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The map label for this gene is tphB [H]

Identifier: 148359662

GI number: 148359662

Start: 2459405

End: 2460118

Strand: Direct

Name: tphB [H]

Synonym: LPC_1580

Alternate gene names: 148359662

Gene position: 2459405-2460118 (Clockwise)

Preceding gene: 148359660

Following gene: 148359663

Centisome position: 68.77

GC content: 40.34

Gene sequence:

>714_bases
ATGGAAAATAAGAAGAAAAGAGTCGTTGTTGCAACCGCCGGAACTTTTCTGGAATGGGCTGAGTTTACTTATTACGCCTA
TATCGCAAATATTATGTCGGGATCGTTTTTTCCAAAATTAGGAAATCATCTTGGTTTGATAGCAACCTTTTCTGTTTTCG
CGCTCAGTTACTTTTTCCGTCCTTTAGGAGCCCTCTTTTTTGGATATCTTGGAGACAAGATGGGGCGTCGGGTTGCCCTG
CAATCTTCTATTATGCTGATGGGGTTATCTTCACTATGCATTGGTTGTCTTCCTGCTTACGATAGTATTGGTATCTTGGC
TCCAATTCTTTTACTGATATTCCGTTGTGTTCAAGGCTTTGCCGTGTCTGGTGAATTTAATGGCAGCGCGATCTATCTGA
TTGAACATGATTCACAAAAACCATGCCAAGCCGGTAGCTGGACTGGATTTGCTTCCGCATTGGGTATGATGTTTGGCGGA
TTGATGTCTACTTTAATTTATCTACCTCACATGCCTGAGTGGGCATGGCGTATCCCTTTCCTGTTAGGAACTTTATCCTG
CTTTTGTGCGATGTATTTTCGTAAAAATCTGAGTGAATCACCGCCCTACCTTACGCTAAGTAACGAAAAATCATCCAATC
CACTGAAAATTTTATTTGAAAATTATAAAAAACAATTAATTAGGGCAGTGTTATTAATAGCTGCATTAACGTGA

Upstream 100 bases:

>100_bases
AACTCTATGACTCTGTTATATCATCCAATTACATAGAATAATGAGATCAATTTAAAGTAAAATCTCTCTACATCACTTTA
ATATTCTATTGATTTATTGC

Downstream 100 bases:

>100_bases
ATTCGATATAACTGTCTAAAAAACAAACTTAAGAATGGTAACATATTGAAAAACCAGACCATTTGTGATCTTATTCGTTT
TTTCGAAGAACAAAAATAAG

Product: proline/glycine betaine transporter-like protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MENKKKRVVVATAGTFLEWAEFTYYAYIANIMSGSFFPKLGNHLGLIATFSVFALSYFFRPLGALFFGYLGDKMGRRVAL
QSSIMLMGLSSLCIGCLPAYDSIGILAPILLLIFRCVQGFAVSGEFNGSAIYLIEHDSQKPCQAGSWTGFASALGMMFGG
LMSTLIYLPHMPEWAWRIPFLLGTLSCFCAMYFRKNLSESPPYLTLSNEKSSNPLKILFENYKKQLIRAVLLIAALT

Sequences:

>Translated_237_residues
MENKKKRVVVATAGTFLEWAEFTYYAYIANIMSGSFFPKLGNHLGLIATFSVFALSYFFRPLGALFFGYLGDKMGRRVAL
QSSIMLMGLSSLCIGCLPAYDSIGILAPILLLIFRCVQGFAVSGEFNGSAIYLIEHDSQKPCQAGSWTGFASALGMMFGG
LMSTLIYLPHMPEWAWRIPFLLGTLSCFCAMYFRKNLSESPPYLTLSNEKSSNPLKILFENYKKQLIRAVLLIAALT
>Mature_237_residues
MENKKKRVVVATAGTFLEWAEFTYYAYIANIMSGSFFPKLGNHLGLIATFSVFALSYFFRPLGALFFGYLGDKMGRRVAL
QSSIMLMGLSSLCIGCLPAYDSIGILAPILLLIFRCVQGFAVSGEFNGSAIYLIEHDSQKPCQAGSWTGFASALGMMFGG
LMSTLIYLPHMPEWAWRIPFLLGTLSCFCAMYFRKNLSESPPYLTLSNEKSSNPLKILFENYKKQLIRAVLLIAALT

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=204, Percent_Identity=38.2352941176471, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1788292, Length=224, Percent_Identity=30.3571428571429, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1789941, Length=206, Percent_Identity=33.9805825242718, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1788942, Length=234, Percent_Identity=32.4786324786325, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI87082231, Length=229, Percent_Identity=27.0742358078603, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 26308; Mature: 26308

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKKKRVVVATAGTFLEWAEFTYYAYIANIMSGSFFPKLGNHLGLIATFSVFALSYFFR
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHH
PLGALFFGYLGDKMGRRVALQSSIMLMGLSSLCIGCLPAYDSIGILAPILLLIFRCVQGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCE
AVSGEFNGSAIYLIEHDSQKPCQAGSWTGFASALGMMFGGLMSTLIYLPHMPEWAWRIPF
EECCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LLGTLSCFCAMYFRKNLSESPPYLTLSNEKSSNPLKILFENYKKQLIRAVLLIAALT
HHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MENKKKRVVVATAGTFLEWAEFTYYAYIANIMSGSFFPKLGNHLGLIATFSVFALSYFFR
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHH
PLGALFFGYLGDKMGRRVALQSSIMLMGLSSLCIGCLPAYDSIGILAPILLLIFRCVQGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCE
AVSGEFNGSAIYLIEHDSQKPCQAGSWTGFASALGMMFGGLMSTLIYLPHMPEWAWRIPF
EECCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LLGTLSCFCAMYFRKNLSESPPYLTLSNEKSSNPLKILFENYKKQLIRAVLLIAALT
HHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]