Click here to switch to the map view.

The map label for this gene is nitA [C]

Identifier: 148360398

GI number: 148360398

Start: 1139509

End: 1140315

Strand: Direct

Name: nitA [C]

Synonym: LPC_2337

Alternate gene names: 148360398

Gene position: 1139509-1140315 (Clockwise)

Preceding gene: 148360400

Following gene: 148360397

Centisome position: 31.86

GC content: 40.27

Gene sequence:

>807_bases
ATGAATCGAGTAGCGCTTGTGCAAATGGTATCATCTGCCAAAATAGCAGATAATTTGCAACTCACTGAACAATATTTAAT
TCAAGCCAGGGAACAGGAAGCCAGTTTGGTTGTGCTACCTGAGAATTTTGCTTTTATGGGAATGAATGAACGAGAGAAGT
TGCACATTGCTGAATATTATGGTCAAGGATCTATACAACAAAGAATAAGTGAGTTAGCGAGAGATTTGCGGGTATGGATA
ATAGCAGGAACAATCCCGTTGAAAAGTATGGGTTCAAAGGTAAGAGCAAGTTGTATAGTTTATGATGATAAAGGCCTAAA
TGTTGCTCGCTATGATAAAATTCATTTATTTGATGTCATAGTTTCCGAGCAGGAAAAACACCAGGAATCGTTAACTATAG
AGGCAGGAAAAGATATAGCGCTAGTGGATACGCCAGTGGGGAAAATTGGGTTAACCATCTGTTATGACTTGCGTTTTCCG
GAATTGTACCAATATTTAACCCAAAGAGGCGCACAATTACTTTCAGTTCCGTCTGCTTTTACTGCGGTAACTGGAGCTGC
GCATTGGGAGGTCTTACTCAGAGCAAGAGCAATCGAAAATTTATGTTATGTCTTAGCTCCTAATCAGGGTGGGACTCATG
AAAACGGGCGTCATACTTATGGGCATAGTATGGTGGTGGAACCATGGGGTAAAGTGTTGGCTCAAAAAGAAGAAGGGCAG
GGAGTAATTCTGGCTGATATTGATTTAGAGAGATTGTCACAGTTGAGAAGACAATTTCCGTGTGTTGAACATCATGTTTT
AATGTAA

Upstream 100 bases:

>100_bases
TTTATTATTAAGGCGAAAAGGTTTTTGTGTCAATTTTGTTTTGGGCTTATATGGAACGATATAGTAAGATGAAAAATATG
AATGTCATTTAGGAAAAAGG

Downstream 100 bases:

>100_bases
AAGGTATATAAATGACAAAATCTCTTGCGATAGCAAAAAATATTTTATTGACACCAGCTTCCCTGGATGAAGCCGGCATA
GAAAAATTATTCAAGACCAT

Product: nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MNRVALVQMVSSAKIADNLQLTEQYLIQAREQEASLVVLPENFAFMGMNEREKLHIAEYYGQGSIQQRISELARDLRVWI
IAGTIPLKSMGSKVRASCIVYDDKGLNVARYDKIHLFDVIVSEQEKHQESLTIEAGKDIALVDTPVGKIGLTICYDLRFP
ELYQYLTQRGAQLLSVPSAFTAVTGAAHWEVLLRARAIENLCYVLAPNQGGTHENGRHTYGHSMVVEPWGKVLAQKEEGQ
GVILADIDLERLSQLRRQFPCVEHHVLM

Sequences:

>Translated_268_residues
MNRVALVQMVSSAKIADNLQLTEQYLIQAREQEASLVVLPENFAFMGMNEREKLHIAEYYGQGSIQQRISELARDLRVWI
IAGTIPLKSMGSKVRASCIVYDDKGLNVARYDKIHLFDVIVSEQEKHQESLTIEAGKDIALVDTPVGKIGLTICYDLRFP
ELYQYLTQRGAQLLSVPSAFTAVTGAAHWEVLLRARAIENLCYVLAPNQGGTHENGRHTYGHSMVVEPWGKVLAQKEEGQ
GVILADIDLERLSQLRRQFPCVEHHVLM
>Mature_268_residues
MNRVALVQMVSSAKIADNLQLTEQYLIQAREQEASLVVLPENFAFMGMNEREKLHIAEYYGQGSIQQRISELARDLRVWI
IAGTIPLKSMGSKVRASCIVYDDKGLNVARYDKIHLFDVIVSEQEKHQESLTIEAGKDIALVDTPVGKIGLTICYDLRFP
ELYQYLTQRGAQLLSVPSAFTAVTGAAHWEVLLRARAIENLCYVLAPNQGGTHENGRHTYGHSMVVEPWGKVLAQKEEGQ
GVILADIDLERLSQLRRQFPCVEHHVLM

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI5031947, Length=266, Percent_Identity=42.8571428571429, Blast_Score=211, Evalue=5e-55,
Organism=Homo sapiens, GI297632350, Length=266, Percent_Identity=42.8571428571429, Blast_Score=211, Evalue=6e-55,
Organism=Homo sapiens, GI297632348, Length=266, Percent_Identity=42.8571428571429, Blast_Score=211, Evalue=7e-55,
Organism=Homo sapiens, GI9910460, Length=264, Percent_Identity=37.8787878787879, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI297632346, Length=192, Percent_Identity=36.4583333333333, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1786412, Length=227, Percent_Identity=30.8370044052863, Blast_Score=84, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17556280, Length=264, Percent_Identity=42.4242424242424, Blast_Score=214, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6322335, Length=292, Percent_Identity=35.2739726027397, Blast_Score=179, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6323383, Length=268, Percent_Identity=35.4477611940299, Blast_Score=166, Evalue=4e-42,
Organism=Drosophila melanogaster, GI17933642, Length=263, Percent_Identity=40.3041825095057, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI21355835, Length=259, Percent_Identity=34.7490347490347, Blast_Score=163, Evalue=9e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 30143; Mature: 30143

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRVALVQMVSSAKIADNLQLTEQYLIQAREQEASLVVLPENFAFMGMNEREKLHIAEYY
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCEEEEEHHH
GQGSIQQRISELARDLRVWIIAGTIPLKSMGSKVRASCIVYDDKGLNVARYDKIHLFDVI
CCCHHHHHHHHHHHHEEEEEEECCCCHHHCCCCCEEEEEEEECCCCCEEEECCEEEEHHH
VSEQEKHQESLTIEAGKDIALVDTPVGKIGLTICYDLRFPELYQYLTQRGAQLLSVPSAF
HCCHHHHHHCEEEECCCCEEEEECCCCCEEEEEEEECCCHHHHHHHHHCCHHEECCCCHH
TAVTGAAHWEVLLRARAIENLCYVLAPNQGGTHENGRHTYGHSMVVEPWGKVLAQKEEGQ
HHHHCHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCEECCCCEEECCHHHHHHCCCCCC
GVILADIDLERLSQLRRQFPCVEHHVLM
EEEEEECCHHHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure
MNRVALVQMVSSAKIADNLQLTEQYLIQAREQEASLVVLPENFAFMGMNEREKLHIAEYY
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCEEEEEHHH
GQGSIQQRISELARDLRVWIIAGTIPLKSMGSKVRASCIVYDDKGLNVARYDKIHLFDVI
CCCHHHHHHHHHHHHEEEEEEECCCCHHHCCCCCEEEEEEEECCCCCEEEECCEEEEHHH
VSEQEKHQESLTIEAGKDIALVDTPVGKIGLTICYDLRFPELYQYLTQRGAQLLSVPSAF
HCCHHHHHHCEEEECCCCEEEEECCCCCEEEEEEEECCCHHHHHHHHHCCHHEECCCCHH
TAVTGAAHWEVLLRARAIENLCYVLAPNQGGTHENGRHTYGHSMVVEPWGKVLAQKEEGQ
HHHHCHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCEECCCCEEECCHHHHHHCCCCCC
GVILADIDLERLSQLRRQFPCVEHHVLM
EEEEEECCHHHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]