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The map label for this gene is cadA [H]

Identifier: 148359914

GI number: 148359914

Start: 2788704

End: 2790137

Strand: Reverse

Name: cadA [H]

Synonym: LPC_1843

Alternate gene names: 148359914

Gene position: 2790137-2788704 (Counterclockwise)

Preceding gene: 148359915

Following gene: 148359913

Centisome position: 78.01

GC content: 38.63

Gene sequence:

>1434_bases
ATGGGCTGCTCGACATGCTCTTCACATAAGGAGCCAATGCCAAAGTTGTGGCATAATCCCAAAGTCATTACAGCAACTAT
AGCTGGTTTATTATTAACTATTGGTTTTGTAGGCTCATATTTGGCATTACCAATGATTATCGCTAATGGGTTTTACATAG
GCGCAGTATTAATTGGCGGATATTATTTCGCACGCGAAGCAATAGACGAATTTATCAAAGAATATGAAATCGGTATTGAA
TTCTTGATGTCTCTTGCAGCCATTGTTGCAGGTATCATGGGGCAATGGGCGGAAGCAGCAACATTGGTATTTTTGTATTC
TATTTCTGAAGCTGCGGAAGGTTATTCCGGTGAAAGAGCGCGGCATGCTATCCGAGCATTGATGGATCTTGCCCCAAAAA
CAGCATTAATCCGGCGAAATAATACTGAATTTCGAATACCCATAGAAGAAATTCATATTGGAGATGAATTCATTGTTCTT
CCTGGTGAAAGTCTTGCTACAGATGGAGAAGTCATTTCGGGACATTCTAGTGTTAATCAAGCACCTATTACGGGTGAGTC
AATTCCAGTAGAAAAAACTATAGGGAGTAAAGTTTTTTCTGCAACTATAAACGGTGAAGGAACTCTTACTGTTCGTGCAA
CCAAAAAATTTGCTGATAATACATTATCTCGTATCATATTTCTTGTCGAAAAAGCACAAGACAAAAAGGGGCGTAGTCAA
CGTTTTATAGAACGTTTTGGCAACCTATATAGTCCATTTATCCTTTTGGTGGGAATACTCATAGCTACTTTACCACCTCT
ATTTGGGCAACCTTGGCAAGAGTGGGTGATTCGTGCCACAGTATTTATTGTTGCTGCAGCACCATGTGCCCTAGTGATTT
CTATACCCATTACACTTGTAGCTGCTCTTGGCACAGCAAGTCGAAATGGAATATTACTAAAAGGTGGATTATATTTGGAG
CAACTAGTGAAGATAAAAGTTATTGCACTAGATAAAACAGGAACATTAACACAAGGTAAACCGAGGGTTACCGATATCAT
TCCATTGAATAATCATACTCGGAACCAAATTTTAAATGCAGCAGCAACTCTTGAATCGCGTTCCCAGCATCCAATGGCCC
AAGCCATTTTGCAACAAGCCAATGAAGAAAATATTCAATATAATCCTGTTGAACAGTTTAAGTCATTAACTGGTTCAGGT
GTACTTGGTTTTATCAATGGGAAAAAATTCTACATAGGCAATCCAAAATTATTTTATGATATGGGCCTATTGATAGAAAC
ATTAACACAGGAAATAAATAAATTACAGGCCGCTGGAAAGACAGTCGTATTATTAGGAACTGATAAGGAAATCCTCGGTT
TAATTGCAATTGCGGATCCTTCTAAGACCAACAGCAAGGCAGATGGTGTCAAACTTAAAATCGATGGGTATTGA

Upstream 100 bases:

>100_bases
GTTCAATATCTATGATAGAGATGGTTCTCTAACAACCGTTACTTTTACGCGACATATAGCATCAGTGTTTAAAAAAATTA
CGAATAAGGAGTGTAAATAA

Downstream 100 bases:

>100_bases
ACGTGTGGTTATGCTCACAGGAGATAATTTGATAACTGCCTCCTCAATTGGCGAACAAGCGGGTGTTGATGAGGTATTTT
CTGATTTATCACCAGAAGAT

Product: cadmium efflux ATPase

Products: NA

Alternate protein names: Cadmium efflux ATPase [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MGCSTCSSHKEPMPKLWHNPKVITATIAGLLLTIGFVGSYLALPMIIANGFYIGAVLIGGYYFAREAIDEFIKEYEIGIE
FLMSLAAIVAGIMGQWAEAATLVFLYSISEAAEGYSGERARHAIRALMDLAPKTALIRRNNTEFRIPIEEIHIGDEFIVL
PGESLATDGEVISGHSSVNQAPITGESIPVEKTIGSKVFSATINGEGTLTVRATKKFADNTLSRIIFLVEKAQDKKGRSQ
RFIERFGNLYSPFILLVGILIATLPPLFGQPWQEWVIRATVFIVAAAPCALVISIPITLVAALGTASRNGILLKGGLYLE
QLVKIKVIALDKTGTLTQGKPRVTDIIPLNNHTRNQILNAAATLESRSQHPMAQAILQQANEENIQYNPVEQFKSLTGSG
VLGFINGKKFYIGNPKLFYDMGLLIETLTQEINKLQAAGKTVVLLGTDKEILGLIAIADPSKTNSKADGVKLKIDGY

Sequences:

>Translated_477_residues
MGCSTCSSHKEPMPKLWHNPKVITATIAGLLLTIGFVGSYLALPMIIANGFYIGAVLIGGYYFAREAIDEFIKEYEIGIE
FLMSLAAIVAGIMGQWAEAATLVFLYSISEAAEGYSGERARHAIRALMDLAPKTALIRRNNTEFRIPIEEIHIGDEFIVL
PGESLATDGEVISGHSSVNQAPITGESIPVEKTIGSKVFSATINGEGTLTVRATKKFADNTLSRIIFLVEKAQDKKGRSQ
RFIERFGNLYSPFILLVGILIATLPPLFGQPWQEWVIRATVFIVAAAPCALVISIPITLVAALGTASRNGILLKGGLYLE
QLVKIKVIALDKTGTLTQGKPRVTDIIPLNNHTRNQILNAAATLESRSQHPMAQAILQQANEENIQYNPVEQFKSLTGSG
VLGFINGKKFYIGNPKLFYDMGLLIETLTQEINKLQAAGKTVVLLGTDKEILGLIAIADPSKTNSKADGVKLKIDGY
>Mature_476_residues
GCSTCSSHKEPMPKLWHNPKVITATIAGLLLTIGFVGSYLALPMIIANGFYIGAVLIGGYYFAREAIDEFIKEYEIGIEF
LMSLAAIVAGIMGQWAEAATLVFLYSISEAAEGYSGERARHAIRALMDLAPKTALIRRNNTEFRIPIEEIHIGDEFIVLP
GESLATDGEVISGHSSVNQAPITGESIPVEKTIGSKVFSATINGEGTLTVRATKKFADNTLSRIIFLVEKAQDKKGRSQR
FIERFGNLYSPFILLVGILIATLPPLFGQPWQEWVIRATVFIVAAAPCALVISIPITLVAALGTASRNGILLKGGLYLEQ
LVKIKVIALDKTGTLTQGKPRVTDIIPLNNHTRNQILNAAATLESRSQHPMAQAILQQANEENIQYNPVEQFKSLTGSGV
LGFINGKKFYIGNPKLFYDMGLLIETLTQEINKLQAAGKTVVLLGTDKEILGLIAIADPSKTNSKADGVKLKIDGY

Specific function: This electroneutral antiporter ejects one cadmium ion while accumulating two protons by an energy-dependent efflux mechanism [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI115529486, Length=401, Percent_Identity=30.1745635910224, Blast_Score=162, Evalue=8e-40,
Organism=Homo sapiens, GI55743071, Length=495, Percent_Identity=26.4646464646465, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI55743073, Length=368, Percent_Identity=28.2608695652174, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI4502271, Length=341, Percent_Identity=23.7536656891496, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI22748667, Length=268, Percent_Identity=24.6268656716418, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI21361181, Length=363, Percent_Identity=23.4159779614325, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI237681109, Length=336, Percent_Identity=23.8095238095238, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI237681111, Length=336, Percent_Identity=23.8095238095238, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI153946397, Length=247, Percent_Identity=24.6963562753036, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI1789879, Length=419, Percent_Identity=36.0381861575179, Blast_Score=248, Evalue=7e-67,
Organism=Escherichia coli, GI1786691, Length=382, Percent_Identity=30.8900523560209, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1786914, Length=359, Percent_Identity=25.0696378830084, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556548, Length=411, Percent_Identity=28.4671532846715, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6319772, Length=321, Percent_Identity=31.1526479750779, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320475, Length=396, Percent_Identity=28.2828282828283, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321430, Length=303, Percent_Identity=25.7425742574257, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325221, Length=309, Percent_Identity=27.1844660194175, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI221329854, Length=369, Percent_Identity=28.7262872628726, Blast_Score=133, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR001366
- InterPro:   IPR006404
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00403 HMA; PF00702 Hydrolase [H]

EC number: =3.6.3.3 [H]

Molecular weight: Translated: 51742; Mature: 51611

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00154 ATPASE_E1_E2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGCSTCSSHKEPMPKLWHNPKVITATIAGLLLTIGFVGSYLALPMIIANGFYIGAVLIGG
CCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
YYFAREAIDEFIKEYEIGIEFLMSLAAIVAGIMGQWAEAATLVFLYSISEAAEGYSGERA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHCCCCCHHH
RHAIRALMDLAPKTALIRRNNTEFRIPIEEIHIGDEFIVLPGESLATDGEVISGHSSVNQ
HHHHHHHHHHCCCCEEEECCCCEEEEEHHHEECCCEEEEECCCCCCCCCCEEECCCCCCC
APITGESIPVEKTIGSKVFSATINGEGTLTVRATKKFADNTLSRIIFLVEKAQDKKGRSQ
CCCCCCCCCCHHHHCCCEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHH
RFIERFGNLYSPFILLVGILIATLPPLFGQPWQEWVIRATVFIVAAAPCALVISIPITLV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
AALGTASRNGILLKGGLYLEQLVKIKVIALDKTGTLTQGKPRVTDIIPLNNHTRNQILNA
HHHCCCCCCCEEEECCHHHHHHHEEEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHH
AATLESRSQHPMAQAILQQANEENIQYNPVEQFKSLTGSGVLGFINGKKFYIGNPKLFYD
HHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCEEEEECCCEEEECCCCHHHH
MGLLIETLTQEINKLQAAGKTVVLLGTDKEILGLIAIADPSKTNSKADGVKLKIDGY
HHHHHHHHHHHHHHHHHCCCEEEEEECCHHHEEEEEEECCCCCCCCCCCEEEEECCC
>Mature Secondary Structure 
GCSTCSSHKEPMPKLWHNPKVITATIAGLLLTIGFVGSYLALPMIIANGFYIGAVLIGG
CCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
YYFAREAIDEFIKEYEIGIEFLMSLAAIVAGIMGQWAEAATLVFLYSISEAAEGYSGERA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHCCCCCHHH
RHAIRALMDLAPKTALIRRNNTEFRIPIEEIHIGDEFIVLPGESLATDGEVISGHSSVNQ
HHHHHHHHHHCCCCEEEECCCCEEEEEHHHEECCCEEEEECCCCCCCCCCEEECCCCCCC
APITGESIPVEKTIGSKVFSATINGEGTLTVRATKKFADNTLSRIIFLVEKAQDKKGRSQ
CCCCCCCCCCHHHHCCCEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHH
RFIERFGNLYSPFILLVGILIATLPPLFGQPWQEWVIRATVFIVAAAPCALVISIPITLV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
AALGTASRNGILLKGGLYLEQLVKIKVIALDKTGTLTQGKPRVTDIIPLNNHTRNQILNA
HHHCCCCCCCEEEECCHHHHHHHEEEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHH
AATLESRSQHPMAQAILQQANEENIQYNPVEQFKSLTGSGVLGFINGKKFYIGNPKLFYD
HHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCEEEEECCCEEEECCCCHHHH
MGLLIETLTQEINKLQAAGKTVVLLGTDKEILGLIAIADPSKTNSKADGVKLKIDGY
HHHHHHHHHHHHHHHHHCCCEEEEEECCHHHEEEEEEECCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1321115 [H]