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The map label for this gene is dacA [H]

Identifier: 148359042

GI number: 148359042

Start: 1735689

End: 1736981

Strand: Direct

Name: dacA [H]

Synonym: LPC_0928

Alternate gene names: 148359042

Gene position: 1735689-1736981 (Clockwise)

Preceding gene: 148359030

Following gene: 148359043

Centisome position: 48.53

GC content: 37.51

Gene sequence:

>1293_bases
ATGCCATTTGGCCGCCTGCAAAAGGCTGTGATAAGATACATTTATTTTGCAACTATATCAGGTAATATCATGACCAGAGC
AACGTCTTTTCTATTGACGCTATTATTTTTTATTACTTTATTTGTACAGTCTACCAATTCAATGGCTGATGAAGTGCTCC
CCTCCTCTGATTTGCAGAAGCCTTCTCCAACAGTCACAACGAAACCTTTTACAACTCCAGCTGCTCCATTGCTTAATGCC
AAAGCTTATATTTTAATTGATGTGAATAGTGGAAAAGTAATTGCAGAAAAGAATAGTGAGGAAAAATTACCACCTGCCAG
TTTGACCAAAATGATGACATTGTATGTCATTTCTAATGCATTGAAGAGTGGACAAATTCATCTTAATGATTTGGTAAGAG
TGAGCCGTGATGCATGGCAGACAGGTGGCTCTCGTATGTTTATAAAAGAGGGTGATCAGGTCTCTGTTGAAGATCTTTTA
AAAGGCATTATAGTAGACTCTGGTAATGACGCTTGTGTCGCAATGGCAGAGCACCTTGGTGGTTCCGAAAATGCTTTTAC
AGACATTATGAATCAGCAAGCTCAAAATCTGGGTATGAAAAATAGTCATTTTACAGACAGTACTGGATTACCTGATCCTA
ATCTCTATACAACTGCTAAAGATTTGGCGATTTTAGGTCGAGCCTTAATACTTGATTTCCCTGAGTATTATGACTGGTAT
AAACAAAAATGGTTTACATATAACGGTATACGCCAACCTAATAGGAACCGTTTGTTGTGGCGAGATAGCCAAGTTGACGG
TATAAAAACAGGCCACACCAATGATGCTGGATATTGTCTAGTATCTTCCGCAAAGCGTAATAACATGCGTTTATTAGCTG
TGGTTTTAGGGGAACCCAGTGATTCTGTGCGTGCTGATGACAGTGAAAAATTGCTAAATTACGGATTTAGATTTTTTGAA
ACACATCAATTATATAAATCTGGGCAATCAATTGCCGAGTTACCGCTTTACAAAGGAACTTCTGATAAAGTTGCTGTGGG
TTTAACAGAAGATCAATTTATTACGATACCTGCCGGTCAATATGAAAGGTTAAATATTTCAACTAAAATTTCTAATGATT
TACAAGCTCCTATAAAGAAAGGCGATAAAGTTGGCGATATTATTGTGCAATTTAATAATAATATCGTTTCAAGCAAACCG
CTTTATGCTTTGCAAACAGTGGAGTCAGGTGGATTCTATACCAGAACAAAAGATTCGATAAGGTTAGCATTTAAGCGATG
GTTCGGTTCCTAG

Upstream 100 bases:

>100_bases
AGTTATTGCTAATATTACCAAAAAATAATAATTTTCGAAGTGCGATTAAATTAATTCACAACTTATTTAGCTTTATAAAA
AAAATATTAATATTTTTAAT

Downstream 100 bases:

>100_bases
GATATTTTCATAATGAATATCGCATTTGTTAACGGCAAGTATTGTTGTCAATCTGAAGCAAAAGTTTCAATATTTGATCG
AGGTTTTCTTTTTGGTGATT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6; PBP-6 [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MPFGRLQKAVIRYIYFATISGNIMTRATSFLLTLLFFITLFVQSTNSMADEVLPSSDLQKPSPTVTTKPFTTPAAPLLNA
KAYILIDVNSGKVIAEKNSEEKLPPASLTKMMTLYVISNALKSGQIHLNDLVRVSRDAWQTGGSRMFIKEGDQVSVEDLL
KGIIVDSGNDACVAMAEHLGGSENAFTDIMNQQAQNLGMKNSHFTDSTGLPDPNLYTTAKDLAILGRALILDFPEYYDWY
KQKWFTYNGIRQPNRNRLLWRDSQVDGIKTGHTNDAGYCLVSSAKRNNMRLLAVVLGEPSDSVRADDSEKLLNYGFRFFE
THQLYKSGQSIAELPLYKGTSDKVAVGLTEDQFITIPAGQYERLNISTKISNDLQAPIKKGDKVGDIIVQFNNNIVSSKP
LYALQTVESGGFYTRTKDSIRLAFKRWFGS

Sequences:

>Translated_430_residues
MPFGRLQKAVIRYIYFATISGNIMTRATSFLLTLLFFITLFVQSTNSMADEVLPSSDLQKPSPTVTTKPFTTPAAPLLNA
KAYILIDVNSGKVIAEKNSEEKLPPASLTKMMTLYVISNALKSGQIHLNDLVRVSRDAWQTGGSRMFIKEGDQVSVEDLL
KGIIVDSGNDACVAMAEHLGGSENAFTDIMNQQAQNLGMKNSHFTDSTGLPDPNLYTTAKDLAILGRALILDFPEYYDWY
KQKWFTYNGIRQPNRNRLLWRDSQVDGIKTGHTNDAGYCLVSSAKRNNMRLLAVVLGEPSDSVRADDSEKLLNYGFRFFE
THQLYKSGQSIAELPLYKGTSDKVAVGLTEDQFITIPAGQYERLNISTKISNDLQAPIKKGDKVGDIIVQFNNNIVSSKP
LYALQTVESGGFYTRTKDSIRLAFKRWFGS
>Mature_429_residues
PFGRLQKAVIRYIYFATISGNIMTRATSFLLTLLFFITLFVQSTNSMADEVLPSSDLQKPSPTVTTKPFTTPAAPLLNAK
AYILIDVNSGKVIAEKNSEEKLPPASLTKMMTLYVISNALKSGQIHLNDLVRVSRDAWQTGGSRMFIKEGDQVSVEDLLK
GIIVDSGNDACVAMAEHLGGSENAFTDIMNQQAQNLGMKNSHFTDSTGLPDPNLYTTAKDLAILGRALILDFPEYYDWYK
QKWFTYNGIRQPNRNRLLWRDSQVDGIKTGHTNDAGYCLVSSAKRNNMRLLAVVLGEPSDSVRADDSEKLLNYGFRFFET
HQLYKSGQSIAELPLYKGTSDKVAVGLTEDQFITIPAGQYERLNISTKISNDLQAPIKKGDKVGDIIVQFNNNIVSSKPL
YALQTVESGGFYTRTKDSIRLAFKRWFGS

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=367, Percent_Identity=48.5013623978202, Blast_Score=348, Evalue=5e-97,
Organism=Escherichia coli, GI1786851, Length=365, Percent_Identity=47.1232876712329, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI87082022, Length=358, Percent_Identity=38.5474860335196, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI87082054, Length=234, Percent_Identity=29.4871794871795, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 47867; Mature: 47736

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFGRLQKAVIRYIYFATISGNIMTRATSFLLTLLFFITLFVQSTNSMADEVLPSSDLQK
CCHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCC
PSPTVTTKPFTTPAAPLLNAKAYILIDVNSGKVIAEKNSEEKLPPASLTKMMTLYVISNA
CCCCEECCCCCCCCCHHHCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHH
LKSGQIHLNDLVRVSRDAWQTGGSRMFIKEGDQVSVEDLLKGIIVDSGNDACVAMAEHLG
HHCCCEEHHHHHHHHHHHHHCCCCEEEEECCCEECHHHHHHEEEEECCCCHHHHHHHHCC
GSENAFTDIMNQQAQNLGMKNSHFTDSTGLPDPNLYTTAKDLAILGRALILDFPEYYDWY
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHCCCHHHHHHH
KQKWFTYNGIRQPNRNRLLWRDSQVDGIKTGHTNDAGYCLVSSAKRNNMRLLAVVLGEPS
HHHCEEECCCCCCCCCCEEEECCCCCCEECCCCCCCCEEEEECCCCCCEEEEEEEECCCC
DSVRADDSEKLLNYGFRFFETHQLYKSGQSIAELPLYKGTSDKVAVGLTEDQFITIPAGQ
CCCCCCCHHHHHHHCHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEECCCCEEEECCCC
YERLNISTKISNDLQAPIKKGDKVGDIIVQFNNNIVSSKPLYALQTVESGGFYTRTKDSI
CEEEEEEECCCCHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEEEEECCCCCEEECCHHH
RLAFKRWFGS
HHHHHHHHCC
>Mature Secondary Structure 
PFGRLQKAVIRYIYFATISGNIMTRATSFLLTLLFFITLFVQSTNSMADEVLPSSDLQK
CHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCC
PSPTVTTKPFTTPAAPLLNAKAYILIDVNSGKVIAEKNSEEKLPPASLTKMMTLYVISNA
CCCCEECCCCCCCCCHHHCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHH
LKSGQIHLNDLVRVSRDAWQTGGSRMFIKEGDQVSVEDLLKGIIVDSGNDACVAMAEHLG
HHCCCEEHHHHHHHHHHHHHCCCCEEEEECCCEECHHHHHHEEEEECCCCHHHHHHHHCC
GSENAFTDIMNQQAQNLGMKNSHFTDSTGLPDPNLYTTAKDLAILGRALILDFPEYYDWY
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHCCCHHHHHHH
KQKWFTYNGIRQPNRNRLLWRDSQVDGIKTGHTNDAGYCLVSSAKRNNMRLLAVVLGEPS
HHHCEEECCCCCCCCCCEEEECCCCCCEECCCCCCCCEEEEECCCCCCEEEEEEEECCCC
DSVRADDSEKLLNYGFRFFETHQLYKSGQSIAELPLYKGTSDKVAVGLTEDQFITIPAGQ
CCCCCCCHHHHHHHCHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEECCCCEEEECCCC
YERLNISTKISNDLQAPIKKGDKVGDIIVQFNNNIVSSKPLYALQTVESGGFYTRTKDSI
CEEEEEEECCCCHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEEEEECCCCCEEECCHHH
RLAFKRWFGS
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3279397; 8905232; 9278503; 7042389 [H]