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The map label for this gene is rlmN [H]

Identifier: 148359081

GI number: 148359081

Start: 1777948

End: 1779096

Strand: Reverse

Name: rlmN [H]

Synonym: LPC_0968

Alternate gene names: 148359081

Gene position: 1779096-1777948 (Counterclockwise)

Preceding gene: 148359082

Following gene: 148359080

Centisome position: 49.74

GC content: 36.64

Gene sequence:

>1149_bases
ATGGACCAACAAAAAGTGAATCTATTAAATTATAATTACTCGCAACTACGAGAATTATTGATGGCGTGGGATGAAAAACC
TTTCAGAGCTCAACAGCTATTTCAATGGATTCATCAAGTTGGCATACGTGACTTTGCTCAGATGACTAATTTAGGGAAAG
TGTTAAGAAATAAACTTTCCCAATTAGCTTGTATAGATTTACCTGAAATCGTTGCATGTCAAAAATCAGCTGATGGCACA
CATAAGTGGCTATTAAAGCTGGAGTGTGGAAATTGTATTGAAACCGTATTCATTCCGGAAGCAAATCGAGGGACACTTTG
TGTTTCATCACAAGTAGGTTGTGCTTTAAATTGTTCTTTTTGCTCCACAGCAAAGCAAGGATTTAATAGAAATTTATCAA
CAGCTGAAATAATTGGTCAAGTATGGTTGGCAGCACGAGAACTATCAGATAATAATGGCGCTCACGATAAGAAAATAACT
AACGTCGTGATGATGGGAATGGGTGAACCATTACTTAATTTCGATAATGTTGTTTCTGCTATGAGCATCATGATGGATGA
TTTAGCCTATGGATTATCCAAAAGACGGGTTACATTAAGTACTTCGGGTGTTTTACCAGAAATGGAACGACTCAGAGAAG
TCAGTCCTGTCGCGTTGGCGGTATCCTTACATGCACCTACAGATGAACTACGTAACGAGCTGGTACCAATTAATAAAAAA
TATCCTCTCTCCCAATTAATATCCCTATGTAAACGATATTTTAAAGACGAACCAAGAAGAAAAGTTACTTTTGAATACGT
CATGCTAAAAGGGGTTAATGATCAACCTGAACATGCTAGTCAATTAATTAAACTATTGCATAATGTGCCTGCAAAAGTTA
ATTTAATTCCTTTTAACCCATTTCCTTTAACTCAATATCAGAGATCATCGCGGGAAACAATTGATGCATTTCGAGATAAA
TTAATCAAGCATGGTATTAATACTATTACTCGCAAAACTCGTGGAGATGATATTGATGCAGCATGCGGCCAACTTGCTGG
CGAGGTAAAAGATAAAACCACCAGATCACAACGATGGCAAAAACTTCATTTCATGAGCAAAACAGATAAATCAACTGAAT
TGACCATTTCTTCGGAAGAAATTGCATAA

Upstream 100 bases:

>100_bases
TGCATGGTTCTGACAGCCTTGAAAATGCTGCTCGTGAAATAGCATTTTTCTTTGAACCACATGAGTTGTGCAATAGATAA
AGACAGGCTTGGGAGCGGTA

Downstream 100 bases:

>100_bases
CACAGTCTTGTATAATAACTGAGAGCCAAATAAGAACTTTCTCCAATATTCCTGGTTGGAGATTATGTATAAAAGGTATT
TTCACCTACAGACTCACATT

Product: Cfr family transporter radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MDQQKVNLLNYNYSQLRELLMAWDEKPFRAQQLFQWIHQVGIRDFAQMTNLGKVLRNKLSQLACIDLPEIVACQKSADGT
HKWLLKLECGNCIETVFIPEANRGTLCVSSQVGCALNCSFCSTAKQGFNRNLSTAEIIGQVWLAARELSDNNGAHDKKIT
NVVMMGMGEPLLNFDNVVSAMSIMMDDLAYGLSKRRVTLSTSGVLPEMERLREVSPVALAVSLHAPTDELRNELVPINKK
YPLSQLISLCKRYFKDEPRRKVTFEYVMLKGVNDQPEHASQLIKLLHNVPAKVNLIPFNPFPLTQYQRSSRETIDAFRDK
LIKHGINTITRKTRGDDIDAACGQLAGEVKDKTTRSQRWQKLHFMSKTDKSTELTISSEEIA

Sequences:

>Translated_382_residues
MDQQKVNLLNYNYSQLRELLMAWDEKPFRAQQLFQWIHQVGIRDFAQMTNLGKVLRNKLSQLACIDLPEIVACQKSADGT
HKWLLKLECGNCIETVFIPEANRGTLCVSSQVGCALNCSFCSTAKQGFNRNLSTAEIIGQVWLAARELSDNNGAHDKKIT
NVVMMGMGEPLLNFDNVVSAMSIMMDDLAYGLSKRRVTLSTSGVLPEMERLREVSPVALAVSLHAPTDELRNELVPINKK
YPLSQLISLCKRYFKDEPRRKVTFEYVMLKGVNDQPEHASQLIKLLHNVPAKVNLIPFNPFPLTQYQRSSRETIDAFRDK
LIKHGINTITRKTRGDDIDAACGQLAGEVKDKTTRSQRWQKLHFMSKTDKSTELTISSEEIA
>Mature_382_residues
MDQQKVNLLNYNYSQLRELLMAWDEKPFRAQQLFQWIHQVGIRDFAQMTNLGKVLRNKLSQLACIDLPEIVACQKSADGT
HKWLLKLECGNCIETVFIPEANRGTLCVSSQVGCALNCSFCSTAKQGFNRNLSTAEIIGQVWLAARELSDNNGAHDKKIT
NVVMMGMGEPLLNFDNVVSAMSIMMDDLAYGLSKRRVTLSTSGVLPEMERLREVSPVALAVSLHAPTDELRNELVPINKK
YPLSQLISLCKRYFKDEPRRKVTFEYVMLKGVNDQPEHASQLIKLLHNVPAKVNLIPFNPFPLTQYQRSSRETIDAFRDK
LIKHGINTITRKTRGDDIDAACGQLAGEVKDKTTRSQRWQKLHFMSKTDKSTELTISSEEIA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=356, Percent_Identity=56.1797752808989, Blast_Score=410, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 43217; Mature: 43217

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQQKVNLLNYNYSQLRELLMAWDEKPFRAQQLFQWIHQVGIRDFAQMTNLGKVLRNKLS
CCCCCCHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLACIDLPEIVACQKSADGTHKWLLKLECGNCIETVFIPEANRGTLCVSSQVGCALNCSF
HHHHCCCHHHHHHCCCCCCCEEEEEEEECCCEEEEEEECCCCCCCEEEECCCCCEEECHH
CSTAKQGFNRNLSTAEIIGQVWLAARELSDNNGAHDKKITNVVMMGMGEPLLNFDNVVSA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHH
MSIMMDDLAYGLSKRRVTLSTSGVLPEMERLREVSPVALAVSLHAPTDELRNELVPINKK
HHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHCCCCCCCC
YPLSQLISLCKRYFKDEPRRKVTFEYVMLKGVNDQPEHASQLIKLLHNVPAKVNLIPFNP
CCHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCC
FPLTQYQRSSRETIDAFRDKLIKHGINTITRKTRGDDIDAACGQLAGEVKDKTTRSQRWQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KLHFMSKTDKSTELTISSEEIA
HHHHHHCCCCCCEEEECHHCCC
>Mature Secondary Structure
MDQQKVNLLNYNYSQLRELLMAWDEKPFRAQQLFQWIHQVGIRDFAQMTNLGKVLRNKLS
CCCCCCHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLACIDLPEIVACQKSADGTHKWLLKLECGNCIETVFIPEANRGTLCVSSQVGCALNCSF
HHHHCCCHHHHHHCCCCCCCEEEEEEEECCCEEEEEEECCCCCCCEEEECCCCCEEECHH
CSTAKQGFNRNLSTAEIIGQVWLAARELSDNNGAHDKKITNVVMMGMGEPLLNFDNVVSA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHH
MSIMMDDLAYGLSKRRVTLSTSGVLPEMERLREVSPVALAVSLHAPTDELRNELVPINKK
HHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHCCCCCCCC
YPLSQLISLCKRYFKDEPRRKVTFEYVMLKGVNDQPEHASQLIKLLHNVPAKVNLIPFNP
CCHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCC
FPLTQYQRSSRETIDAFRDKLIKHGINTITRKTRGDDIDAACGQLAGEVKDKTTRSQRWQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KLHFMSKTDKSTELTISSEEIA
HHHHHHCCCCCCEEEECHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA