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The map label for this gene is rluB [H]

Identifier: 148358978

GI number: 148358978

Start: 1660270

End: 1661016

Strand: Direct

Name: rluB [H]

Synonym: LPC_0863

Alternate gene names: 148358978

Gene position: 1660270-1661016 (Clockwise)

Preceding gene: 148358977

Following gene: 148358979

Centisome position: 46.42

GC content: 41.37

Gene sequence:

>747_bases
ATGAGCAGTGAAAGATTACAGAAAATATTAAGTCAAGCCGGACTTGGATCTCGTCGTGAAATGGAGCGGTGGATTGATAA
TGGTTGGGTGCAGGTTAATGGCAAACCAGCTAAACTGGGAGATTCCGCTGGCCCTAGTGATAAAATTAGCGTCAAAGGAA
AGTTGATTCCCAACCCTTTAAAAGGCAAACAAAACATCCGGGTTCTTCTTTACCATAAACCGGTCGGTGAAGTATCCAGT
CGACATGATCCAAAATTTGAAAAAACAGTATTTGATAATTTACCGCATCTTAGACAGGGGCGTTGGGTGCAAGTGGGGCG
CCTGGATTTAAATACTTCCGGTTTGCTCATTTTTACTAATAATGGCGAATTGGCTAACCAACTCATGCATCCCAAATATG
GTTTGGAAAGAGAGTATGCCGTACGAGTTCATGGTCAAGTCAGCCAGGAATCTTTAAATAACTTATTGAAAGGGGTTATG
TTGGATGACGGTTTGGCAAAATTTACCAAAATTCAATTTCGTGGGGGAGAAGGAACAAATTCCTGGTACCATGTGACCCT
TAATGAAGGAAGAAACAGAGAAGTACGTCGGTTGTGGGAGTCTCAAGGTGTTGAGGTTAGTCGATTAATAAGAATCCGTT
ATGGTATGCTTACGATGCCCAGATTTTTATCCAGAGGGCAGTTTTATGAACTTACCCCAAAAGAAGTGACTGAATTTCTT
GATTCATTACCCAACGAATTGTCTTAA

Upstream 100 bases:

>100_bases
TTTAACTTAAATTACTTAAGTGAACTTCCTCCCTTACCTGAAATAATGGATACATTAACCCTGCACGAGGCAACCCAACC
TATAAAAGAGTGTACGCAAA

Downstream 100 bases:

>100_bases
ATGATTTAAAAACGGATGTTGGATTTTGTTGTATTCACTAAAAGAATCATAATTTCATGGGCAGTTGTTTTTGATTAATT
ATATTTTTGTGAGAGATGAT

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MSSERLQKILSQAGLGSRREMERWIDNGWVQVNGKPAKLGDSAGPSDKISVKGKLIPNPLKGKQNIRVLLYHKPVGEVSS
RHDPKFEKTVFDNLPHLRQGRWVQVGRLDLNTSGLLIFTNNGELANQLMHPKYGLEREYAVRVHGQVSQESLNNLLKGVM
LDDGLAKFTKIQFRGGEGTNSWYHVTLNEGRNREVRRLWESQGVEVSRLIRIRYGMLTMPRFLSRGQFYELTPKEVTEFL
DSLPNELS

Sequences:

>Translated_248_residues
MSSERLQKILSQAGLGSRREMERWIDNGWVQVNGKPAKLGDSAGPSDKISVKGKLIPNPLKGKQNIRVLLYHKPVGEVSS
RHDPKFEKTVFDNLPHLRQGRWVQVGRLDLNTSGLLIFTNNGELANQLMHPKYGLEREYAVRVHGQVSQESLNNLLKGVM
LDDGLAKFTKIQFRGGEGTNSWYHVTLNEGRNREVRRLWESQGVEVSRLIRIRYGMLTMPRFLSRGQFYELTPKEVTEFL
DSLPNELS
>Mature_247_residues
SSERLQKILSQAGLGSRREMERWIDNGWVQVNGKPAKLGDSAGPSDKISVKGKLIPNPLKGKQNIRVLLYHKPVGEVSSR
HDPKFEKTVFDNLPHLRQGRWVQVGRLDLNTSGLLIFTNNGELANQLMHPKYGLEREYAVRVHGQVSQESLNNLLKGVML
DDGLAKFTKIQFRGGEGTNSWYHVTLNEGRNREVRRLWESQGVEVSRLIRIRYGMLTMPRFLSRGQFYELTPKEVTEFLD
SLPNELS

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=250, Percent_Identity=56, Blast_Score=265, Evalue=2e-72,
Organism=Escherichia coli, GI1790453, Length=247, Percent_Identity=32.7935222672065, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1788510, Length=239, Percent_Identity=27.6150627615063, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI87081838, Length=185, Percent_Identity=34.0540540540541, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 28308; Mature: 28177

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSERLQKILSQAGLGSRREMERWIDNGWVQVNGKPAKLGDSAGPSDKISVKGKLIPNPL
CCHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEEEECEECCCCC
KGKQNIRVLLYHKPVGEVSSRHDPKFEKTVFDNLPHLRQGRWVQVGRLDLNTSGLLIFTN
CCCCCCEEEEEECCCHHHCCCCCCCHHHHHHHCCCCHHCCCEEEEEEEEECCCEEEEEEC
NGELANQLMHPKYGLEREYAVRVHGQVSQESLNNLLKGVMLDDGLAKFTKIQFRGGEGTN
CCHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCC
SWYHVTLNEGRNREVRRLWESQGVEVSRLIRIRYGMLTMPRFLSRGQFYELTPKEVTEFL
CEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHH
DSLPNELS
HHCCCCCC
>Mature Secondary Structure 
SSERLQKILSQAGLGSRREMERWIDNGWVQVNGKPAKLGDSAGPSDKISVKGKLIPNPL
CHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEEEECEECCCCC
KGKQNIRVLLYHKPVGEVSSRHDPKFEKTVFDNLPHLRQGRWVQVGRLDLNTSGLLIFTN
CCCCCCEEEEEECCCHHHCCCCCCCHHHHHHHCCCCHHCCCEEEEEEEEECCCEEEEEEC
NGELANQLMHPKYGLEREYAVRVHGQVSQESLNNLLKGVMLDDGLAKFTKIQFRGGEGTN
CCHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCC
SWYHVTLNEGRNREVRRLWESQGVEVSRLIRIRYGMLTMPRFLSRGQFYELTPKEVTEFL
CEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHH
DSLPNELS
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]