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The map label for this gene is ald [H]

Identifier: 148360428

GI number: 148360428

Start: 1104187

End: 1105308

Strand: Direct

Name: ald [H]

Synonym: LPC_2367

Alternate gene names: 148360428

Gene position: 1104187-1105308 (Clockwise)

Preceding gene: 148360429

Following gene: 148360425

Centisome position: 30.87

GC content: 44.39

Gene sequence:

>1122_bases
ATGTTAGTAGGGGTACCAAAAGAAATCAAATCGCACGAAAACAGAGTAGGACTTGTTCCCTCGAGTGTTAGAGAAATTAT
TAGGGCAGGAAGTGATGTATTGGTAGAGAAGGGAGCTGGGCTTGGTATCGGCATATCGGATGATGATTACAGAAATGCCG
GGGCTGAAGTGGTTGATACGGCTGATGATGTATTTGCCAATGCCGAATTAATAGTCAAAGTAAAAGAGCCCCAGCCTATT
GAATGTAAACGGTTGCGTGAAGGGCAAACATTATTCACTTATTTACATTTGGCTCCTGATCCTCATCAAGCACGTTTATT
AAAAGAATCAGGGGTTACAGCGATAGCTTATGAAACTGTAACACAGGATGATGGCGGGTTACCTTTACTTACTCCCATGT
CCCAAGTGGCTGGTCGTATGGCAATCCAGGCGGGTGCACATTGCTTGGAGATGGCTCAAGGGGGTAGCGGTATATTACTA
GGTGGTGTACCTGGTGTTGCTGCAGCCAATGTCGTGGTCATTGGCGGTGGAGTCGTAGGAACAAATGCTGTTCGCATGGC
GATGGGGATGGAAGCTCGTGTTATTGTGCTAGATCGTTCACTGCAACGTTTAAATGAATTGGATTTTCAGTTTGGTTCTA
AACTCAATACAGTTTATTCAACTGCAGATTCCATAGAAAAATACGTATCCAGCGCTGATTTAGTGATTGGTGCTGTATTG
GTACCAGGTGCTGCAGCCCCCAAATTAGTGACTCGCTCTATGCTGAGCTCAATGCGTCCTGGCTCCGTTTTAGTCGATGT
TGCTATTGATCAAGGAGGGTGTTTTGAGACGAGTCGTGCAACAACCCATCAAGAACCTACTTACGTGGTCGATAACGTAG
TGCATTATTGTGTGGCCAATATGCCTGGTGCTGTACCCAGAACATCAACATTTGCTTTAAATAATGCTACTTTACCTTTC
ATTTTAAGTATTGTTACCAAAGGGGTTAAGCTGGCTTTATTGAGTGACCCGCATTTACTTAATGGCCTGAATGTTCACCA
GGGTAAAATCACTTATGAGGCTGTTGCTCGTGATTTAGGATATGAATACACGCCAGCTGCAGCTGCTTTATCAGGACGAT
AA

Upstream 100 bases:

>100_bases
CACCTATTTTTCAAATCGCGACTTATGGGTTAGTAGGTGATCTGTTTGAATTAGTTCCACAATTAATAGAACAATTAAAG
AATCGATAAGAGGTAACAAT

Downstream 100 bases:

>100_bases
TTATTAGATTAAAACCCGTATTACACAAATTGAATACGGGTTTTGTGCTTCATCAGAATATAAATCTTCAAATTAAAACA
GACTTTCTTCCACTGGTGCT

Product: alanine dehydrogenase

Products: NA

Alternate protein names: Stage V sporulation protein N [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MLVGVPKEIKSHENRVGLVPSSVREIIRAGSDVLVEKGAGLGIGISDDDYRNAGAEVVDTADDVFANAELIVKVKEPQPI
ECKRLREGQTLFTYLHLAPDPHQARLLKESGVTAIAYETVTQDDGGLPLLTPMSQVAGRMAIQAGAHCLEMAQGGSGILL
GGVPGVAAANVVVIGGGVVGTNAVRMAMGMEARVIVLDRSLQRLNELDFQFGSKLNTVYSTADSIEKYVSSADLVIGAVL
VPGAAAPKLVTRSMLSSMRPGSVLVDVAIDQGGCFETSRATTHQEPTYVVDNVVHYCVANMPGAVPRTSTFALNNATLPF
ILSIVTKGVKLALLSDPHLLNGLNVHQGKITYEAVARDLGYEYTPAAAALSGR

Sequences:

>Translated_373_residues
MLVGVPKEIKSHENRVGLVPSSVREIIRAGSDVLVEKGAGLGIGISDDDYRNAGAEVVDTADDVFANAELIVKVKEPQPI
ECKRLREGQTLFTYLHLAPDPHQARLLKESGVTAIAYETVTQDDGGLPLLTPMSQVAGRMAIQAGAHCLEMAQGGSGILL
GGVPGVAAANVVVIGGGVVGTNAVRMAMGMEARVIVLDRSLQRLNELDFQFGSKLNTVYSTADSIEKYVSSADLVIGAVL
VPGAAAPKLVTRSMLSSMRPGSVLVDVAIDQGGCFETSRATTHQEPTYVVDNVVHYCVANMPGAVPRTSTFALNNATLPF
ILSIVTKGVKLALLSDPHLLNGLNVHQGKITYEAVARDLGYEYTPAAAALSGR
>Mature_373_residues
MLVGVPKEIKSHENRVGLVPSSVREIIRAGSDVLVEKGAGLGIGISDDDYRNAGAEVVDTADDVFANAELIVKVKEPQPI
ECKRLREGQTLFTYLHLAPDPHQARLLKESGVTAIAYETVTQDDGGLPLLTPMSQVAGRMAIQAGAHCLEMAQGGSGILL
GGVPGVAAANVVVIGGGVVGTNAVRMAMGMEARVIVLDRSLQRLNELDFQFGSKLNTVYSTADSIEKYVSSADLVIGAVL
VPGAAAPKLVTRSMLSSMRPGSVLVDVAIDQGGCFETSRATTHQEPTYVVDNVVHYCVANMPGAVPRTSTFALNNATLPF
ILSIVTKGVKLALLSDPHLLNGLNVHQGKITYEAVARDLGYEYTPAAAALSGR

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=346, Percent_Identity=30.3468208092486, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI122939153, Length=346, Percent_Identity=30.3468208092486, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1787887, Length=383, Percent_Identity=30.2872062663185, Blast_Score=126, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17550456, Length=346, Percent_Identity=30.9248554913295, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 39239; Mature: 39239

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVGVPKEIKSHENRVGLVPSSVREIIRAGSDVLVEKGAGLGIGISDDDYRNAGAEVVDT
CCCCCCHHHHCCCCEECCCHHHHHHHHHCCCCEEEECCCCCEECCCCCCHHCCCCHHHCC
ADDVFANAELIVKVKEPQPIECKRLREGQTLFTYLHLAPDPHQARLLKESGVTAIAYETV
HHHHHCCCEEEEEECCCCCCHHHHHCCCCEEEEEEEECCCHHHHHHHHHCCCEEEEEEEE
TQDDGGLPLLTPMSQVAGRMAIQAGAHCLEMAQGGSGILLGGVPGVAAANVVVIGGGVVG
ECCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCEEC
TNAVRMAMGMEARVIVLDRSLQRLNELDFQFGSKLNTVYSTADSIEKYVSSADLVIGAVL
CHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHEEEHEE
VPGAAAPKLVTRSMLSSMRPGSVLVDVAIDQGGCFETSRATTHQEPTYVVDNVVHYCVAN
CCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
MPGAVPRTSTFALNNATLPFILSIVTKGVKLALLSDPHLLNGLNVHQGKITYEAVARDLG
CCCCCCCCCEEEECCCCHHHHHHHHHCCEEEEEECCCHHHCCCCCCCCEEHHHHHHHHCC
YEYTPAAAALSGR
CCCCCHHHHCCCC
>Mature Secondary Structure
MLVGVPKEIKSHENRVGLVPSSVREIIRAGSDVLVEKGAGLGIGISDDDYRNAGAEVVDT
CCCCCCHHHHCCCCEECCCHHHHHHHHHCCCCEEEECCCCCEECCCCCCHHCCCCHHHCC
ADDVFANAELIVKVKEPQPIECKRLREGQTLFTYLHLAPDPHQARLLKESGVTAIAYETV
HHHHHCCCEEEEEECCCCCCHHHHHCCCCEEEEEEEECCCHHHHHHHHHCCCEEEEEEEE
TQDDGGLPLLTPMSQVAGRMAIQAGAHCLEMAQGGSGILLGGVPGVAAANVVVIGGGVVG
ECCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCEEC
TNAVRMAMGMEARVIVLDRSLQRLNELDFQFGSKLNTVYSTADSIEKYVSSADLVIGAVL
CHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHEEEHEE
VPGAAAPKLVTRSMLSSMRPGSVLVDVAIDQGGCFETSRATTHQEPTYVVDNVVHYCVAN
CCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
MPGAVPRTSTFALNNATLPFILSIVTKGVKLALLSDPHLLNGLNVHQGKITYEAVARDLG
CCCCCCCCCEEEECCCCHHHHHHHHHCCEEEEEECCCHHHCCCCCCCCEEHHHHHHHHCC
YEYTPAAAALSGR
CCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226620; 9384377 [H]