDYLALDGGDMLMYLAPTKVNLTMAEERYPKIKFCATREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=488, Percent_Identity=27.6639344262295, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI19923640, Length=498, Percent_Identity=25.5020080321285, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI25306283, Length=391, Percent_Identity=27.3657289002558, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI25306287, Length=291, Percent_Identity=29.2096219931272, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI157426893, Length=168, Percent_Identity=30.952380952381, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=29.9270072992701, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1790835, Length=527, Percent_Identity=67.1726755218216, Blast_Score=742, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=497, Percent_Identity=27.364185110664, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=32.0512820512821, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI48994988, Length=147, Percent_Identity=36.734693877551, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=503, Percent_Identity=27.8330019880716, Blast_Score=158, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17556745, Length=179, Percent_Identity=35.7541899441341, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17557151, Length=146, Percent_Identity=32.8767123287671, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17506493, Length=162, Percent_Identity=30.2469135802469, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=30.8270676691729, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=30.8270676691729, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=480, Percent_Identity=27.7083333333333, Blast_Score=172, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6322359, Length=542, Percent_Identity=24.3542435424354, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324707, Length=150, Percent_Identity=32.6666666666667, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320593, Length=150, Percent_Identity=32.6666666666667, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=27.536231884058, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582462, Length=476, Percent_Identity=28.1512605042017, Blast_Score=162, Evalue=8e-40,
Organism=Drosophila melanogaster, GI221458488, Length=502, Percent_Identity=27.6892430278884, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI78706572, Length=155, Percent_Identity=31.6129032258064, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585711, Length=154, Percent_Identity=32.4675324675325, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24585713, Length=154, Percent_Identity=32.4675324675325, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=32.4675324675325, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59404; Mature: 59273

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFYQDFNKRRTFAIISHPDAGKTTVTEKLLLFGGAIQLAGTVKGRKADRHATSDWMEM
CCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHH
EKERGISITTSVMQFIHNQHVINLLDTPGHEDFSEDTYRTLTAVDSALMVIDVAKGVEER
HHHCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
TVKLMEVCRLRDTPIMTFINKLDREGREPIDLLDEVESVLGIQCAPITWPVGMGKRFKGI
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCEEECCCCCHHHHHH
YHRYQDIIYLYQQGSNAKKVEAMQIKGLDNPQLDELIGDSADELREEIELVKGASHGFNL
HHHHHHHEEEEECCCCCCEEEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCH
EDYLAGKMTPVYFGSAINNFGIKELLDDFVEYAPGPQPRATQERVVSPNEETFSGFVFKI
HHHHCCCCCCEEECCHHCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCEEEEEE
QANMDPKHRDRIAFLRVCSGAYKKGMKLSHLRIGKEVQISNALTFMAGDRSHTELALAGD
ECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCEEEEEECCCCCCEEEEECC
IIGLHNHGTIRIGDTFTQGEQLKFTGIPNFAPELFRLVRLRDPLKSKALLKGLIELSEEG
EEEECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
ATQVFRPLNSNQLILGAVGILQFDVVAHRLKHEYKVDCIYESVNVTCARWVYSEDDKAMS
HHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCHHHHH
EFRTKAYDYLALDGGDMLMYLAPTKVNLTMAEERYPKIKFCATREH
HHHHHHHEEEEECCCCEEEEEECCEEEEEEHHCCCCCEEEEECCCC
>Mature Secondary Structure 
SDFYQDFNKRRTFAIISHPDAGKTTVTEKLLLFGGAIQLAGTVKGRKADRHATSDWMEM
CHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHH
EKERGISITTSVMQFIHNQHVINLLDTPGHEDFSEDTYRTLTAVDSALMVIDVAKGVEER
HHHCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
TVKLMEVCRLRDTPIMTFINKLDREGREPIDLLDEVESVLGIQCAPITWPVGMGKRFKGI
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCEEECCCCCHHHHHH
YHRYQDIIYLYQQGSNAKKVEAMQIKGLDNPQLDELIGDSADELREEIELVKGASHGFNL
HHHHHHHEEEEECCCCCCEEEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCH
EDYLAGKMTPVYFGSAINNFGIKELLDDFVEYAPGPQPRATQERVVSPNEETFSGFVFKI
HHHHCCCCCCEEECCHHCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCEEEEEE
QANMDPKHRDRIAFLRVCSGAYKKGMKLSHLRIGKEVQISNALTFMAGDRSHTELALAGD
ECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCEEEEEECCCCCCEEEEECC
IIGLHNHGTIRIGDTFTQGEQLKFTGIPNFAPELFRLVRLRDPLKSKALLKGLIELSEEG
EEEECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
ATQVFRPLNSNQLILGAVGILQFDVVAHRLKHEYKVDCIYESVNVTCARWVYSEDDKAMS
HHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCHHHHH
EFRTKAYDYLALDGGDMLMYLAPTKVNLTMAEERYPKIKFCATREH
HHHHHHHEEEEECCCCEEEEEECCEEEEEEHHCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA