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The map label for this gene is ala [H]

Identifier: 148359043

GI number: 148359043

Start: 1736994

End: 1737830

Strand: Direct

Name: ala [H]

Synonym: LPC_0929

Alternate gene names: 148359043

Gene position: 1736994-1737830 (Clockwise)

Preceding gene: 148359042

Following gene: 148359044

Centisome position: 48.57

GC content: 36.08

Gene sequence:

>837_bases
ATGAATATCGCATTTGTTAACGGCAAGTATTGTTGTCAATCTGAAGCAAAAGTTTCAATATTTGATCGAGGTTTTCTTTT
TGGTGATTCGGTTTATGAAGTGCTGCCTGTTTACCATGGGCAGCCTTACTTTGTAGACCAACATCTTAACCGATTATTCT
CAAATATGAAAAAAATTAAGATGACTATACCAAATTATGATTGGCATGGTTTAATTCATAGACTAATATCAGAAAATAAT
GGAGGTAATTTACAAGTATATATCCAAGTCACACGAGGAAATCAAGGGGTGCGCAAGCATGATATCCCTACTTCCATCAC
ACCTTCTGTTATCGCATTCACCATGCATAATTCATTTCCCACCCTCGAAGATAAGGAACAGGGAATGTCAGCAAAACTGG
TTGAAGACTTTCGGTGGATGAGATGCGATATAAAAACTACTTCTTTAATTGCCAATATATTACTGAATGACGAGGCTGTA
TCTGCAGGATTCCATACTTCAATTCTTGCCCGGAATGGTCTAATTACAGAGGGAAGTAGTACCAACGTATTCATTGTCGC
CCAGGATGGCGTTATCAAGACCCCGCCTATGAATAATTTCTGTTTACCAGGAATTACGCGGCAAGTTGTTATTGAAATAA
TTAAAAAATTAGATTTAAAGTTCAGAGAAATAGAAATTAGTATTTCAGAGCTTTTTTCTGCTCAGGAAGTTTGGGTAACA
AGTACGACAAAAGAAGTATTCCCTATTACAAAGATTAATGATTCTTTGGTTAATAGCGGAAAAGTTGGCGAATATTGGCG
GATAATTAATGATTCTTACCAACAACTAGTAAACTAA

Upstream 100 bases:

>100_bases
TGCTTTGCAAACAGTGGAGTCAGGTGGATTCTATACCAGAACAAAAGATTCGATAAGGTTAGCATTTAAGCGATGGTTCG
GTTCCTAGGATATTTTCATA

Downstream 100 bases:

>100_bases
ATTATGACAAAAACGACTTTAATAGAATTCCCTTGCTATTTTCCTATAAAAATTATCGGCATTAACTCAGAATTATTGCT
CAATGAAATCAGGCAAATTG

Product: D-alanine-aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MNIAFVNGKYCCQSEAKVSIFDRGFLFGDSVYEVLPVYHGQPYFVDQHLNRLFSNMKKIKMTIPNYDWHGLIHRLISENN
GGNLQVYIQVTRGNQGVRKHDIPTSITPSVIAFTMHNSFPTLEDKEQGMSAKLVEDFRWMRCDIKTTSLIANILLNDEAV
SAGFHTSILARNGLITEGSSTNVFIVAQDGVIKTPPMNNFCLPGITRQVVIEIIKKLDLKFREIEISISELFSAQEVWVT
STTKEVFPITKINDSLVNSGKVGEYWRIINDSYQQLVN

Sequences:

>Translated_278_residues
MNIAFVNGKYCCQSEAKVSIFDRGFLFGDSVYEVLPVYHGQPYFVDQHLNRLFSNMKKIKMTIPNYDWHGLIHRLISENN
GGNLQVYIQVTRGNQGVRKHDIPTSITPSVIAFTMHNSFPTLEDKEQGMSAKLVEDFRWMRCDIKTTSLIANILLNDEAV
SAGFHTSILARNGLITEGSSTNVFIVAQDGVIKTPPMNNFCLPGITRQVVIEIIKKLDLKFREIEISISELFSAQEVWVT
STTKEVFPITKINDSLVNSGKVGEYWRIINDSYQQLVN
>Mature_278_residues
MNIAFVNGKYCCQSEAKVSIFDRGFLFGDSVYEVLPVYHGQPYFVDQHLNRLFSNMKKIKMTIPNYDWHGLIHRLISENN
GGNLQVYIQVTRGNQGVRKHDIPTSITPSVIAFTMHNSFPTLEDKEQGMSAKLVEDFRWMRCDIKTTSLIANILLNDEAV
SAGFHTSILARNGLITEGSSTNVFIVAQDGVIKTPPMNNFCLPGITRQVVIEIIKKLDLKFREIEISISELFSAQEVWVT
STTKEVFPITKINDSLVNSGKVGEYWRIINDSYQQLVN

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=281, Percent_Identity=26.6903914590747, Blast_Score=94, Evalue=7e-21,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 31500; Mature: 31500

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIAFVNGKYCCQSEAKVSIFDRGFLFGDSVYEVLPVYHGQPYFVDQHLNRLFSNMKKIK
CEEEEECCCEEECCCCEEEEEECCEEECCHHHHHHHHCCCCCEEHHHHHHHHHHHHEEEE
MTIPNYDWHGLIHRLISENNGGNLQVYIQVTRGNQGVRKHDIPTSITPSVIAFTMHNSFP
EECCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCC
TLEDKEQGMSAKLVEDFRWMRCDIKTTSLIANILLNDEAVSAGFHTSILARNGLITEGSS
CCCCHHCCCHHHHHHHHHHEEECCHHHHHHHHHHCCCCHHCCCCHHHHHHCCCEEECCCC
TNVFIVAQDGVIKTPPMNNFCLPGITRQVVIEIIKKLDLKFREIEISISELFSAQEVWVT
CEEEEEECCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEEEHHHHHCCCEEEEE
STTKEVFPITKINDSLVNSGKVGEYWRIINDSYQQLVN
ECCCHHEEEEECCHHHHCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIAFVNGKYCCQSEAKVSIFDRGFLFGDSVYEVLPVYHGQPYFVDQHLNRLFSNMKKIK
CEEEEECCCEEECCCCEEEEEECCEEECCHHHHHHHHCCCCCEEHHHHHHHHHHHHEEEE
MTIPNYDWHGLIHRLISENNGGNLQVYIQVTRGNQGVRKHDIPTSITPSVIAFTMHNSFP
EECCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCC
TLEDKEQGMSAKLVEDFRWMRCDIKTTSLIANILLNDEAVSAGFHTSILARNGLITEGSS
CCCCHHCCCHHHHHHHHHHEEECCHHHHHHHHHHCCCCHHCCCCHHHHHHCCCEEECCCC
TNVFIVAQDGVIKTPPMNNFCLPGITRQVVIEIIKKLDLKFREIEISISELFSAQEVWVT
CEEEEEECCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEEEHHHHHCCCEEEEE
STTKEVFPITKINDSLVNSGKVGEYWRIINDSYQQLVN
ECCCHHEEEEECCHHHHCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2644261; 2914916; 7626635; 9538014; 9749913 [H]